faster analyze more samples while maintaining resolution. web.pdf · faster run times, such as...

NEW Agilent J&W High Efficiency Capillary GC Columns

Analyze more samples

while maintaining resolution.faster

“We need to run twice as many

samples in the same amount of

time with the same resources.”

“I want to speed up my sample

run time without sacrificing

performance.”

“We have to find a way to reduce

our cost per analysis without

expensive system modifications

and time-consuming method

development.”

Now it is possible to achieve high efficiency,high throughput, and high resolution…without the high costs.Whether you are determining the purity of aromatic hydrocarbons… analyzing residualsolvents in drug substances… or screening for organic contaminants, chances are goodthat you’re under pressure to increase productivity while your resources remain constant.

That is why we’re so enthusiastic about our newest GC columns – one that sets a newstandard for speed and accuracy.

Introducing Agilent J&W High Efficiency Capillary GC Columns: a faster, more reliable way to run high-throughput analysis.

Agilent J&W High Efficiency Capillary GC Columns are ideal for applications that requirefaster run times, such as high-throughput screening, fast process monitoring, and fastmethod development. They are also flexible enough to be used in a wide variety ofenvironmental, petrochemical, flavor/fragrance, clinical toxicology, and pharmaceuticalsample matrices.

Decrease your run time by 50% or more, compared to conventional GC.

Agilent J&W High Efficiency Capillary GC Columns can reduce your sample run timeby 50% or more without compromising your resolution. So you can improve yourproductivity and meet the tightest time constraints.

Get the reliable results you need using the resources you have.

Unlike competitive 0.1 mm ID columns, Agilent J&W High Efficiency Capillary GC Columnsare compatible with all standard-pressure capillary GC and GC/MS instruments withoutexpensive high-pressure modifications. They also give you:

• The flexibility to choose between helium and hydrogen carrier gases. You canstay with a helium carrier if you wish to simplify method development, or switch to ahydrogen carrier if faster analysis is desired.

• The ability to separate samples using less carrier gas, which can lead to longerintervals between cylinder changes, increased uptime, and a lower cost per sample.

Proven reliability and robustness in real-life sample matrices.

A study conducted by the United States Environmental Protection Agency confirmed that, under actuallaboratory conditions:

Twice as many samples were analyzed over a 9-month period using Agilent 0.18 mm High EfficiencyCapillary Columns, compared with 0.32 mm I.D. columns.

Agilent 0.18 mm High Efficiency Capillary Columns showed remarkable durability, and lastedapproximately as long as 0.32 mm I.D. columns.

Source: The Journal of Chromatographic Science: September 2002.

2

3

A Direct Column-PerformanceComparison for Rapid CLP (ContractLaboratory Program) Pesticide Analysis

As you can see, Agilent’s DB-17msprimary analysis column resolved all 22peaks of interest in less than 6 minutes withsharp symmetry and minimal baseline drift.

Conversely, vendor R’s primary analysiscolumn resolved only 20 of 22 peaks – and displayed evidence of peak tailing.

Agilent’s DB-XLB confirmatory analysis column resolved 20 peaks of interest in less than 6 minutes (theremaining peaks were close to beingbaseline resolved and sufficient for peakconfirmation.).

Vendor R’s confirmatory column resolved all22 peaks of interest; however there isevidence of peak tailing, as well as anunacceptable level of temperature-dependent baseline drift. Compare that to Agilent’s results, which show sharp,symmetrical peaks and minimal temperature-dependent baseline drift.

When it comes to speed and resolution, Agilent HighEfficiency GC Columns outperform a leading competitor.And this side-by-side comparison proves it!

min1 2 3 4 5 6

Hz

100

125

150

175

200

225

250

275

300

min1 2 3 4 5 6

Hz

150

200

250

300

350

400

1

23

4

56

7 8

9

1011

12 13

1415

1617

18

19

20

21

22

DB-17ms primary columnAgilent part #121-4722

DB-XLB confirmatory columnAgilent part #121-1222

12

3

4

56 7 89

1011

1213

1415

16

1718

19

20

21

22

min2 3 4 5 6

Hz

100

150

200

250

300

350

400

450

Co-elution

Vendor R primary column

min1 2 3 4 5 6

Hz

200

300

400

500

600

700

Baseline rise

Peak tailing

1

Vendor R confirmatory column

{

Peak tailing

To start achieving faster separations – and results you can trust –go to www.agilent.com/chem/HEcolumns2

