feedlot performance, net energy and carcass merit …

82
FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT AS AFFECTED BY HIGH MOISTURE VS. DRY METHODS OF PROCESSING MILO By Dennis Ralph White Bachelor of Science Oklahoma State University Stillwater, Oklahoma 1964 Submitted to the Faculty of the Graduate College of the Oklahoma State University in partial fulfillment of the requirements for the Degree of MASTER OF SCIENCE August, 1969

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Page 1: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

FEEDLOT PERFORMANCE, NET ENERGY AND

CARCASS MERIT AS AFFECTED BY HIGH

MOISTURE VS. DRY METHODS

OF PROCESSING MILO

By

Dennis Ralph White

Bachelor of Science

Oklahoma State University

Stillwater, Oklahoma

1964

Submitted to the Faculty of the Graduate College of the

Oklahoma State University in partial fulfillment of

the requirements for the Degree of

MASTER OF SCIENCE August, 1969

Page 2: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

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Page 3: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

j

OKLAHOMA STATE UNIVERSllY I..IBRARY

NOV 5 1969

~ .... ;..\~ ·r. ,.\.--":• _..f, ~••}., , , ·; , w·,,. •• ·~· ~-. ,;,-.r

FEEDLOT PERFORMANCE 0 NET ENERGY AND

CARCASS MERIT AS AFFECTED BY HIGH

MOISTURE VS. DRY METHODS

OF PROCESSING MILO

Thesis Approveds

730172

ii

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ACKNOWLEDGEMENTS

The author expresses his sincere appreciation to

Dr. Robert Totusek, Professor of Animal Science, for his

counsel and guidance during the course of this study and in

the preparation of this thesiso

Acknowledgement is due Dro lo To Omtvedt 9 Associate

Professor of Animal Science and Dr. Donald Go Wagner, Assis­

tant Professor of Animal Science, for their assistance in

the preparation of this thesis and to John Collier for his

assistance in analysis of datao

Appreciation is extended to Vincent Neuhaus, Jim Newsom,

and Bill Schneider 0 Graduate Assistants in Animal Science;

Dwight Stephens 0 Superintendent 9 Robert Renbarger, Assistant

Superintendent 0 and other personnel at the Fort Reno Experi=

ment Station, in cooperation with the United States Depart~

ment of Agriculture 0 Agricultural Research Service 0 Animal

Husbandry Research Division~ and to student workers at

Oklahoma State University for their assistance in conducting

this studya

The author is also grateful to fellow graduate students

at Oklahoma State University for their help and suggestions.

Acknowledgement is extended to Farmland Industries for

financial support of the research reported hereino

iii

Page 5: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

Special acknowledgement is given to the author's wife,

Norma, for her encouragement and assistance throughout the

course of this study and for typing the manuscript,

iv

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TABLE OF CONTENTS

Chapter Page

I. INTRODUCTION • • • 0 • • • • • • 0 0 • 0 0 0 0 • l

II. REVIEW OF LITERATURE • • • • • • 0 • • • 0 • • 2

Methods of Processing 0 • • • • 0 0 • • • 0 2 Grinding .. • • • •· • • • • 0 0 • • • • 3 Dry Rolling. • 0 • • • • • • • • • • • 4 Pelleting. • 0 0 0 • • • • • 0 0 0 0 • 6 Steam Flaking, • • • • • 0 0 0 0 0 0 0 8 High Moisture Processing • 0 • 0 0 • • 12

III. MATERIALS AND METHODS. 0 0 • • • • • 0 • 0 • • • 16

General • • • • • 0 • 0 • • • • • • • ·• 0 0 16 Allotment. • • • • • • • • • 0 • • • 0 16 Feeding. • • • 0 • • .. • 0 • • • 0 • 0 17 Grain Processing Methods • • • 0 • 0 • 17 Data Obtained. • 0 0 • • 0 • • • • • 0 17 Net Energy Determination 0 • .. • • D 0 19

Trial I • 0 • • • • • • • • • • • • • • • • 19 Allotment. 0 0 • 0 • 0 • 0 • 0 • • • 19 Feeding. 0 • 0 0 0 0 0 0 0 0 •I I ,) . 0 0 0 21 Processing 0 .. 0 0 0 • 0 0 • • • • 0 0 23 Data Obtained. • 0 0 •· • • • • • 0 0 0 24 Net Energy Determination 0 0 • • • 0 • 25

Trial II. 0 0 • 0 0 0 • • • • • 0 • • 0 • 0 27 Allo.tment. 0 0 • 0 0 0 0 0 • • • • • • 27 Feeding. • • • "· 0 0 0 0 0 0 • 0 • • • 29 Processing. 0 • 0 • • " 0 • • • • 0 • 31 Data Obtained. • 0 0 0 • • • • • 0 • • 32 Net Energy Determination • • 0 • • 0 • 32

Trial III 0 • 0 0 • 0 0 " • • 0 • • • • • • 35 Allotment. • • • • 0 • • 0 • • • • • 0 35 Feeding. • • • • 0 • • 0 0 • • • • • • 36 Processing • 0 • • • • 0 • • • • • • • 38 Data Obtained. 0 • • • • • 0 • • • 0 • 39 Net Energy Determination • 0 • 0 • • • 40

IV. RESULTS. 0 • • 0 • • • • 0 • • • • • • 0 • • 0 • 42

Trial I 0 • • • • • 0 0 • • • 0 • • • 0 • 0 42 Feedlot Performance. 0 0 0 • 0 • 0 • 0 42 Net Energy 0 0 • 0 0 0 0 0 • • • • • 42

v

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TABLE OF CONTENTS (Continued)

Chapter Page

Carcass Merit. • • • • 0 0 0 0 0 • • .. 44 Trial II. • 0 0 • • • • • 0 • .. 0 .. .. .. 0 • 44

Feedlot Performance, • 0 • 0 • • 0 • Q 44 Net Energy • • • • • • • • .. • 0 • • II 48 Carcass Merit. • • • .. Ill • 0 • 0 • " • 50

Trial Ill • 0 • • • • • • • • • • • • • • • 50 Feedlot Performance. • 0 0 • • 0 .. • • 50 Net Energy • .. • • • • • • • • • • • • 53 Carcass Merit. • 0 • 0 0 0 0 • " • • • 53

v." DISCUSSION • • • 0 0 0 • • • • 0 • • 0 .. • 0 • 0 56

VI. SUMMARY. • • • 0 0 0 a • • • • 0 0 0 • • 0 0 0 0 63

LITERATURE CITED,. 0 0 0 0 0 • 0 • 0 • 0 0 0 0 • • 0 • 0 66

vi

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LIST OF TABLES

Table Page

lo Milos Fine vs. Coarse Grinding. o •• • 0 • 0 3

11. Comparisons of Rolled Milo ••••••• o • o • 5

111. Digestibility of Dry Rolled vs. Finely Ground Mi lo. • • • • • • • o • • • • • • • • .. • o • 6

IV. Comparisons of Pelleted Grains •• o o o o o o o 7

Steam Flaked vs. Ground Grains. • 0 0 0 0 • " 0

9

VI. Steam Flaked vs. Dry Rolled Grains.. • • • • • • 11

VII. Effect of Flatness of Flake of Steam Processed Milo on Dry Matter Disappearance From Nylon Bags,%. • • • • • • • • • • • • • • • • • • 12

VIII. Comparison of High Moisture Grain Processing Methods • • • • • o • , • • • • • • • • • • • 13

IX. Trial la Experimental Design Showing Number of Animals per Treatment •••••• o • • • • • 20

Xo Trial Ia Ration Composition. " . . 0 0 0 0 0 0 21

XI. Trial Is Percent Proximate Analyses of Feeds on Dry Matter Basis ••••••••••• o • 22

XII. Trial ls Analyses of Variance. • • • • • • • • 24

XIII. Trial ls Particle Size and Density of Processed Milo ••••••••••• , • • • •

XIVo Trial Ila Experimental Design Showing Number

26

of Animals per Treatm.ento o •••••• o • o 28

xv. Trial Ile Ration Composition. 0 0 • • • 0 0 • 29

XVI. Trial Ils Percent Proximate Analyses of Feeds on Dry Matter Basis. • • • • • • • • • • • • 30

XVII. Trial lls Analyses of Variance. e O O O 0 0 0 33

vii

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Table

XVIII a

LIST OF TABLES (Continued)

Trial IIs Particle Size and Density of Processed Milo o o o o o o o o •

Page

• • e O O 34

XIXo Trial Illa Experimental Design Showing Number of Animals per Treatmento o o o o • o • • • • 35

xx .. Trial Ills Ration Composition •• o o o o • D O 36

XXI. Trial Illa Percent Proximate Analyses of Feeds

XXIlo

XXIII.

XXIVo

XXVc

XXVI.

XXVIIo

XXVIII o

XXIX.

xxx 0

XXXI.

XXXIIo

on Dry Matter Basis •••• o • o ~ • , o • • 37

Trial Ills Analyses of Variance, o & 0 0 0 0 I

Trial Illa Particle Size and Density of Processed Miloo o •• o •• o • o o o o 0 0 O

Trial la Feedlot Performance (189 Days). • 0 •

Trial Is Net Energy Values of Processed Milo •

Trial Is Carcass Merit • • 0 0 0 .. • 0 • • 0 0

Trial Ila Feedlot Performance. 0 0 0 0 • • • • Triai· I Is Net Energy Values of Processed Miloo

Trial Ila Carcass Merit. 0 0 0 0 0 0 • 0 0 • 0

Trial III a Feedlot Performance (112 Days). 0 0

Trial Illa Net Energy Values of Processed Milo,, 0 0 0 0 0 0 0 • 0 • 0 0 • • • • • • 0 0

Trial Illa Carcass Merit • • 0 0 II • • • • 0 0

viii

39

41

43

45

46

47

49

51

52

54

55

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CHAPTER I

INTRODUCTION

Sorghum grain is the primary source of cereal grain

for fattening cattle in the Southwesto With the continued

influx of large feedlots into this area 0 the importance of

sorghum grain will be even more pronouncedo

Currently considerable emphasis is being placed upon

grain processing to increase efficiency of feedlot produc=

tiono Chemical composition of sorghum grain shows that its

potential energy is equal to corn and superior to barleyo

However 9 because of the lower starch availability, the

energy potential of sorghum grain has not been reachedo

Many fattening rations contain as much as 85% sorghum

graini ~ny improvement in feeding value of the grain would

be of co.ns.iderable e,c,onomic .. importance to the cattle feeding

industryo

Previous research indicates that the feeding value of

sorghum grain can be improved by both high moisture har­

vesting and reconstitutingo The purpose of this study was

to evaluate several methods of high moisture processing of

sorghum grain for fattening cattle.

Processing methods were evaluated on the. basis of

feedlot performance 9 carcass merit and net energyo

1

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CHAPTER 11

REVIEW OF LITERATURE

Methods of Processing

It is generally agreed that some processing of grain

is necessary to improve mixing and feeding value over whole

grain. The grain coat of milo is extremely resistant to

digestion; it is .,absolutely necessary to rupture. the grain

before feeding 9 as cattle apparently chew milo very little

in the normal mastication process. This is in contrast to

corn which can be fed whole with a fair degree of success,

as considerable portions of the grain.are broken by chewing

prior to swallowing (Hale and Taylor, 1965).

Grain processing methods have been critically.reviewed

the past few years to determine which methods have the

greatest potentia:l,for improving rate of gain and feed

efficiency for finishing cattle. For 'this review, the

available research data comparing methods of processing

have been sununarized to allow a concise evaluation of the

different methodso The methods of processing to be consid­

ered in this review i.ncludea grinding, dry rolling, steam

rolling, pelleting, steam flaking and high moisture

proce~sing.

2

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Grinding

Coarse grinding has been commonly used as a control

method to which other processing methods are compared.

There is a very large variation in grain designated as

coarsely groqnd; size of hammer mill screens used to produce

coarsely ground grain has varied from 3/16 in. to l/2 ino

Grains designated as finely ground were produced by using

hammer mill screens that varied from 1/8 in. to 1/4 ino

A summary of 10 trials comparing finely ground milo to

coarsely ground milo is shown in Table 1. Daily gain was

not greatly affected by fineness of grindo Fine grinding

milo decreased feed intake 5%; therefore. efficiency of

gain was improved by 5%.

