figures. fig. 1 schematic of a lab-built drop-type chemiluminescence (dcl) system
TRANSCRIPT
Figures
Fig. 1 Schematic of a lab-built drop-type chemiluminescence (DCL) system
NH2
NH 2
H2
N
NH
2
NH
2
NH
2
NH2
NH 2
H2
N
NH
2
NH
2
NH
2
Amide bond
NH2
NH 2
H2N
NH
2
NH
2
NH
2
Amide bond
BSA
BSA
BSA
BSA
BSA
BSA
HRP
NH2
N H2
H2
N
NH 2
NH2
NH
2Amide bond
BSA BSA
BSA
HRP
NH2
NH 2
H2N
NH
2
NH
2
NH
2
BSA
BSA
BSA
BSA
Amide bond
EDC/NHS
Fig.2 Sample treatment procedure for DCL measurement to determine enrofloxacin and its
metabolite in chicken meat
BSA
Fig.3 EF-TEM image of synthesized and surface modified magnetic nanoparticles (amine-functionalized SiO2@Fe3O4 nanoparticles); core 10.5±1.7 nm, shell 16.3±1.2nm
Fig.4 (a) FT-IR spectrum of amine functionalized SiO2@Fe3O4 nanoparticles; silica and NH2 group functionalized MNP
Fig.4 (b) FT-IR spectrum of amine functionalized SiO2@Fe3O4 nanoparticles; enrofloxacin attached MNP.
Supplementary DATA
YYY Y Y
Y YY
YYY YYYY YY
Enrofloxacin
Y
Primary antibody
Y
Y
Secondary antibody (HRP)
Substrate(TMB)
S-fig. 1. Experimental procedure for ELISA
S-fig. 2. Calibration curve of Enrofloxacin in chicken sample
0 1 2 3 4 5 6 7 80
500
1000
1500
2000
2500
Linear Regression for Data1_B:Y = A + B * X
Parameter Value Error------------------------------------------------------------A 64.90005 44.12143B 300.06453 12.42628------------------------------------------------------------
R SD N P------------------------------------------------------------0.99574 77.95663 7 <0.0001------------------------------------------------------------
Inte
nsi
ty (
cou
nts
)
Concentration (ng/mL)
Enrofloxacin Linear fit Enrofloxacin in 1xPBS Luminol 10mM
H2O2 100mM
Boric acid 100mM
P-Iodophenol 1.5mM
pH 9.5
LOD (pg/mL) R2 RSD (%)
13.92 0.991 <11.4
S-fig 3. Calibration curve of Ciprofloxacin in chicken sample
Luminol 10mM
H2O2 100mM
Boric acid 100mM
P-Iodophenol 1.5mM
pH 9.5
LOD (pg/mL) R2 RSD (%)
7.26 0.994 <8.31
0 1 2 3 4 5 6 7 80
500
1000
1500
2000
2500
Linear Regression for Data3_B:Y = A + B * X
Parameter Value Error------------------------------------------------------------A 45.49055 37.47924B 308.89804 10.55559------------------------------------------------------------
R SD N P------------------------------------------------------------0.99709 66.22077 7 <0.0001------------------------------------------------------------
Inte
nsity
(co
unts
)
Concentration (ng/mL)
Ciprofloxacin Linear fit Ciprofloxacin in 1xPBS
S-fig. 4. Calibration curves of Enrofloxacin and Ciprofloxacin in chicken using ELISA
0.1 1 100
10
20
30
40
50
60
70
80
90
100
Linear Fit for Data1_B on linearized scales.yscale(Y) = A + B * xscale(X)where scale() is the current axis scale function.
Parameter Value Error------------------------------------------------------------A 77.48886 2.36547B -35.20315 3.6113------------------------------------------------------------
R SD N P-------------------------------------------------------------0.98964 2.63491 4 0.01036------------------------------------------------------------
Re
lativ
e a
bso
rba
nce
(%
)
Concentration (ng/mL)
Enrofloxacin Linear fit Enrofloxacin w/ ELISA
LOD (ng/mL) R2 RSD
(%)
1 0.979 <39.24
0.1 1 100
10
20
30
40
50
60
70
80
90
100
Linear Fit for Data1_B on linearized scales.yscale(Y) = A + B * xscale(X)where scale() is the current axis scale function.
Parameter Value Error------------------------------------------------------------A 54.74166 1.73488B -33.06515 2.64859------------------------------------------------------------
R SD N P-------------------------------------------------------------0.99364 1.93249 4 0.00636------------------------------------------------------------
Rel
ativ
e ab
sorb
ance
(%
)
Concentration (ng/mL)
Ciprofloxacin Linear fit Ciprofloxacin w/ ELISA
LOD (ng/mL)
R2 RSD (%)
1 0.987 <24.96