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FACULTY OF BIOTECHNOLOGY AND ANIMAL HUSBANDRY

LIST OF COURSES FOR EXCHANGE STUDENTS Academic year: 2016/2017

Course title Person responsible for the course Semester ECTS points

General genetics Dr inż. Daniel Polasik winter/ summer

4

Biotechnology and genetic engineering Dr hab. inż. Arkadiusz Terman winter/ summer

5

Animal microbiology Dr inż. Karol Fijałkowski winter/ summer

5

Fundamentals of laboratory diagnostics Dr hab. inż. Agnieszka Tomza-Marciniak winter/ summer

5

Industrial enzymology Dr inż. Radosław Drozd winter/ summer

5

Protéomique Dr hab. inż. Malgorzata Ożgo winter/ summer

5

Proteomics Dr inż. Agnieszka Herosimczyk winter/ summer

5

Animal Physiology Dr inż. Katarzyna Michałek winter/ summer

5

Biotechnologie der Fortpflanzung von Nutztieren Dr hab. inż. Bogdan Lasota, prof. ZUT winter/ summer

4

Cellular engineering in animal reproduction Dr inż. Tomasz Stankiewicz winter/ summer

4

Genomics Dr inż. Daniel Polasik winter/ summer

5

Molecular biology Dr hab. inż. Arkadiusz Terman winter/ summer

5

Genetic engineering methods Dr hab. inż. Arkadiusz Terman winter/ summer

5

Molecular modeling of enzymes Dr inż. Radosław Drozd winter/ summer

5

Food and nutrition in relation to human health Dr hab. inż. Arkadiusz Pietruszka winter/ summer

5

Medical and veterinary microbiology Dr inż. Karol Fijałkowski winter/ summer

5

Environmental toxicology Dr hab. inż. Agnieszka Tomza-Marciniak winter/ summer

5

In vitro and in vivo methods in toxicological assessment of xenobiotics

Dr hab. inż. Agnieszka Tomza-Marciniak winter/ summer

5

Cellular engineering in animal reproduction Dr inż. Tomasz Stankiewicz winter/ summer

5

Methods of monitoring the reproductive processes in animals Dr inż. Tomasz Stankiewicz winter/ summer

4

Animal embryology Dr inż. Tomasz Stankiewicz winter/ summer

4

Genetic markers for food quality Dr inż. Daniel Polasik winter/ summer

5

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Course title GENERAL GENETICS

Teaching method Lecture/laboratory

Person responsible for the course

Daniel Polasik, PhD E-mail address to the person responsible for the course

[email protected]

Course code (if applicable)

ECTS points 4

Type of course Obligatory Level of course bachelor

Semester Winter/Summer Language of instruction English

Hours per week 2 Hours per semester 30

Objectives of the course Get knowledge about inheritance of traits and modern techniques using in molecular genetics

Entry requirements Basics of molecular biology and biochemistry

Course contents

Lectures. History of genetics. Terms using in genetics. Inheritance of quantitative and qualitative traits. Structure of DNA and chromosomes. Genes and genetic code. Mutations and other reasons of biodiversity. Genes expression and their regulation. Genetic and physical maps of genome. Population genetics. Laboratories. DNA analysis methods and their practical use.

Assessment methods Writing test + project work

Recommended readings

1. E. Passarge "Color Atlas of Genetics", Thieme Medical Publishers, 2007. 2. B. Lewin "Genes VII", Oxford University Press, 2000. 3. P.C. Winter, G.I. Hickey, H.L. Fletcher, "Instant Notes in Genetics" Bios Scientific Publishers Limited,

2003.

Additional information Maximum laboratory group size - 15

Course title BIOTECHNOLOGY AND GENETIC ENGINEERING



Arkadiusz Terman, D.Sc. E-mail address to the person responsible for the course

[email protected]


ECTS points 5

Type of course Obligatory Level of course Bachelor/master

http://ebooklibrary.thieme.com/SID0000000000000/ebooklibrary/flexibook/pubid-2087011021/show_pdf.html?/pdf/pubid-2087011021_1000.pdf

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Semester Summer/Winter Language of instruction English


Objectives of the course Get knowledge about genetics and molecular biotechnology methods

Entry requirements Molecular biology and genetics

Course contents

The course discusses current knowledge about the generic engineering in biotechnology and molecular mechanisms. The course include: genomic DNA isolation, RNA isolation, primers digestion, PCR analysis, use of RFLP, AFLP, RAPD and RT-PCR methods, restriction enzymes analysis, agarose gel electrophoresis, gene expression analysis, practical use of different software for genomes analysis.