MlLOs

Processing Method Treatment Control Method Method

Fine grinding

Coarse grinding

TABLE l

FINE VS. COARSE GRINDING

Percent of Control Methoda No. D~ily Daily Feedllbo Trials Gain Feedb Gain

101 95 95

aTreatment method is expressed as% of the control method.

bFeed intake and feed efficiency data on 90% dry matter ba.siso

c(l) 0 (10) 9 (16) 9 (46) 0 (53), (54), (59) 0 (67), and (68). \• i1-1··. ;-·

3

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Totusek ~ al. (1964) reported a 5% improvement in

feed efficiency with no significant difference in rate of

gain when finely ground milo was compared to coarsely

ground miloo They concluded that since the finely ground

milo was consumed in smaller quantitiese the energy in the

finely ground milo was more efficiently utilized, and less

fe.ed was required to satisfy the daily, energy requirement

of the calves than was true of the coarsely ground milo.

Fine grinding exposes a tremendous surface.area of

the starch portion .of the grain to rumen microorganisms ..

and .d.igestion in the small intestine. However 0 fine.ly

ground.grains are dusty and blowing might be a problem in

some. areas.

Dzy Rolling

4

Results of thre'e trials (Table II) indicated no advan­

tage for finely roiled milo over coarsely rolled milo. The

grain particles resulting from rolling may be multi-fractured c

-and therefore suscep~ible to the entry of enzyme-containing

fluid for digestion (Totusek 9 1968). · If the same amount of

surface area (due to'multi-fracturirig) is available. in dry

rolled grains as in finely ground grain 9 this.would explain

the similarity in feeding value of the two processing

metho.ds. Data summarized from five-trials indicated.an.ly

a slight advantage in efficiency for fine grinding. over dry

rolling3. however, rate of gain was reduced 2% by fine

grinding.

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TABLE 11

COMPARISONS OF ROLLED MILO

Percent of Control Methoda

5

' Processing Method Treatment Control

· Method Method No. Daily Daily Feedllb. Trials Gain Feedb Gainb ·

Fine Coarse , rolling rolling 96 97 100

Steam Dry rolling rolling 98 100 102

Steam , rolling Grinding 98 99 101

,Ory Fine rolling grinding 98 97 99

aTreatment method is expressed as% of the control method. bFeed intake and feed efficiency data on 90% dry matter basis.

c(l2) and (13). ' d(6), (61), (64), and (65),

' e(53), (54), and (56). , ,f(l), (16), (26), (46), and (59).

Mehen ~ .!!.!.• (1966) reported no significant differences

in digestion of the important fractions of dry rolled or

finely ground milo as seen in Table Ill.

Conventional steam rolling involves subjecting the

grain to steam for about 3 to 5 minutes prior to rolling •

. Temp.eratures 1 of approximately 180° F. are obtained in such

a'.process (Hale and Taylor, 1965). ,A summary of .four trials

(Table 11) indicated that conventional steam rolling is

Page 15: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

6

actually inferior to dry rolling in that rate o.f gain and

feed efficiency were 2% lower for steam rolled milo. Con-

ventional steam rolled milo was of slightly lower value than

ground milo due to lower efficiency of utilization and

reduced rate of gain.

TABLE III

DIGESTIBILITY OF DRY ROLLED VS. FINELY GROUND MILOa

Item

Dry matter Protein True protein Ether extract Crude fiber Nitrogen free extract Total digestible nutrients Gross energy

aMehen ~ sl• (1966).

'\\ •.. ---i-,

Pelleting

Dry Finely Rolled Ground

% % 68.8 70.7 58.7 59.4 80.6 81.4 75.6 72.1 43.3 31.6 76.3 78.5 69.3 70.3 67.3 68.8

- Pelleting a fatt·ening type milo ration will strikingly

improve feed efficiency as indicated by a summary of 12

trials in Table IV. Totusek (1968) reported that pelleting

finely groundp coarsely ground and steam rolled milo

improved total feed efficiency 5. 1%, 9. 2%, and 6 .• 8%,

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7

respectively, over the unpelleted milo when rations con-

tained 50% milo and 32% roughage. Rate of gain was not

affected, but dressing percentage was lowered 1% and carcass

grade was decreased slightly by pelleting. Similar results

were observed by Perry~!.!• (1961) when a pelleted fatten­

ing type corn ration that contained 20% hay was compared to

the same ration containing dry rolled corn. Feed efficiency

.was increased .. 6% by pelleting; however. carcass grade and

dressing percentage were lowered by pelleting.

TABLE IV

COMPARISONS OF PELLETED GRAINS

Processing Method Percent of Control Methoda Treatment Control No. Daily Dai lb Feedzlbo

Grain Method Method Trials Gain Feed Gain .

Milo Pelleting Grinding Sc 104 94 91

Dry 4d Milo Pelleting rolling 105 92 93

Corn Pelleting Grinding 3e 105 94 89

Dry 2f Corn Pelleting rolling 101 87 90

a.Treatment method is expressed as% of the control method. bFeed intake and feed efficiency data on 90% dry matter basis.

c(36) 9 (53), (54), and (56). d (52) o (55) 0 (57) 0 and {58).

e(24), (30), and {69)o f(24) and (51).

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8

Pelleting of corn has been more advantageous than

pelleting of milo. A summary of five trials comparing

pelleted corn to both ground and dry rolled corn (Table IV)

indicates a 10-11% improvement in feed efficiency and also L, an improvement in rate of gain due to pelleting.

Steam Flaking

The feed.ing value of grains definitely can be improved

by flaking. Steam flaking is more than just a method of

steam rolling; the idea of just applying steam and running

the grain through a roller mill apparently will not produce

a product that will bring about the advantages of flaking

over other methods of gra.in processing. Data in the past

indicated varied responses in feedlot gains and feed utili­

zation due to steam processing grainso Hubbard (1967)

contributed these variable responses to different conditions

involved in the grain processing. Hale and Taylor (1966)

outlined the key points to the steam processing method of

flaking milo as followss

lo Moisture of the grain raised to approximately

20 percent.

2o Grain enters rollers at 212° to 216° F.

3. Approximately .20 lb. pressure in the steam chamber.

4. Cold roller spacing of 0.003 inch.

5. Flake should we_igh 25 lb. per bu. (air-dry weight

basis).

6. Gelatinization of 30 .to 40 percent.

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9

A summary of 17 trials comparing steam flaked to ground

grains is shown in Table V. All grains show a consistent

improvement in feed efficiency, but a varied response is

indicated for daily gain. Milo showed the greatest overall

response to steam flaking in that rate of gain was increased

'10% and feed efficiency 8% compared to dry coarse grinding.

TABLE V

STEAM FLAKED VS. GROUND GRAINS

Processing Method Treatment Contr.ol

Percent of Control Methoda No. Daily Daily Feed/lb.

Grain Method Method Trials Gain Feedb Gainb

Steam Coarse 7C .Milo flaking grinding 110 101 92

Steam 5d Corn flaking Grinding 98 92 91

Steam Coarse 5e Barley flaking grinding 100 95 95

aTreatment method is expressed as % of the control method. bFeed intake and feed efficiency data on 90% dry matter

ba:siso c

' (20)9 (46)9 and (67). d(24), (30), (34)P and (44). ·e(20).

Steam flaking of corn increased feed efficiency 9%,

· but reduced rate of gain and feed consumption 2% and 8%,

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10

respectively, as compared to coarse grinding of dry shelled

corn.

Barley has shown less response to steam flaking than

either corn or milo. Steam flaking of barley improved feed

efficiency·5%, reduced. feed consumption 5%,.and did not

affect rate of gain compared .. to dry grinding q

In comparing._ steam flaked grains to dry rolled grains

(Table VI), milo and_corn were markedly impr.o.ved for the

ec.onomically important traits (i.e. 9 rate of gain and feed

~fficiency) by steam flaking. Steam flaking of milo

increased rate of ga.in 8%, feed consumption 3%, and feed

efficiency 4% over dry rolling of milo as indicated by a

summary of six trials.

A summary of eight trials comparing steam flaked corn

to dry rolled corn shows an. advantage in rate of gain (5%)

and feed efficiency (8%) for steam flaked corn. The daily

consumption of flaked corn was reduced 3%.

As previously shown in Table V 9 the feeding value of

barley has not been improved to the.extent of milo and corn

by steam flaking.· A summary of five tria1s- comparing

steam flaked and dry rolled barley indicated no. advantage

in feed efficiency for steam flaked .barley. Rate of gain

was improved.4% and feed consumption was .increased 3% by

steam flaking barley.

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11

TABLE VI

STEAM FLAKED VS. DRY ROLLED GRAINS

Method Percent of Control Method a Process in~ Treatment Control No. Daily Dai lb Feedllbo

Grain Method Method Trials Gain Feed Gain

Steam Dry ')

Milo flaking rolling 6c 108 103 96

Steam . Dry gd Corn flaking rolling 105 97 92

Steam Dry 5e Barley flaking rolling 104 103 100

aTreatment method is expressed as% of the control method. QFeed intake and feed efficiency data~on 90% dry matter basis.

c(l9) 9 (2l)o (22)P and (46). 4(19)9 (24) 9 (32)o (33), and (35). e . . ( 19) 9 (21), and (22) •

Hale~~· (1965) reported the flatness of flake was

very important to the utilization of steam flaked milo. The

results of nylon. bag studies to determine the disappearance

of dry matter from dry rolled and steam flaked milo samples

are shown in Table VII. Matsushima and Montgomery (1967)

reported heifers fed "thinu .(1/32 in.) corn flakes gained

4.6% faster and 7.8% more efficiently than those fed "thick"

(l/12 in.) corn flakes. The two types of flakes were pro-

duced by varying the roller spacing.

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TABLE VII

EFFECT OF FLATNESS OF FLAKE OF STEAM PROCESSED MILO ON DRY MATTER DISAPPEARANCE FROM NYLON BAGS, %a

12

.-,-· ----------------------------Hours in

Rumen

2

4

6

8

24

aHale il llo

Dry Rolled

2lo4

23o2

27.0

31.9

54.5

(1965)0

Poorly Rolled

23o9

20o9

23.6

29o9

55o0

Regular Rolled

44o7

37.8

44o4

4lo4

6609

Flat Rolled

49al

4408

47o4

49.4

7106

Parrott il .!lo (1967) reported that steam flaking of

barley does not improve the digestibility of the various

proximate fractions on the available TDN. Also, digest-

ibility of barley was not influenced by the degree of flak-

ingo

High Moisture Processing

High moisture processing of grain includes high mois-

ture harvesting and reconstituting of dry graino The

moisture level is normally in a range of 25-35%, High

moisture grain must be stored under anaerobic conditions

to prevent spoilage. Also, for maximum utilization, the

grain must be processed before or after storage.

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High moisture processed grains have markedly improved

feeding value over dry processed grains as indicated by a

summary of 36 feeding trials shown in Table VIII. The

improvement in feeding value is consistent for both high

moisture harvested grains and reconstituted grains.

TABLE VIII

COMPARISON OF HIGH MOISTURE GRAIN PROCESSING METHODS

Percent of Control Methoda

13

Grain

Processing Method Treatment Control Method Method

No. Daily Daily Feedllb. Trials Gain Feedb Gain

Miloc Recon.

Cornd Recon.

Milo~ HMH

She 1 I,ed .HMH corn

Grd. ear corng · · HMH

Dry process

Dry process

Dry process

Dry process

Dry process

101

104

100

100

103

93 92

104 95

90 90

93 95

94 92

aTreatment method is expressed as % of the control method .• bFeed intake and feed efficiency data on 90% dry matter basis.

c(37) 0 (46) 0 (47), and (67). d(2), (28), and (29). e(8) 0 (9) 9 (10), (11), {38), and (47). f .

(2) 0 (7) 0 (35) 9 and (50). g{3) 11 (4), (5) 9 (7) 9 (15), (17), and (18).

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14

Reconstituting of corn improved feed efficiency 5% and

rate of gain 4% compared to dry processed corn in a summary

of seven trials.

The efficiency of gain was improved 8% and rate of gain

1% by reconstituting milo, compared to dry processed milo.

A summary of three trials with reconstituted sorghum grain

by McGinty ~ s!.l• (1968) indicates a

lo Time of grinding reconstituted sorghum grain is

important if maximum benefits of the process are

to be obtained. Grinding the dry grain before

addition of water gave no improvement in feed

efficiency over dry ground grain.

