fluorescent product guide

Pierce ®

now sold as

Thermo Scientific

Featuring

• Thermo ScientificDyLight™ Dyesand Conjugates, Dye Removal Columns,Antibody Labeling Kits,Western Blotting Kits,and MW Markers

Thermo Scientific PierceFluorescent Products Guide

Table of Contents

DyLight Dye Summary.............................................................................................. 3

DyLight Amine- and Sulfhydryl-Reactive Dyes ........................................................ 4

NHS Ester and Maleimide

DyLight Dye Removal Columns ................................................................................ 7

Antibody Labeling Kits............................................................................................ 8

Microscale and Regular Antibody Labeling Kits

DyLight Conjugates................................................................................................. 10

DyLight Fluorescent Protein Molecular Weight Markers .......................................... 12

Infrared and Fluorescent Protein Molecular Weight Markers

DyLight Western Blotting Kits ................................................................................. 14

Krypton Fluorescent Protein Stain........................................................................... 16

Thermo ScientificFluorescent Products

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Bright New Alternatives to Alexa®, CyDye® and LI-CORFluorescent DyesThermo Scientific DyLight Dyes have absorption spectra ranging from 400 nm to 770 nm (Table 1)and match the principal output wavelengths of common fluorescence instrumentation. TheDyLight Dyes exhibit higher fluorescence intensity and photostability than Alexa, CyDye andLI-COR Dyes in many applications and remain highly fluorescent over a broad pH range (pH 4-9).Additionally, the water solubility of the DyLight Dyes allows a high dye-to-protein ratio withoutprecipitation during conjugation.

Table 1. Spectral properties of Thermo Scientific DyLight Fluorescent Dyes.

Emission DyLight Dye Ex/Em* ε† Spectrally Similar DyesBlue 405 400/420 30,000 Alexa 405 and Cascade BlueGreen 488 493/518 70,000 Alexa 488, fluorescein and FITCYellow 549 550/568 150,000 Alexa 546, Alexa 555, Cy3 and TRITCRed 633 638/658 170,000 Alexa 633Red 649 646/674 250,000 Alexa 647 and Cy5Near 680 682/715 140,000 Alexa 680 and Cy5.5Infrared 800 770/794 270,000 IRDye 800*Excitation and emission maxima in nanometers†Molar extinction coefficient (M-1 cm-1)

Thermo ScientificDyLight Dye Summary

Figure 1. Thermo Scientific DyLight 488 and DyLight 633 Dyes exhibit outstanding fluorescence in structuredillumination. The uniform fluorescence intensity throughout the images demonstrates the outstanding brightnessand photostability of DyLight 488 and 633 Dyes. Red: Alpha tubulin detected in HeLa cells with anti-tubulin mono-clonal antibody and DyLight 633 Dye-conjugated secondary antibody (highly cross-adsorbed). Green: Histone H4detected with anti-histone monoclonal antibody and DyLight 488 Dye-conjugated secondary antibody (highlycross-adsorbed). Blue: Nucleus counter-stained with fluorescent mounting media containing DAPI. Images wereacquired with the Axio Imager.Z1 and ApoTome Slider (Zeiss MicroImaging, Inc). The ApoTome Module providesconfocal-like resolution allowing optical sectioning without using a pinhole (e.g., confocal). No image enhance-ment was performed.

Thermo Scientific DyLight Amine-Reactiveand Sulfhydryl-Reactive DyesExcellent photostability make these dyes the clear alternative

Highlights:• Available in both amine- and sulfhydryl-reactive chemistries for fast and efficient labeling

of IgG or other proteins• High water solubility• Excellent photostability• Compatible with common fluorescence instrumentation

4

Dyes

Applications:• Fluorescence microscopy• Western blot detection • Protein arrays• Flow cytometry • ELISA • FRET-based technology• And many more

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Figure 1. Excitation and emission spectra for Thermo Scientific DyLight Dyes.

Exci

tatio

n

Emis

sion

DyLight 549

450 500 550 650600 700Wavelength (nm)

Abs max: 550 nmEm max: 568 nm

Exci

tatio

n

Emis

sion

DyLight 680

550 600 650 750700 800Wavelength (nm)


Exci

tatio

n

Emis

sion

DyLight 649

550 600 650 750700 800

Wavelength (nm)


Exci

tatio

n

Emis

sion

DyLight 800

650 700 750 850800 900Wavelength (nm)



Exci

tatio

n

Emis

sion

DyLight 405

300 350 400 500450 550

Wavelength (nm)


Exci

tatio

n

Emis

sion

DyLight 488

420370 470 520 620570 670

Wavelength (nm)


Exci

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n

Emis

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DyLight 633

550500 600 650 750700

Wavelength (nm)

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Dyes (Cont.)