Assessment methods Writing test + presentation

Recommended readings 1. T. Brown “Genomes 3”, Garland Publishing , 2006 2. G. Gibson, S. Muse “A Primer of Genome Science, Third Edition”, Sinauer Associates Inc., 2009


Course title ANIMAL MICROBIOLOGY



Karol Fijałkowski PhD E-mail address to the person responsible for the course

[email protected]


ECTS points 5


Semester Winter, summer Language of instruction English


Objectives of the course

Aims of the Course The course aims are to provide a comprehensive theoretical and practical knowledge of medical and veterinary microbiology microbiology and to provide students with the latest information in scientific microbiology. The student should be able to: Discuss the classification of bacteria; Describe the bacterial colony and cell structure; Explain the bacterial physiology and growth; Discuss the bacterial genetics; Describe in details the morphology, the culture, spread, biochemical activities, antigenic characters, pathogenesis, laboratory diagnosis; Define the organs commonly involved in the infection; Explain the methods of microorganisms controls.

4

Entry requirements Basic lab knowledge and skills. Ability to pipet, make solutions and dilutions and to execute protocols which require the use of sterile techniques.

Course contents

Information about working in microbiological laboratory; Methods for determination and controlling bacterial growth; Methods of culturing bacteria; Preparing and storage of culture media; Conditions of bacterial cultures; Microscopic examination of microorganisms; Procedure for preparing bacterial smears; Staining procedures; Detection and identification of various kind of microorganisms; Study of biochemical activity of microorganisms; Determination of antibiotic susceptibility of pathogenic bacteria; Identification of virulence factors produced by microorganisms; Molecular diagnostics of microorganisms – PCR based techniques; Isolation and identification of microorganisms from environmental samples and animal material and determination of their quantity; Isolations of pure bacteria from a mix culture; Validation of methods in microbiology laboratory.

Assessment methods Grade, project work, continuous assessment

Recommended readings 1. L. M. Prescott, “Microbiology”, McGraw-Hill Science, 2002. 2. C. L. Gyles, J. F. Prescott, J. G. Songer, C. O. Thoen “Pathogenesis of Bacterial Infections in Animals

4th Ed”, Blackwell Publishing, 2010.

Additional information

Course title FUNDAMENTALS OF LABOLATORY DIAGNOSTICS

Teaching method laboratory


Agnieszka Tomza-Marciniak, PhD E-mail address to the person responsible for the course

[email protected]


ECTS points 5

Type of course optional Level of course master

Semester Winter/summer Language of instruction English

Hours per week 2h Hours per semester 30h

Objectives of the course The course aims to provide students with knowledge of laboratory tests check a sample of blood, urine, feaces and body tissues.

Entry requirements physiology, animal anatomy

5

Course contents

Complete Blood Count (CBC) test. Automated and semi-automated blood analysis. The urine analysis (Reader Urine Analyser). The physico-chemical and microscopic properties of the urine. Urine sample collection and analysis. Urine sediment analysis. The qualitative and quantitative methods in parasitology. Coproscopic techniques for detection and quantitative estimation of endoparasites. Microscopic Examination. The post-mortem parasitological examination: dissection, parasites isolation, preservation and examination of collected samples. Cryptosporidium detection methods. Zielh-Nielsen staining method: preparation and microscopic examination. Cryptosporidium immunochromatographic test. Trichinella detection: trichinoscopy and pooled-sample digestion method.

Assessment methods continuous assessment

Recommended readings 1. Pagana K., Pagana T., 2006: Mosby's Diagnostic and Laboratory Test Reference. Elsevier Health

Sciences. 2. Garcia L., 2009: Practical Guide to Diagnostic Parasitology. American Society for Microbiology.

Additional information size of the group – max. 10 students

Course title INDUSTRIAL ENZYMOLOGY



Radoslaw Drozd, PhD E-mail address to the person responsible for the course

[email protected]


ECTS points 5




Objectives of the course Get basic knowledge in the field of enzymology and methods for selection and modification of enzymes for use on an industrial scale

Entry requirements Basics of chemistry, biophysics, biochemistry, physical chemistry

Course contents

Basic of enzymology, enzymes kinetic constants determination. Prediction of structure and function of enzymes. Isolation, selection and purification appropriate enzymes for industrial process. Methods for enhancing and protection an activity and stability enzymes in industrial processes. Immobilization of enzymes. Design and optimization selected industrial process with using the enzymes.

Assessment methods Project work

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1. Athel Cornish-Bowden “Fundamentals of enzyme kinetics” Portland Press, 2004 2. Jeffrey Cleland, Charles Craik. “Protein engineering: principles and practice” Wiley-Liss, 1996 3. Lilia Alberghina”Protein engineering in industrial biotechnology” Harwood Academic Publishers, 2000 4. Wolfgang Aehle”Enzymes in industry: production and applications” Wiley-VCH, 2004 5. Julio Polaina, Andrew MacCabe “Industrial enzymes: structure, function and applications” Springer-

Verlag, 2007


Course title PROTÉOMIQUE

Teaching method Cours théoriques et exercices en laboratoire


Malgorzata Ozgo D.Sc. E-mail address to the person responsible for the course

[email protected]


ECTS points 5

Type of course Optionnel Level of course Bachelor/master/doctoral

Semester Eté/Hiver Language of instruction FranÇais



Connaissances théoriques et pratiques des techniques de séparation des protéines basées sur gel et chromatographiques

Capacité des participants à utiliser des outils bioinformatiques avancés pour analyser les données protéomiques (gels 1D et 2D, spectres de masse)

Utilisation pratique du MS MALDI-TOF (spectromètre de masse matrix-assisted laser desorption/ionisation time of flight) pour l'identification de protéines

Entry requirements Bases en biologie cellulaire et en biochimie des protéines.