2. !n ~ and .i!l vitro results indicated no benefit

for sorghum grain reconstituted near freezing

temperatures.

3o There was no difference in feed efficiency between

reconstituted grain stored 10 or 20 days.

4o Differences in initial water temperatures had no

significant influence on efficiency of feed con­

version.

5. Cattle fed sorghum grain reconstituted in whole

form at warm temperatures required 10-20% less dry

matter per pound of gain than did cattle fed dry

ground grain.

High moisture harvested milo was utilized 10% more

efficiently than dry milo in a summary of 10 trials. The

improved efficiency was apparently the result of increased

Page 24: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

digestibility of the milo as there was no difference in

rate of gaina The digestibility of dry matter, organic

matter, nonprotein organic matter and energy was signifi­

cantly higher for reconstituted milo than for finely or

coarsely ground milo (Buchanan-Smith~!.!,., 1968).

High moisture harvested shelled corn was utilized 5%

more ,efficiently, with no advantage in rate of gain, as

compared to dry shelled corn. Feed consumption was 7%

lower for the high moisture harvested shelled corn.

15

High moisture harvesting has shown more promise for

increasing rate of gain and feed efficiency for ear corn

than for shelled corn~ A summary of seven trials comparing

high moisture harvested ground ear corn to dry ear corn

indicated an advantage of 3% in rate of gain and 8% in

feed efficiency for high moisture harvested ground ear corn.

Page 25: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

CHAPTER tII

MATERIALS AND METHODS

General

Three trials were conducted to determine the effect of

grain processing method on the feeding value of milo for

fattening beef cattle. Evaluation of the processing method

was by feedlot performance, carcass merit and net energyo

The three trials will be identified as followss Trial I -­

Stillwater0 1967-68; Trial 11 -- Fort Reno, 1967-683

Trial Ill -=-Stillwater 0 1968. Experimental procedures

comm.on to all three trials will be discussed under the head­

ings of allotment 0 feeding 9 grain processing methods, data

obtained 0 and net energy determination 0 followed by a dis­

cussion of procedures specific for each trial under the

same headings.

Allotment

Anguso Hereford, crossbred (Angus X Hereford) steers,

and Angus and Hereford heifers were used in Trial lo Cross­

bred (Angus X Hereford) and Hereford steers were used in

Trial II. The calves in Trials I and II were from the

University experimental herds located at the Fort Reno

Station and the Lake Carl Blackwell Range. Hereford, Angus,

and crossbred (Angus X Hereford) heifers were used in

16

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17

Trial lllo These heifers were purchased at the Oklahoma City

Stockyardso A randomized complete block design was used in

all trialso The calves were blocked on the basis of weight 0

sex and condition score and randomly assigned to treatment

within each block.

Feeding

In all three trials, a high concentrate ration of 90%

concentrate and 10% roughage was fed ~q libitumo

All animals had access to an open=sided shed, outside

lot and automatic waterers with thermostatically controlled

heating during the wintero

Grain Processing Methods

Finely and extra finely ground milo were produced with

a hammer mill, using 3ol8 and 1.59 mm. screens, respectively.

Dry rolled milo was produced by rolling air-dry whole milo.

with a roller tolerance in excess of 0.076 mm.

Reconstituted milo was obtained by adding water to the

air-dry grain to raise the moisture to the appropriate level

and then stored in oxygen-free conditions for at least 21

days. Prior to feeding 0 the high moisture harvestedtand

reconstituted milo were either ground througl) a 3o 18 mm.

screen or ,rolled with approximately 0.076 mm. tolerance

between the rollers.

Data Obtained

Performance data obtained were average daily gain,

average daily feed lntakep and feed per kg. of gain calcu­

lated both on a live shrunk weight basis and on an empty

Page 27: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

18

body weight basis~ Empty body weight gain per kg, of feed

and energy gained per kgo of feed were calculated so that a

comparison of weight gain and energy gain pould be made.

Daily feed consumption records were kept. Initial and final

weights were taken after a 16 ... hour shrink off feed and water.

Intermediate weights were taken at 28-day intervals with'

water removed 16 hours prior to weighing.

All animals were slaughtered at the end of the feeding

trials. Rumen weights, both intact and empty 0 were taken

to allow calculation of rumen content. Following a 24-hour

chill, carcass data obtained included carcass grade 0

marblingo ribeye area, fat thickness over the ribeye,

chilled carcass weight and percent kid:p.ey fat. From this

data, dressing percentage and cutability were calculated, 1

The right side of the carcass was then quartered, weighed

first in airo and then in water to allow calculation of

carcass specific gravity.

Dry matter of feeds was determined several times during

each 28-day period o These determinations wer.e averaged and

used to adjust ration treatments to an equal dry matter

1cutability 9 or percent boneless retail cut yield, was estimated by the equation of Murphey et al. (1960) 9 which iss <' .,·. - -

Y=5lo34~(5,78 x A)~(~.462 x B)+(0.740 x C)~(0,0093 x D) where.s

Y=boneless retail cuts, as% of carcass A=avera~e fat thickness over rib~ye (ino) :S=%_ kiqney · fat · C=ribeye area {sqo in.) D=chilled carcass weight (lbo).

Page 28: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

content. Grains were sieved and weights per bushel were

taken to characterize the processed grains as to particle

size and density, respectively.

19

Duncan°s New Multiple Range Test (Steel and Tor.rie,

1960) was used to compare treatment means whenever a signif­

icant F value was obtained.

Net Energy Determination

A representative slaughter sample was used to estimate

the initial composition of the experimental animals.

The weight of the rumen contents was subtracted from

live shrunk weight to obtain empty body weight. Carcass

specific gravities were calculated by dividing carcass

weight in air by carcass weight in air minus carcass weight

in water.

After completing the feeding trial, all animals were

slaughtered and subjected to essentially the same procedure

as described for the slaughter group.

All net energy calculations and equations used for body

composition are the same as those used by Newsom (1968).

The net energy of each type of processed milo was

calculated by using the mean values for each animal within

each treatment. A computer program was used to make all

net energy calculations,

Trial I

Allotment

Fifteen Angus steers, six Hereford steers, three

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20

crossbred (Angus X Hereford) steers, six Angus heifers and

six Hereford heifers, averaging 187.7 kg., were started on

trial November 16, 1967 to compare three types of processed

milo fed in a high concentrate ration. The experimental

design is shown in Table IX.

TABLE IX

TRIAL 18 EXPERIMENTAL DESIGN SHOWING NUMBER OF ANIMALS PER TREATMENT

Processed Milo Finely Recon.- Ground- Total

Blocks Ground Ground Recon. Number

l 4 4 4 12

2 , .•.

4 4 3a 11 ,,,.

3 4 ...!t ..1.a .ll -12 12 10 34

aOne steer died of bloat, '

The calves were first separated into groups of three

according to sex, breed 9 weight and age of dam and sire.

The treatments were then randomly assigned within these

groups of threeo The 12 heaviest Angus steers, the 12

heifers and the 12 lightest steers were placed in blocks

one, two and three, respectively.

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Feeding

The three types of processed milo -- finely ground,

reconstituted-ground and ground-reconstituted - were fed

in a 90% concentrate mixture. The non-milo ingredients in

21

the ration were combined into a premix. The composition of

the premix and the complete ration is shown in Table x. The

proxima.te analyses of the premix and the processed milo are

shown in Table XI.

TABLE X

TRIAL Is RATION COMPOSITION

Ingredient Percent

Milo

Dehydrated alfalfa pellets (17% C.P.)

Cottonseed hulls

Cottonseed meal (41% C.P.)

Urea (45% Nitrogen)

Salt

Steamed bonemeal

Added per lbo of ration Vitamin A Aureomycin

2040 5

83.0

6.4

4,2

4.2

1.0

0.6

0.6 100.0

1.u. mg •.

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TABLE XI

TRIAL Ia PERCENT PROXIMATE ANALYSES OF FEEDS ON DRY MATTER BASIS

Feedstuff Dry a Ashb Crude b Ether b Crudeb NoF,E .. Matter Protein Extract Fiber.

Milo Finely ground 87o4 lo43 9o92 3o55 2o80 82030

Recon.-ground 7108 0.89 8053 2.70 1.85 86.03

Ground=recon. 6706 0.95 8068 3.25 2.00 85.12

Premix 90o5 11055 29073 6035 22.80 29.57

aAverage of 24 determinations.

bAverage of two determinationso

clOO - (sum of values reported for ash 9 crude protein 9 ether extract and crude fiber).

c

N N

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23

The calves were started on feed eight days before the

trial begano For the first five days of the pretrial feed~

ing period 0 the calves were on a starter ration consisting

of 5000% finely ground milo, 2000% dehydrated alfalfa pel­

lets, 5.0% cottonseed meal 0 2400% cottonseed hulls, Oo5%

salt and Oo5% bonemealo The last three days of the pretrial

period 0 the calves were gradually changed over to the test

rations.

The three rations were fed daily in quantities to

assure availability of feed until the next feeding., Uncon­

sumed feed was weighed back frequently to assure that fresh

feed was available at all times. The reconstituted-ground

milo was ground daily except that enough was processed on

Friday to supply the amount needed over the weekend.

Processing

The ground-reconstituted milo was produced by adding

water to the air=dry 11 finely ground milo to raise the mois­

ture level to 30%0 The ground-reconstituted milo was then

stored in oxygen=free conditions for at least 21 days prior

to feeding.

The reconstituted-ground milo was produced by adding

water to the air-dry whole milo to raise the moisture level

to 30%. The reconstituted whole milo was then stored in

oxygen-free conditions 21 days and then ground just prior

to feeding.

All of the reconstituted grain was stored in airtight

plastic bags with 4008 kg. per bago

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24

All milo used in this study was obtained from the

Stillwater Milling Company in one or two ton quantities as

neededo

Data .. Obtained

The experimental animals were slaughtered on May 22,

1968 after 189 days on feed. All variables 0 including per-

formance .data, carcass data and net energy values, were

subjected to a hierarchical analysis of variance and an abbre ...

viated Doolittle as described by Newsom (1968)0 Analyses

of variance components are shown in Table Xllo

TABLE XII

TRIAL ls ANALYSES OF VARIANCE

Source df

For Feed lntake 9 Feed/Kg. Gain and Net Energy Valuess Total 8 Blocks 2 Treatment 2 Blo.ck X Treatmenta 4

For Average Daily Gain and Carcass Dataa Total Blocks Treatment Block X Treatmenta. Within pen

aError term used to test treatments.

33

2

2

4

25

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25

Two c.alves on the ground-reconstituted milo died during

the experiment due to bloat. The feed records were adjusted

by subtracting the estima1:;.~d intake of the dead calves, which

was theaverage intake of the four calves in the pen, from

the total pen intake.

After the trial was st~rted, one steer on the finely

ground milo was found to have one testicle. His average

daily gain and feed required per kgo of gain were adjusted

to a steer equivalento 2

Table XIII illustrates the relative density and particU3

size of the processed milo fed in Trial I.

Ne.t Energy Determination

The slaughter group used for estimating initial compo­

sition was the same as for Trials III and IV (Newsom, 1968).

For the 34 experimental animals which completed the

test in Trial I, rumen weights were taken to the nearest

one-fourth lb o. The carcass quarters were weighed in air to

the nearest one-fourth lb. and the quarters of the right

side were weighed in water to the nearest five gm.

Th~ NEm+p and NEm values of the premix were estimated

2using data taken from a trial at the Fort Reno station coi;npar.ing steers O bulls and heifers (Tanner §!.:!:. li•, 196 7), a correction factor (C.F.) was obtained.

~g ~~~~~s i:Si ~:,: = 0.854 (C.F.)

The actual average daily gain of the animal in question was multiplied by this C.F. to obtain his adjusted ADG. His intake was divided by the adjusted gain to obtain adjusted feed per kg. of gain.

Page 35: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

Process

Finely _ground

Recon. -ground

Ground-recon.

TABLE XIII

TRIAL Ia PARTICLE SIZEa AND DENSITY'b OF PROCESSED MILO

3.18

0

7~1

1.5

Screen Size (nun.) 2.12 1.41· lo02 0;35·-

Percent Retained on Screen

Oo2

27.l

2.2

8.4

28.3

17.8

37.2

14.6

37.9

32.4

17.6

34.4

lb. thru per

..... 0 • .3.6 ..... ·----·" ....... bu ......... .

