evanescent wave sensing

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Molecular beacon fluorescence detection of pseudogene SHMT-psI using two molecular beacon probes, E8/E10 and E9/E10

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Molecular beacon fluorescence detection of pseudogene SHMT- psI using two molecular beacon probes, E8/E10 and E9/E10. Evanescent Wave Sensing. Detection of Phosphorylated STAT3 Protein by Fiber-Based Sensor. Fluorescence Generated at Surface. Exciting Laser. Cladding. Core. - PowerPoint PPT Presentation

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Page 1: Evanescent Wave Sensing

 

Molecular beacon fluorescence detection of pseudogene SHMT-psI using two molecular beacon probes, E8/E10 and E9/E10

Page 2: Evanescent Wave Sensing

Evanescent Wave Sensing

Page 3: Evanescent Wave Sensing

Cladding

Core

Exciting Laser

Fluorescence Generated at Surface

Fluorescence coupled into fiber.

Fluorophore labeled Phospho-STAT3 antibodies.

Phospho-STAT3

Phospho-STAT3 antibodies (IgG)

400 450 500 550 600 650 7000

300

600

900

1200

1500

Flu

ore

sce

nce

inte

nsi

ty in

a.u

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Wavelength in nm

Evanascent wave excited Alexa-430 Spectra in two different probes.

Signal from stimulated Cells Signal from non-stimulated Cells

Detection of Phosphorylated STAT3 Protein by Fiber-Based Sensor

STAT - Signal Transducer and Activator of Transcription protein

Page 4: Evanescent Wave Sensing

Kretschmann (ATR) geometry used to excite surface plasmons

Surface Plasmon Sensors

Page 5: Evanescent Wave Sensing

Surface plasmon resonance curves. The left-hand side curve is for just the silver film (labeled Ag); the right-hand side shows the curve (labeled Ag/p-4-BCMU) shifted on the deposition of a monolayer Langmuir-Blodgett film of poly-4-BCMU on the silver film

Page 6: Evanescent Wave Sensing

 

Close packing of colloidal nanospheres to form a photonic crystal of close-packed colloidal array. (Left) Atomic Force Microscope (AFM) image of the surface layer. (Right) Scanning Electron Microscope (SEM) image of a cross-section (Markowicz and Prasad; unpublished).

Photonic Crystal Sensors

Page 7: Evanescent Wave Sensing

 

Visible extinction spectra showing how diffraction depends on the glucose concentration for a 125µm-thick PCCA glucose sensor. The ordinate is given as –log T, where T is the transmittance. The PCCA expands for concentrations between 0.1 and 0.5 mM glucose.

Page 8: Evanescent Wave Sensing

ACIS, November 2003

Basic concepts:- Use molecularly specific fluorescent tags to label cells

- Single-file flow of cells (hydrodynamic focusing)

- Laser illumination of single cells

- Multiparameter fluorescence detection

-Data analyses and/or sorting

FLOW CYTOMETRY

Page 9: Evanescent Wave Sensing

Schematics of a five parameter flow cytometer showing details of collection scheme.

Page 10: Evanescent Wave Sensing

ACIS, November 2003

Various parameters monitored

Page 11: Evanescent Wave Sensing

ACIS, November 2003

Clusters of cells represent distinct populations of cells

Page 12: Evanescent Wave Sensing

Applications

• HIV Monitoring• Leukemia or Lymphoma Immunophenotyping • Organ Transplant Monitoring• DNA Analysis for Tumor Ploidy and SPF• Primary and Secondary Immunodeficiency• Bacterial contamination in food and water

Page 13: Evanescent Wave Sensing

Biophotonics Opportunities

• Modeling of light propagation in tissue• In-vivo Bioimaging, Spectroscopy, and Optical Biopsy• Real-Time monitoring of Drug Interactions • Optical Biopsy• Nano-Biophotonic Probes• Multiphoton Processes for Biotechnology• Femtolaser based biotechnology\• Nanoarray multianalytic sensors• Multiparameter Molecular Flowcytometry• Single Molecule Biofunctions• Nanomedicine

Page 14: Evanescent Wave Sensing

Acknowledgements

Researchers at the Institute:Prof. E. BergeyProf. A. CartwrightProf. M. SwihartProf. E. FurlaniDr. A. KachynskiDr. A. KuzminDr. Y. SahooDr. H. PudavarDr. T. OhulchanskyyDr. D. BharaliDr. D. LuceyDr. K. BabaDr. J. Liu

DURINT/AFSOR Dr. Charles Lee

Outside CollaboratorsProf. R.BoydProf. J.HausProf. J M J FrechetProf. M. StachowiakDr. A. OseroffDr. R. PandeyDr. J. MorganDr. P Dandona

Page 15: Evanescent Wave Sensing

“Lighting the Way to Technology through Innovation”

The Institute for Lasers, Photonics and Biophotonics

University at Buffalo

Biophotonics

P.N.Prasad

www.biophotonics.buffalo.eduwww.biophotonics.buffalo.edu