50 samples 100 samples 150 samples 180 samples0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

FiveFourThreeTwo

Prob

abili

ty

Two Three Four Five0

0.05

0.1

0.15

0.2

0.25

0.3

50 samples

100 samples

150 samples

180 samples

All samples

No of optimal number of clusters

Aver

age

silho

uett

e va

lue

Figure S1

A. B.

Figure S2

Bacteroides

prevotella

Ruminococcus

Figure S3

1

2

3

45

6

Figure S4

0 0.5 1 1.5 2 2.5 3 3.50

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

Shannon diversity

Silh

ouett

e va

lues

Fig S5

Pearson correlation=-0.96

left retroauricular crease

Figure S6

womenmenSt. LouisHouston

BMI>=30

BMI<25NA

not hispanic/latino/spanishHispanic/latino/spanish

25 <= BMI <30

right retroauricular crease

right antecubital fossa

Figure S6.

left antecubital fossa

Keratinized gingiva

Posterior fornix

PH>0.45

Figure S6.

Vaginal introitus

mid vagina

Figure S6.

Buccal mucosa Hard palate

Figure S6.

Tongue dorsum stool

Figure S6.

Anterior nares Subgingival plaque

Figure S6.

Supragingival plaque throat

Figure S6.

palatine tonsil saliva

Figure S6.

Propionibacterium CorynebacteriumStaphylococcus

Figure S7.

A.

Propionibacterium CoryneacteriumStaphylococcus

Propionibacterium CorynebacteriumStaphylococcus

B.

C.

*

**

Rela

tive

abun

danc

e %

Rela

tive

abun

danc

e %

Rela

tive

abun

danc

e %

Left retroauricular crease Left antecubital fossa

anterior nares

*

E.

male femalemale female

D

* *

Figure S7.

Rela

tive

abun

danc

e %

Rela

tive

abun

danc

e %

Moraxella in anterior nares Prevotella in stool

23

2

Lactobacillus (25 visit 1) Anaerobic group (4 visit 1)

22

Figure S8A.

vaginal introitus

16

00

Ralstonia( 3 visit 1) Staphylococcus (0 visit 1)

4

Propionibacterium( 24 visit 1)Mixture (6 visit 1)

6

2

1

11

1

1

Figure S8B antecubital fossa