filtration l tds sample vol. dw vol. dil factor (ml) kits eflochem i pool_low.pdf · 100 ml shaker...

EFLOCHEM CHEMICALS • P.O. BOX# 12282 • DUBAI-UAETEL:# +9714 3472900 • FAX# +9714 3477072 • [email protected] • www.eflochem.com

Sample Vol.(ml)

DW Vol.(ml)

Dil Factor

WATER SAMPLING POINTS

Feedwater: From main feedline (as close as possible the boiler).

Boiler: From Boiler Blowdown, as instructed by the manufacturer. Sample should be cooled to < 26° C. Sampling Procedure: Flush sampling line a few minutes then reduce the flow for 3-5 minutes with no disturbance. Flush the sample bottles a few times with the sample water. Collect the sample in the bottle w/o touching the sampling line. If the analysis is to be delayed, sample must be kept in tightly closed sample bottles.

< 28 Bar28 - 56 Bar

&

/ TDS

Oxygen Scavengers

While conducting water testing, there are cases in which collected samples may be turbid. This complicates the end point color & the results may not be correct. In such cases, filtration of the turbid sample with a special filter paper . Is necessaryAfter filtration it may be necessary to dilute the sample, if it is still turbid, by adding distilled water (DW). The result of the test is multiplied by the corresponding factor which is the dilution factor, as given below.

FILTRATION

Determination of Alkalinity MCaCO (10 - 1250 mg/l)3

K (0.2 - 25 mmol/l)S4.3

Attention!Wear protective gloves and safety gogles when performing any tests using corrosive, harmful or irritant reagents. Do not ingest.

CaCO3

mg/lKS4.3

mmol/l°dH*(KH)

°e*(CH)

°f*(DC)

1 mg/l CaCO3

1 0.02 0.056 0.07 0.1

1 mmol/lKS4.3

50 1 2.8 3.5 5.0

Conversion factors (Alka-M)

SVZ500-550Kit contains:

Black plastic carrying case100 ml shaker tube (SVZdev100)

Stirring rod (SPstr1)Cleaning brush (SPclb1)

250 tablets Total Alkalinity (M) (TbsRTA250)250 tablets Alkalinity P (TbsRAP250)


Alka-M

Alka-P

Alka-M / Alka-P Result

20 ml 50 ml 100 ml

1 50 20 10

2 150 60 30

3 250 100 50

4 350 140 70

5 450 180 90

6 550 220 110

7 650 260 130

8 750 300 150

9 850 340 170

10 950 380 190

11 1050 420 210

12 1150 460 230

13 1250 500 250

Low result! Increase product dose to achieve additional 100 mg/l Total Alkalinity/Alkalinity P

Correct level!

Reduce byincreased blowdown

N° of tablets

Results

* Retain the sample after theAlkalinity test as this maybeused for the Chloride determination if required!

1. Fill the 100ml shaker tube to the 20 ml mark: range 50 - 1250 mg/l CaCO 3

50 ml mark: range 20 - 500 mg/l CaCO 3


2. Add 1 tablet Total Alkalinity at a time with gentle mixing till the color changes from yellow to pink/reddish. Count the number of tablets used. *

1. Fill the 100ml shaker tube to the 20 ml mark: range 50 - 1250 mg/l CaCO 3


100 ml mark: range 10 - 250 mg/l CaCO3

2. Add 1 tablet Alkalinity P at a time with gentle mixing till the color changes from blue to yellow. Count the number of tablets used. *

Determination of Alkalinity PCaCO (10 - 1250 mg/l)3

-1. Use 2 ml to measure range 500 - 5000 mg/l Cl or 800 - 8000 mg/l NaCl- Use 10 ml to measure range 100 - 1000 mg/l Cl or 160 - 1600 mg/l NaCl

- Use 50 ml to measure range 20 - 200 mg/l Cl or 32 - 320 mg/l NaCl- Use 100 ml to measure range 10 - 100 mg/l Cl or 16 - 160 mg/l NaCl

2. Add one tablet of Chloride with gentle mixing. The sample should turn yellow.3. Add tablets of Chloride one at a time with gentle mixing until the brown colour disappears. Count the number of tablets used.