Ordering InformationU.S.

Product # Description Pkg. Size PriceAmine-Reactive Dyes46400 DyLight 405 NHS Ester 1 mg $19946401 DyLight 405 NHS Ester 5 x 50 µg $15946402 DyLight 488 NHS Ester 1 mg $19946403 DyLight 488 NHS Ester 5 x 50 µg $15946407 DyLight 549 NHS Ester 1 mg $19946408 DyLight 549 NHS Ester 5 x 50 µg $15946414 DyLight 633 NHS Ester 1 mg $19946417 DyLight 633 NHS Ester 5 x 50 µg $15946415 DyLight 649 NHS Ester 1 mg $19946416 DyLight 649 NHS Ester 5 x 50 µg $15946418 DyLight 680 NHS Ester 1 mg $19946419 DyLight 680 NHS Ester 5 x 50 µg $15946421 DyLight 800 NHS Ester 1 mg $19946422 DyLight 800 NHS Ester 5 x 50 µg $159Sulfhydryl-Reactive Dyes46660 DyLight 405 Maleimide 1 mg $19946602 DyLight 488 Maleimide 1 mg $19946607 DyLight 549 Maleimide 1 mg $19946613 DyLight 633 Maleimide 1 mg $19946615 DyLight 649 Maleimide 1 mg $19946618 DyLight 680 Maleimide 1 mg $19946621 DyLight 800 Maleimide 1 mg $199

High-performance dye removal columnsConvenient new options for fluorescent antibody labeling

The same high performance dye removal spin-columns used in the Thermo Scientific DyLightAntibody Labeling Kits are now available separately.

Highlights: • Fast – remove free dye from conjugates in approximately 30 seconds• Efficient – purification resin provides outstanding conjugate recovery (Figure 1)• Minimal sample dilution

Dye Removal Columns

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Conjugate FITC Fluorescein RhodamineGoat anti-mouse IgG 79% 86% 94%Streptavidin 90% 81% 74%Average Recovery 85% 84% 84%

Figure 1. Thermo Scientific DyLight Dye Removal Columns provide outstanding conjugate recovery.


Product # Description Pkg. Size Price22858 Dye Removal Columns Kit $ 99

Includes: Purification Resin 5 mlSpin Columns 10 ea. Microcentrifuge Collection Tubes 20 ea.

100%

80%

60%

40%

20%

0%

85%

(n = 12) 84%

(n = 12) 84%

(n = 12) Conj

ugat

e Re

cove

ry

Label and purify antibodies in one hour Antibody labeling kits for fast and efficient labeling and exceptional recovery

The Thermo Scientific DyLight Antibody Labeling Kits were specifically developed for fast, efficient labeling of antibodies. Two convenient kit formats are available to accommodate variedlabeling requirements. The Antibody Labeling Kits contain all necessary components to performthree separate labeling reactions using 1 mg of IgG or similar quantities of other proteins. TheDyLight Microscale Antibody Labeling Kits contain all the necessary components to perform fiveseparate labeling reactions using 100 µg of IgG.

The labeling kits use high-performance spin desalting columns to provide exceptional dyeremoval and antibody recovery (Figure 1).

Highlights: • Fast – fluorescently label and purify protein

in approximately one hour• Amine-reactive dyes – label virtually any

protein• Pre-measured fluorescent dye – eliminate

the time, waste and hassle associated withweighing dye

• Efficient non-reacted dye removal• Minimal sample dilution• Spin column format eliminates the need for

column preparation, fraction screening andwaiting for protein to emerge from column

• Easy protocol (Figure 2)

Antibody Labeling Kits

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Figure 1. Thermo Scientific DyLight AntibodyLabeling Kits provide outstanding recovery.The percent recovery for DyLight AntibodyLabeling Kits is the average for 16 labelingreactions using three different antibodies. The percent recovery for DyLight MicroscaleAntibody Labeling Kits is the average for13 labeling reactions using three differentantibodies.

Table 1. Spectral properties of Thermo Scientific DyLight Fluorescent Dyes.