Course contents

Introduction à la protéomique. Importance biologique des modifications post-transcriptionnelles et post-translationnelles des protéines. Organisation du protéome. Les principes généraux de l'analyse protéomique. Techniques de séparation des protéines basées sur gel. Les composants de la matrice du gel de séparation. Electrophorèse en gel de polyacrylamide contenant du dodécylsulfate de sodium (SDS-PAGE), le principe et les applications de l'électrophorèse sur gel natif PAGE. Electrophorèse bidimensionnelle (2-DE) – principe de la méthode, préparation des échantillons pour la 2-DE, bandes d'IPG (IPG strips), focalisation isoélectrique. Méthodes de détection des protéines: coloration au Bleu de Coomassie, coloration à l'argent, coloration inverse avec des ions (cuivre, zinc), autoradiographie, fluorographie, coloration fluorescente. Analyse différentielle sur un gel unique (two-dimensional difference in gel electrophoresis 2D-DIGE) – principe et applications de la méthode. Acquisition d'image et analyse de gels 1D et 2D. Logiciels d'analyse des gels 1D et 2D. Applications de la spectrométrie de masse (MS) pour l'identification des protéines. Méthodes d'ionisation en spectrométrie de masse. Types d'analyseurs de masse. Cartographie peptidique massique (peptide mass fingerprinting PMF). Méthodes chromatographiques pour la séparation des protéines. Chromatographie en phase liquide (LC). Chromatographie liquide bidimensionnelle (2D LC). Les stratégies protéomiques basées sur la chromatographie liquide : LC-MS, LC-MS/MS, LC-MS/MS multidimensionnelle. Chromatographie d'affinité. Types de protéomique : structurelle, fonctionnelle et clinique.

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Assessment methods Evaluation continue du travail en laboratoire

Examen écrit à la fin du cours


1. Sheehan D., Tyther R. (Ed.). Two-dimensional electrophoresis protocols. Humana Press, New York, 2009.

2. Garfin D., Ahuja S. (Ed.). Handbook of isoelectric focusing and proteomics. Elsevier Academic Press, Amsterdam, 2005.

3. Heftmann E. (Ed.). Chromatography, sixth edition. Elsevier Academic Press, Amsterdam, 2004. 4. Walker J.M. (Ed.), second edition. The proteomics protocols handbook. Humana Press, New Jersey,

2002. 5. Rabilloud T. (Ed.). Proteome research: two-dimensional gel electrophoresis and identification

methods. Springer, Berlin, 2000. 6. Hames B.D. (Ed.), third edition. Gel electrophoresis of proteins: a practical approach. Oxford

University Press, England, 1998.


Course title PROTEOMICS

Teaching method Lectures and laboratory exercises


Agnieszka Herosimczyk PhD E-mail address to the person responsible for the course

[email protected]


ECTS points 5

Type of course Obligatory Level of course Bachelor/master/doctoral

Semester Summer/winter Language of instruction English



The purpose of teaching is to learn and understand:

biological significance of proteome organization

significance of protein identification

significance of identification of differences between protein profiles

selected proteomic techniques

actual trends in proteomic researches

Entry requirements Basic of biology and biochemistry.

8

Course contents

Introduction to proteomics. Genome, transcriptome, proteome. Biological significance of post-transcriptional and post-translational protein modifications. Proteome organization. Proteome analysis and protein identification. Sample preparation. The purpose of proteome analysis and protein identification, cellular lysis, lysis buffers (chaotropic agents, detergents, reducing agents, amfolites), methods of sample purification, methods of protein precipitation. Protein separation techniques. The general principles of proteomic analysis. Basic components of polyacrylamide gels, proteins detection, methods of staining proteins. One-dimensional gel electrophoresis, stacking gels, resolving gels, SDS-PAGE electrophoresis, native PAGE electrophoresis, technique of preparation and usage of mini gels. Electrophoresis – 2D. Rehydration significance, principles of isoelectric focusing, isoelectric point, rehydratation buffers, IPG balancing strips, migration buffers, second- dimension of 2D-SDS PAGE electrophoresis. Western blotting. Transfer of proteins from gel to membrane. Wet transfer, semi-dry transfer. Immunodetection. Mass Spectrometry (MS) application in proteomics. Maldi-ToF mass spectrometry - Matrix-Assisted Laser Desorption/Ionization (MALDI), Time-of-Flight (TOF) mass spectrometry, proteolytic enzymes usage in sample preparation. Bioinformatic tools. Bioinformatic programs: Quantity One, PDQuest, BioTool. Usage of this programs for proteome analysis. Quantity and quality proteome analysis. Bioinformatic databases, protein identification principles, Peptide Map Fingerprinting –PMF. Proteomics in Poland and in the World. Current progress in proteomic studies in the world and their practical application. The purpose of proteomics. HUPO – Human Proteome Organization.

