f.d.ovcharenko insitute of biocolloidal chemistry national academy of sciences of ukraine kyiv...
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F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY
NATIONAL ACADEMY OF SCIENCES OF UKRAINENATIONAL ACADEMY OF SCIENCES OF UKRAINE
KyivKyiv
GOLD-BEARING PROBIOTIC DRUG GOLD-BEARING PROBIOTIC DRUG “OKARIN-“OKARIN-AUAU””
Valeriya VEMBERValeriya VEMBER
Telephone: +380-44-4241919
Workshop “From Science to Business”
11-12 October, 2006
Kyiv
Proprietary information statementProprietary information statement
• The technology material presented in this talk is available for licensing and joint product development.
• None of the slides contain any confidential or proprietary information.
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The shape of gold-bearing probiotic The shape of gold-bearing probiotic preparationpreparation " "Okarin-AuOkarin-Au""
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increase of stressful factors quantity and strength
deterioration of ecological situation
uncontrolled usage of antibiotics and other medicines
using great amount of junk food
GastrointestinalGastrointestinal tract disorderstract disorders
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B B ii oo tt hh ee rr aa pp yy
Probiotic food
PrebioticProbiotic
substances, which selectively stimulate
the growth and biological activity of microorganisms in intestines, which
positively influence on the
microbiocenosis structure
live microorganisms;
metabolites produced by some microorganisms to stimulate the growthof other ones, etc.
foodstuff bearing by probiotics
Main problems as to probiotic drugs’ Main problems as to probiotic drugs’ development and application development and application ::
limited antagonistic activity of the strains-producers to the numerous pathogenic agents;
insufficient growth speed of biomass producing strains;
high cost of probiotic preparation as a result of using the expensive growth substances for strains-producers growing;
non-observance of the microecological adequacy principle while treatment of different dysbacteriosis types have been provided
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Bringing the appropriate metals into the organism gives good therapeutic results in case of illnesses, which are characterised by
considerable changes in metal-microelement balance
ProbioticProbiotic + + PrebioticPrebiotic = = SynbioticSynbiotic
M E T A L SM E T A L S── important regulatory environmental compounds andimportant regulatory environmental compounds and
potential ingredient for potential ingredient for SynbioticsSynbiotics
ADVISABILITYADVISABILITY of P of PROBIOTICROBIOTIC DRUG DRUG
ENRICHMENTENRICHMENT by by COLLOIDALCOLLOIDAL Au and Ag Au and Ag
Investigation of metals influence on some nonspecific physiological and biochemical parameters,
which have been of great importance for all the metabolic processes that take place in bacterial cell :
Growth processes’ intensity
Cell’s wall permeability
ATP-ase activity Energy function of bacterial plasma membrane
Level of lipid peroxidation Antioxidant enzymes activity
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Changes of biological activity of bacterial Changes of biological activity of bacterial association “association “OkarinOkarin” after incubation with GOLD ” after incubation with GOLD
(10(10-6-6 mkgmkg//mlml) ) and SILVERand SILVER (10 (10-7-7 mkg/mlmkg/ml))
Metals Form
Investigated indexes
Growth intensity
ATP-ase activity
H+ -
extrusion
Perme-ability of the cell
wall
Level of lipid peroxi-
dation
Activity of antioxidant enzymes
Catalase SOD
AuColloidal 0
Ion 0 — — 0 0 0
AgColloidal 0 — — 0 0
Ion — —
Kind of changes : – positive; – negative; «—» – investigation is not conducted. 88
RResistance esistance of of “Okarin”“Okarin” and and“Okarin-Au” “Okarin-Au” to to the the antibioticantibioticss
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Antagonistic activity of Antagonistic activity of bacterial association bacterial association ““OkarinOkarin””
and and gold-bearing gold-bearing ““Okarin-AuOkarin-Au””
Microorganisms(test object)
Size of lysis zone after 24 hours,
mm
Quantity of test-culture (pieces) (Х ± m)
ControlControl after 12 hoursafter 12 hours after 24 after 24 hourshours
after 72 after 72 hourshours
OkarinOkarin-
AuOkarin
Okarin- Au
OkarinOkarin-
AuOkarin
Okarin- Au
OkarinOkarin-
Au
Shigella flexneri 1а 20 — 159 ± 9 — 128 ± 14 — 84 ± 7 — 12 ± 2 —
Sh. flexneri 1в 20 — 132 ± 12 — 111 ± 8 — 58 ± 7 — 14 ± 5 —
Sh. flexneri 2а 20 20 107 ± 16 107 16 92 ± 4 96 7 59 ± 6 64 8 27 ± 8 9 1
Sh. flexneri VI 20 20 183 ± 5 183 5 131 ± 11 101 10 81 ± 10 47 4
18 ± 3 7 3
Sh. sonnei 20 20 120 ± 7 120 7 92 ± 3 89 6 76 ± 11 51 6
25 ± 4 18 1
Salmonella enteritidis 20 20 100 ± 14 100 14 86 ± 7 78 3 51 ± 2 47 7 29 ± 8 14 3
S. thyphimurium 15 20 127 ± 16 127 16 120 ± 7 85 5
78 ± 12 68 2 25 ± 7 16 5
Staphylococcus aureus 15 20 116 ± 6 116 6 109 ± 8 73 4
59 ± 11 60 10 24 ± 10 24 5
Pseudomonas aeruginosa 15 20 110 ± 2 110 2 104 ± 9 8111
79 ± 3 64 9
36 ± 8 31 2
Ps. vulgaris Not find — 122 ± 7 — 105 ± 2 — 85 ± 7 — 34 ± 9 —
* ─ behaviors, which show the improvement of initial preparation “Okarin” by colloidal gold. 1010
Wide range of antagonistic activity to pathogenic microorganisms causing dysbacteriosis and especially dangerous infection diseases including anthrax, rabbit-fever, salmonellosis, brucellosis etc. (according to results of clinical trials);
Selective bactericidal activity to pathogenic microflora and simultaneous stimulation of normoflora. This feature is intensified owing to the presence of colloidal gold particles;
Low cost of the treatment course in a comparison with other probiotic drugs which are available on the market today;
Ability to increase the lysis of atypical pathological cells treatment application opportunity for oncological patients due to high resistance of probiotic's microbial cultures to antibiotics and chematherapeutic agents;
Non-specific immune-resistance stimulation ability of the whole organism;
Suitable producing shape and application simplicity.