21.8

5.3

6.2

42~7

26.4

34.9

aParticle sizes Four 100 gm. samples of each grain were sieved.

bTest weights.reported are the average of six determinations and are on a 90% dry matter basis •.

N 0\

Page 36: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

to be 918.9 (Morrison, 1959) and 1069.6 (Lofgreen and

Garrett, 1967) kcal. per kg., respectively.

27

Since feed intake was on a pen basis, net energy values

are valid only for a pen of animals. The computer program

was.designed to use the mean intake of a pen of animals to

compare with the caloric gain and maintenance requirement of

each animal. The net energy values were then averaged for

each treatment.

Trial ll

Allotment

Seventy-four Hereford steers and eight crossbred

(Angus X Hereford) steers, averaging 230.9 kg,, were started

on trial December 12, 1967 to compare seven types of proc­

essed.milo fed in a high concentrate ration. Twelve head

were on each treatment, in four pens of three head each,

~rranged in a randomized block design as shown in Table XIV.

The 84 calves were.selected from a total of 90 head,

The 90 head were plotted on graph paper with shrunk weight

. and condition. score as the X and Y axes, respectively, and

then divided by diagonal lines into two blocks, with the

heaviest steers and the hi'gttest; condition score making up

one block and the lightest steers with the lowest condition

score making up the second block. The six calves with the

lowest condition score and lightest in weight were not put

on trial.

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TABLE XIV

TRIAL Ils EXPERIMENTAL DESIGN SHOWING NUMBER OF ANIMALS PER TREATMENT

Processed Milo Coarsely Finely Extra HMH- HMH- Recon.- Recono-

Blocks Rolled Ground Finely Ground Rolled Ground Rolled .. Ground

1 6 6 6 6 6 6 5a

2 6 6 5a 5a 6 6 6

aOne steer died.

Total Number

41

40 -81

N 00

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29

Feeding

The seven types of processed milo were fed in a 90%

concentrate mixture. The non-milo ingredients in the ration

were combin.ed into a premix. The composition of the premix

and the complete ration is shown in Table XV. The proximate

analyses of the premix and the processed milo are shown in

Table XVI.

TABLE XV

TRIAL II I RATION COMPOSITION

Ingredient Percent

Milo

Alfalfa hay, chopped

Cottonseed hulls

Cottonseed meal

Ur.ea (42% Nitrogen)

Salt

Bonemeal

Added per lb. of ration Vitamin A Aureomycin

1500 5

83.4

6.0

4,0

4.0

1.0

1.0

o.6 100.0.

I.u. mg.

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TABLE XVI

TRIAL lis PERCENT PROXIMATE ANALYSES OF FEEDS ON DRY MATTER BASIS

Feed stuff Dry .. a b

Crude b Ether b c Ash Crudeb N.F.E. .... Matter Protein Extract Fiber

Milo Coarsely rolled 85.3 1.37 8,86 3.85 1.95 83.97

Finely ground 85.5 1.16 9.35 3.00 2.10 84.39 '

Extra finely ground 85.5 1.31 10.28 3,65 1.95 ,?2.81

HMH-ground 70.1 0.94 8.36 2.30 1.10 87.30

HMH-r9lled 68.9 0.91 8.06 1.80 0,85 88.38 ..

Recon,-ground 74,3 1.27 8.77 3.40 1.45 85.11

Recon. -rolled 73.4 1.03 9,05 2.90 1.40 85.62

Premix 90.9 10.82 36070 6.05 25.29 21.14

-aAverage of 24 determination!3.

bAverage of two determinations.

clOO - (sum of values reported for ash, crude protein, ether extract and crude fiper) .. w 0

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31

The steers were started on feed six days before the

trial began. The initial ration consisted of eight lbo of

test ration and four lb. of cottonseed hulls per head per

day. The test ration was increased gradually until the

steers.were receiving 13 lb. of test ration on the sixth

day following initial feeding.

The four "wet" grains, high moisture harvested-ground,

high moisture harvested-rolled, reconstituted-ground and

reconstituted-rolled, were processed daily, with the excep­

tion that enough was processed on Friday to feed over the

weekend.

The coarsely rolled, finely ground and extra finely

ground grains were processed, combined with premix, and

stored in one ton quantities.

The steers were fed once daily in sufficient quantities

to assure availability of feed until the next feeding. · Feed

was weighed to the nearest pound. Unconsumed feed was

we.ighed back and removed as necessary to assure fresh feed.

Dry matterdeterminations·were taken every 28 days and used

to adjust all rations to an equivalent dry matter content.

Processing .

The extra finely ground milo used in this trial was

produced by grinding dry mi lo through a hammer mill with a

1.59 mm. screen. The coarsely rolled milo was produced by

rolling .dry.milo through a roller mill to allow approximate­

ly 25% of the grain to fall through unbroken.

The reconstituted milo was produced by adding water to

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32

air-dry milo as it was augered into a 4.3 X 8,2 m.Harvestore

glass-lined .. , airtight silo, .. The moisture content was raised

from 14 to 28%,

The high. mo.is.ture harvested milo was combined at

appr.oximat.ely .3.2% moisture, After harvesting, it was stored

in a 4,3 X 8.2 m. Harvestore glass-lined, airtight silo,

All milo used in this study was of the variety Northrup

King 222 and was grown on the Fort Reno station.

Data Obtained

The steers were slaughtered on two different days after

an av.erage. of 174 days on feed,

Thr.ee .steers died due to bloat during the feeding

trial11 one.each.on reconstituted-rolled, high moisture

harvested~ground .. and extra finely ground milo. The feed

reco.rds .were ..... adjusted by subtracting the estimated intake

of the deceas.ed steer, which was the average intake of the

three .. calves .in the pen, .from the total pen intake,

Analysis of variance procedures were the same as those

for Trial I (page 24). Variance components are shown in

Table XVII.

The relative density and particle size of the proc-

essed milo are shown in Table XVIII.

Net Energy Determination

The a.laughter group and the procedures for net energy

determina.tion were the same as those used for Trial 1

(page .25),

The NEm+p and NEm values of the premix were est~~ated

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33

to be 839.3 (Morrison, 1959) and 970.4 (Lofgreen and Garrett,

1967) kcal. per kg., respectively.

TABLE XVII

TRIAL Ila ANALYSES OF VARIANCE

Source df

For Feed lntake 0 Feed/Kg. Gain and Net Energy Valuess Total 13 Blocks Treatments Block X Treatmenta

For Average Daily Gain and Carcass Datas Total Blocks Treatment Block X Treatmenta Within pen

aError term used to test treatments.

1

6

6

81 1

6

6

68

Page 43: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

,f·f:,,

TABLE XVIII

TRIAL Ilg PARTICLE SIZEa AND DENSITYb OF PROCESSED MILO

~., ,. •"··~·----.

Process Screen Size {rmno} lb. 4.76 3ol8 2ol2 le41 lo02 0.36 thru per

• ••• ,c< .o.c36. ·•·· • cbu •...

Percent Retained on Screen

Coarsely rolled 0 33.40 59.80 5.80 0.62 0.17 0.18 53.3

Finely ground 0 0.14 0.90 9.64 18.10 32.60 38.60 44.7

Extra finely ground 0 Oo12 0.12 0.39 4.17 28.60 66.60 40.8

HMH-ground 0 0.56 1.90 5.90 9.00 18.30 64.30 31.3

HMH-rolled 6.20 26~60 19.80 7.80 3.30 9o00 27.20 27.8

Recon.-ground 0 0,19 1.00 11.60 14.70 22.80 49.70 37.6

Recon.-rolled 4.60 27020 24.30 10090 3.80 7.20 22.00 30.1

<:lParticle .sizeg Four 100 gm. samples of each grain were sieved.

bTest weights reported are the average of four determinations and are on 90% dry matter basis.

w +:"'

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35

Trial Ill

Allotment

Twenty-two Angus 0 twenty Hereford and eight crossbred

{Angus X Hereford) heifers were started on trial July 2 9

1968 to compare five types of processed milo in a high con-

centrate ration. The initial weight of the heifers was

170.,9 kg., The experimental design is shown in Table XIX.

Blocks

1

2

TABLE XIX

TRIAL Illa EXPERIMENTAL DESIGN SHOWING NUMBER OF ANIMALS PER TREATMENT

Processed Milo Dry Recon.- Recon.- Recon ... Recon.-Rolled 22% 30% 38% 38%-1-day

5 5 5 5 5

5 5 4a 5 5

Total Number

25

24

49

aOne heifer died after completion of performance data; pen average used in calculating net energy values and carcass data a

The three groups (Angus, Hereford and crossbred

heifers} were blocked independently into two blocks based

on weight and condition, using the same method that was

used in Trial 11 {page 27). Two Angus heifers were

Page 45: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

selected at random to put in the crossbred group.

Feeding

The five types of processed milo were fed in a 90%

36

concentrate mixture. The non-milo ingredients in the ration

were combined into .a premix. The composition of the premix

and the complete ration is shown in Table xx. The proximate

analyses of the premix and the processed milo are shown in

Table XXI.

TABLE XX

TRIAL Ills RATION COMPOSITION

Ingredient Percent

Milo

Dehydrated alfalfa pellet crumbles (17% C.P.)

Cottonseed hulls .

Soybean meal crumbles (44% C~P.)

Urea (45% Nitrogen)

Salt

Bonemeal

Added per lb. of ration Vitamin A Aureomycin

1600 5

84.00

4.93

4.93

4.30

0.64

0.60

0.60

100.0

1.u. mg.

Page 46: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

TABLE XXI

TRIAL Ills PERCENT PROXIMATE ANALYSES OF FEEDS ON DRY MATTER BASIS , .. ,_, .................. ~ .. -.• •-• ....... _ _, ,-. _,. """ ' • -·- ~-. "•'• •-Tu• j,,, ·~'- "-' ,a '"

Feedstuff

Milo Dry rolled

Recon.-22% --

Recon,-30~ ·-- ("•i

Recon.-38%

Recon. -.38%-l"!".day

Premix

Dry b - Matter.~ Ash . , . ., .. '"- ., ~

86!9 1.34

77,3 1.08

68.6 0.77

62.0 0.61 ..

64.7 0.64

89.9 7.53

Crude b . Protein

10.84

10.51

7.74

8.45

7.75

37,46 ·,~__,,._,_. '0'' w• ,,. •• ""'""-S'°'n••·~·-·•- --· "••"-~'""•'"" ... ';,••"" .. · • ..., •• ,., •. ,.,,,,,,,, ''°'• ••••·,,, ••••_., ... • ''T' .-·,..-~ .. , ..... ~~- ••~•'• •••'•'•'."'"'""' OM" ••o

a Aver.age of. 16. deter.minations.

b . Aver.age of two deter.minat:ions.

Ether b Extract ..

3.11

3.08

2.79

2.41

2.41

2.31

. c N.F.E. C7'1deb

.. Fiber . _. .. _ .. ··-- ....

1.52 83.19

1.50 83.83

1.11 87~59

1.02 87.51

0.90 88.30

18.48 34.22 "'"'""" ,,-,-... ~-.· • W> .... ,,• '•"•'"••W•''O, __ ,,...,., •• , -••,•·•-•• --·-- 0 "•'•'" ~-- • •H •'S ,,,.•,• ,.,,.__ •

clO() .... (stlll\ o-f valti~s reported for ash, crude protein, E!ther [email protected] and crude fiber).

l..,J ~

Page 47: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

38

The calves were put on a starter ration consisting of

40% dehydrated alfalfa pellets, 10% cottonseed meal, 48%

cottonseed hulls, 1% salt and 1% bonemeal for 20 days prior

to the start of.the trial, At this point, the processed

milo was introduc.ed into the ration at the rate of 10%11

The milo was increased 5% per day until the calves were on

full feed.

The five rations were processed and fed daily in quan­

tities to assure availability of feed until the next feeding,

Unconsumed feed was weighed back daily and removed to assure

fresh feed was available at all times.

Processing

The. 22 and 30% reconstituted milo was produced by

adding the necessary amount of water to air-dry milo and

mixing in a cement mixer. The 38% reconstituted milo was

produced by soaking the air-dry whole milo approximately

10 hourso The 22, 30 and 38% reconstituted whole milo was

then stored under oxygen-free conditions in airtight

plastic bags.for 21 days or moreo

The reconstituted-1-day milo was produced by soaking

whole air-dry milo 12 hours and then letting it set in a

0.91 m. X 0,60 m. X 0.30 m, open container for 24 hours

prior to rolling and feeding.