Assessment methods To receive a credit for a course in proteomics, students have to get positive mark in a test.

Recommended readings 1. Walker J.M. (Ed.). The Proteomics Protocols Handbook. Softcover. 2005. 2. Liebler D.C. Introduction to proteomics. Tools for the new biology. Humana Press. 2002. 3. Palzkill T. Proteomics. Kluwer Academic Publishers. 2000.


Course title ANIMAL PHYSIOLOGY

Teaching method Lecture / Laboratory Exercises


Katarzyna Michałek PhD E-mail address to the person responsible for the course

[email protected]


ECTS points 5

Type of course Obligatory Level of course Bachelor/master/doctoral

Semester Summer/Winter Language of instruction English



To develop the general understanding of the fundamental processes of the animal body system

To learn about how animals adapt physiologically to environmental challenges.

To develop the ability to think critically about issues in animal physiology.

Entry requirements Basics of the biology and the physiology

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Course contents

Introduction to Animal Physiology. Definition of comparative animal physiology. General features of a central nervous system. Neurons and synapses. Propagation of the nervous impulse in vertebrates. The autonomic nervous system (the sympathetic and parasympathetic system). The receptors. Cutaneous receptors. Proprioceptors. The excitation of receptors. Sense organs. The general composition of the blood. The red blood corpuscles. Structure and role of haemoglobin. The white blood corpuscles (granulocytes, monocytes and lymphocytes). The blood platelets. Composition and role of the blood plasma. Oxygen transport in blood. Carbon dioxide transport in blood. The coagulation of the blood. Blood groups. Circulatory system. General features of the circulation. Pulmonary circulation. Systemic circulation. Physiology of the mammalian heart. Cardiac muscle. The properties of cardiac muscle. The sequence of events in the cardiac cycle. The nervous regulation of the heart. Respiration. Mammalian lungs. The mechanics of the respiratory movements. Lung volume. Exchange of gases in the lungs. Exchange gases in the tissues. The control of the respiratory movements. The respiratory centers. The temperature and heat balance of the body. Endothermy and heterothermy. Regulation of body temperature. The regulation of heat production and loss. Behavioral temperature regulation. Hibernation and estivation. Hyperthermia, hyperthermia and fever. The physiology of digestion. Digestion in the oral cavity. The secretion of saliva. Digestion in the stomach. Gastric juice. The actions of gastric juice. The secretion of gastric juice. Intestinal digestion. The pancreatic juice. Properties of pancreatic juice. The liver and bile. Bile storage and expulsion. Functions of the small and the large intestine. The absorption of the foodstuffs. Ruminates. Renal excretion. The composition and characters of the urine. Structure and functions of the kidneys (the secretion of urine and hormones, regulation of the extracellular homeostasis). Functions of the glomeruli and the renal tubules. The regulation of the renal functions.

Assessment methods Continuous assessment of the laboratory work

Written test at the end of the course


1. C. Etchberger, M. Nordie, J. Fowles, P. Munn. Principles of human physiology. Addison-Wesley Longman. 2004.

2. C. Ladd Prosser (Ed.). Comparative animal physiology. New York: Wiley-Liss. 1991. 3. C.L. Stanfielsd, W.J.German. Principles of human physiology. Pearson Education. 2008. 4. K. Schmidt-Nielsen. Animal physiology: adaptation and environment. Cambridge University Press. New

York. 1997. 5. P. Carew Withers. Comparative animal physiology. Saundersa College Pub. 1992. 6. R. W. Hill, G.A. Wyse, M. Anderson. animal Physiology. Sinauer Associates, Incorporated. 2008.


Course title BIOTECHNOLOGIE DER FORTPFLANZUNG VON NUTZIEREN

Teaching method Vorlesungen, Seminare, Übungen


Bogdan Lasota E-mail address to the person responsible for the course

[email protected]


ECTS points 4

Type of course obligatorisch Level of course master

Semester Sommer/Winter Language of instruction Deutsch


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Nach dem Kursabschluss soll der Student die Grundlagen der Reproduktionssteuerung von weiblichen Nutztieren sowie Spermakonservierungsmethoden bei verschiedenen Nutztierarten kennen. Der Student soll einen Überblick über die neuesten biotechnischen Methoden bekommen (In-Vitro-Befruchtung, Embryotransfer- und -konservierung). Der Student soll die Kenntnisse über das Klonen von Tieren sowie über die Möglichkeiten der Anwendung von Stammzellen erwerben. Auch die EU-Gesetzgebung betreffs der fortgeschrittenen Biotechniken soll bekannt werden.