Innovative Aspect Innovative Aspect and Main Advantagesand Main Advantages
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Clinical trials’ results ofClinical trials’ results ofbacteriological preparation "bacteriological preparation "Okarin-AuOkarin-Au""
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PathogenPathogenousous species species::Staphylococcus aureus, Enterobacter aerogenes, Klebsiella pneumoniae,Candida albicans
0
15
30
45
Th
e n
um
ber
of
mic
roor
gan
ism
s,
lg
C
Normal flora Normal flora of GIT:Bifidobacterium, Lactobacillus, Escherichia coli
DysbiosisDysbiosisState
NORMNORMALAL State
Standard PGT + Okarin-AuOkarin-Au
Standard PG TherapyTherapy
Competitive Matrix of ProbioticCompetitive Matrix of Probiotic Probiotic’s Probiotic’s
Name Name
Probiotic’sProbiotic’s
CharacteristicCharacteristic
Okarin-Au
(IBCC-UkrInkom, Ukraine)
Colibac-terinum
(Biofarma, Ukraine)
Hylak Forte
(Ratiopharm, Germany)
Linex
(LekPharma, Slovakia)
Bifiform
(Ferrosan, Denmark)
Preparation Composition
E. coli (3 strains) + Enterococcus faecalis (dried alive cultures, enriched by colloidal Gold) – 1:1:1:3.
Capsules.
E. coli (strain M-17) (dried alive cultures) –
1*1010 CFU/dose. Ampoules.
Metabolites of E. coli, Streptococcus faecalis,
Lactobacillus acidophilus and L. helveticus +
growth factors (aqueous solution) – 2:1:1:4.
Bottles.
Lactobacillus acidophilus,
Bifidobacterium infantis, Enterococcus faecium (dried alive cultures) –
1,2*107 CFU/dose (each of strains). Capsules.
Bifidobacterium longum and
Enterococcus faecium (dried alive
cultures) – 1*107
CFU/dose (each of strains). Capsules.
Antagonistic activity (AA)
Shigella flexneri, Sh. sonnei, Salmonella enteritidis, S. thyphimurium, Staphylococ-cus aureus, Pseudomonas aeruginosa, Ps. vulgaris, Enterobacter aerogenes, Klebsiela pneumonia, Candida albicans
Mixed infection of GIT and
Salmonellosis.
Prohibited to use in the case of
Escherichiosis !!!
Mixed infection of GIT and
Salmonellosis
Wide range
of AA (accordance to
preparation Instruction)
Wide range
of AA
(accordance to preparation Instruction
Dosage regimen (per 24 hours)
2-3 capsules 6-12 doses 120-180 drops 6 capsules 2-4 capsules
Cost of treatment
for 10 days$ 10 $ 7 $ 11 $ 15 $ 12,5
Complications and peculiarities of
application―
1) Require preliminary preparation;
2) Prohibited to use at the same time with Antibiotics
1) Require preliminary measuring;
2) Prohibited to use at the same time with Milk and Dairy Produce
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• We seek for potential partners interested in new type of combined (and effective!) probiotic preparation and partners, who can help us in putting them on the market
• We are ready for collaboration with the purpose of creation of different synbiotic drugs which include wide range of colloidal metals and have different therapeutic effects
O p p o r t u n i t i e sO p p o r t u n i t i e s
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Contact Information :Contact Information :
Prof. Zoya ULBERGTelephone: +380 – 44 – 424 80 78
e-mail: [email protected]
F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY
NATIONAL ACADEMY OF SCIENCES OF UKRAINENATIONAL ACADEMY OF SCIENCES OF UKRAINE
KyivKyiv
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