figure 1 : principle of pet scan imaging
DESCRIPTION
Preload and afterload with L -amino acids to enhance the differentiation between tumor and inflammation by labelled amino acids PET imaging. Laïque Salma [1] , Egrise Dominique [1] , Lemaire Christian [2] , Monclus Michel [1] , Schmitz Frédéric [1] , Luxen André [2] , Goldman Serge [1] - PowerPoint PPT PresentationTRANSCRIPT
Preload and afterload with Preload and afterload with LL-amino acids to -amino acids to enhance the differentiation between tumor and enhance the differentiation between tumor and
inflammation by labelled amino acids PET imaginginflammation by labelled amino acids PET imaging
Aim :Aim : Among labelled amino-acids, FET and FT are transported across the cellular membrane by the exchanger l–system. In vitro utilisation of FET and FT, after preload or prior to postload of non-radioactive L-amino-acids, was evaluated to measure the effects of intra- and extra cellular amino-acid content on the differential tracers uptake in tumor (ROS 17/2.8) and inflammatory cells (human leukocytes).
Laïque Salma [1], Egrise Dominique [1], Lemaire Christian [2], Monclus Michel [1], Schmitz Frédéric [1], Luxen André [2], Goldman Serge [1]
[1] PET/Biomedical Cyclotron Unit, ULB, Hopital Erasme, Route de Lennik 808, 1070 Anderlecht, BELGIUM[2] Cyclotron Research Center, ULG, B 30 Building, 4000 Liège, BELGIUM
Figure 1 : principle of PET Scan imaging
FET : O-(2-[18F] fluoroethyl)-L-Tyrosine : not incorporated into proteins
O
NH2
O
OH
F
O
NH2
OH
OH
F
FT : 2-[18F] fluoro-L-Tyrosine : incorporated into proteins
Figure 2 : FDG-PET revealing, an inflammatory lesion in the
mediastinum, in a patient suffering from sarcoidosis
FET Chemistry (1):FET Chemistry (1): FT Chemistry (2) FT Chemistry (2) ::
Figure 5 : preload effect = ratio between the tracer uptake into preloaded cells and control cells
ConclusionsConclusions : : L–tyrosine preload, prior to FET administration, can help in the differentiation between tumours and inflammatory lesions : see figure 6
Figure 6 : tracer uptake = % of loaded activity
References :References :(1) Wester HJ, Herz M, Weber W, Heiss P, Senekowitsh-Schmidtke R, Schwaiger M, Stöcklin G.Synthesis and radiopharmacology of O-(2-[18F]fluoroethyl)-L-tyrosine for tumor imaging. The Journal ofNuclear Medecine (1999), 40 (1) : 205-212.(2) Lemaire C, Gillet S, Guillouet S, Plenevaux A, Aerts J, Luxen A.Highly enantioselective synthesis of no-carrier-added 6-[18F]fluoro-L-dopa by chiral phase transferalkylation. European Journal of Organic Chemistry (2004), (13) : 2899-2904. (3) Ooi Takashi, Takeuchi Mifune, Kameda Minoru, Maruoka Keiji. Practical catalytic enantioselective synthesis of a -dialkyl--amino acids by chiral phase-transfercatalysis. Journal of the American Chemical Society (2000), 122 (21) : 5228-5229.
Table 1 : differenciation between ROS 17/2.8 and leukocytes = ratio between FET content in ROS 17/2.8 and in the inflammatory cells
Figure 3 : preload effect = ratio between the tracer uptake into preloaded cells and control cells
Figure 4 : preload effect = ratio between the tracer uptake into preloaded cells and control cells
L-tyrosine preload prior to FET administration on ROS 17/2.8 and leukocytes : figure 3
FET Biology :FET Biology :
L-methionine preload prior to FET administration on ROS 17/2.8 and leukocytes : figure 4
L-phenylalanine load after FET administration on ROS 17/2.8 and leukocytes : table 1
Contact : : [email protected] : 0032-2-555.47.11
FT Biology : FT Biology : L-tyrosine preload prior to FT administration on ROS 17/2.8 : figure 5