Entry requirements Grundlagen der Physiologie

Course contents

Gewinnung von Oozyten aus Ovarfollikeln mit verschiedenen Methoden. Kriterien der Beurteilung der Oozytenqualität. Geräte und Material zur Embryonengewinnung Assisted Reproductive Technology, Methoden der Embryonengewinnung. Einstufung der Embryonen anhand der morphologischen Beurteilung. Physikalische und chemische Prozesse bei der Kriokonservierung von Embryonen. Kriokonservierungsmethoden von Embryonen landwirtschaftlichen Nutztieren und Labortieren. Anwendung der Kriokonservierung von Embryonen in der Praxis. Kriokonservierung von Oozyten. Anwendung von biotechnischen Methoden bei der Regulierung und Steuerung des Sexualzyklus bei weiblichen Tieren. Induktion der Polyovulation. Klonen von Säugetieren. Möglichkeiten der Anwendung von Klonen. Gesetzgebung und Vorschriften zum Umsatz mit Oozyten, Embryonen und Stammzellen in der EU.

Assessment methods Testat mit einer Note

Recommended readings 1. Niemann H., Ehling Ch., Falge R., 1997,Biotechnological methods for preservation and maintaince of

farm animals genetic resources, Schriftenreihe des BML “Angewandte Wissenschaft” 465, 65-76. 2. Kauffold P., Thamm I., Zustandsbeurteilung von Rinderembryonen, FZfTP Dummerstorf-Rostock, 1985


Course title CELLURAL ENGINEERING IN ANIMAL REPRODUCTION



Tomasz Stankiewicz, PhD Barbara Błaszczyk, D.Sc.

E-mail address to the person responsible for the course

[email protected] [email protected]


ECTS points 4

Type of course (compulsory/obligatory) Level of course master



mailto:[email protected]


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Student knows the most important facts and achievements in the field of cellular engineering in mammalian reproduction. He knows the factors that determine the reproductive potential of mammals. Student specifies and describes methods of cellular engineering used in manipulations on gametes and embryos. Student knows methods of embryos collection and transfer. He describes potential benefits of embryo transfer in animal husbandry. Students know how to acquire and assess the quality of gametes. He can carried out sperm capacitation and set up the cultures in procedures IVM, IVF and IVC. Students knows how to evaluate the quality of embryos. He can correctly schedule of embryo transfer procedure. Student is aware of the importance of the knowledge. He knows the advantages and limitations associated with the use of cellular engineering in mammalian reproduction. The completion of the course will be helpful in his future professional work.

Entry requirements Animal reproduction, Developmental Biology, Fundamentals of biotechnology

Course contents

Lectures: The history, development and present status of the application of cellular engineering in mammalian reproduction. Reproductive potential of females. The methods for collection and storage of female gametes. Reproductive potential of males. The methods of obtaining the male gametes. Possibility of sperm uusing as a carrier of foreign genetic information in a transgenesis. Laboratory: The morphological assessment of oocytes from the ovary using histological slides. The obtaining of oocytes from the ovaries of some species, the assess the quality and usefulness of oocytes for research in vitro. The preparation of oocytes for in vitro maturation. The assessment of the oocytes maturity degree in IVM procedure. The evaluation of sperm, methods of sperm capacitation, the preparation of sperm for in vitro fertilization. The culture of embryos to the blastocyst stage. Evaluation of the embryos quality.

Assessment methods Grade, written exam


1. Hafez E.S.E., Hafez B.: Reproduction in farm animals. PhiladelphiA(U.A): Lippincott Williams & Wilkins, 2000.

2. Gordon I. R.: Reproductive technologies in farm animals. Wallingford, Oxfordshire, Cambridge, MA: CABI Pub., 2004.

Additional information Not more than 15 people in one student group.

Course title GENOMICS




[email protected]


ECTS points 5

Type of course Obligatory Level of course master

Semester Winter and summer Language of instruction English


12

Objectives of the course Get knowledge about genomes and methods of their analysis

Entry requirements Molecular biology and genetics

Course contents

Lectures. Introduction - history of genomics, fields, connection with other sciences. Size and structure of pro-, eukaryotic and organelle genomes with its comparison. Origin of new genes. Role of noncoding DNA. Sequencing of genes and genomes. Physical and genetic maps. Methods in functional genomics. Main genomic projects. Comparative genomics. Diseases caused by genome architecture. Laboratories. Organelle DNA isolation and analysis. Restriction mapping. Practical application of genomic databases.


Recommended readings 1. T. Brown “Genomes 3”, Garland Publishing , 2006 2. A.M. Lesk “Introduction to Genomics”, Oxford University Press, 2012 3. G. Gibson, S. Muse “A Primer of Genome Science, Third Edition”, Sinauer Associates Inc., 2009


Course title MOLECULAR BIOLOGY

Teaching method Lecture and laboratory


Arkadiusz Terman D.Sc. E-mail address to the person responsible for the course

[email protected]


ECTS points 5

Type of course Obligatory Level of course Bachelor/master


Hours per week 2 hours Hours per semester 30 hours

Objectives of the course Get knowledge on molecular mechanisms of gene expression, methods of molecular biotechnology

Entry requirements general genetics, molecular biology

Course contents

Molecular mechanisms of gene expression (transcription, translation), DNA repair, gene activity regulation, molecular biology of different traits. Molecular methods of nucleotide acids isolation, restriction enzymes analysis, DNA and RNA electrophoresis, primers digestion, preparation of molecular probes.

