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Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 00 S. W. 23 rd Drive, Gainesville, Florida 32608, US Jerome A. Hogsette

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Page 1: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Evaluation of Fly Traps Under Desert Conditions

USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE)

1600 S. W. 23rd Drive, Gainesville, Florida 32608, USA

Jerome A. Hogsette

Page 2: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

House Fly Trap Evaluation in Cairo

Attraction of Moisture-Deprived House Flies to Dry Baits

House Fly Ultraviolet Light Trap Studies

2009 Projects

Stable Fly Repellent Studies

Page 3: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Cairo, 2009: No project proposals from foreign scientists were approved by the Egyptian Ministry of Health .

Page 4: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Off the job…

Page 5: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Evaluation of BugJammer traps for trapping house flies at the Vaccine

Institute, Cairo, Egypt

Jerome A. Hogsette1, Hanafi A. Hanafi2, Ulrich R. Bernier1, Daniel L. Kline1, Emad Y. Fawaz2, Barry D.

Furman2, and David F. Hoel3

1USDA-AS, CMAVE, Gainesville, FL, 2NAMRU-3, Cairo, Egypt,

3Program Manager, Medical Entomology Collaborations, Navy Marine Corps Public Health

Center Detachment, CMAVE, Gainesville, FL

Page 6: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

BugJammer traps - evaluated in Aswan in 2008 against sand flies.

Page 7: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

What is the BugJammer Trap?

Housing covered by a sticky sleeve.

Speaker

PVC tube with electronics inside

Base

Power

Page 8: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Underneath the speaker are 4 ‘D’ cell batteries to power a microchip…

…on which is recorded the heartbeat of a dog in a

continuous loop.

Page 9: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

To compare the efficacy of the BugJammerfly traps, with and without sound, with

the standard Captivator fly trapsbaited with the Farnam fly bait.

Performance of fly traps is dependent on geographic location and

environmental conditions.

Although they have been designed as a trap for stable flies, BugJammer

traps will attract and capture house flies outdoors as well as indoors.

Objective:

Hypothesis:

Military Relevance:

Page 10: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Vaccine Institute

Page 11: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Trap placement

Page 12: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

1. The traps were rotated through the 3 trap sites.

2. The traps were left at each site for 24 hours, then flies were counted.

4. Tests were replicated 3 times.

3. Captivator trap was emptied and recharged with water and bait. Clean sticky paper was put on the Bug Jammer traps.

Experimental design:2009

Three traps – BugJammer on, BugJammer off, Captivator with bait.

Page 13: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

The wet jar trap (Farnam Captivator) captured significantly more house flies.

Mean = 5958.9 (± 905.8)a

BugJammer

Mean = 1322.8 (± 377.2)b

2009Results

Captivator

Bugjammer Sound On:

Mean = 1383.7 (± 341.0)b

Bugjammer Sound Off:

Page 14: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

However, it appears that the BugJammer trap may have captured the maximum number of flies it could hold within the 24-hr test period.

Page 15: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE)

1600 S. W. 23rd Drive, Gainesville, Florida 32608, USA

Jerome A. Hogsette

House Fly Ultraviolet Light Trap Studies

Page 16: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

UV Light traps

Page 17: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

To compare the effects of housing design on fly attraction and capture.

Trap housings that allow for maximum exposure of fluorescent tubes will

attract and catch the most flies.

UV light traps used by the military should provide the highest

level of control possible.

Objective:

Hypothesis:

Military Relevance:

Page 18: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

How does design affect efficacy?

Page 19: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

First Component

1. Housing: Open front or closed front.

Page 20: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

First Component

1. Housing: Open front vs restricted open front.

Page 21: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Second Component

2. Main attractant: UV light.

Currently, all tubes used in light traps are standard models.

Standard UV tubes – 350 – 370 nm.

Black light blue tubes – 350 nm and higher.

Page 22: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Third Component

3. Glue Boards: With or without pheromone.

Page 23: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Traps about 90 cm above the floor.

Room size: 3.3 by 6.0 m.

Tube type/trap housing type/glue board typecombinations rotated after every test.

Experimental Design

Page 24: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Fifty house flies counted and released.

Counts made 1, 4, and 24 hours after flies were released.

Experimental Design

Page 25: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Open grid housing – 15.3 ± 1.8a

Closed grid housing – 13.5 ± 1.5a

Results

Page 26: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

BL tube in open grid housing – 20.2 ± 2.6a

BLB tube in open grid housing – 10.3 ± 1.7b

Results

Page 27: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

BL tube in closed grid housing – 13.6 ± 2.3a

BLB tube in closed grid housing – 13.5 ± 1.9a

Results

Page 28: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Glue board with pheromone – 17.7 ± 3.1a

Glue board without pheromone – 22.7 ± 4.2a

Glue board with pheromone – 7.7 ± 1.8b

Glue board without pheromone – 13.0 ± 2.6b

Results

Open grid housing and BL tube with:

Open grid housing and BLB tube with:

Page 29: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Glue board with pheromone – 15.8 ± 2.5c

Glue board without pheromone – 11.3 ± 3.6c

Glue board with pheromone – 12.2 ± 1.2d

Glue boards without pheromone – 14.8 ± 3.7d

Results

Closed grid housing and BL tube with:

Closed grid housing and BLB tube with:

Page 30: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

Grid type Tube type Glue board type

Open – 15.3 ± 1.8a

Closed – 13.5 ± 1.5a

BL – 20.2 ± 2.6a

BLB – 10.3 ± 1.7b

BL – 13.6 ± 2.3c

BLB – 13.5 ± 1.9c

P – 17.7 ± 3.1a

P – 7.7 ± 1.8b

N – 22.7 ± 4.2a

N – 13.0 ± 2.6b

P – 15.8 ± 2.5c

P – 12.2 ± 1.2d

N – 11.3 ± 3.6c

N – 14.8 ± 3.7d

Results

Page 31: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

NSN for the Terminator trap.

Similar trap/bait evaluations are planned in other geographical locations in different climatic zones, eg. Northern Australia and Peru.

Future Direction:

Technology Transfer:Manuscripts in preparation.

Page 32: Evaluation of Fly Traps Under Desert Conditions USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology (CMAVE) 1600 S. W. 23 rd Drive, Gainesville,

The End