F3c F2c F1c B1 B2 B3

F3 F2 F1 B1c B2c B3c

A

B

C

Primer annealing

Stem-loop structure and loop primers

Inverted repeats concatamers

LF

LFc

LBc

LB

LB

FIPBIP

LF

FIP

BIP

F3

B3

B2F1c B1

F1 B1c

F2c

B2c F1 B1c

F1c B1

F2

Supplemental Data Figure 1. LAMP amplification mechanismScheme of the amplification mechanism of LAMP reaction. F3, B3, FIP and BIP primers anneal on the target in the showed positions (A) and by the peculiar amplification and displacement mechanism they are responsible for the formation of the “dumb-bell” starting structure, from which the whole reaction starts in an exponential-like fashion. Additional loop primers (LF and LB) allow an increase in the amplification rates by annealing on single strand regions of the loops in the dumb-bell structure (B). Concatamers made by repetitions of the initial module are produced (together with other structures) during the reaction