Assessment methods Written test, Presentation

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Recommended readings 1. L.A. Allison, Fundamental Molecular Biology, First Edition, Backwell Bublishing Ltd, Oxford 2007 2. T.A. Brown, Genomes 3, Garland Science Publishing, 2007


Course title GENETIC ENGINEERING METHODS

Teaching method Lecture and laboratory


Arkadiusz Terman D.Sc. E-mail address to the person responsible for the course

[email protected]


ECTS points 5

Type of course obligatory Level of course Bachelor/master

Semester Summer/winter Language of instruction English


Objectives of the course Get knowledge on molecular genetics methods of manipulation.

Entry requirements molecular biology methods

Course contents Assisted reproductive procedure, retrovirus vectors, transposons, Knock-in and Knock-out technology and also Marker – Assisted – Selection. Methods of animal manipulation, microinjection of DNA into the nucleus of anchored cells.

Assessment methods Written test, Presentation

Recommended readings 1. Russell, David W.; Sambrook, Joseph (2001). Molecular cloning: a laboratory manual. Cold 2. A.M. Lesk “Introduction to Genomics”, Oxford University Press, 2012


Course title MOLECULAR MODELING OF ENZYMES



Radoslaw Drozd, PhD E-mail address to the person responsible for the course

[email protected]

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ECTS points 5




Objectives of the course Get basic knowledge in the field of “insilico” methods for predictions and analysis the different levels molecular structure of enzymes

Entry requirements Basics of chemistry, biophysics, biochemistry, physical chemistry, informatics

Course contents Principles of enzymes molecular structure organization. Template based and free prediction of enzymes 3D structure. Methods for enzymes 3D structure modeling process verifications. In silico modeling catalytic properties of enzymes.

Assessment methods Project work


1. Janusz M. Bujnicki “Prediction of Protein Structures, Functions, and Interactions”. John Wiley & Sons, Ltd. 2008

2. Igor F. Tsigelny. Protein Structure Prediction: Bioinfomatic Approach. Internat'l University Line, 2002 – 493

3. Athel Cornish-Bowden “Fundamentals of enzyme kinetics” Portland Press, 2004 4. Jeffrey Cleland, Charles Craik. “Protein engineering: principles and practice” Wiley-Liss, 1996 5. Lilia Alberghina”Protein engineering in industrial biotechnology” Harwood Academic Publishers, 2000


Course title FOOD AND NUTRITION IN RELATION TO HUMAN HEALTH

Teaching method Lecture, seminar


Arkadiusz Pietruszka, D.Sc. E-mail address to the person responsible for the course

[email protected]


ECTS points 5

Type of course elective Level of course Bachelor/master

Semester winter/ summer Language of instruction English


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After the course accomplishment, students are aware of well-balanced diet on human health and resulting threats from improper nutrition. Students are knowledgeable about the basic knowledge in the field of dietetics, nutrigenomics and physiology of human nutrition. Students have an ability of the interactive view about the issue of the food, nutrition and health.

Entry requirements General knowledge within the scope of human physiology

Course contents

Current trends in human nutrition and nutritional prophylaxis. Health and its factors: genetic, environmental and associated with a lifestyle. Basic notions associated with well-balanced nutrition. Norms of human nutrition. Food pyramid. Nutrigenomics - understanding concepts at the food-genome junction. Alternative diets. Additions to the food and possibilities of applying alternative solutions. Biologically active substances in food products. Pollutions and threats on a plate.

Assessment methods

- Project work

- Continuous assessment

- Grade


1. Dansinger ML, Gleason JA, Griffith JL, Selker HP, Schaefer EJ: Comparison of the Atkins, Ornish, Weight Watchers, and Zone Diets for Weight Loss and Heart Disease Risk Reduction. The Journal of the American Association, 2005

2. Geissler C, Powers H: Human Nutrition. Churchill Livingstone Elsevier, 2011 3. Gibney MJ, Lanham-New SA, Cassidy A, Vorster HH: Introduction to Human Nutrition. A John Wiley &

Sons, Ltd., Publication, 2009 4. Kaixiong Y, Zhenglong G: Recent Advances in Understanding the Role of Nutrition in Human Genome

Evolution. Thematic Review Series: Nutrition and the Genome, 2011

Additional information Max. 15 persons per group

Course title MEDICAL AND VETERINARY MICROBIOLOGY



Karol Fijałkowski PhD E-mail address to the person responsible for the course

[email protected]


ECTS points 5




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Aims of the Course The course aims are to provide a comprehensive theoretical and practical knowledge of medical and veterinary microbiology and to provide students with the latest information in scientific microbiology. The student should be able to: Discuss the classification of bacteria; Describe the bacterial colony and cell structure; Explain the bacterial physiology and growth; Discuss the bacterial genetics; Describe in details the morphology, the culture, spread, biochemical activities, antigenic characters, pathogenesis, laboratory diagnosis; Define the organs commonly involved in the infection; Explain the methods of microorganisms controls.

