excellent ed maps can be obtained by mad phasing

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Excellent ed maps can be obtained by MAD phasing.

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Excellent ed maps can be obtained by MAD phasing. Electrophoretic mobility. Frictional coefficient. Polyacrylamide gel. Use fresh ammonium persulfate. Discontinuous buffer system. SDS-PAGE. Stacking gel. Resolving gel. log Mr. SDS-PAGE. 1. pI (isoelectric focusing). 2 Mr (SDS-PAGE). - PowerPoint PPT Presentation

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Page 1: Excellent ed maps can be  obtained by MAD phasing

Excellent ed maps can be obtained by MAD phasing.

Page 2: Excellent ed maps can be  obtained by MAD phasing

Electrophoretic mobility

Frictional coefficient

Polyacrylamide gel

Use fresh ammonium persulfate.

Page 3: Excellent ed maps can be  obtained by MAD phasing

SDS-PAGE

Stacking gelResolving gel

Discontinuous buffer system

Page 4: Excellent ed maps can be  obtained by MAD phasing

1. pI (isoelectric focusing)

2 Mr (SD

S-PAGE)

log Mr

SDS-PAGE

Page 5: Excellent ed maps can be  obtained by MAD phasing

MWCO of dialysis tubing

Page 6: Excellent ed maps can be  obtained by MAD phasing

DEAE- CM-

affinity column

Concanavalin A

Gel filtration column: long

Ion exchange column: medium

Affinity column: small

Page 7: Excellent ed maps can be  obtained by MAD phasing

Sedimentation velocity

Centrifugal force

Sedimentation coefficient 1 S=10-13 sec

Page 8: Excellent ed maps can be  obtained by MAD phasing

Mass under nondenaturing conditions

Page 9: Excellent ed maps can be  obtained by MAD phasing

ESI Mass Spectrum

MALDI TOFMatrix Assisted

Laser Desorption Ionization

Time-of-Flight

Page 10: Excellent ed maps can be  obtained by MAD phasing
Page 11: Excellent ed maps can be  obtained by MAD phasing

One cycle of Edman degradation

Mixture of

PTH-aa’s

Page 12: Excellent ed maps can be  obtained by MAD phasing
Page 13: Excellent ed maps can be  obtained by MAD phasing
Page 14: Excellent ed maps can be  obtained by MAD phasing

Performic acid oxidation and cleavage of -S-S-

N-terminal sequencing

Degenerate oligomer primer

cDNA cloning

DNA sequencing

Page 15: Excellent ed maps can be  obtained by MAD phasing
Page 16: Excellent ed maps can be  obtained by MAD phasing

4 Ca2+ binding motifs in calmodulin

Hybridoma cell

Page 17: Excellent ed maps can be  obtained by MAD phasing

ELISA

Page 18: Excellent ed maps can be  obtained by MAD phasing

Western blotting

Imaging plate (IP) is 50-times more sensitive than X-ray film.

Actin filaments

Page 19: Excellent ed maps can be  obtained by MAD phasing

Gold cluster-conjugated antibody

Secondary structure content

Page 20: Excellent ed maps can be  obtained by MAD phasing
Page 21: Excellent ed maps can be  obtained by MAD phasing

Low resolution High

resolution

Page 22: Excellent ed maps can be  obtained by MAD phasing

Spin=1/2

Page 23: Excellent ed maps can be  obtained by MAD phasing

When R < 5 A

R

Chemical shift

Page 24: Excellent ed maps can be  obtained by MAD phasing

X-ray vs NMR

Page 25: Excellent ed maps can be  obtained by MAD phasing

DCC

항이뇨성의

Natural

Synthetic

Page 26: Excellent ed maps can be  obtained by MAD phasing

Merrifield solid-phase peptide synthesis