Columns: DB-17 ms (20 m x 0.18 mm x 0.18 µm)DB-XLB (20 m x 0.18 mm x 0.18 µm)

Carrier: Hydrogen (69 cm/sec at 120% C, ramped at 99 ml/min to106 cm/sec at 4.4 minutes)

Inlet: Split/splitless; 220% C, pulsed splitless (35 psi for 0.5 min,purge flow of 40 ml/min on at 1 minute, gas saver flow 20 ml/min on 3 minutes)

Oven: 120% C (0.32 min); 120 % C/min to 160% C; 30 % C/min to258%C (0.18 min); 38.81% C/min to 300% C (1.5 min)

Detector: µECD 320% C; nitrogen makeup; constant column + makeupflow 60 ml/min

1. Tetrachloro-m-xylene2. Alpha BHC3. Gamma BHC4. Beta BHC5. Delta BHC6. Heptachlor7. Aldrin8. Heptachlor Epoxide9. Gamma Chlordane10. Alpha Chlordane11. Endosulfan I12. 4,4’ DDE 13. Dieldrin14. Endrin15. 4,4’ DDD16. Endosulfan II17. 4,4’ DDT18. Endrin Aldehyde19. Endosulfan Sulfate20. Methoxychlor21. Endrin Ketone22. Decachlorobiphenyl

Part #121-4722

Part #121-1222

To learn more, reference publication number 5989-8031EN at www.agilent.com/chem

44

0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00

2000000

4000000

6000000

8000000

1e+07

1.2e+07

1.4e+07

1.6e+07

1.8e+07

2e+07

2.2e+07

2.4e+07

2.6e+07

2.8e+07

3e+07

3.2e+07

3.4e+07

3.6e+07

2 2

2 1

2 0

1 9

1 8

1 7

1 6

1 5

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1 3

1 2

1 11 0

7

5 ,6

4

3

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1 6

1 51 4

1 3

1 2

1 11 0

98

7

5 ,6

4

3

2

1

2 00 4.00 6.00 0 10. 0 12.00 14.00 16.0 0 18.00

1 e+ 0 7

2 e+ 0 7

3 e+ 0 7

4 e+ 0 7

5 e+ 0 7

6 e+ 0 7

7 e+ 0 7

8 e+ 0 7

9 e+ 0 7

1 e+ 0 8

1 . 1 e+ 0 8

1 . 2 e+ 0 8

1 . 3 e+ 0 8

1 . 4 e+ 0 8

1 . 5 e+ 0 8

1 . 6 e+ 0 8

1 . 7 e+ 0 8

1 . 8 e+ 0 8

1 . 9 e+ 0 8

T

p ons e

2 2

2 1

2 0

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1 7

1 6

1 5

1 4

1 3

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1 1

1 0

9

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7

5 ,6

4

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1

.0 8.0 0 .0

1 e+ 0 7

2 e+ 0 7

3 e+ 0 7

4 e+ 0 7

5 e+ 0 7

6 e+ 0 7

7 e+ 0 7

8 e+ 0 7

9 e+ 0 7

1 e+ 0 8

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1 . 3 e+ 0 8

1 . 4 e+ 0 8

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1 . 8 e+ 0 8

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T im e

R e sp ons e

G C 3 - 6 707.D \E C D 2 B

2 2

2 1

2 0

1 9

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1 0

9

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4

3

2

1

Contract Laboratory Program (CLP) pesticide analysis on High Efficiency (0.18 mm I.D.)GC columns. In this example, the analysis of 22 CLP pesticides were achieved in 16minutes using the original method, whereas the improved method was completed in just under 7 minutes. That’s a 56% faster sample run time.

Original Method (using a 0.32 mm I.D. column

and Helium carrier)

"Faster" Method (using a High Efficiency

GC Column and H2 carrier)

Column: DB-XLB20 m x 0.18 mm i.d., 0.18 µm

Carrier: H2, constant flow, 77.3 cm/s at 120 °CInjector: Pulsed Splitless, 220 °C

Pulse pressure & time: 35psi for 0.5 min Flowramp at 6.25 min of 99 mL/min 2 to 3 mL/min2 mm i.d. liner0.5 µL, 50 ppb

Oven: 120 °C for 0.49 min120 °C to 160 °C at 59.4 °/min160 °C to 260 °C at 23.7 °/min260 °C to 300 °C (1.69min) at 35.6 °/min