Entry requirements Basic lab knowledge and skills. Ability to pipet, make solutions and dilutions and to execute protocols which require the use of sterile techniques.

Course contents

Information about working in microbiological laboratory; Methods for determination and controlling bacterial growth; Methods of culturing bacteria; Preparing and storage of culture media; Conditions of bacterial cultures; Microscopic examination of microorganisms; Procedure for preparing bacterial smears; Staining procedures; Detection and identification of various kind of microorganisms; Study of biochemical activity of microorganisms; Determination of antibiotic susceptibility of pathogenic bacteria; Identification of virulence factors produced by microorganisms; Molecular diagnostics of microorganisms – PCR based techniques; Isolation and identification of microorganisms from environmental samples and animal material and determination of their quantity; Isolations of pure bacteria from a mix culture; Validation of methods in microbiology laboratory.

Assessment methods Grade, project work, continuous assessment

Recommended readings 1. L. M. Prescott, “Microbiology”, McGraw-Hill Science, 2002. 2. C. L. Gyles, J. F. Prescott, J. G. Songer, C. O. Thoen “Pathogenesis of Bacterial Infections in Animals 4th Ed”, Blackwell Publishing, 2010.


Course title ENVIRONMENTAL TOXICOLOGY

Teaching method lecture



[email protected]


ECTS points 5

Type of course optional Level of course bachelor

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Objectives of the course The aim of the subject is to familiarize the students with an environmental contaminants and their effect on human and animal organisms

Entry requirements physiology, biochemistry

Course contents Major classes of pollutants, global transport, fate of metals, isotopes and other contaminants, metabolism of xenobiotics, biochemical and physiological effects of exposure to xenobiotics, factors influencing on xenobiotic’s toxicity.

Assessment methods test

Recommended readings 1. Principles of Ecotoxicology, CH Walker, SP Hopkin, RM Sibly, DB Peakall, CRC Taylor and Francis 3rd

Edition ISBN 084933635X


Course title IN VITRO AND IN VIVO METHODS IN TOXICOLOGICAL ASSESSMENT OF XENOBIOTICS

Teaching method lecture



[email protected]


ECTS points 5

Type of course optional Level of course bachelor



Objectives of the course The aim of the course is to familiarize students with alternative and convention methods used in toxicity assessment of xenobiotics

Entry requirements biochemistry

Course contents Database of in vitro techniques in the chemicals toxicity evaluation. Evaluation of chemical substances toxicity based on in vivo and in vitro tests. Relationship between the chemical structure and biological activity of xenobiotics. Evaluation of cosmetic products. Alternative methods in ecotoxicological studies.

Assessment methods test

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Recommended readings 1. Curtis Klaassen, 2013. Casarett & Doull's Toxicology: The Basic Science of Poisons, Eighth Edition.

ISBN-13: 978-0071769235


Course title CELLULAR ENGINEERING IN ANIMAL REPRODUCTION







ECTS points 5





Student knows the most important facts and achievements in the field of cellular engineering in mammalian reproduction. He knows the factors that determine the reproductive potential of mammals. Student specifies and describes methods of cellular engineering used in manipulations on gametes and embryos. Student knows methods of embryos collection and transfer. He describes potential benefits of embryo transfer in animal husbandry. Students know how to acquire and assess the quality of gametes. He can carried out sperm capacitation and set up the cultures in procedures IVM, IVF and IVC. Students knows how to evaluate the quality of embryos. He can correctly schedule of embryo transfer procedure. Student is aware of the importance of the knowledge. He knows the advantages and limitations associated with the use of cellular engineering in mammalian reproduction. The completion of the course will be helpful in his future professional work.

Entry requirements Animal reproduction, Developmental Biology, Fundamentals of biotechnology

Course contents

Lectures: The history, development and present status of the application of cellular engineering in mammalian reproduction. Reproductive potential of females. The methods for collection and storage of female gametes. Reproductive potential of males. The methods of obtaining the male gametes. Possibility of sperm using as a carrier of foreign genetic information in a transgenesis. Laboratory: The morphological assessment of oocytes from the ovary using histological slides. The obtaining of oocytes from the ovaries of some species, the assess the quality and usefulness of oocytes for research in vitro. The preparation of oocytes for in vitro maturation. The assessment of the oocytes maturity degree in IVM procedure. The evaluation of sperm, methods of sperm capacitation, the preparation of sperm for in vitro fertilization. The culture of embryos to the blastocyst stage. Evaluation of the embryos quality.



