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Fluorescence Intensity (GeneBLAzer)SpectraMax Detection Cartridge
Fluorescence Intensity SpectraMax® Detection Cartridges for SpectraMax® i3 and Paradigm® Systems provide speed, sensitivity and flexibility. The cartridge design provides uncompromising performance for both top and bottom reads. In addition, the standard dual-emission design enables simple, straightforward FRET (fluorescence resonance energy transfer) measurement.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. ©2013 Molecular Devices, LLC • 8/13 • Printed in USA • PN: 0120-1518.B
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Designed to provide optimal performance when used with Life Technologies’ GeneBLAzer reagents.
DescriptionCompatible systems ..............................................SpectraMax i3 and Paradigm ReadersPart Numbers .................................................................................................................... 0200-7006Mounting .............................................................................................. Top Read or Bottom ReadCartridge Size .......................................................................................................................1 PositionLight Source .............................................................................................................High-Power LEDWavelength-Tuned Excitation .....................................................................................406/15 nmWavelength-Tuned Emission ............................................................................ EM1 465/35 nm....................................................................................................................................... EM2 535/25 nm
Key applicationsCell-based assays
• GPCR Assays
• Ion Channel
• Nuclear and Cytokine Receptor Assays
• Signal Transduction Pathways
• Transcriptional Regulators (Kinases)
CO2
CO2
Cl
HNHN
SS
O
O O O
OO
N
O
O O
CO2
SH
O O O
CO2
Cl
HNHN S
O O O
O
447 nm409 nm
O
NO2C
520 nm
+beta-lactamase
Beta-lactamase
BLA Substrate
409 nm FRET -beta-lactamasegreen cells
blue cells
Principles of technology
Fluorescence Intensity (GeneBLAzer) SpectraMax Detection Cartridge
Fully enabledprotocols reduce
setup time