Detector: µ-ECD, 320 °CAr/CH4 (P5) makeup gas at 60mL/min

Column: DB-XLB30 m x 0.32 mm i.d., 0.25 µm

Carrier: He, constant flow, 38 cm/s at 120 ºCInjector: Pulsed Splitless, 220 ºC

Pulse pressure & time: 35 psi for 1.15 min2 µl, 50 ppb

Oven: 120 ºC for 1.17 min120 ºC to 160 ºC at 25 º/min160 ºC to 260 ºC at 10 º/min260 ºC to 300 ºC (4 min) at 15 º/min

Detector: µ-ECD, 320 ºCAr/CH4 (P5) makeup gas at 60 mL/min

1. TCMX2. Alpha BHC3. Gamma BHC4. Beta BHC5. Delta BHC6. Heptachlor7. Aldrin8. Heptachlor Epoxide9. Gamma Chlordane10. Alpha Chlordane11. Endosulfan I12. 4,4’ DDE13. Dieldrin14. Endrin15. 4,4’ DDD16. Endosulfan II17. 4,4’ DDT18. Endrin Aldehyde19. Endosulfan Sulfate20. Methoxychlor21. Endrin Ketone22. DCB

Environmental and Food Safety

Here are some

industry-specific

examples of how

Agilent J&W High

Efficiency Capillary

GC Columns can

open the door to

fast GC applications.

Part #123-1232

Part #121-1222


5

Chemical and Petrochemical Industry

10.6 min

17.7 min

27.4 min

(0.25 mm, He Carrier)

D -16.8 min (0.18 mm, H2 Carrier)

D -9.7 min (0.18 mm, He Carrier)

Spearmint Oil Spearmint Oil – Resolution Check

Foods, Flavors, and FragrancesThese applications demonstrate how changes to column dimension (both alone and in combination with carrier gas modifications) affect sample run time.Agilent’s Method Translation Software was used to scale existing 0.25 mm I.D. column methods to 0.18 mm I.D. columns. Note that, in addition to thesignificant gains in speed, resolution was well maintained throughout the method translation process.

D-9.7 min (0.18 mm, He Carrier)

10.6 min

17.7 min

3 8 13 18 23 28


Time (min)

27.4 min



10.6 min

17.7 min

3 8 13 18 23 28


Time (min)

27.4 min



10.6 min

17.7 min

3 8 13 18 23 28


Time (min)

27.4 min


1 Heptane2 Cyclohexane3 Octane4 Nonane

5 Benzene6 Decane7 Toluene8 1,4-Dioxan

9 Undecane10 Ethylbenzene11 p-Xylene 12 m-Xylene

13 Cumene14 Dodecane15 o-Xylene16 Propylbenzene

17 p-Ethyltoluene18 m-Ethyltoluene19 t-Butylbenzene20 s-Butylbenzene

21 Styrene22 Tridecane23 1,3-Diethylbenzene24 1,2-Diethylbenzene

25 n-Butylbenzene26 a-Methylstyrene27 Phenylacetylene

min0 1 2 3 4 5 6

pA

0

50

100

150

200

250

300

350

400

1

2 34

5

67

8

910 11

12 13 14 1516

17

1819

20

2122

2324

25

26

27

min2.5 5 7.5 10 12.5 15 17.5 20 22.5

pA

0

50

100

150

200

1

2

34

56

7

8

9 101112 1314 1516

1718

19

20

21 22

23 2425

26

27

This application showcases the practicality using high efficiency GC columns in daily aromatic solvent analysis. The result: a three-fold reduction in run time (compared to a 0.32 mm I.D. column) with no change in resolution.

Unified aromatic solvent method with a 60 m x 0.32 mm x 0.5 mm HP-INNOWax column

Optimized unified aromatic solvent method with a 20 m x 0.18 mm x 0.18 mm HP-INNOWax column

To order, go to www.agilent.com/chem/HEcolumns2

Part #19091N-216

DB-1, 20 m x 0.18 mm x 0.18 µm Part #121-1022

DB-1, 20 m x 0.18 mm x 0.18 µm Part #121-1022

DB-1, 30 m x 0.25 mm x 0.25 µm Part #122-1032

Part #19091N-577



Eliminate time-consuming method re-development with Agilent’s FREEMethod Translation Software.

Method Translation Software is a fast, reliable tool for translating methods to Agilent’s 0.18 mmcolumn dimension. This software creates a perfectly scaled version of your original method, whileallowing you to rapidly predict required gas velocity, oven temperature program rates, and relativerun times.

