excellent ed maps can be obtained by mad phasing
DESCRIPTION
Excellent ed maps can be obtained by MAD phasing. Electrophoretic mobility. Frictional coefficient. Polyacrylamide gel. Use fresh ammonium persulfate. Discontinuous buffer system. SDS-PAGE. Stacking gel. Resolving gel. log Mr. SDS-PAGE. 1. pI (isoelectric focusing). 2 Mr (SDS-PAGE). - PowerPoint PPT PresentationTRANSCRIPT
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Excellent ed maps can be obtained by MAD phasing.
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Electrophoretic mobility
Frictional coefficient
Polyacrylamide gel
Use fresh ammonium persulfate.
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SDS-PAGE
Stacking gelResolving gel
Discontinuous buffer system
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1. pI (isoelectric focusing)
2 Mr (SD
S-PAGE)
log Mr
SDS-PAGE
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MWCO of dialysis tubing
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DEAE- CM-
affinity column
Concanavalin A
Gel filtration column: long
Ion exchange column: medium
Affinity column: small
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Sedimentation velocity
Centrifugal force
Sedimentation coefficient 1 S=10-13 sec
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Mass under nondenaturing conditions
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ESI Mass Spectrum
MALDI TOFMatrix Assisted
Laser Desorption Ionization
Time-of-Flight
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One cycle of Edman degradation
Mixture of
PTH-aa’s
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Performic acid oxidation and cleavage of -S-S-
N-terminal sequencing
Degenerate oligomer primer
cDNA cloning
DNA sequencing
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4 Ca2+ binding motifs in calmodulin
Hybridoma cell
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ELISA
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Western blotting
Imaging plate (IP) is 50-times more sensitive than X-ray film.
Actin filaments
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Gold cluster-conjugated antibody
Secondary structure content
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Low resolution High
resolution
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Spin=1/2
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When R < 5 A
R
Chemical shift
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X-ray vs NMR
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DCC
항이뇨성의
Natural
Synthetic
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Merrifield solid-phase peptide synthesis