All milo. used in this study was obtained from the

Stillwater Milling Company in one or two top quantities

as neededo

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39

Data Obtained

Performance data was sununarized after an average of 112

days on feed. The heifers were then subjected to measure­

ment by the K40 counter and ultrasonic equipment prior to

slaughter. The cattle were removed in two different groups,

with the heavy block being slaughtered first.

One heifer died of .heat stress following the K40 and

ultrasonic measurement. Her data is included in feedlot

perf ormanc.e O but a pen average was used in net energy and

carcass data. All variables were subjected to a factorial

analysis of variance. The analyses of variance components

are shown in Table XXII.

TABLE XXII

TRIAL Ills .. ANALYSES OF VARIANCE

Source df

For Feed· in.take, Feed/Kgo Gain and Net Energy Values, Total 9 Blocks Treatment Block X Treatmenta

For Average Daily Gain and Carcass Dc1;1.taa Total· Blocks rreatment Block X Treatmenta Within pen

aE~ror term used to test treatments.

l

4

4

48

l

4

4 39

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40

The relative density and particle size of the processed

milo are shown in Table XXIII.

Net Energy Determination

Six calves_were slaughtered to estimate the initial

caloric content .. of the experimental heifers in Trial lllo

The slaughter group was selected at random from the 56 head ..

The procedures used .for net energy calculations and

body composition were the .. same. as used by Newsom (1968) ..

The-NEm+p and NEm values of the premix were estimated

to be 97809 (Morrison, 1959) and 1108.9 (Lofgreen and

Garrett, 1967) kcalo per kg., respectivelyo

Page 50: FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT …

TABLE XXIII

TRIAL 111 s PARTICLE SIZEa AND DENSITYb OF PROCESSED MILO

·~, .. .,-,,~..-.. ~- --...... ,.-.. ·---· .,. .. ~·-.. - ... - .. _. . ....,. ....... .

Scr.een Size (mm.) lb. 4 2 l · o.5 0.25 0.125 thru per

Process

·•""""""····--· ...... ,.... .... . .·.· ., .............. , .. a .•. 125 .. - ..... , .... ·---bu .•.......

Percent Retained on Screen

Dry rolled 0!3 30 0 9 61.8 3.0 3,0 0.5 0.5 40.7

Recon.-22% 42 0 8 43,8 6,l 2.8 3.l l,4 0 27.2

Recon.-30% 41,4 35,5 8.6 9.3 4.9 0.3 0 24.5

Recon.-38% 50 0 2 38.7 5.9 4.2 l.O O O 22.3

Recon.-38%-1.-day 0.3 43.3 41.6 8.2 4.5 2~1 0 23 • .1 - ,,,,• <-• ·-··• • , 0 ,,,.,••-·-·· .~--.,,,,•,,S,,,..,..~-~ ... ,,,,•~,, ....... ,,,,,••·-·~<'•' N•>h"''"•..-•D~<,.P•••'-'•••,,· .................... ...,.. .'••·-~~ ....... , .... ,:,•••o,•'.C:,•,,•-....-,.,. • .,, •• ,. ,"•-•••~ ,•·• ,,.,._,,,,••W .. , , .. -,.-.,. ~'-'•4~---.... ·~

aParticle sizes Four 100 gm. samples of each grain were seived.

bTest weights.reported are the average of six determinations and are on a 90% dry matter basis. .

~ .....

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CHAPTER IV

RESULTS

Trial I

Feedlot Performance

Feedlot performance of the calve~ fed the three types

o.f .. .processed milo is sho.:wn in Table XXIV o Significant F

values were obtained for average daily gain (P<.05) and

feed/kg. empty body we .. ight._gain (P<.05). Comparison of

treatment means-indicated.the calves on reconstituted-ground

milo gained sign.ificant.ly (P<.05) faster than those on

ground-reconsti:tut.ed .milo. The ca_lves on the reconstituted­

ground milo ration required significantly (P<.05) less feed

per kg. of empty body weight gain than those on the ground­

reconstituted milo ration; the calves on the finely ground

milo ration were intermediate between the other two treat­

ments. The average daily intake of the total ration and

grain was almost identical for the three treatmentso

Although the differences in feed/kg. of gain of total ration

were not significant (P<.05) on a shrunk weight basis, the

calves on reconstituted-ground milo required 9.0% less

feed/kg. of gain than those on the finely ground treatment.

Net Energy

Net energy values of the three types of processeq mi'lo

42

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TABLE XXIV

TRIAL ls FEEDLOT PERFORMANCE {189 :PAYS)

Item

No. steers

Initial live. shrunk wt., kg. Final live shrunk wt. , kg o

Average da.i ly gain, kij. g Average dai.ly intake { total ration), kg. Average.:daily intake {grain),- kg. Feed/kg •. gain {total ration)• kg. Feed/kg, gain {grain), kg,

lni tial emp.ty body wt o , kg. F.inal emp.ty body wt • t kg. Average _daily EBW g_ain, kg. Feed/kg, EBW gain {total ration), kg.g Feed/kg .• EBW gain (grain) 9 kg.

aDry milo ground througa 3.18 mm •. screen.

Finelya Ground

12 185.90 386.501 2

1.02' 6,64 5.48 6.,34 5.23

174.90 366.50

1.011 2 6.64 • 5.47

b Recon.-Ground

12 188~80 408.402

1.14 6.63 5.51 5~77 4~79

177.70 387.60

1.111 6.02 5~00

c Ground-Recon.

10 189.90 385.801

1.00 6.70 5~50 6~56 5.39

178.70 367.80

1~002 6.80 -5.58

s-d x

0~04 0.14 0.11 0.19 0~14

o.o4 0.17 0.13

Fe

. f 7.40 0.04 0~08 3~86 2.56

5.92f 7.27 -6.39

b . . Dry milo was reconstituted -whole, s-tored 21 days, and g_round through 3 .18 mm. screen just prior to feeding.

cDry milo was ground through a 3.18 mm. screen, reconstituted, stored for 21 days and then fed,

dstandard error of treatment means.

eCalculated F value from analysis of variance.

fSignificant {P<~05) ~-gAny two means without a common number differ significantly {P<~05).

~ w

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44

are shown in Table XXV. Differences in net energy values

were nonsignificant (P>.05), The NEP value of finely ground

milo was 114,8 megcal,/100 kg, The NEP values oJ. ground­

reconstituted and reconstituted-ground milo were 3o0% and

13.9% greater, respectively, than the NEP of finely ground

mi loo

Carcass.Merit .. . t

Apparently treatment had no affect on carcass merit

for the criteria shown in Table XXVI. None of the F values

for any of the carcass traits approached significance

(P>.05).

Trial II

Feedlot Performance

Feedlot performance of the calves fed the seven types

of processed milo is shown in Table XXVII. Significant

(P<.05) F values were obtained for average daily intake of

both total ration and grain. Differences in total ration/kg.

of gain and grain/kg. of gain were also significant (P<.05).

Comparison of treatment means indicated. that the calves on

high moisture harvested-ground milo consumed significantly

less total ration and grain _.than those on the other six

treatments. The feed consumption of the calves on extra

finely ground, high moisture harvested-rolled and reconsti­

tuted-g:t'ound milo was very .similar. The feed intake of

coarsely rolled and finely ground milo was significantly

higher than that of the other five tre.atments. Feed/kg, of

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Net Energy Value

TABLE XXV

TRIAL Is _.NET -.ENERGY VALUES _QF PROCESSED MILO

Finely -Ground

Recon.­Ground

Ground­Recon.

s-a x

NEm+p of total rationC?

NEm+p of milod

~---.,--"!l!··;Megcal .• _/IlO.-k.g. ----------- .. 140.7 157.2 148,5 5,41

NEm of miloe

NE of milof p

158:.T

172.,-2_

114.8

a Standar.d. .. error. of treatment me.ans.. •.

170.5

196.Q

130,7

bCalculated F valua.fr.om anal}l-sis of variance,

c:Energy for gain. and maintenance . + intake._ of_ totaL ration,

16:0.·a

177.5

118.3

6,55 ..

8,15

d(Energy for l!;a.in. and main.te~.e.-... e~e.i:gy. attr.ibu.ted. tCl basal) + intake of milo.

eNE X L50, (l.50 = ratio of NE. to NEP on basis. of av. crude-fiber content). p m .

£Determined by dividing maintenance requirement and ene~gy gai~ed between miio and premix.on basis of ratio in ration (83% milo, 17% premix).

Fb

2~6.4

2,38

2.69

.,::­VI

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Item

No. steers , D - ' cr1C _ ressing 10 d Carcass grade R·b- . e 1.-eye area, sq., l.Do Fat thickness, in.,f Marblingg Cutability 9 %h

TABLE XXVI

TRIAL Is CARCASS MERIT

Finely Ground

12 611129 10.54 10024

Oo67 13.67 49.04

Recon.­Ground

J.2

61.72 10005 11.14 o.s1

13,50 48.,51

aStandard error of treatment means. bCalculated F value from analysis of; variance.

Ground­Recon.

10 61.89

9.87' 10. 66-0.80

14.00 48.,35

s-a x

Oo58 0.28 0.19 0.05 0.60 Oo22

cCalculated on basis of final live shrunk weight and chilled carcass weight. du.s.o~-A. grad.es converted to following numerical designationss .. h.igh prime-15, av. prime-14; low pr.ime-13 9 high choice-12, avo choice=ll, low choice=lO, high good-9, av~ good-8, low good-7.

eDetermined by measurements of ribeye tracings at the 12th rib~ fAverage of three measurements on ribeye tracingso

Fb

1.67 0.72 0.69 2,81 0.19 3.15

~Marb.ling...scorc0s, !=devoid minus to 30=abundant plus, with 3 scores per classification (minus, average, p1us).

hPercent of boneless trimmed retail cuts on carcass basis=5l.78 - 5o78 (fat thickness) - 4.,62 (% kidney fat)+ ,740 (ribeye area) - .0093 (chilled carcass wt,),

+' 0\

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TABLE XXVII

TRIAL 111 FEEDLOT PERFORV.At',CE

Item

No, steers No, days on feed

Initial live shrunk wt,, c/cg.

Final live shrunk wt,, kg. Average daily gain,. kg, Average daily intake (total ration), kg., Average. daiJ..y intake (grain, kg, c

Feed/kg, gain {total ration), kgc

Feed/kg, gain (grain), kg, c

Initial empty body wt,, kg, .Final empty body wt,, kg, Average daily EBw gain, kg, Feed/kg, EBW.gain (total ration), kg,

Feed/kg, EBW gain (grain), ki>;,

c

Coa·rsely Rolled

12 174

232,80

409.3')

1.01

7.654

6.314

7.604

6.274

218,70 394.50

1.01 7,61

6.28

aStandard error of treatment means,

bCalculated F value .from analysis of variance,

Finely. Ground

12 174

233.20

421. 70 l.06 7.614

6.294 7.123,4

5.883 •4

219., lO

405,10 . 1,06

7 ,20

5.95

Extra Finely Ground

11

175

234,80

412,00 0,99

6,652

5.492

6,643

5,482 •3

220.60 393, '.)')

0,99 6,82

5.63

cAriy tw'o values without a·cormnon number differ significantly (P<,05),

dSignificant {P<, 05),

Wil'I­Ground

ll

175

224.5')

401,60 0.99 6,081

5,031

6,472 •3

5.352 •3

211, 00 382.80

0.98 6.66 5,51

HMH­Rolled

12 174

232,80

440.60 1.17 6,872 •3

5,662 •3

5. 78 1

4,761

218.70 423,40

1.17 5,86 4,82

Recon,­Ground

12 174

229,60

411,60 l,02

6,802 •3

5,682 •3

6.603

5,513

215.70 393.80

1.02 6.73 5.62

Recon,­Rolled

11

173

233,80

444.90 l,19

7.113

5,923

5,921,2

4,921 •2

219,60 427,30

1.19. 6,01

5.00

a s­x

0,05 0,12

0.10 0,20

0.16

Fb

2,52 8,66d

8.53d 8,62d

a.sod

0.05 2.77 0.32 3. 77 0,27 3.66

+:­"

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gain for the high moisture harvested-rolled and reconsti­

tuted-rolled grains was significantly lower than that for

the dry processed grains. Feed/kg. of gain of high mois­

ture harvested-ground and reconstituted-ground milo was

significantly lower (P<.05) than on coarsely rolled milo.