Emission DyLight Dye Ex/Em* ε† Spectrally Similar DyesBlue 405 400/420 30,000 Alexa 405 and Cascade BlueGreen 488 493/518 70,000 Alexa 488, fluorescein and FITCYellow 549 550/568 150,000 Alexa 546, Alexa 555, Cy3 and TRITCRed 633 638/658 170,000 Alexa 633Red 649 646/674 250,000 Alexa 647 and Cy5Near 680 682/715 140,000 Alexa 680 and Cy5.5Infrared 800 770/794 270,000 IRDye 800*Excitation and emission maxima in nanometers†Molar extinction coefficient (M-1 cm-1)

0

20

40

60

80

100

Thermo ScientificDyLight Antibody

Labeling Kit

81% 75%

Thermo ScientificDyLight Microscale

Antibody Labeling Kit

Ant

ibod

y Re

cove

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Step 1. Labeling reaction

Add antibody to vialcontaining pre-measured

dye. Incubate 1 hour at room temperature.

Apply labelingreaction to Spin

Desalting Column.

Recoverlabeled antibody.

Centrifuge

30 seconds

Step 2. Removal of excess fluorescent dye

Figure 2. Protocol summary for Thermo Scientific DyLight Antibody Labeling Kits.


Product # Description Pkg. Size Price53021 DyLight 405 Microscale Antibody Labeling Kit Kit $275

DyLight 405 NHS Ester 5 vials53025 DyLight 488 Microscale Antibody Labeling Kit Kit $275






DyLight 800 NHS Ester 5 vials

• Reaction Buffer, 1 ml• Spin Columns, 5 each

• Microcentrifuge Collection Tubes, 10 each• Purification Resin, 5 ml

Microscale KitsContain sufficient reagents to label and purify 5 x 100 µg of IgG In addition to contents listed below, all Microscale Kits include:

• Reaction Buffer, 1 ml• Spin Columns, 6 each

• Microcentrifuge Collection Tubes, 12 each• Purification Resin, 5 ml

Antibody Labeling KitsContain sufficient reagents to label and purify 3 x 1 mg of IgG or similar quantities of other proteinsIn addition to contents listed below, all Antibody Labeling Kits include:


Product # Description Pkg. Size Price53020 DyLight 405 Antibody Labeling Kit Kit $275

DyLight 405 NHS Ester 3 vials53024 DyLight 488 Antibody Labeling Kit Kit $275






DyLight 800 NHS Ester 3 vials

Thermo Scientific DyLight ConjugatesExcellent brightness make these conjugates a clear alternative

Highlights:• Available conjugated to commonly used secondary antibodies, streptavidin and NeutrAvidin

Biotin-Binding Protein• Molar ratio (dye:protein) optimized to provide excellent fluorescent intensity• Stable for 1 year at 4°C• Antibody conjugates are affinity-purified to minimize cross-reactivity

Conjugates

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Figure 1. Two-color infrared Western blot detection of p53 and cyclophilin Bknockdown using Thermo Scientific DyLight 680- and Thermo Scientific DyLight800-labeled secondary antibodies. Protein lysate from transfected A549 cells was separated using SDS-PAGE and transferred to PVDF membrane. Lane 1: MW marker,Lane 2: mock transfected sample, Lane 3: negative control siRNA, Lane 4: siRNAtargeted against p53 and Lane 5: siRNA targeted against cyclophilin. The mem-branes were imaged with the Odyssey Infrared Imaging System using the 700and 800 channels.

1 2 3 4 5

Western Blotting

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Figure 3. Thermo Scientific DyLight 549 Goat Anti-Mouse Conjugates are brighter than Alexa 555 Dyeconjugates in microplate-based assays. Microplateswere coated with Mouse IgG at the indicated concen-trations. DyLight 549 and Alexa 555 Conjugates werediluted 1:250 (from 1 mg/ml stock solutions) in PBS and100 µl was applied to each plate. Fluorescent intensitywas measured with a Tecan Safire Microplate Readerusing Cy3 Dye settings.

