ex. 8 : aseptic technique – inoculating different types of media
DESCRIPTION
Ex. 8 : Aseptic Technique – Inoculating Different Types of Media. Key concepts: Different types of bacterial cultures: broth, agar slant, and agar deep. Aseptic transfer of bacteria from one type of culture medium to another . Appropriate use of inoculating loops and needles. - PowerPoint PPT PresentationTRANSCRIPT
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Ex. 8: Aseptic Technique – Inoculating Different Types of Media
Key concepts: 1. Different types of bacterial cultures: broth,
agar slant, and agar deep.2. Aseptic transfer of bacteria from one type
of culture medium to another.3. Appropriate use of inoculating loops and
needles.
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Four Basic Forms of Bacterial Culture Media
Agar plateBroth Agar Deep Agar Slant
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Always use sterile instruments to transfer bacteria!
Loop and Needle
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Flame loop before AND after using it
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Other Sterile Transfer Instruments
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“Little Finger Technique”
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NA Broth
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NA Slant Cultures
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Agar Deeps: What is happening?
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Use the Inoculating Needle to inoculate a deep