Simple, Rapid Isolation of DNA from Tough-To-Lyse Fungi & Bacteria

KitInput

Sample wet weight

Bacterial cells

Yeast cells Soft Tissue

ZR Fungal/Bacterial DNA MicroPrep™ 10 - 20 mg 2 x 108 2 x 107 40 mg

ZR Fungal/Bacterial DNA MiniPrep™ 50 - 100 mg 109 108 200 mg

ZR Fungal/Bacterial DNA MidiPrep™ 250 - 500 mg 5 x 109 5 x 108 500 mg

ZR-96 Fungal/Bacterial DNA Kit™ 10 - 20 mg/well 2 x 108/well 2 x 107/well 40 mg/well

ZR Fungal/Bacterial DNA Kits

P Isolate PCR-quality DNA from fungi and bacteria

P Efficient processing with ultra- high density BashingBeads™

P Does not require the use of organic denaturants or lytic enzymes (e.g., Zymolyase)

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1-888-882-96821-714-288-96821-714-288-9643info@zymoresearch.comwww.zymoresearch.com

The ZR Fungal/Bacterial DNA MicroPrep™, ZR Fungal/Bacterial DNA MiniPrep™, ZR Fungal/Bacterial DNA MidiPrep™, and ZR-96 Fungal/Bacterial DNA Kit™ are designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedures are easy and can

be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads™. Fast-Spin column or plate technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.

Our state of the art BashingBeads™ are constructed of the highest quality, most dense ceramic material available today. They are

used when thorough sample homogenization/lysis is required by the researcher. DNA shearing by physical and chemical methods is minimized

since the beads are fracture resistant and chemically inert. They are unique amongst the lysis matrices offered by other companies for DNA isolation from

tough-to-lyse materials.

DNA isolated from S. cerevisiae (spores) and E. coli using the ZR Fungal/Bacterial DNA MiniPrep™ is high quality and structurally intact. Equivalent amounts of yeast and bacteria were processed using the ZR Fungal/Bacterial DNA MiniPrep™ or the kit from Supplier M. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The size marker is a 1 kb ladder (Zymo Research).

YeastSpores E. coli

ZymoResearch

E. coli

Supplier M

YeastSpores1 kb

Specifications

Sample SourcesGram (+) bacteria, Gram (-) bacteria, Yeast, Filamentous fungi, Unicellular algae, Filamentous algae, Protists

DNA Binding Capacities ZR Fungal/Bacterial DNA MicroPrep™: 5 µg/prep.ZR Fungal/Bacterial DNA MiniPrep™: 25 µg/prep.ZR Fungal/Bacterial DNA MidiPrep™: 125 µg/prep.ZR-96 Fungal/Bacterial DNA Kit™: 5 µg/well.

Processing TimesZR Fungal/Bacterial DNA MicroPrep™: 10 min.ZR Fungal/Bacterial DNA MiniPrep™: 10 min.ZR Fungal/Bacterial DNA MidiPrep™: 20 min.ZR-96 Fungal/Bacterial DNA Kit™: 40 min.

DNA PurityA260/A280 > 1.8

Note - ™ Trademarks of Zymo Research Corporation. This product is intended for research use only and should only be used by trained professionals. This product is not intended for use in diagnostic procedures. Some reagents included with this kit are irritants. Wear protective gloves and eye protection. Follow the safety guidelines and rules enacted by your research institution or facility.

Ordering Information

1. Add sample to a ZR BashingBead™ Lysis Tube & process in a cell disruptor at maximum speed for 5 min.

2. Centrifuge the ZR BashingBead™ Lysis Tube at 10,000 x g for 1 min.

3. Transfer 400 µl supernatant to a Zymo-Spin™ IV Spin Filter in a Collection Tube & centrifuge at 7,000 rpm for 1 min.

4. Add 1.2 ml Fungal/Bacterial DNA Binding Buffer to the filtrate in the Collection Tube from Step 3.

5. Transfer 800 µl of the mixture from Step 4 to a Zymo-Spin™ IIC Column in a Collection Tube & centrifuge at 10,000 x g for 1 min. Discard the flow through & repeat.

6. Add 200 µl DNA Pre-Wash Buffer to the Zymo-Spin™ IIC Column & centrifuge at 10,000 x g for 1 min., then wash w/ 500 µl Fungal/Bacterial DNA Wash Buffer.

7. Transfer the column to a clean 1.5 ml microcentrifuge tube & add 100 µl DNA Elution Buffer directly to the column matrix. Centrifuge at 10,000 x g for 30 sec. to elute the DNA.

ZR Fungal/Bacterial DNA MiniPrep™ Short Protocol

Product Description Catalog No. Kit SizeZR Fungal/Bacterial DNA MicroPrep™ D6007 50 preps.ZR Fungal/Bacterial DNA MiniPrep™ D6005 50 preps.ZR Fungal/Bacterial DNA MidiPrep™ D6105 25 preps.ZR-96 Fungal/Bacterial DNA Kit™ D6006 2 x 96 preps.

ZR Fungal/Bacterial DNA Kits

PCR Ready, Ultra-pure DNA

OR

Centrifuge & Transfer Lysate to Zymo-Spin™ V-E w/ Zymo-Midi Filter™

SpinWashElute

Pulverize Samplein ZR BashingBead™ Lysis/Filtration Tube

MidiPrep Format

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$

$

$ $

((( Pulverize Sampleswith ZR BashingBead™ Lysis Rack

96-well Block

Bind, Wash, Elute DNAwith Silicon-A™ Plate$

$

$

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PCR Ready, Ultra-pure DNA

96-Well Format

Filter Lysate with Zymo-Spin™ IV

Bind, Wash, Elute DNA with Zymo-Spin™ IC or IIC

Pulverize Samplewith ZR BashingBead™ Lysis Tube

$

$

$

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PCR Ready, Ultra-pure DNA

MicroPrep & MiniPrep Formats

OR