48

Although differences in rate of gain were not signifi­

cant (P>,05), calves fed high moisture harvested-rolled and

reconstituted-rolled milo gained 10.3 and 12.0% faster,

respectively, than calves fed finely ground milo. Rates

of gain were very similar for the other treatments.

Net Energy

Calculated net energy values of the seven types of

processed milo are shown in Table XXVIII. Significant F

values (P<.05) were obtained· for NEm+p of total ration,

NEm+p of milo and NEP of milo. Comparison of treatment

means indicated that extra finely ground, high moisture

harvested-ground, high moisture harvested-rolled and

reconstituted-rolled milo were significantly (P<.05) higher

than coarsely rolled and finely ground milo for all three

net energy values. Also, reconstituted-ground milo was

significantly (P<.05) higher than coarsely rolled milo for

all three values.

Coarsely rolled milo produced an estimated NEP value

of 90.4 megcal./100 kg. Increases in NEP for the six other

processing methods, .compared to coarsely rolled milo, were

as follows: finely ground, 5.4%; reconstituted-ground,

24.8%; extra finely ground, 30.6%; reconstituted-rolled,

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Net Energy Value

TABLE XX.VIII

TRIAL Ilg NET ENERGY VALUES OF PROCESSED MILO

Coarsely Finely Extra HMH= HMH= Recono­Rolled Ground Finely Ground Rolled Ground

Ground

a Recon. = s-Rolled x

~~-~---~-~--~-~-~Megcal./100 kg.~~~-~~~~~~-·---~~~----NEm+p of total rationc 0 g 130061 13404192 151033, 157013 157 0 33 147 0 2293

.-. ?"'":, 156013 3o83

Fb

8.64h

NE of milod 9 g m+p 140 .. 61 145o01P2 165o63,' 172.53 173003 159072•3 170.63 4.58 's.74h

NEm of miloe 135.6 143.0 177.2 188.4 192.5 169.2

NEP of milof,g 90041 95.3 1'2 11s.13 125.63 128.33 112.82•3

aStandard error of treatment means. bCalculated F value from analysis of variance. cEnergy for gain and mai~tenance + intake of total ration a

191.6

127. 13 5.74

d(Energy for gain and maintenance - energy attributed to basal).;,, intake of milo.

7.59h

eNEP X L50p (1.50 = ratio of NEm to NEP on basis of av. crude fiber content)., fDetermined by dividing maintenance requirement and energy gained between milo and premix on basis of ratio in ration (83% milo, 17% premix).

gAny two values without a common number differ significantly (P<.05). hSignificant (P<.05).

.i;:-. \0

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41. 3%1 and high moisture harvested-rolled, t~L 9%.

Carcass Merit

The seven types of processed milo fed in this trial

produced carcasses that were not significantly (P<.05)

different for any of the criteria shown in Table XXIX.

Trial III

Feedlot Performance

50

Feedlot performance of the steers fed the five types

of processed milo is shown in Table XXXo Significant

(P<.05) F values were obtained for total ration/kg. of gain

and grain/kg. of gain. Comparison of treatment means

indicated that the feed efficiency of the reconstituted

30 and 38% milo was significantly improved over dry rolled,

22 and 38%-1-day milo.

Although differences in rate of gain were not signifi­

cant (P>o05), 22% milo produced gain 8.0% higher than dry

rolled milo. Rates of gain for the 30 0 38 and 38%-1-day

reconstituted milo were 2.4, 7.6 and 2.4% lower, respec­

tively0 than dry rolled milo. The heifers on 30 and 38%

milo consumed 15.0 and 19.1% less feed 9 respectively, but

because of similar gain, were 11.8 and 12.5% more efficient

in utilizing feed than the heifers on dry rolled milo.

Feed intake and feed efficiency were similar for the cattle

on dry rolled, 22 and 38%-1-day milo.

Feed efficiency values expressed as total ration or

grain/kg. of empty body weight gain produced results

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"""" Item

No. steers

Dressing %c d Carcass grade e Ribeye areaosq.in.f Fat thicknesso in. Marblingg h Cutabilityg %

TABLE XXIX

TRIAL Ils CARCASS MERIT

Coarsely Finely Rolled Ground

12 59.63

9.16 10.55

o.·62 12.08 49.52

12 59.29

9.67 10.45 0.72

13.,08 48.68

Extra HMH= HMH= Finely Ground Rolled Ground

11 59.07

9.00 10.68 0.63

11.91 49.58

11 61.43

9.45 10.32 0.60

12.55 49.48

12 60.22

9.75 11.21 0.67

13.42 49.28

aStandard error of treatment means. bCalculated F value from analysis of variance.

a Recon.- Recono= S= Ground Rolled x

12 59.12

9.25 10.25 0.64

12.17 49.31

11

59.94 9.82

10.70 0.72

14.64 49.87

2.26 0.31 0.17 o.59 0.39 0.41

cCalculated on basis of final live shrunk weight and chilled carcass weight.

Fb

0.91 l.07 3.63 0.67 0.62 0.74

du.s.D.A. carcass~grades converted to following numerical designationss high prime=l5p av. prime-14, low prime-13 0 high choice-12D av. choice-11 0 low choice ... 10, high good=9o av. good-8, low good-7. -

eDetermined by measurements of ribeye tracings at the 12th rib.

fAverage of three measurements on ribeye tracings. gMarbling scores, l=devoid minus to 30=abundant plus 0 with 3 scores per classification

(minus 9 average p plus) .. hPercent of boneless trimmed retail cuts on carcass basis=51.34 = 5.78 (fat thickness)

-4.62 (% kidney fat)+ .740 (ribeye area) - .0093 (chilled carcass wt.)o

VI I-'

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TABLE XXX

TRIAL Ills FEEDLOT PERFORMANCE (112 DAYS}

Item Dry Recon.- Recono= Recon .... Recon.- a Fb s ... Rolled 22% 30% 38% 38% ... l-day x

No. heifers 10 10 10 10 10 Initial live shrunk Wtoekg. 172010 170.30 173.00 166.00 172.80 Final live shrunk_wt. 9 kg. 299.40 308.20 297.80 289.60 296.70 Av. daily gain, kg. 1.14 1.23 1.11 l.05 1.11 0.05 1.65 . g 7 .63- 7.90 6.48 6.17 7.29 0.39 4.00 Av, daily intake» kg. Av daily intake 9 k§.h 6.412 6.621 2 5.43 5.18 6.162 0.33, 4.02 Feed/kg. gain» kg. ,g 6.782 6.511'2 5.98t 5.961 6.622 o. 16' 6.42c Feed/kg. gain 9 kg,e,h 5.70 5.46, 5.01 5.001 5.60 0.13 6.68c Initial empty body wt.,kg. 158.20 156.50 159.00 152.60 158.80 Final empty body wt.,kg. 287.10 295.80 286.50 278.00 283.00 Av. _daily EBW gain, kgo 1.152 1.242 1.131 l.071 1.112 0.05 1.61 Feed/kg. EBW gain, kg.~,g 6.70 6.44 5.80 5.88 6.62 0.11 15.26c Feed/kg·.- ··EBW ~gai:n, ·1cg;f,h ·- 5~622 - -5.402 ·

.astandard error of treatritent means. bCalculated F value from analysis .·of variance. cSignificant (P<.05). dSignificant (P< •. 01).

4.861 4;931-- ···· --·s;s92---·--·0;09- -16'; 11d

eAny 2 means without a conuhori'·'=number differ significantly (P<.05). fAny 2 means without a common numbe_r .differ significantly (P<.01). gTotal · ration., hGrain.'

VI N

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similar .to those previously discussed on a shrunk weight

basis.

Net Energy

The calculated net energy values of the five types of

processed milo are shown in Table XXXI. Significant F

values were obtained for NEm+p of the total ration and

NEm+p of the milo. Comparison of the treatment means

indicated that the NEm+p of the total ration and the milo

was significantly higher for 30 and 38% milo than for dry

rolled and 22% milo.

53

Dry rolled milo produced an estimated NEP value of

109.4 megcal./100 kg. Comparison of the other four proc­

essing methods with dry rolled indicated increases in NEP

as followss 38%-1-day milo, 6.4%; 22% milo 0 7.8%; 30% milo,

34.8%; and 38% milo, 39.3%.

Carcass Merit

The five types of processed milo fed in· this trial

produced carcasses that were not significantly different

for any of the criteria shown in Table XXXIl.

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-...._-_,

TABLE XXXI

TRIAL Ills NET ENERGY VALUES OF PROCESSED MILO

Net Energy Value Dry Recona"' Recon.= Recon.= Recon~--Rolled 22% 30% 38% 38%-1-day. _

c=,Q>c=,c:gcici;::r,e:::tc,cga;,C;:t~CQc;;aci;,Megcal .-·,100 kg. i=c;oc::ac,c=,CQC:SC:::,C:lltClmtO ______

NEm+p of total rationc 9 g 131.,31 135.81 157.12

NEm+p of milod 9 $ 137. 61 143.11 168.73

NEm of miloe 164.1 176.9 22103

NEP of milof 109.4 117.9 147.5

aStandard error of treatment means. bCalculated F value from analysis--o-f variance.

156~62

167.7203

228.6

152.4

cEnergy for gain and maintenance..;.. intake of total ration.

136. 61

143.61 ' 2

174.6

116.4

s-a x

5.04

6.04

9.71

d(Energy for gain and-maintenance - energy attributed to basal)+ intake of -milo.

eNEP X 1.50, (1.50 = ratio of NEm to NEP on basis of av. crude fiber content) .. fDeterm_ ined by dividing maintenance requirement and energr gained between milo and

premix on basis of ratio in ration (84% milo o- 16% premix)• _ gAny two values without a common number differ significantly (P<.05).

Fb

6.68

6.70

4.58

VI .,::,.

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TABLE XXXII

TRIAL 1118 CARCASS MERIT

Item

No. heifers Dressing %c d Carcass g.rade . e Ribeye area 0 sq. if .. Fat thickness 0 in. Marbling8 h Cutability, %

Dry Rolled

10 59.30

9.80 9.08 0.60

13.70 50.00

Recono ... Recon.-22% 30%

10 9 .59.39 59.42

9.90 9.30 9.46 9.59 0.63 0.56

14.30 13.80 50.22 50.41

~tandard error of treatment means. bCalculated F value from. analysis of variance.

Recon .... 38%

10 58.80 10.20 8.57 0.61

15.10 49.64

Recon~- s-a 38% ... l-day. x

10 59.21 0.640

9.80 0.130 9~08 0.415 0.61 0.064

13.60 0.406 50.24. 1.890

cCalculat.ed on basis of final live shrunk weight and chilled carcass weight. dUoS~D~A. grades converted to following numerical designationsg high-prime .. 15, av.

prime-14, low prime-l3, high choice-12, av. choice-11~ low choice ... 10, high good=9, av. good-So low good-7.

eDetermined by measurements of ribeye tracings at the 12th rib. fAverage of three measurements on ribeye tracings.

Fb

0.150 0.620 0.850 0.184 1.270 0.760

gMarbling scores, l~devoid minus to 30=abundant plus, with 3 scores per classification (minus, average~ plus).

hPerc:ent o'.f boneless trinuned .retail cuts on carcass basis=51. 78 ... 5. 78 (fat thickness) ... 4.62 (% kidney fat)+ .740 (ri}?eye area) - .0093 (chilled carcass wt.)~

VI VI

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CHAPTER V

DISCUSSION

The air-dry milo, both ground and rolled, averaged

8602% dry matter for the three trials. The moisture level

of the reconstituted treatments for all trials varied from

22.7% for. the reconstituted..,22% milo to 38% for the recon­

stituted-38% milo in Trial IIL The moisture level of the

reconstituted milo in Trial I averaged 30.3%, while in

Trial II the average was 26.1%. The high moisture harvested

milo fed in Trial II averaged 3005% moisture. It is inter­

esting to note that efficiency improved as moisture was

increased from 22.to 30% in Trial 111. Previous i!l vitro

work by Neuhaus (1968) indicated an increase in digestibility

as moisture increased to 38% .. The results of this feeding

trial showed no advantage for increasing the moisture level

from 30 to 38%i the available energy of the reconstituted-

38% milo was similar to that of reconstituted-30% milo.

