fermenters & bioreactors

In-situ Sterilizable Glass Fermenters – The First in India

adept, as the name stands for, we offer you a

proficient system for your bioprocess needs.

In line with the initiative of Government of India “MAKE IN INDIA”, we have taken a step to provide an innovative “insitu sterilizable design glass

bioreactors” to the world to reduce the risks and hassles faced by the currently used “autoclavable bioreactors”. We have taken care of the fact that Migration to this newly available design & technology may take a while and hence we offer the standard Autoclavable versions of bioreactors also. Apart from these we do offer Airlift fermentors to meet the customers

working with samples of high oxygen demand with no shear forces. The air-lift systems too are in-situ sterilizable to avoid carrying such large & heavy vessels to big autoclaves. With an experienced team of around 20 years in bioprocess industry, we proposed to have a much simple to use yet sophisticated bioreactor system which got materialized by 2011 (as the first prototype). After the introduction of this in-situ sterilizable glass bioreactors, Scientists get the advantage of risk free sterilization of their medium in the complete scale ranging from 3 Liter to 15 Liter working Volume Systems.

Complete Cultivation Units

We offer a complete plug and play type system with complete accessories for your cultivation needs.

The complete system includes the cultivation vessel, sensors, pumps, tubing and controller with data

logging software, spare part kits, chiller unit for temperature regulation and a suitable air

compressor unit for air supply to the bioreactor unit.

Unmatchable Quality

Every system is made of the finest quality materials and highest possible standards. For

instance the vessel head plate is made of high quality 316L grade Stainless Steel, glass vessel

made of toughened borosilicate glass. We offer a 3 year complete warranty on our vessel

components.

Exceptional application support

Our experienced and highly trained application engineers are readily available to support

you. Hands-on training is also available.

Maximum Flexibility

All the system provided by us is exceptionally flexible to accommodate every need of the

user, may it be having an exit gas O2/ CO2 analyzer or Methanol sensor or online viable

biomass monitoring system or online OD monitoring system or an external Solid State LED

based illumination system for phototrophic cultivation, we have solution for everything!

Data Acquisition

ON line data acquisition from fermentor for all the parameters like Temp, pH, DO, RPM

(Stirrer Speed) is stored in this unit and is directly visualized in computer real time with trend graph

of all parameter like temp, pH, DO, RPM vs time. Facility to review all old data is possible with few

clicks of the buttons. This Unit can be kept anywhere (max 200mts away) from fermentor via cable

and it has its own display unit (LCD) and continuously shows the condition of fermentor parameters.

The Data Logging facility is also available on a tablet and there is a possibility to interface it with your

GSM device for remote access to data, maybe on a cell phone (OPTION).

Out-of-box Design

Glass Insitu sterilizable bioreactors :- adept-i

The unique design of the fermentor system eliminates the need to moving the reactor to a

autoclave for sterilization. It has an inbuilt heating system which takes the vessel’s

temperature to 121 °C at a pressure of 15 psi ( Similar to the temperature and pressure

conditions inside an autoclave ). The parameters which can be measured and controlled

may be pH, DO, Temperature, agitation, level, pressure, foam etc ( as standard ).

Head & Bottom Plate

The head & bottom plates are made of high quality stainless steel available and designed to

accommodate a number of useful accessories without compromising the sterility. It has

numerous threaded ports to provide high degree of flexibility for sensors, sample tube etc

and provided with blind stoppers for unused ports.

Vessel

Size

Working

Volumes

Top Plate Ports Bottom Plate Ports Total Ports

42mm 30mm 18mm 10mm 25mm 10mm

4L 1.5–3.0L - 1 10 1 3 3 18

7L 2.0 – 5.5L - 1 10 1 3 3 18

10L 2.0-7.5L 1 - 11 1 4 3 20

15L 4.0-12.0L 1 - 11 1 4 3 20

20L 6.0 – 16 L 1 - 11 1 4 3 20

Air lift Fermentor:- adept-ALF

ADEPT-ALF has the following Advantages:

Its Simple design with no moving parts or agitator accounts to less maintenance, less risk of handling

errors. The Airlift bioreactor doesn’t need the heating plate to control the temperature because the

Draught-Tube which is inside the bioreactor is designed to serve as internal heat exchanger.