Determination of Chloride-Cl (10 - 5000 mg/l)

NaCl (16 - 8000 mg/l)

SVZ1600Kit contains:


Stirring rod (SPstr1)Cleaning brush (Spclb1)

250 tablets Chloride (TbsRCD250)



-Clmg/l

NaClmg/l

mg/l 1 1.6

Conversion factors

-Cl NaCl

2 ml 10 ml 50 ml 100 ml 2 ml 10 ml 50 ml 100 ml

1

2 500 100 20 10 800 160 32 16

3 1000 200 40 20 1600 320 64 32

4 1500 300 60 30 2400 480 96 48

5 2000 400 80 40 3200 640 128 64

6 2500 500 100 50 4000 800 160 80

7 3000 600 120 60 4800 960 192 96

8 3500 700 140 70 5600 1120 224 112

9 4000 800 160 80 6400 1280 256 128

10 4500 900 180 90 7200 1440 288 144

11 5000 1000 200 100 8000 1600 320 160

N° of tablets

Results

-20 - 70 mg/l Cl : Maintain daily blowdown!-100 - 220 mg/l Cl : Acceptable chlorides in low pressure boiler!-240 - 400 mg/l Cl : Reduce chlorides by increased blowdown!

Note!If measuring boiler water, it is possible to use the completed Alkalinity test sample, then follow instructions from step 2 above.

1. Decide about the range you want to test. As higher the range as less is the accuracy. Range 70 - 700 mg/l: Take 20 ml sample and fill up with 30 ml of deionised water. Range 140 - 1400 mg/l: Take 10 ml sample and fill up with 40 ml of deionised water. Range 280 - 2800 mg/l: Take 5 ml sample and fill up with 45 ml of deionised water. Range 560 - 5600 mg/l: Take 2,5 ml sample and fill up with 47,5 ml of deionised water.2. Add two tablets of Nitrite No. 1 with gentle mixing.3. Add one tablet Nitrite No. 2 with gentle mixing. 4. Wait for 1 minute.

If the solution remains colourless, repeat step 3 - 4. If the solution turns and remains pink, remember the amount of Nitrite No. 2 tablets used and read the result from the following table:

Determination of NitriteNaNo (70 - 5600 mg/l)2




100 tablets Nitrite No. 1 (TbsHNT1100)250 tablets Nitrite No. 2 (TbsRNT2250)Attention!

Wear protective gloves and safety gogles when performing any tests using corrosive, harmful or irritant reagents. Do not ingest.


50 ml 50 ml 50 ml 50 ml

2,5+47,5 5+45 10+40 20+30

1 560 280 140 70

2 1120 560 280 140

3 1680 840 420 210

4 2240 1120 560 280

5 2800 1400 700 350

6 3360 1680 840 420

7 3920 1960 980 490

8 4480 2240 1120 560

9 5040 2520 1260 630

10 5600 2800 1400 700

N° of Nitrit No.2

tablets

Results

200 - 1600 mg/l: The product is undertreated for all engine types and should be added to correct the Nitite level.1800 - 2200 mg/l: Sufficient product for normal speed engines using water that contains up to 50 ppm Chloride!2400 - 2800 mg/l: Sufficient product for normal/high speed engines using water that contains up to 50 ppm Chloride.3000 - 3400 mg/l: Product for high speed engines using water that contains up to 50 ppm Chloride!


1. Fill shaker tube to 20 ml mark: range 50 - 1250 mg/l CaCO3


100 ml mark: range 10 - 250 mg/l CaCO3

2. Add one tablet of Total Hardness with gentle mixing. The sample should turn light purple.3. Add tablets of Total Hardness one at a time with gentle mixing until the blue colour disappears. Count the number of tablets used.

Determination of Total HardnessCaCO (10 - 1250 mg/l)3




250 tablets Total Hardness (TbsRTH250)


CaCO3

20 ml 50 ml 100 ml

1 50 20 10

2 150 60 30

3 250 100 50

4 350 140 70

5 450 180 90

6 550 220 110

7 650 260 130

8 750 300 150

9 850 340 170

10 950 380 190

11 1050 420 210

12 1150 460 230

13 1250 500 250

N° of tablets

Results

K *S4.3

mmol/lmval/l* °dH

(KH)°e(CH)

°f(DC)

1 mg/l CaCO3

0.01 0.02 0.056 0.07 0.1

Conversion factors

Note!To obtain higher ranges,please dilute the sample!


