Evolution in a changing environment: the genetic architecture of adaptation outside centers of domestication of Phaseolus vulgaris and P. coccineus

Roberto Papa

Università Politecnica delle Marche, Ancona

FYI - FEFAC, COCERAL and Copa-Cogeca event on Leguminoses in the framework of the international year of pulses on 29th November 2016 in

the European Parliament

GOL(16)10445

Phaseolus vulgaris L.

Independent

domestications

Mesoamerican

gene pool

Northern Peru

and Ecuador

Andean

gene pool

From northern Mexico to Northwestern Argentina;

Altitudes ranging from 500 to 2000 m. asl;

Rainfall from 500 to 1800 mm.

Evolution in a changing environment: the

genetic architecture of adaptation outside

centers of domestication of P. vulgaris and P. coccineus. Coordinator R. Papa, UNIVPM

GbS on 11500 AccessionsResequencing 560 genotypes

Transcriptomics and Metabolomics Population genomicsAdmixture mapping

GWAS

Genes for adaptation

Deep genomic and phenotypic characterization of the whole collection and Haplotype reconstruction (BeanHapMap)

10.000

500

200

GbS 10,000 pure lines from landraces (Pv_ALL)

Phenotypes MLFT

ACTIVITIES and OUTCOMES

single seed purified accessions50% from America and50% from Europe

Phenotypes controlledconditions

Metabolomics

Transcriptomics

WGS 10x

• An immortal collection of pure lines in P. vulgaris

• Complete molecular and phenotypic characterization

and Computed haplotypes for all the collection

• Genes controlling adaptation (signature of selection, traits-

environmental variable association, expression analysis, validation of

candidates genes)

MAIN DELIVERABLES

expanding the scope and the impact

ASSOCIATED PARTNERS

Collaborative network

cooperation and association to specific activities

Phenotyping (field trials, seed traits, nutritional value, abiotic and biotic stress, roots)

Genetic resources and germplasmGenomic information and molecular data

DisseminationEx situ conservation of 10,000 pure lines

…