The difference in results between the feeding trial and i!l

vitro trial could possibly be due to the lower gain of the

cattle on the reconstituted~38% milo compared to 30% milo

(Table XXX). The process of reconstituting milo to these

different moisture levels is of great practical significanceo

It is a relatively simple matter to raise the moisture level

56

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of milo to 22%, but raising it to 30% requires more elabo­

rate equipment and much longer exposure to water before or

during the _en.s.iling process. A further increase to 38%

moisture ac.tually .require.a .prolonged soaking of the grai,n

for approximately 10 hours .and would increase expense of

processing.

The result.s.of.Tria.l_II.indicated that particle size

is .an impo.r.tant .. f.actor .in .processing dry milo. The extra

. finely ground ... milo. improved. utilization_ appreciably over

finely g.round _.and .coarsely .rolled milo. Previous feeding

trial results .. comparing co.ar.sely ground to finely ground

milo showed.a .consistent advantage for the finer particles

if the grain had been .ground through a hammer mill .i( Baker

57

tt !:.!., 1955, Smith.tt .al., 1953 and Newsom, 1968). On the

other hand, rolled grain consisting of very large particles

is utilized. as effici.ently as grain which has been finely

ground through.a hammer.mill.(Smith and Parrish, 1953, Baker

tt il•o 1955 and Newsom, 1968). Apparently the rolling of

dry grain, as in the case of steamed grain, imparts some

characteristic to milo which.is beneficial to its utiliza­

tion. However,.the kernels must be broken; results of

Trial II showed that very coarse rolling, with many (25%)

kernels. unbroken, .. re.s.ulted ih decreased efficiency compared

to finely g.r.o.und .grain.,

All of the high moisture processing methods decreased

the density of milo .as compared to the respective dry control

in each trial. In Trial I, the ground-r~constituted and

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58

reconstituted-ground milo grains were 18,0 and 38,0% less

dense, respectively, than dry ground miloc There was consid­

erably less fine material in the reconstituted-ground milo

as compared to the other treatmentso In Trial 11 the extra

finely ground and the high moisture harvested-ground milo

grains were very similar in particle size 0 but the fluffy

nature of the high moisture grain was very evident as shown

by the test weight per bushel (density) of the two processed

grains 9 4008 and 31o3 lbe per bushel 9 respectivelyv for

extra finely ground and high moisture harvested=ground milo.

With the exception of ground=reconstituted milo in Trial I 0

feed efficiency improved as density was decreasedo This

same trend has been noted in steam flaking of milo (Hale

~ al. 0 1965) and steam flaking of corn (Matsushima and

Montgomery 0 1967) where a very flat flake was utilized more

efficiently than a thick flake. In most cases 0 a decrease

in density involves an increase in surface area which appar­

ently is conducive to greater utilization of the grain. In

the case of the 22 0 30 and 38% reconstituted milo in Trial

111 0 feed efficiency improved as moisture increased, with

little change in particle sizeo These results indicated

that density (pounds per bushel) is a more accurate indica­

tor of utilization of high moisture processed grains than is

particle sizeo Work by Neuhaus (1968) indicated that as

particle size of dry milo decreased in. vitro digestibility

increased 9 but particle size of high moisture milo had no

affect on in vitro digestihilit.yo

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59

Rolling of the high moisture processed grains markedly

improved feed efficiency as compared to grinding the same

high moisture processed grains in Trial llo This is consis­

tent with results reported by Newsom (1968)0 The marked

increase in rate of gain of the high moisture processed­

rolled milo was not observed in previous trials (Newsom 0

1968), and the increased rate of gain observed for highmois 00

ture processed~rolled grains in Trial II was not repeated in

Trial IIIo Also 9 the improvement in efficiency for the

reconstituted-rolled milo in Trial III was not as great as

in Trial IIo The roller mill used in Trial III was consid­

erably lighter in weight and therefore could not exert as

much pressure on the milo kernelo A decrease in roller

pressure might result in less surface area of the grain

being exposed and hence a lower digestibility of the grain.

Neuhaus (1968) showed that as roller spacing decreased (and

consequently pressure increased) the in vitro digestibility

of high moisture grain improved. Roller pressure is also

extremely important in increasing the digestibility of

steam .. flaked milo. Hale tt il• (1965) reported a flat rolled

product was distinctly superior to poorly rolled grain.

The results of Trial I indicated that reconstituting

milo in the ground form at a moisture level of approximately

30% did not improve utilization compared to finely ground

dry milo. Apparently the germination process is initiated

in reconstituted whole grain 0 which causes a reduction of

starch to simpler 0 more readily available carbohydrates.

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60

The enzymatic pathways would be destroyed by grinding before

reconstituting and this would prevent any beneficial effect

from the reconstituting processo Penic ~ al. (1968)

reported an improvement in feed efficiency of 11% for recon­

stituted whole milo as compared to dry ground milo; ground­

reconstituted milo did not improve utilization compared to

dry graino Since high moisture whole milo cannot be stored

in a trench silo without considerable spoilage 0 an airtight 9

upright structure is apparently needed for the successful

reconstituting of miloo

In Trial Il 9 it is interesting to note the similarity

in feeding value of the high moisture harvested and recon=

stituted milo. The ground "wet" grains and the rolled 0 wet"

grains improved feed efficiency approximately the same magni­

tude compared to dry finely ground milo. It is possible

that the carbohydrates in high moisture harvested milo are

intercepted before conversion from a more soluble form to

starch 0 and would compare to the carbohydrates resulting

from the partial hydrolysis of starch when limited germina­

tion occurs in reconstituted grain.

The int~ke and utilization of reconstituted milo are

at least partially dependent on moisture level and time of

storage 9 as shown by the results of Trial III. Feed consump­

tion decreased and feed efficiency improved as the moisture

was increased from 22 to 38% (milo was stored 21 days).

Since rate of gain was not significantly affected by mois­

ture level of milo 9 the heifers were apparently eating to a

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61

constant energy level. Energy was apparently available from

both 30 and 38% reconstituted milo to a similar degree.

Preliminary in vitro work showed considerable improve­

ment in dry matter disappearance for reconstituted milo

exposed to the atmosphere for one day. When this procedure

was incorporated into a feeding trial (Trial III)o the

improvement was not of the magnitude observed in the in.

vitro trialss In vitro digestibility determinations on

samples taken from the grain actually processed for the

feeding trial showed little benefit from reconstituti6n 9··,,

as was observed in the feeding trial. It is possible that

the laboratory technique of using a small sample of milo

allowed more complete germination than was possible when a

larger quantity was reconstituted for the feeding trial and

sprouting occurred only in the top 10 to 13 cm. of the grain

mass.

With the exception of ground-reconstituted milo in

Trial 1 9 all NEP values obtained for processed milo in

these trials ,were related to feed efficiency; as feed effi-

ciency improved, NEP values increased. The difference in

relationship of the NEP values and feed/kg. of gain in

Trial I was probably due to chance 0 as the values were very

similar. The mean NEP value for the finely ground milo

(Trials I and II) and dry rolled milo (Trial III) was 106.5

megcal./100 kg., which corresponds to a value of 108.0

megcal./100 kg. reported for Southwest milo by Lofgreen and

Garrett (1967). NEP values reported by Newsom (1968) for

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62

finely ground, reconstituted-rolled and reconstituted-ground

milo were 124.3, 152.5 and 124.3 megcalo/100 kg. 0 respective­

ly, as compared to 95.3, 127.7 and 112.8 megcal./100 kgo for

the same treatments in Trial II. The reason for the differ-

ence in NEP values is not apparent. Similar differences

were reported by Hall.!.! ll• (1968) 0 who pointed out that NE

determinations are subject to such variations.

The increased NEP of the high moisture processed grains

and the extra finely ground milo in Trial II, and of the

reconstituted-30 and -38% milo in Trial III, was probably

partly due to increased digestibility. An increase in milo

digestibility due to high moisture processing has been

observed by Buchanan-Smith.!.! !l• (1968) and McGinty and

Riggs (1967)0 Smith.!.! !lp (1949) reported an increase in

digestibility of dry matter, crude protein, ether extract

and nitrogen free extract when finely ground milo was com-

pared to coarsely ground miloo

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CHAPTER VI

SUMMARY

Three feeding trials were conducted to investigate the

factors influencing the utilization of high moisture proc­

essed milo. Coarse rolling, fine grinding and extra fine

grinding of dry milo were also compared in one feeding trial.

Evaluation was on the basis of feedlot performance, net

energy .and carcass merit.

Reconstituted-ground milo (reconstituted in whole form,

stored 21 days, then ground) significantly (P<.05) increased

rate of gain compared to ground-reconstituted milo (ground,

then reconstituted and stored. 21 days), and feed/kg. of gain

was markedly reduced by reconstituted-ground milo compared

to dry finely ground and ground-reconstituted milo. Feed

intake was almo.st identical for the three treatments in this

trialo Net energy for production (NEP) was not significant=

ly affected by processing method. It is apparent from the

results of this trial that milo must be reconstituted in the

whole form to benefit from the reconstituting process.

Grinding dry milo through a 1.59 mm. screen (extra fine

grinding) improved feed efficiency 6.7% over grinding milo

through a 3.18 mm. screen (fine grinding); the floury tex­

ture of the extra finely ground milo did not appreciably

63

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64

reduce intake. Coarsely rolled miloo which contained

approximately 25% unbroken kernels9 reduced feed efficiency

6.7% and decreased rate of gain slightly compared to finely

ground milo. The NEP value of extra finely ground milo was

significantly (P<.05) higher th~n that of finely ground and

coarsely rolled milo, indicating that particle size is an

important factor affecting energy utilization • ... l

With the exception of the ground=reconstituted milo 0

all high moisture processing methods improved utilization

of milo compared to fine grinding and dry rolling. Recon=

stituted-22% moisture milo increased consumption compared to

dry rolling; however 9 all other high moisture processing

methods reduced feed intake as compared to the respective

dry controls. Although not significant (P>a05) 9 rolling of

the high moisture processed grains increased rate of gain

10.5% as compared to grinding. High moisture harvesting and

reconstituting produced similar improvements in feed effi=

ciency. All high moisture methods significantly (P<.05)

increased NEP over coarsely rolled miloo

Milo reconstituted to moisture levels of 30 and 38%

significantly (P<.05) improved efficiency over dry rolled

milo. Feed/kg. of gain was reduced as the moisture level,

of reconstituted milo was increased from 22 to 30% for milo

stored 21 days, The storage of reconstituted milo for one

day was not sufficient to improve utilization appreciably.

Differences in NEP were not significant (P>.05) for the milo

grain reconstituted at different moisture levels 9 but NEP

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65

was increased 34.9 and 39,3%, respectively, for 30 and 38%

reconstituted milo compared to dry rolled milo. The improve­

ments in NEP were consistent with improvements in feed

efficiency.

Feed efficiency expressed as feed/kg. gain of live

shrunk weight was almost identical to feed efficiency

expressed as feed/kg. of empty body weight gain, indicating

that variable fill was not a problem under the conditions

imposed in this. study.

Carcass merit 9 as measured by dressing percent 9 carcass

grade, ribeye area 9 fat thickness and cutability, was not

significantly (P>.05) affected by processing method in these

trials.

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LITERATURE CITED

(1) Baker 0 Fo H., E. F. Smith, R. Fo Cox and D. Richardsono 1955, A comparison of rolled 0 coarsely ground and finely ground milo grain for fattening yearling heifers. Kan. Agro Exp. Sta. Circ, 320.

(2) Baker, F. S, 0 Jr. 1967, High moisture versus dry corn in steer finishing rations. Fla. Agr. Exp. Sta. Mimeo. Rpt. 67-6.

(3) Beeson, W. M. and T. W. Perry. 1958. The comparative feeding value of high moisture corn and low mois­ture corn with different feed add°itives for fatten­ing beef cattle, J. Animal Sci. 17s368·.

(4) Beesop, w. M., T. w. Perry and R. E. Honnold, 1956. High moisture ground ear corn vs. regular ground ear corn with and without antibiotics for fatten­ing steers. Ind, Agr. Exp. Sta. Mimeo, Ao H. 169.

(5) Beeson, w. M, 9 T. W. Perry and R. Eo Honnold, 1957. "Dynafac" and torula yeast as additives to high­and low-moisture ground ear corn for fattening heifers. Ind. Agr. Expo Sta. Mime.o. A. H. 204.