1. Hafez E.S.E., Hafez B.: Reproduction in farm animals. PhiladelphiA(U.A): Lippincott Williams & Wilkins, 2000.




19


Course title METHODS OF MONITORING THE REPRODUCTIVE PROCESSES IN ANIMALS

Teaching method Lecture/auditorium






ECTS points 4





Student knows the methods of monitoring ovarian cycle, taking into account the various phases of the cycle and its possible disorders. Student knows the basics of hormonal methods in the detection of pregnancy. Student knows the modern methods used in the andrological diagnostics. Student should be able to apply the correct method for monitoring ovarian cycle and pregnancy. Student should also interpret the parameters to indicating of reproduction disorders. Student will be able to apply the knowledge and skills to the proper selection and interpretation of the results in the evaluation of reproductive processes. The completion of the course will be aid in the laboratory work in the veterinary and medicine.

Entry requirements Knowledge of biotechnology in animal reproduction

Course contents

Lectures: Monitoring the ovarian cycle. Diagnostic methods of ovarian dysfunction (ovarian cysts, ovarian tumors). Hormonal basis for the detection of pregnancy. Advancements in imaging of the pregnancy. Modern diagnostics in andrology. Auditorium: Indirect methods for monitoring ovarian cycle (oestrus symptoms, hormone tests, evaluation of the cervical mucus and its degree of crystallization, the assessment of cytological smears, body temperature). Direct methods for monitoring ovarian cycle (laparoscopy, ultrasound). Methods of pregnancy diagnosis (detection of pregnancy, pregnancy monitoring). The monitoring of seasonal reproductive processes. Macroscopic evaluation of ovarian cysts and abnormalities in the reproductive organs.

Assessment methods Grade


1. Hafez E.S.E., Hafez B.: Reproduction in farm animals. PhiladelphiA (U.A): Lippincott Williams & Wilkins, 2000.


3. Ball P.J.H., Peters A.R. Reproduction in Cattle. Third edition. Blackwell Publishing, 20014




20

Course title ANIMAL EMBRYLOGY







ECTS points 4





Student defines the basic terminology in the field of embryology. Student describes the various stages and mechanisms of embryonic and fetal development. Student knows the course of implantation. Student describes the function of the auxiliary organs and shows the importance of fetal-placental endocrine system in the developing fetus. He knows the mechanism of parturition. Student is able to determine the degree of development of the embryo end fetus on the basic of the morphotic characteristic. Student is able to assess the placenta and fetal membranes of individual species. Student points to differences in fetal blood morphological image. After completing the course student will have a basis for the study of scientific disciplines related to the obtaining embryos in vitro, cloning, transgenesis and transplantation.

Entry requirements Knowledge of animal physiology and biotechnology in animal reproduction

Course contents

Lectures: Embryology as a scientific discipline and a range of modern of animals embryology. The course and types of implantation. The role of fetal-placental endocrine system in the developing fetus. Hormonal regulation of pregnancy and parturition. The development and metabolism of the embryo in the initial post-implantation period. The mechanism of twin pregnancy. Adapting to embryo/foetal life and the role of transitional organs. Mechanisms of organogenesis and chronological division of differentiation the final organs. Differentiation of mesodermal organs. Embryonic induction. The possibility of using cord blood in transplantology. Laboratory: Types of placenta for a specific species of mammals and anatomical differences in their construction. Structure and function the fetal membranes. Pregnancy (calculation date of birth). Parturition. Fetal development and maturity of the fetus. The blood circulation in placenta. Hematopoietic development: the development of erythrocytes and white blood cells. Age assessment embryo and fetus based on the size and formed the contours of the body.



1. Hyttel P., Sinowatz F., Vejlsted M., Betteridge K.: Essentials of Domestic Animal Embryology (E-Book), Elsevier 2010.

2. Hafez E.S.E., Hafez B.: Reproduction in farm animals. PhiladelphiA (U.A): Lippincott Williams & Wilkins, 2000.




21

Course title GENETIC MARKERS FOR FOOD QUALITY

Teaching method Lectures, classes



[email protected]


ECTS points 5

Type of course Optional Level of course Bachelor/master



Objectives of the course To familiarize students with possibility of genetic markers use in food analysis. Practical use of DNA analysis to assess food quality.

Entry requirements Basics of Genetics, Physiology and Molecular Genetics

Course contents

Introduction, history, basic terms, markers classes, criteria of markers application. Genetic markers of taste and food preferences. Genetic markers of food allergies. Genetic diagnosis of foodborn pathogens. Methods for GMO detection. Application of markers in food authentication. Genetic markers for:

fruit and vegetables quality;

milk quality and quantity;

different meat species quality. Application of genetic markes in nutrigenomics. Barcoding application in food analysis. Methodological approach for food markers detection.


Recommended readings 1. Da-Wen Sun “Modern Techniques for Food Authentication” Academic Press, 2008 2. Stefano Sforza “Food Authentication Using Bioorganic Molecules” DEStech Publications, Inc., 2013 3. Ioannis Sotirios Arvanitoyannis “Authenticity of Foods of Animal Origin” CRC Press, 2015


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