1. Fill shaker tube to 20 ml mark: range 5 - 125 mg/l Na SO2 3

50 ml mark: range 2 - 50 mg/l 100 ml mark: range 1 - 25 mg/l2. Add one tablet of Sulphite No. 1 with gentle mixing. The sample should turn light pink.3. Add tablets of Sulphite No. 1 one at a time with gentle mixing until the pink colour disappears and the solution remains colourless. 4. Add one tablet of Sulphite No. 2 LR with gentle mixing. 5. Add tablets of Sulphite No. 2 LR one at a time with gentle mixing until the colour turns and remains blue. Count the number of tablets used.

Determination of Sulphite Low RangeNa SO (1 - 125 mg/l)2 3




250 tbl. Sulphite No. 1 (TbsHSPI1250)250 tbl. Sulphite No. 2 LR (TbsRSPIL2250)


Na SO2 3

20 ml 50 ml 100 ml

1 5 2 1

2 15 6 3

3 25 10 5

4 35 14 7

5 45 18 9

6 55 22 11

7 65 26 13

8 75 30 15

9 85 34 17

10 95 38 19

11 105 42 21

12 115 46 23

13 125 50 25

N° of Sulphite No. 2 LRtablets

Results


1. Fill shaker tube to 20 ml mark: range 50 - 1250 mg/l Na SO2 3

50 ml mark: range 20 - 500 mg/l 100 ml mark: range 10 - 250 mg/l2. Add one tablet of Sulphite No. 1 with gentle mixing. The sample should turn light pink.3. Add tablets of Sulphite No. 1 one at a time with gentle mixing until the pink colour disappears and the solution remains colourless. 4. Add one tablet of Sulphite No. 2 HR with gentle mixing. 5. Add tablets of Sulphite No. 2 HR one at a time with gentle mixing until the colour turns and remains blue. Count the number of tablets used.

Determination of Sulphite High RangeNa SO (10 - 1250 mg/l)2 3




250 tbl. Sulphite No. 1 (TbsHSPI1250)250 tbl. Sulphite No. 2 LR (TbsRSPIL2250)


Na SO2 3

20 ml 50 ml 100 ml

1 50 20 10

2 150 60 30

3 250 100 50

4 350 140 70

5 450 180 90

6 550 220 110

7 650 260 130

8 705 300 150

9 850 340 170

10 950 380 190

11 1050 420 210

12 1150 460 230

13 1250 500 250

N° of Sulphite No. 2 HRtablets

Results


Comparator Kit Phosphate PO (0 - 80 mg/l)4

Chlorine (0.0 - 1.0 / 4.0 ppm)

Kit contains:Black plastic carrying case

Comparator (PCP001)4 x 10 ml cell (SPkv5)


Disc for Phosphate (FD1200)Disc for Chlorine (FD101)

250 tbl. Phosphates Comp.(TbsRPP250)250 tbl. DPD No. 1 Photometer (TbsPD250)250 tbl. DPD No. 3 Photometer (TbsPD250)


5ml

10ml

15ml

5ml

10ml

15ml

C D

5ml

10ml

15ml

5ml

10ml

15ml

10 ml

5ml

10ml

15ml

1 2 3

5

C D

E

D

for Phosphate: Phosphate HR Comparator tabletfor Chlorine: DPD 1 Photometer tablet

1 x

4Disc: for Phosphate: FD1200for Chlorine: FD101

DC

x

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

BA

x

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

x

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

Only for Total Chlorine / Combined Chlorine:

1 5a)

b)

5ml

10ml

15ml

DPD 3 Photometer1 x

D

+ 3 5 = Total Chlorine!

5 b

0

Combined Chlorine

5 a

= DPD 1 + DPD 3

D

Note!If samples are turbid, filter before testing.