You should use Method Translation Software whenever you change carrier gas type, columndimensions, phase ratio, column head pressure, carrier gas flow rate, or holdup time; however, theone assumption is that you do not change your stationary phase.

To download Agilent’s Method Translation Software, go to www.agilent.com/chem/myGCcolumns

With 65 part numbers and 23 stationary phases, Agilent has the largestselection of High Efficiency GC Columns for the widest range of applications.

DB-1

HP-1

DB-1ms

HP-1ms

DB-5

HP-5

DB-5ms

HP-5ms

DB-XLB

6

StationaryPhase

Part Number(7” cage)

I.D. (mm)

Length (m)

Film Thick-ness (µm)


StationaryPhase


I.D. (mm)

Length (m)

Film Thick-ness (µm)


121-1012

121-1013

121-101A

121-1022

121-1023

121-1043

19091Z-577

121-0122

19091S-677

121-5012

121-5013

121-5022

121-5023

121-5042

19091J-577

121-5522

121-5542

121-5523

19091S-577

121-1222

121-1232

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

10

10

10

20

20

40

20

20

20

10

10

20

20

40

20

20

40

20

20

20

30

0.18

0.40

0.20

0.18

0.40

0.40

0.18

0.18

0.18

0.18

0.40

0.18

0.40

0.18

0.18

0.18

0.18

0.36

0.18

0.18

0.18

121-1012E

121-1013E

121-1022E

121-1043E

19091Z-577E

121-0122E

19091S-677E

121-5012E

121-5022E

121-5023E

19091J-577E

121-5522E

19091S-577E

121-1222E

DB-35ms

DB-17

DB-17ms

HP-50+

DB-23

DB-225

DB-624

DB-1301

DB-1701

DB-WAX

HP-INNOWax

DB-5.625

DB-VRX

DB-608

121-3822

121-1722

121-1723

121-4722

19091L-577

121-2323

121-2223

121-1324

121-1313

121-0713

121-0722

121-7012

121-7022

121-7023

121-7042

121-7043

19091N-577

121-5621

121-5622

121-1524

121-1544

121-6822

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

0.18

20

20

20

20

20

20

20

20

10

10

20

10

20

20

40

40

20

20

20

20

40

20

0.18

0.18

0.30

0.18

0.18

0.20

0.20

1.00

0.40

0.40

0.18

0.18

0.18

0.30

0.18

0.30

0.18

0.18

0.36

1.00

1.00

0.18

121-3822E

121-4722E

121-1224E

121-7022E

121-7023E

121-7042E

19091N-577E

121-1544E

7

Agilent J&W High Efficiency Capillary GCColumns are part of your total solution forfast GC. Agilent J&W High Efficiency Capillary GC Columns are compatible with Agilent 5890,6890, 6850, and 7890 GC systems and detectors – including our 7890A GC. What’smore, the backflush and cooling capabilities of Agilent’s 7890A GC can further increaseyour sample throughput.

Keep in mind, too, that Agilent splitless liners, gold seals, QuickSwaprestrictors, and fittings are engineered to work with your Agilent GC or GC/MSsystem. They are manufactured to our exact specifications to make sure your systemperforms to its full potential – while preventing contamination and leaks that can lead to costly downtime and jeopardize the integrity of your results.

To learn how Agilent's 7890A GC, together withour High Efficiency GC Columns, can take yourresults to the next level, go towww.agilent.com/chem/7890A

When you buy instruments, columns, and suppliesfrom Agilent, you get more than just reliability.

You also get:

• Over 40 years of chromatography expertise.

• Unmatched technical support – on the Web, by phone or in person.

• A 90-day warranty from the date of shipment.

To learn more about Agilent J&W High Efficiency Capillary GC Columns – or Agilent products and services – visit us online or call toll free:

www.agilent.com/chem/HEcolumns2

1-800-227-9770, option 3, then option 3 again (in the US and Canada)

In other countries, please call your local Agilent Representative or Agilent Authorized Distributor.

Information, descriptions, and specifications in this

publication are subject to change without notice.

© Agilent Technologies, Inc. 2008

Printed in the U.S.A. April 8, 2008

5989-8022EN

Agilent now offers capillary GC columns

designed for fast analysis using

comprehensive gas chromatography

(GC x GC). GC x GC utilizes two capillary

columns in series, and is a powerful tool

for analyzing complex mixtures such

as petrochemicals, fragrances

and environmental samples.

faster analyze more samples while maintaining resolution. web.pdf · faster run times, such as...

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