extraction of bioactive compounds by using cinnamomum iners
TRANSCRIPT
Evaluation of Anti-Malaria Potency of Various Extracts of Cinnamomum iners
Bark
Norasheila Binti Mohd Saad
152167
BSc. (H)-Industrial Chemistry
Introduction: Pusat PenyelidikanDadah & Ubat- Ubatan
• First established on recommendation by World Health Organization (WHO) in 1973
• Selected as one of the Higher Institutions' Centres of Excellence (HICoE) by the Ministry of Higher Education.
• Established Memorandum of Understanding (MoU) with Pharmaniaga Berhad
• Provide consultancy services to advise on the optimization of analytical, extraction process and the identification of phytocompounds for the Kacip Fatimah Project.
• Vision
• Leading in drug research
• Mission
• To conduct relevant, innovative and quality research
• To disseminating new kind of useful knowledge to serve mankind
Introduction: Cinnamomum iners
• Local name : • Kayu Manis Hutan
• English name : Wild Cinnamom
• Diagnostic characters :
• Leaves pink when young
• Glaucous beneath with three longitudinal veins
• Crushed fresh leaves and inner bark with strong smell of cinnamon
• Fruit green with yellow spots when young, dark blue when ripen
Project
• Extraction process by using soxhletmethod
• Used 4 types of solvents; petroleumether, dichloromethane, methanol andwater
• Extraction of the tree’s bark
• Trunk straight, bark smooth, greyish-brown, inner bark pinkish with strongfragrant smell.
• The timber is insect resistent and usedfor house building and cabinet work.
• The bark yields an inferior grade ofcinnamon but oil distilled from it andfrom the leaves can be used forflavouring and for incense sticks.
Method
Grinding • The bark was grinded by using grinder
Extraction • Soxhlet method
Analysis•GC-MS
• IR
• Enzyme assay
• Paper chromatography
Grinder
Extraction
25 g of dried, grinded C. iners bark was weighed and transferred into a
thimble
The soxhlet apparatus was set up; apparatus
consisted of chiller at the top, soxhlet in the middle and round bottom flask at
the bottom
A few anti bumping chips were added into the round bottom flask
Solvent was added until the round bottom flask was half full. ~600ml
Chiller and heat was turned on and the
temperature was set
Solvent was dried by using rotary evaporator
Procedure was repeated by using
dichloromethane, ethanol and deionized water
Analysis: GC-MS
Analysis: IRSolvent: Petroleum ether
Solvent: Dichloromethane
Solvent: Ethanol
Solvent: Water
Analysis: Enzyme assay
Analysis: Paper chromatography
95:5 80:20
Experience/ knowledge
• I obtained precious experiences regarding on how toconduct research like a postgrad student
• Basically, all related more to organic chemistry, subcategory of natural products.
• Lab technicians explained step by step procedures onhow to prepare the samples, analyze them and also howto interpret the results.
• All the information obtained are hard to find atsomewhere else.
Conclusion
• The industrial training at here is very fun and exciting.
• This is because the students are exposed on how tohandle the machine ourselves and at the same time newprecious experiences are obtained.
• The Center provides good facilities and moderninstruments.
• Thus, faster results can be attained.
• This helps to save a lot of time and also give preciseresults.
Comment/suggestion
• By only using GC-MS, FT-IR, enzyme assay and paperchromatography, the identities of the compounds arestill not well known.
• Thus, further analysis should be done in order to revealmore the structure and activity of the compounds.
• This Center is a good place for students to do internshipor postgrad, especially for people that are interested inorganic chemistry and research field.
• The students must be high discipline and able to workindependently.
THANK YOU