extraction of nicotine
DESCRIPTION
Extraction of Nicotine . By: DJ Hall and Brittany Hicks. Purpose. Determine if nicotine is extractable by means of supercritical fluid Run more than one sample type to determine if this makes a significant difference. SFE. Supercritical Fluid Extraction Uses high pressure CO 2 - PowerPoint PPT PresentationTRANSCRIPT
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Extraction of Nicotine By: DJ Hall and Brittany Hicks
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PurposeDetermine if nicotine is extractable by means of
supercritical fluidRun more than one sample type to determine if
this makes a significant difference
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SFESupercritical Fluid Extraction
Uses high pressure CO2
Used to extract organics from different types of matrixes
Allows for easy separation
ProblemsAll soluble organics will be separated form matrix
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GC-MSGas Chromatography Mass Spectroscopy
Separation of violate organicsDetermine the analyte separated at specific
retention times using MS
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Nicotine (162.12g/mol)Poison (parasympathomimetic alkaloid)Formed in nightshade family of plantsAntiherbivor chemicalSmall doses
StimulantRelaxation
Large dosesFatal
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Extraction2 Cigarettes Marlboro blend 272 Pieces Nicorette Gum 2mg50 Drops of e-cigarette liquid 2.4% by weight
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Extraction cont.All extractions were obtained by bubbling into
methanol and using SFE methanol modifier pumpCigarettes
Ground using mortar and pestle
Nicorette GumCut into small pieces
E-cigarette liquidCelite as inert matrix
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Determination of NicotineAll samples were diluted to 50ml of methanolA standard was created by diluting 4mg of
Nicotine in 50ml of methanolStandards and Samples were run using the GC-MS
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Reference Mass Spectra
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Standard
Nicotine peak
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Cigarettes
Nicotine peak
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Cigarettes cont.
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E-Cigarette Liquid
Nicotine peak
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E-Cigarette Liquid Cont.
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Nicorette Gum
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Chromatogram ComparisonE-Cigarette Liquid
Nicorette Gum
Cigarette
Standard
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ConclusionIt is possible to extract nicotine using the SFEBoth the E-Cigarette and Cigarette samples
contained nicotineThe Nicorette Gum showed undetectable amounts
of nicotineNicotine may have be contained in the gum residue
that was filtered before dilution
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Future ProjectQuantify the amounts of nicotine extractedDetermine the extraction efficiency
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Work CitedFischer, M. and Jefferies, T. Optimization of
Nicotine Extraction from Tobacco Using Supercritical Fluid Technology with Dynamic Extraction Modeling. J.Agric. Food Chem. (1996), 44, 1258-1264.