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(7) Branaman, G. A. and B. G. Harmon. 1960, The feeding value of ensiled high moisture ear and shelled corn, and of artifically dried corn for fattening cattle and effects of environment. Mich. State Univ. Cattle Feeders 0 Day Rpt. A, H. 61.

(8) Brethour, J. R. and W.W. Duitsman. 1961. Value of ensiled high-moisture sorghum grain (ground and unground) in a fattening ration for yearling steers. Kan. Agr. Exp. Sta. Circ. 382a8.

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66

i \ '\

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(10) Brethour, J. R, and W. w. Duitsman. 1963. Methods of processing sorghum grain for steer fattening rations·, High-moisture sorghum grain ensiled at two moisture levels compared with coarsely and finely ground dry grain, Kan. Agr. Expo Sta. Roundup Rpto 459s20,

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(19) Garrett, W. N, 0 G. P. Lofgreen and J, L, Hull. 1966. Steam pressure processing of grain for fattening cattle. Univ. of Calif. Dept. of An'imal Hus. · Calif, Feeders' Day Rpt.s25.

()

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(20) Garrett, Wo No, G, P. Lofgreen and J. L. Hull. 1967., Steam pressure processing of barley and milo. Univ. of Calif. Dept. of Animal Hus. Califo Feeders 9 Day Rpt.a27.

68

(21) Hale, W. H,, Bruce Taylor 9 W. Jo Saba and Kenneth Palmer. 1964. The effects of steam processing milo and barley and the use of various levels of fat with steam processed milo and barley in high­concentrate steer fattening ratlons. Ariz. Agr. Exp. Sta. 1965 Cattle Feeders 0 Day Rpt.al6.

(22) Hale, w. H., Bruce Taylor, Brent Theurero W. J. Saba, Howard Fredericks and E. L, Brown. 1965. The effect of steam'processing milo and barley for high concentrate steer fattening rations. Ariz. Agro Exp. Sta. 1965 Cattle Feeders 0 Day Rpt.s46.

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(24) Hentges, J. F., Jr., J. W. Carpenter and A. z. Palmer. 1962. Comparing four corn rations for fattening beef. Fla. Agr. Exp. Sta. Research Rpt. Vol, 7 -No. 3.

(25) Hodge, D. E., M. P. Plumlee and W. M. Beeson. 1959. Digestible energy determinations of high and regular moisture corn and cob meal, corn cobs and soy mill feed using identical twin beef calves. J. Animal Sci. 18s1527o (Abstr.).

(26) Hubberto Farris, Jr., Bruce Taylor, W. H. Hale, W. C. Carey, Jr. and E. B, Stanley. 1962. Barley vs. milo in Arizona feedlot rations. Ariz. Agr. Exp. Sta. 1962 Cattle Feeders 0 Day Rpt. s3l.

(27) Johnsono D. E., J-. K. Matsushima and K. L. Knox. 1967. Utilization of flaked vs. cracked corn by steers. J, Animal Sci. 26s876. (Abstr.).

(28) Larson, W. M,o L.B. Embry, R. M. Luther, L. J. Nygaard and D. H. Bushman. 1966. Dry and "reconstituted" high-moisture corn grain fed with and without roughage once and twice daily to finishing beef steers. s. Dak. Agr. Exp. Sta. A. s. Series 67-7.

(29) Larson, W. M., L. B, Embry Dry and high-moisture daily to beef steers. (Abstr.).

and L. J. Nygaard. 1966. corn fed once and twice J. Animal Sci. 2511245.

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(30) Little, c. O., N. W. Bradley and B. M. Jones, Jra 1962. Ground, flaked and pelleted corn for fattening beef steers. Ky. Agr. Exp. Sta. Progress Rpt. 116220.

(31) Lofgreen 9 G. P. and W. N. Garrett. 1967. Net energy requirements and feed values for growing and finishing cattle. Calif. Cattle Feeders 9 Annual Meeting, Univ. of Califo

(32) Matsushima, J. K. 1966. Flaking of grain for fattening cattle. Proc. of 17th Mont. Nutr. Conf.

(33) Matsushima 9 Jo Ko, D. E. Johnson 9 M. We Heeney and B. Ao Weichenthal. 1965. Greater feed efficiency from flaked corn. Colo. Farm and Home Research.

(34) Matsushima, Jo K. and Ro Lo Montgomeryo 1967. The thick and thin of flaked corn. Colo. Farm and Home Research. Vol. 179 No. 2.

{35) Matsushima, J. K. and Norris J. Stenquist. 1968. Corn& reconstituted 9 fine ground, dry rolled, ensiled and flaked. Colo. Farm and Home Research. Vol. 18, No. 2.

(36) McCroskey 9 J. Eo 9 Lo So Pope and Kenneth Urbano 1960. Further studies on pelleting rations for steers. Okla. Agr. Expo Sta. Bul. MP-57s96.

(37) McGinty 9 D. D., P. Penic and E. Jo Bowers. Moist grain for finishing beef cattleo Sci. 27sll70.

1968. J. Animal

(38) McGinty, D. D. and J. K. Riggs. 1967. Moist sorghum grains for finishing cattle. Tex. Agr. Exp. Sta. Dept, Tech. Rpt. No. 10s94.

(39) Mehen, Stephen, w. H. Hale 9 Brent Theurer and o. M. Little. 1966. Digestibility of dry-rolled, fine ground, steam-processed and pressure-cooked milo by steers. Ariz. Agr. Exp. Sta. 1966 Cattle Feeders 0 Day Rpt.s43.

(40) Morrison, Frank B. 1959. Feeds !!'.!£ Feeding (22nd ed.),, The Morrison Publishing Co., Clinton, Iowa.

(41) Murphey, c. E., D. K. Hallett, W. E. Tyler and J. c. Pierce, Jr. 1960. Estimating yields of retail cuts from beef carcasses. 62nd Meeting of the Amo Soca of Animal Prodo 9 Chicago.

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(42) Neuhaus 0 Vincent Do 1968. Factors affecting the in vitro digestibility of moist sorghum grain. Okla. Agro Exp. Sta. M. S. Thesis.

(43) Neuhaus, Vincent D. 1968. Unpublished data. Okla. Agr. Expo Sta.

(44) Newland, Ho W. 9 Go Ao Branaman and Richard Wo Rice. 1960. Heated corn and pelleted hay for fattening cattle. Mich. State Univ. Cattle Feeders 8 Day Rpt o A. H. 58 o

(45) Newsom 0 James Ro 1968. The effect of milo processing method on feedlot performance, carcass merit and net energy. Okla. Agr. Exp. Sta. Mo So Thesiso

(46) Newsom·, James Ro, Robert Totusek 9 Robert Renbarger 9

E. c. Nelson 9 Larry Franks 0 Vincent Neuhaus and Willie Basler. 1968. Methods of processing milo for fattening cattle. Okla. Agro Exp. Sta. Bulo MP-80s47o .

(47) Parrett, No A. and J. Ko Riggs. 1966. Dry, reconsti­tuted and early harvested grain sorghum for finishing cattle. Cons. Prog. Rpt. Noo 2423. Tex. Agr. Exp. Sta.

(48) Parrott, Cal, Steve Mehen 9 W • .H. Hale, Morgan Little and Brent Theurer. 1967. Digestibility of dry­rolled and steam-processed barley, Ariz. Agr. Exp. Sta. 1967 Cattle Feeders 0 Day Rpt.a56.

(49) Penic 0 Peter, P. To Marion, J. Ko Riggs and D. Do McGinty. 1968. Physical form as a factor in reconstituting sorghum grain for beef cattle. Tex.Agr. Expo Sta. Cons. Prog. Rpt. No" 2557-2567s26.

(50) Perryo T. W.o w. M. Beeson and D. D. Cope. 1960. The value of an implanted tranquilizer and of supple­mental enzymes when fed with either high-moisture ensiled shelled corn or regular shelled corn to fattening beef steers. Ind. Agr. Exp. Sta. Mimeo. AS-276.

(51) Perry 9 To W., C.H. Nickel 9 Donald Webb and Wo M. Beeson. 1961. The comparative value of pelleted rations containing 20%, 40% or 60% hay for fatten­ing beef steers. Ind. Agr. Exp. Sta. Mimeo. AS-298.

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(52) Pope 9 L, S., R, F. Hendrickson, Lowell Walters p George Waller and Wo D. Campbell. 1958. Effect of rolling vs. pelleting milo 9 previous implantation9 and certain feed additives on the feedlot perfor­mance of steer and heifer calves., Okla. Agr. Expo Sta. Bul. MP<m5lsll0.

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(54) Pope 9 L. S., George Waller 9 George Odell and Wa De Campbello 1962. Methods of processing milo for fattening steer calves. Okla. Agr. Exp. Sta. Bul. MP-67sll3o

(55) Pope, L. S. 9 L. E. Walters 9 George Waller and W. Do Campbell. 1959. Rolled vs. pelleted milo and certain feed additives for fattening steer calves. Okla. Agr. Exp. Sta. Bulo MP-55sl19.

(56) Pope 9 Lo So 9 Lowell Walters 9 George Waller 9 Jr. and W. Do Campbell. 1960. Effect of pelleting and steam rolling milo 9 with and without enzymes 9 for fattening steer calves. Okla. Agro Exp. Sta. Bulo MP-57sl34.

(57) Richardson 0 D. 9 E. Fo Smith 9 B. Ao Koch 9 F. W. Boren and W. s. Tsien. 1960. Rolled vs. finely ground pelleted sorghum grain in cattle rations. Kan. Agro Exp. Sta, Circ. 378.

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(59) Smith 0 Eo F. and Do B. Parrish. 1953. A comparison of rolled 0 coarsely ground and finely ground milo grain for fattening yearling steers. Kan. Agr. Expo Sta .. Circ. 297 o

(60) Smitho Eo F .. 0 D. B. Parrish and Ao G. Pickett. 19490 Effect of grinding on the nutritive value of grain sorghums for fattening steer calves. Kan. Agro Exp. Stao Circ. 250s37o

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(62) Steel, .R. G, and J. H, Torrie. Procedures of Statisticso New Yorke

1960. Principles s.E£, McGraw-Hill Book Co.v

72

(63) Tannerp James Eo 9 Jack A. Richey 9 Richard L. Willham and Joe Vo Whiternano 19670 Differences in growth pattern and carcass development of Angus bulls 9

steers and heiferso Okla. Agr. Exp., Sta. M. P .. 79831.

(64) Taylor 9 Bruce, Farris Hubbert, Jr. 9 W. Ho Halev W. Cci Careyu Jr. and Eo Bo Stanley. 19610 Barley VSo

milo in Arizona feedlot rations. 1961 Cattle Feeders 0 Day Rpt.a29.

(65) Taylor 9 Bruce, Farris Hubbert, Jr. 9 C. Bo Roubicek 9

R. Eo Taylor 0 Eo B. Stanley and Eo H. Hussmann. 1960., Steam-rolled vso dry-rolled milo and barleyo Arizo Agr. Expo Sta. 1960 Cattle Feeders 0 Day Rpto824.

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Franks, Willie Basler and Robert Methods of processing milo for Oklao Agro Expo Sta. Bul.

(68) Totusek 9 Robert 0 Dwight Stephens and Lowell Walters. 19640 Improving the utilization of milo for fattening calves~ value of fine grinding and supplemental vitamin A. Okla, Agr. Exp, Sta. But. MP-74 s 63.

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VITA~

Dennis Ralph White

Candidate for the Degree of

Master of Science

Thesiss FEEDLOT PERFORMANCE, NET ENERGY AND CARCASS MERIT AS AFFECTED BY HIGH MOISTURE VS. DRY METHODS OF PROCESSING MILO

Major Fields Animal Science

Biographical s

Personal Datas Born in Chickasha 9 Oklahoma 9 July 19 0

1942 9 the son of Mr, and Mrs. Ralph H. White; married to Norm.a L. Ganna the father of one son, Gregory Lynn White.

Educations Graduated from Ninnekah High School 9

Ninnekah 0 Oklahoma 0 in May, 1960g received the Bachelor of Science degree from Oklahoma State University in May, 1964, with a major in Animal Scienceo

Professional Experiences Reared on a diversified farm in southwest Oklahoma. Worked in Provision Depart• ment9 Wilson and Company, Inc. 0 1964-68. Graduate Assistant at Oklahoma State University, 1968=69,

Professional Organizationss Phi Kappa Phi, Alpha Zeta and Block and Bridle Club.