5ml

10ml

15ml

A Comparator B Disc C Cell - only sample D Cell - sample + tabletE Stirring rod



PERMANGANATE VALUE (PV)(BOD / COD & TOC)

The Permanganate Value is determining the general quality of Final Effluents in sewage. Based on the results it will give the OK for the discharge of Sewage water. The test procedure is relatively easy and consists of 4 steps:

Ÿ Three sample containers in the set must be filled with 100 ml of Sewage Effluent.Ÿ Add two Acidifying SE tablets per sample container and shake it to mix well.Ÿ Add one permanganate value tablet to the first sample container, then add two permanganate tablets to the second and three tablets to the third, and again shake them well until it all of them are mixed and dissolved.Ÿ Wait 30 minutes! Then check how many tubes have remained pink and read the result from the following table:

Container Pink Permanganate Value Grading

3

2

1

None

0 - 10

10 - 20

20 - 30

30 or more

Perfect

Satisfactory

Dubious

Not allowed to discharge

In the Test Kit you will also find instructions for the testing of Crude waste, the same is valid for Settled sewage.

BOD / COD & TOC

BOD = Biochemical Oxygen Demand COD = Chemical Oxygen Demand TOC = Total Organic Carbon

The results from this test are in connection with the Permanganate Value (PV) as done in paragraph1. to convert the PV for domestic sewage and effluent to probable BOC, COD and TOC values multiplyby the following factors:

Sewage Effluent

Probable BOD

Probable COD

Probable TOC

PV x 5

PV x 10

PV x 3

PV x 1,5

PV x 7

PV x 2

In general there is a connection between the Turbidity and the BOD-value of settled sewage and efluent. The BOD can also be calculated from the result of the turbidity test usig the fomula:

Probable BOD = + 5Turbidity

2

BOD-values can be cross checked that way with the PV tests. Recommendation for Effluent is not more than 20 mg/l.

Kit contains:3 x 100 ml shaker tube (SVZdev100)


200 tablets Acidifying SE (TbsHASE200)100 tablets Permanganate Value (TbsPPMGNV100)


Electronic Meter FT15pH 0.00 - 14.00(0.0 - 50.0 °C)

Ÿ 1 point manual calibration (pH)Ÿ Incl. 4 x 1.5 V batteriesŸ In a dark blue plastic caseŸ English manual

Operation:

Make sure the tester is calibrated to pH 7.001) Remove protective cap and switch meter on.2) Immerse electrode in solution to be tested.3) Stir gently and wait for the reading to stabilize.4) Press “°C/°F“ to switch between Celsius and Fahrenheit.5) Read the result, than rinse the electrode with water.6) Replace the protective cap and switch the meter off.7) Make sure the electrode is kept wet all the time!

Buffer solution:

20 ml sachet / 7.00 pH / 1-time-use


Operation:Calibration:

Battery:

FT15 “7.00 PH“

4


Electronic Meter FT33EC / Electronic conductivity: 0.00 - 19.99 mS/cm

Ÿ 1 point manual calibrationŸ Incl. 4 x 1.5 V batteriesŸ In a leather like bag with belt clip

Operation:Calibration:

Battery:


Operation:

Make sure the tester is calibrated to 1413 µS/cm1) Remove protective cap and switch meter on.2) Immerse electrode in solution to be tested.3) Stir gently and wait for the reading to stabilize.4) Read the result, than rinse the electrode with water.5) Replace the protective cap and switch the meter off.

Buffer solution:

20 ml sachet / 1413 µS/cm / 1-time-use

4

Organism

Colony Size (mm)

Shape & Surface

Colour

Comments

Pseudomonas Aeruginosa

Spreading

Glossy, Thin

Yellow-Green

PDM

P. fragi 1.0 - 3.0 CVEG Grey PDM

E. aecalis 1.0-2.0 CVEG Red MacConkey No. 3

E. coli 1.5-2.5 CVEG Pink/ Red MacConkey No.3

S. typhimurium

0.5-1.0

FED

Colourless

McConkey No. 3

CV.E.G = Convex entirely glossy, FED = Full entire dull



Bacteria Testing BactD006Pseudomonas species and Coliforms

Ÿ 10 PDM / MAC Dipslide TestsŸ For potable water, pools and spasŸ Flexible paddle for effective surface contact

The PDM/MAC dipslide allows convenient enumeration of Pseudomonas spp. on the pale strawPseudomonas differential medie (PDM) and enterobacteriaceae on the reddish/brown MacConkeyside. PDM agar is a differential media that selects for Pseudomonas spp, while MacConkey agar isdifferential, all in one test for gram negative bile tolerant enterobacteriaceae.

