Evaluation of human Platelet Lysate in Animal-Free Conditions

for Cell Culture and Cytotoxicity Testing

Alexander Edwards1, Christopher Longmore1, Lottie Roscoe1, Carol Treasure1.1XCELLR8 Ltd, The Innovation Centre, Sci-Tech Daresbury, Cheshire WA4 4FS, UK

Human Platelet Lysate (hPL) is commonly used as an

animal-free alternative to FBS/FCS. Currently, Human

Serum is used for animal-free cell culture conditions at

XCellR8, as we aim to replace the use of animal derived

products in the laboratory. Human Platelet Lysate is

comparable on price to Human Serum. The aim of this work

was to investigate the use of hPL for animal-free cell culture

systems in comparison with Human Serum for both cell

growth, viability and functional cytotoxicity testing.

Methods

Human Platelet Lysate supported comparable levels of TK6 and Fibroblast cell proliferation

and cell viability at all test concentrations compared with Human Serum. Cell morphology

was unchanged between test and control conditions for both TK6 (not shown) and

Fibroblast cells. The results for functional cytotoxicity testing using TK6 showed that 5% and

2.5% hPL produced similar performance compared with 10% human serum. For

Fibroblasts, 2% hPL gave the most comparable results compared with 2% HS. In

conclusion, hPL offers a viable and cost effective alternative to HS for cell culture models.

RESULTS

*p<0.05 vs. 10% HiHs (t-test). n=6. Data from 2 independent experiments.

*p<0.05 vs. 10% HiHs (t-test). Data from 2 independent experiments shown; n=3 per experiment.

*p<0.05 vs. 10% HiHs (t-test). PC data from 2 independent experiments shown; n=12 per experiment. One representative experiment for NC shown, not significant in 2 independent experiments.

*p<0.05 vs. 10% HiHs (t-test). n=6. Data from 2 independent experiments.

*p<0.05 vs. 10% HS (t-test). Data from 2 independent experiments shown; n=3 per experiment.

*p<0.05 vs. 10% HS (t-test). PC data from 2 independent experiments shown; n=12 per experiment. One representative experiment for NC shown, not significant in 2 independent experiments.

Gluc-TK6 (Lymphoblastoid)

HDFn-XF (Fibroblast)

*p<0.05 vs. 10% HS (t-test). n=6. Data from 2 independent experiments.

*p<0.05 vs. 10% HS (t-test). n=6. Data from 2 independent experiments.

Morphology of Fibroblasts over a time course: Human Serum (2%) vs hPL (2, 1, 0.5%).

INTRODUCTION / AIM

Cell Growth

Cell Viability

Functional Cytotoxicity Testing

Functional Cytotoxicity TestingCell Growth

Cell Viability

Morphology

DISCUSSION