Chen_Fig.S1

-6

-1

4

Log 2

(Fol

d C

hang

e)

-11

-6

-1

Log 2

(Fol

d C

hang

e)

Bub1b

Cdc20

Cdc27

Cdc40

Dcp1a

Lin7c mos

pcm1

pum2

suz1

2

-5

-1

3

MI MIIGV

Log 2

(Fo

ld C

hang

e)

Polysome microarray

Polysome qPCR

RNP qPCR

Total qPCR

0

5

1 0

1 5

Fold

Cha

nge

Bmp6

Dppa3

Hnrnph

3

Syvn1

Ywhae

-5

-1

Log 2

(Fol

d C

hang

e)

0

1

2

Fold

Cha

nge

Polysome qPCR

Polysome microarray

A

B

Chen_Fig.S2

-9

-5

-1

Log 2

(Fol

d C

hang

e)

0

1

2

Fold

Cha

nge

-11

-6

-1

4

Log 2

(Fol

d C

hang

e)

CPEB1

Eif4a3 Fzr1

Rpl26

Rpl36

Rpl5

Rps17

actin

Cd151

GAPDH

Mapk3

Med7

Miox

paip2

Pfdn5

QarsRim

kla

Stx5a

Tinf2

-5

-1

Log 2

(Fo

ld C

hang

e)Polysome microarray

Polysome qPCR

RNP qPCR

Total qPCR

Decreased unstable Decreased stable Chen_Fig.S3

D

A

BGV MI MII

0

1000

2000

3000

4000

*

**

Fluo

resc

ence

Inte

nsity

(arb

itrar

y un

its)

0 2 4 60.0

2.5

5.0

7.5

10.0

*

**

14

**

Time after hCG (h)

CC

NB

1 pr

otei

n le

vels

(Fol

d C

hang

e)

E

Chen_Fig.S4

C

GV MI MII0.0

2.5

5.0

7.5PolysomeSubpolysome/RNP

0

25

50

75

100

125

Fold

Cha

nge

(pol

ysom

e qP

CR

) % of G

V stage(total and R

NPs qPC

R)

Total qPCR

Chen_Fig.S5

0

1000

2000

3000

4000

5000

6000

7000

8000 Snapc5Tomm6PisdNdufb5Gnl3Glrx3RNAset2bDnajb6Polr3hTrappc2lCdc2aSpc24Rps27

GV MI MIIFl

uore

scen

ce In

tens

ity(A

ribi

trar

y U

nits

)

0

1000

2000

3000

4000

5000 Pxmp4SnRpbChcd10Rps20Nrd1Map2k4Wdr91EndogAspscr1Ai413782SnrbpElovl1Glrx3

GV MI MII

Fluo

resc

ence

Inte

nsity

(Ari

bitr

ary

Uni

ts)

Anapc

1(143

4444

_s_a

t)

Anapc

1(143

4443

_at)

Anapc

1(141

5680

_at)

Anapc

2(141

5707

_at)

Anapc

4(142

3930

_at)

Anapc

4(142

3931

_s_a

t)

Anapc

5(143

5177

_a_a

t)

Anapc

5(143

5178

_x_a

t)

Anapc

5(141

6906

_at)

Anapc

5(143

3822

_x_a

t)

Anapc

5(145

3307

_a_a

t)

Anapc

5(145

6695

_x_a

t)

Anapc

7(141

8458

_at)

Anapc

10(14

2937

5_at)

Anapc

10(14

2937

6_s_

at)

Anapc

13(14

5258

6_at)

Cdc16

(1425

554_

a_at)

Cdc20

(1416

664_

at)

Cdc27

(1426

076_

at)

Cdc27

(1433

848_

at)

Cdc27

(1433

849_

at)

Cdc27

(1454

739_

at)

Cdc26

(1423

987_

at)

Fzr1(14

1945

1_at)

0

1 0 0 0

2 0 0 0

3 0 0 0

4 0 0 0

5 0 0 0

G VM IM II

Fluo

resc

ence

Inte

nsity

(Arb

itrar

y Un

its)

Spindle Assembly Checkpoint (SAC)

Mad2l1

(1422

460_

at)

Mad2l2

(1460

348_

at)

Bub3(1

4168

15_s

_at)

Bub3(1

4484

73_a

t)

Bub1b

(1416

961_

at)

Bub1b

(1447

362_

at)

Bub1b

(1447

363_

s_at)

0

1000

2000

3000

4000

5000

6000

7000

8000GVMIMII

Fluo

resc

ence

inte

nsity

(arb

itrar

y un

its)

Apc1

Apc2Apc11

Apc5

Apc4Cdc23Cdc16

Cdc27

Apc13Cdc26

Apc10

Apc9

APC core

=

APC/cyclosome components

Cohesin subunits

stag2

(1421

849_

at)sta

g2(14

5039

6_at)

stag1

(1431

921_

a_at)

stag1

(1421

940_

at)

stag1

(1431

921_

a_at)

stag1

(1434

189_

at)