SAMLLING: AIRThe sample should be taken by inverting the paddle and inserting in the vial. Let it sit for 15 minutesexpodes to the air, the replace the paddle in the vial and sela tightly.

SAMPLING: FLUIDSThe sample should be taken by immersing both sides of the paddle into the fluid to be tested, it havingfirst been removed from the sterile container. Ecxess sample sould be gently shaken from the paddlebefore it is replaced in the container.

SAMLPLING: SURFACESThe sample should be taken by allowing direct contact between te agar surface and the test material.The paddle is flexible and can be bent at the upper end to allow both surfaces to come into intimatecontact. Bacterial recovery rate is about 50% so that sweeping an area approximately twice that of thepaddle will give a more accurate result.

INCUBATION:Incubate at 30° C for 24-48 hours, when full ernumeration should be completed.

DISPOSALUsed slides should be incinerated or autoclaved. Alternatively, immerse in a 10% bleach solution for 24 hours.

Surface

AEROBIC MICROBES -

TVC Fluids

0/ / / / / 0/ / / / /

MACCONKEY AGAR:Colourless: Salmonella typhimurium /Proteus mirabilis / Pseudomonas aeruginosaPink: Aerobacter aerogenes Pink/Red: Escherichia coli Yellow/Green + Buff colonies: Total coliforms

PDM AGAR:Green/Blue: Pseudomonas aeruginosaBuff/Grey colonies: Other Pseudomonas Species Green/Blue and Buff/Grey: Total Pseudomonas

Approx Colony Count per 100 ml



Incubator BactEDT057-30

Organism

Colony Size (mm)

Shape & Surface

Colour

Comments

Pseudomonas Aeruginosa

Spreading

Glossy, Thin

Yellow-Green

PDM

P. fragi 1.0 - 3.0 CVEG Grey PDM

E. aecalis 1.0-2.0 CVEG Red MacConkey No. 3

E. coli 1.5-2.5 CVEG Pink/ Red MacConkey No.3

S. typhimurium

0.5-1.0

FED

Colourless

McConkey No. 3

CV.E.G = Convex entirely glossy, FED = Full entire dull

Surface

AEROBIC MICROBES -

TVC Fluids

0/ / / / / 0/ / / / /

MACCONKEY AGAR:Colourless: Salmonella typhimurium /Proteus mirabilis / Pseudomonas aeruginosaPink: Aerobacter aerogenes Pink/Red: Escherichia coli Yellow/Green + Buff colonies: Total coliforms

PDM AGAR:Green/Blue: Pseudomonas aeruginosaBuff/Grey colonies: Other Pseudomonas Species Green/Blue and Buff/Grey: Total Pseudomonas

Approx Colony Count per 100 ml

Ÿ Incubator for 2 dipslidesŸ Preset to 30° C

The PDM/MAC dipslide allows convenient enumeration of Pseudomonas spp. on the pale strawPseudomonas differential medie (PDM) and enterobacteriaceae on the reddish/brown MacConkeyside. PDM agar is a differential media that selects for Pseudomonas spp, while MacConkey agar isdifferential, all in one test for gram negative bile tolerant enterobacteriaceae.

SAMLLING: AIRThe sample should be taken by inverting the paddle and inserting in the vial. Let it sit for 15 minutesexpodes to the air, the replace the paddle in the vial and sela tightly.

SAMPLING: FLUIDSThe sample should be taken by immersing both sides of the paddle into the fluid to be tested, it havingfirst been removed from the sterile container. Ecxess sample sould be gently shaken from the paddlebefore it is replaced in the container.

SAMLPLING: SURFACESThe sample should be taken by allowing direct contact between te agar surface and the test material.The paddle is flexible and can be bent at the upper end to allow both surfaces to come into intimatecontact. Bacterial recovery rate is about 50% so that sweeping an area approximately twice that of thepaddle will give a more accurate result.

INCUBATION:Incubate at 30° C for 24-48 hours, when full ernumeration should be completed.

DISPOSALUsed slides should be incinerated or autoclaved. Alternatively, immerse in a 10% bleach solution for 24 hours.

filtration l tds sample vol. dw vol. dil factor (ml) kits eflochem i pool_low.pdf · 100 ml shaker...

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