JNCI REVISED MANUSCRIPT CHECKLIST

This checklist describes JNCI style requirements and should be followed carefully during manuscriptrevision. Please upload a completed checklist with your revised manuscript files when you complete yourrevised manuscript submission.

Letters, format, and supplementary files

Provide a Response to Reviewer letter with the reviewer comments and responses, explaining either the change made toaccommodate each comment or the reason for not making a change. Pay particular attention to starred comments, if anyare included.Provide permission letters for personal communications,from named individuals who supplied materials (e.g., cell linesor probes) or made substantive intellectual contributions (e.g., to statistical analysis), or who are acknowledged in theNotesProvide permission letters for all copyrighted material. Avoid the use of copyrighted material (e.g., original, minimallyaltered, or partial versions of tables and illustrations) if possible; otherwise, obtain written permission from the copyrightholder for use by the JNCI and Oxford University Press in print form, microfiche, and all electronic formats forworldwide use for the life of the work. Also obtain permission from the author of the original material.Provide a "red-lined" version of the manuscript, in which changes made in response to the reviewers' comments havebeen highlighted (e.g., by using red font, underlining, or yellow highlighting).Tables should be included at the end of the manuscript file rather than uploaded separately; upload figures as separatefiles (there should not be more than one figure in a figure file, but all panels of a figure should be in the same file).The number of supplementary materials per manuscript should not exceed the total number of figures and tables allowedfor the specific manuscript type submitted. If more materials are submitted, a statement to justify why it is necessary tosubmit more must be included with the submission.Any supplementary figures, tables, data sets, or methods should be uploaded as a single file. If a supplementary file istoo large to be combined with the other supplemental material, an additional supplemental file may be included.However, in the Description window, please write "Large File" followed by the description of the file (e.g. "Large File -Supplemental Table 3"). Supplemental material of any kind should not be included in the Manuscript file.

Length limits

Abstract is no more than 250 words for an Article, MiniReview, Review, or Commentary and no more than 150 words fora Brief Communication. Articles, Systematic Reviews, and Meta-analyses have a structured abstract; other manuscripttypes have an unstructured abstract.

Word count and number of figures/tables are correct. Limits are as follows:

Manuscript type Word limit* Figure + table limit†Article 3000 8Brief Communication‡ 1000 2MiniReview 2000 4Review§ 6000 8Commentary 2000-4000 4

*Not including Abstract, references, figure legends, tables, and footnotes.† The limit is the total number of figures and tables combined, not for each separately. TheCONSORT-style trial flow diagram required for reports of clinical trials does not count toward thefigure/table limit.‡The text of a Brief Communication is not divided into sections.§Systematic Reviews (with or without Meta-analysis) and Meta-analyses are also in the Reviewscategory but are formatted like Articles. The 6000-word limit includes the Methods section.

General requirements

Title is 14 words or less, does not state results, and is not a full sentence or a question.Subheadings are not full sentences and do not state results.All information in the Abstract is also present in corresponding sections (ie, Methods and Results) of the text.Quantitative data are included in the Abstract for the key findings.References and in-text citations are in JNCI style (see Instructions to Authors).Report quantitative data for the main/primary outcomes. If numerical data are in Tables, they do not need to be repeatedin the Results text, unless they are presented in the Abstract. If primary results are shown in Figures, the numbers(means, estimates, rates, etc., along with 95% confidence intervals, and P values, if applicable) must be reported in theResults text.For studies involving human subjects, the Methods section states that written informed consent was obtained (incountries in which such consent is required) and gives the name of the committee(s) that provided approval for the study.For studies involving animals, the Methods section states that animal care procedures followed recommendations fromappropriate committees.GenBank Accession numbers are provided in papers with sequences.HUGO nomenclature is used to identify gene symbols.Reference(s) to tumor grading and staging systems has been provided.A paragraph stating the study limitations is included in the Discussion section.The word "significant" is modified according to whether it denotes statistical, biological, or clinical significance. Theword "correlated" is used only when a formal statistical test for correlation, along with a correlation coefficient and Pvalue, is provided.The role of the study sponsor(s) in the design of the study; the collection, analysis, and interpretation of the data; thewriting of the manuscript; and the decision to submit the manuscript for publication is described in the Notes section.

Figures

Low-resolution figure files are supplied as per Instructions to Authors.All details (lettering, symbols, patterns, and lines) in each figure will be clear when reduced for publication (usually at 3to 4 inches wide). See "Figures and Tables" in Instructions to Authors regarding background shading, labeling, etc.Micrographs include scale bars, including in Supplementary Figures.

Tables

Tables must be in Word table format. Explanatory notes are indicated beneath (not in) the body of the table, using the

following symbols, in this order: *, †, ‡, §, ||, ¶, and #. These symbols should be repeated, doubled (**, ††, etc.)and then tripled (***, †††, etc.), if more symbols are needed.Empty cells should contain an em-dash and a footnote explaining why no value is given.

Data presentation

A separate Statistical Analysis subsection is included in the Methods section. Tests of statistical significance are two-sided, and a statement to this effect is included in the Abstract and text; if tests

must be one-sided, strong appropriate justification is given. The cut-off for statistical significance has been stated.Exact P values are provided (unless P<.001); the statistical test used is specified. (If P is between 0.99 and 0.01, report totwo decimal places. If between 0.009 and 0.001, report to three decimal places.)

For all outcome variables, 95% confidence intervals (rather than standard errors) or standard deviations are provided toshow precision of the estimates. HRs and ORs should be reported to two decimal places, and percentages should bereported to one decimal place throughout all manuscript materials.

When Cox proportional hazards analysis has been carried out, a statement is included noting how the assumption ofproportionality was verified.

Claims of synergism have been supported by formal calculations for synergy.

Clinical and Epidemiological Studies

Clinical trials are registered in a public database, and a registration number has been provided. Kaplan–Meier curves give—at several intervals—the number of patients or animals at risk. For randomized controlled trials, a CONSORT trial flow diagram and checklist have been provided; see

http://www.consort-statement.org/statement.html or The American Medical Association Manual of Style, 10th ed. Whenever possible, protocols and questionnaires have been provided as supplementary data for posting online. The reference category or group is identified for odds ratios and relative risks presented in tables. Causal language is not used to describe associations determined in epidemiological studies. Where appropriate, the data have been analyzed for differences by sex and by any of the major ethnic groups. Absolute risks of outcomes with and without the intervention or exposure have been calculated from the population rate

(even if the association between outcome and exposure has not been shown to be causal) and presented in the Results.When appropriate, the number of patients needed to treat to get one more (or less) case has been calculated.