0

250

500

750

1000

1250

GVMIMII

Fluo

resc

ence

Inte

nsity

(arb

itrar

y un

its)

Chen_Fig.S6

-40

-30

-20

-10 0 10 20 30 40 50 60 70 80 90 100

110

120

130

140

150

160

170

180

190

200

210

220

230

240

250

260

270

280

2900

10

20

30

40

50

60

70

80

801 unique regulated transcripts with one or more CPEs

Relative position of the putative CPE closest to the Hexamer

Num

ber

of tr

ansc

ript

s

Chen_Fig.S7

0 1 2 3 4 5 6 7 8 9 10 14-10.0

-7.5

-5.0

-2.5

0.0

2.5

5.0

7.5

Number of putative CPEs/transcript

Fold

cha

nge

(log2

)

1371 538 323 155 97 51 27 15 13 4 4 3

Chen_Fig.S8

-8.25

-7.75

-7.25

-6.75

-6.25

-5.75

-5.25

-4.75

-4.25

-3.75

-3.25

-2.75

-2.25

-1.75

-1.25

-0.75

-0.250.2

50.7

51.2

51.7

52.2

52.7

53.2

53.7

54.2

54.7

55.2

55.7

5

0.00

0.05

0.10

0.15

0.20None2 or more CPE

1/fr

eque

ncy

dist

ribu

tion

-8.25

-7.75

-7.25

-6.75

-6.25

-5.75

-5.25

-4.75

-4.25

-3.75

-3.25

-2.75

-2.25

-1.75

-1.25

-0.75

-0.250.2

50.7

51.2

51.7

52.2

52.7

53.2

53.7

54.2

54.7

55.2

55.7

5

0.00

0.05

0.10

0.15

0.20None2 or more Dazl

1/fr

eque

ncy

dist

ribu

tion

Class IClass IIClass III

-8.25

-7.75

-7.25

-6.75

-6.25

-5.75

-5.25

-4.75

-4.25

-3.75

-3.25

-2.75

-2.25

-1.75

-1.25

-0.75

-0.25 0.2

50.7

51.2

51.7

52.2

52.7

53.2

53.7

54.2

54.7

55.2

55.7

5

0.00

0.05

0.10

0.15

0.20 NoneBoth CPE and Dazl

Log2 Fold Changes

1/ r

elat

ive

dist

ribu

tion

Chen_Fig.S9A

B

C

Chen_Fig.S10

Cpeb1

Cpeb3

Cpeb4 Daz

l

Pum2

Msi2

Dazap

1

Dazap

2

Dzip3

0

1000

2000

3000

4000

5000MIIMIGV

*

***

*

****

**Fl

uore

scen

ce In

tens

ity(A

rbitr

ary

Uni

ts)

Chen_Fig.S11

Cpeb1

Cpeb3

Cpeb4

Pum2

Dazl

0

25

50

75 MOCon

Perc

ent P

olar

Bod

y

MO-Con

MO-Daz

l

MO-Daz

l + hD

AZL RNA

0

2 5

5 0

7 5

*

NS

Perc

ent P

olar

Bod

y

MO-Con

MO-Daz

l

MO-Daz

l + hD

AZL*

0

2 5

5 0

7 5

* *

Perc

ent P

olar

Bod

y

A

Chen_Fig.S12

B C

MO-DAZL+ hDAZL

MO-Con

DIC DAPI Tubulin DAZL Merged A

Chen_Fig.S13

B DAZL Pericentrin Merged

Eif4a3

Gapdh ZP3

DAZLTex

19.1

Bub1b

cdc2

0Tpx

2Arid

1asm

arca5

-2

-1

0

1

2

No Dazlelement

1 or more putativeDazl element

Log 2

Fol

d C

hang

e(r

elat

ive

to C

ontr

ol)

Chen_Fig.S14

2 cell 4-8 cell morula0

25

50

75

100MO-controlMO-DazlMO-Dazl + hDAZL

ND

Dev

elop

ing

Embr

yos

(%)

MO-DazlMO-control MO-Dazl + hDAZL

Chen_Fig.S15

DAZL

ATGTAA

5’3’

ATGTAA5’

3’

ATG TAA AAAA’

DAZL

DAZL

DAZL

Metaphase II

CPEB1

ATGTAA

3’

ATGTAA

3’

ATG TAA 3’

DAZL

ATG TAA 3’

DAZL

DAZL

ATGTAA

’3’

CPEB1

CPEB1

ATGTAA

3’

Phase-specific control of mRNA translation during mouse oocyte maturation

CPEB1Dazl mRNA

AAAAAAA

AAAAAAA

AAAAAAA

AA

Prometaphase I

CPEB4

?

ATGTAA

AAA

Dazl mRNA

Anaphase I

Positivefeedback

Chen_Fig.S16