first cloning experiment cohen et al., 1973 “recombinant dna”
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First Cloning Experiment
Cohen et al., 1973
“Recombinant DNA”
Cloning Vectors: pBR322 plasmid
Cloning into pBR322
RT-PCR and Cloning
Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615
“Supermice”
Gene Therapy: Adenovirus vector
Golden Rice
Weeding Out Land MinesFirst Glowing Pet
Polypeptide Polypeptide “X”“X”
Anti-”X” Anti-”X” AntibodiesAntibodies
•Antibodies can be Antibodies can be utilized to detect utilized to detect the presence of the presence of protein “X” in a protein “X” in a
given samplegiven sample
Antibody ProductionAntibody Production
Y YY
Y
Y Y
Western Blot AnalysisWestern Blot Analysis
SDS-PAGESDS-PAGE
Isolate ProteinsIsolate Proteins
P3P3
S6S6
Incubate Incubate with with
AntiseraAntisera
Transfer to nitrocellulos
e
YYYY
Proteomics Approach in Arabidopsis