SS 316 L Components with Industry Standard Finish

Autoclavable bioreactors:- adept-AF

Our valuable experience has enabled us to deliver the most innovative, reliable and cost effective

solutions. We offer a comprehensive range of autoclavable that are manufactured with high level of

precision. Our products are manufactured with strict conformity with international quality standards

and undergo rigorous test before delivery.

We offer a wide range of glass (dished bottom) vessels made of borosilicate glass with volumes starting

from 1L to 20L. The bioreactor headplate is made of high quality SS316L and has a mirror finish look to

suit the lab etiquette. The complete system is very simple to set-up and easy to handle.

Vessel

Size

Working

volumes

Head Plate Ports Total

Ports 6mm 10mm 12mm 27mm 69mm M18x1.5 G3/4” M30 x 1

1L 0.3-0.9L 3 6 - - - 5 1 1 6

2L 0.5-1.7L 3 6 - - - 5 1 1 16

3L 0.5-2.7L 3 6 2 - - 5 1 1 18

5L 0.9-3.4L - 10 2 - - 5 1 1 19

7L 1.5-5.4L - 10 2 - - 5 1 1 19

15L 3.0-12.0L - 10 2 6 1 - - - 19

20L 3.0-16.0L - 10 2 6 1 - - - 19

Wide range of Options to best suit your application

Intelligent pH/ DO Probes for your bioprocess

ADEPT uses the widely used Bradley James probes for pH, DO.

Exit O2/CO2 gas analyzers

ADEPT when offered with exit gas analyzer will offer a sophisticated CO2 and O2 gas

measurement for consistent and reliable information to have a greater understanding of your

cells and processes. For more information kindly contact us.

Methanol monitors

We offer a stand-alone methanol sensor that provides the reliable & online control of

methanol concentrations in the cultures of methanolotrophic organisms allowing fast and

efficient optimization of recombinant protein production. For more information kindly contact

us.

Online cell density monitoring system

Knowing the health of culture will always be of interest to the users. We offer the cell density

monitoring system based on the capacitance principle and spectrometric principle to provide

the detailed events of the growing cultures inside the bioreactor vessel.

Illumination unit

ADEPT offers a valuable add on to its users working on phototrophic cultures by providing the Solid

State Light based Illumination light bank with control of intensities and to provide the day night cycle

stimulation to the growing cultures.

Different Modes of Cultivation

ADEPT offers the complete range of accessories for batch, fed-batch or continuous modes of cultivation

as per the customer’s requirement.

Technical Specifications

Vessel Design Glass

Controller Control Station

Agitation

Drive Direct drive, Mechanical coupled

Maximum Stirrer

Speed in RPM 50- 1000

Impellers Rushton or Marine Impeller with outside diameters 45mm, 60 mm

75 mm

Gas Sparger Porous sparger or L-type sparger

Gas Overlay Yes

Exhaust Gas Air outlet pipe or Water cooled exhaust gas condenser

Heating Heating Finger & U-tube heat Exchanger for cold water circulation

Additions Triple or single inlet ports and optional micro liquid injectors

pH

Sensor 12 mm gel filled pH sensor

Range 2-12 pH

Control via acid pump or CO2 gas (rotameter) in combination with alkali

pump

DO

Sensor 12 mm classic Galvanic/Polarographic DO2 sensor

Range 0 – 100%

Control

Via standard air purging (Rotameter) or optional with a

combination of N2, CO2, O2 (Rotameter). Can be cascaded with

agitation or other external loops.

Aeration

Temperature

Sensor Pt 100 sensor

Range 5 °C above coolant temperature to 80 °C via PID control

Control

Pressure

Sensor Digital Peizo electric sensor

Range 0-50psi

Control Pressure Safety cut off of Heater vs Pressure

Other Sensors Foam/ Level sensors, Methanol Sensor, Viable Biomass sensor, OD

sensor

Actuators Pumps 3 fixed pumps(upto 6)

Rotameters/ MFC 2Standard (upto 4)/ Upto 4 MFCs

Utilities Water & Gas Water: 10 PSIG maximum, 50 µ filtration. Gas: 10 PSIG maximum

Electric

Requirements 208 - 230 V 50/60 Hz

Net Weight Control Unit

Dimensions

(wxdxh) Main Control Unit