extraction of gasoline from soil samples using supercritical fluid extraction and gc-ms
DESCRIPTION
Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS. By: Tim Abell and Zach Bensley. Purpose. To create a method to extract gasoline from soil samples. . Importance. Test contamination of soil after an oil spill. - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/1.jpg)
Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS
By: Tim Abell and Zach Bensley
![Page 2: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/2.jpg)
Purpose
• To create a method to extract gasoline from soil samples.
![Page 3: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/3.jpg)
Importance
• Test contamination of soil after an oil spill.• To see if clean up from an oil spill was
successful.
![Page 4: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/4.jpg)
Procedure
• Sample Preparation (SFE):– Approximately 1.00 g of celite spiked with 0.50 mL
of gasoline.– 3 samples were prepared.
• Placed spiked sample in SFE column.• 50 mL of chloroform in beaker in an ice bath
to capture the extracted gasoline.• Extracted each sample for 30 minutes.
![Page 5: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/5.jpg)
Procedure
• Sample Preparation (GC-MS):– Created Standard with 0.50 mL of gasoline
and 100 mL chloroform.– Each sample mixture was diluted to 100 mL with
chloroform.• Ran standard and 3 sample on GC-MS.
![Page 6: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/6.jpg)
Data
• Standard
![Page 7: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/7.jpg)
Data
• Celite Trial 1
![Page 8: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/8.jpg)
Data
• Celite Trial 2
![Page 9: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/9.jpg)
Data
• Celite Trial 3
![Page 10: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/10.jpg)
Procedure
• Sample Preparation (SFE):– Obtained soil from outside.– Approximately 5.00 g of soil with 0.50 mL gasoline.– 3 samples were prepared.
• Placed spiked soil samples in SFE column.• Placed 50.0 mL chloroform in a beaker in an
ice bath.• Ran each sample for 30 minutes.
![Page 11: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/11.jpg)
Procedure
• Sample Preparation (GC-MS):– Created Standard with 0.50 mL of gasoline
and 100 mL chloroform.– Each sample mixture was diluted to 100 mL with
chloroform.• Ran standard and 3 sample on GC-MS.
![Page 12: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/12.jpg)
Data
• Soil Sample Trial 1
![Page 13: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/13.jpg)
Data
• Soil Sample Trial 2
![Page 14: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/14.jpg)
Data
• Soil Sample Trial 3
![Page 15: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/15.jpg)
Error
• Making soil sample more fine.• Longer run time on SFE for soil samples.• Gasoline and chloroform mixture sat for 6
days.
![Page 16: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/16.jpg)
Conclusion
• Gasoline can be extracted using SFE and condensed in chloroform.
• More work with the method of extraction with soil samples.
![Page 17: Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS](https://reader035.vdocument.in/reader035/viewer/2022062218/56816185550346895dd11cb8/html5/thumbnails/17.jpg)
Future Work
• Test with different solvents.• Extract non-spiked soil samples.