Microplate-Based Assays

Rela

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nten

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0

10,000

20,000

30,000

40,000

50,000

60,000

0 50 100 200150 250Biotinylated BSA (ng/well)

DyLight 649 StreptavidinDyLight 649 NeutrAvidinAlexa 647 Streptavidin

Rela

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Fluo

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nten

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0

5,000

10,000

15,000

20,000

25,000

30,000

35,000

40,000

45,000

50,000

0 200 400 800600 1,2001,000Mouse IgG (ng/well)

DyLight 549 Goat anti-mouse IgG

Alexa 555 Goat anti-mouse IgG

DyLight 800 StreptavidinIRDye 800 Streptavidin

DyLight 680 StreptavidinAlexa 680 Streptavidin

Rela

tive

Fluo

resc

ent I

nten

sity

4,000,000

8,000,000

12,000,000

16,000,000

0 2 4 86 1210Biotinylated BSA (ng per well)

Figure 2. Thermo Scientific DyLight 649 Streptavidinand Thermo Scientific NeutrAvidin Conjugates arebrighter than Alexa 647 Streptavidin Conjugates inmicroplate-based assays. Microplates were coatedwith biotinylated BSA at the indicated concentrations.DyLight 649 and Alexa 647 Conjugates were diluted1:250 (from 1 mg/ml stock solutions) in PBS and 100 µlwas applied to each plate. Fluorescent intensity wasmeasured with a Tecan Safire™ Microplate Readerusing Cy5 Dye settings.

Ordering InformationConjugates: Package size for these items is 1 mg at 1 mg/ml.

Product # / U.S. PriceDyLight DyLight DyLight DyLight DyLight

Description 488 Dye 549 Dye 649 Dye 680 Dye 800 DyeGoat Anti-Mouse 35502/$128 35507/$128 35515/$128 35518/$128 35521/$128IgG (H+L)Goat Anti-Rabbit 35552/$128 35557/$128 35565/$128 35568/$128 35571/$128IgG (H+L)Streptavidin 21832/$155 21837/$155 21845/$155 21848/$155 21851/$155NeutrAvidin 22832/$170 22837/$170 22845/$170 22848/$170 22853/$170Biotin-Binding Protein

Figure 4. Thermo Scientific DyLight 680 and ThermoScientific DyLight 800 Streptavidin Conjugates arebrighter than Alexa 680 or IRDye 800 Conjugates inmicroplate-based assays. Microplates were coatedwith biotinylated BSA at the indicated concentrations.Conjugates were diluted to 10 µg/ml in PBS and 100 µlwas applied to each plate. Fluorescent intensity wasmeasured with the Odyssey Infrared Imaging Systemusing the 700 and 800 channels.

Table 1. Spectral characteristics of Thermo Scientific DyLight Fluorescent Protein Molecular Weight Markers.

ExtinctionExcitation (nm) Emission (nm) Coefficient (min)

DyLight 547 Fluors 557 574 150,000 M-1 cm-1

DyLight 647 Fluors 652 673 250,000 M-1 cm-1

Fluorescent MW markers for Western blottingand in-gel detectionOne- or two-color fluorescence detection with one protein molecularweight marker

Fluorescent Thermo Scientific DyLight Infrared Protein Molecular Weight Markers have been optimized for direct visualization of marker proteins after SDS-polyacrylamide gel electrophoresis(PAGE) (Figure 1). Two convenient formats are available to accommodate fluorescence imagingrequirements in the visible or near-infrared region of the spectrum. Each protein in the mixture islabeled with two fluorescent dyes to provide flexible one- or two-color detection with common fluo-rescence imaging systems (Table 1). The markers are compatible with Western blotting (Figure 2)and can be detected by virtually any in-gel staining method (Figure 3). The DyLight ProteinMolecular Weight Markers consist of nine proteins with molecular weights in the range of 6Kto 200K.

Highlights: • Easily multiplexed – two excitation and

emission maxima enable one- or two-colorfluorescence detection

• Saves time – eliminate the need for awkwardmarking or overlay procedures

• Fluorescence and colorimetric – two detec-tion options available: in-gel or on-membrane

• Instrument-compatible – spectra arecompatible with common fluorescenceimaging systems

• Photostable – capture multiple imageswith no decrease in fluorescent intensity

Fluorescent ProteinMolecular Weight Markers

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Product # Description Pkg. Size Price22859 DyLight Infrared Protein Molecular Weight Markers 250 µl $199

Sufficient material for loading 50 gel lanes 26665 DyLight Fluorescent Protein Molecular Weight Markers 250 µl $199

Sufficient material for loading 50 gel lanes.† See patent information on inside back cover

Fluorescent and Colorimetric Detection of Thermo Scientific DyLight Fluorescent Protein Molecular Weight Markers

Figure 1. Panel A. Direct in-gel fluorescent detection. Marker proteins (5 µl) were separated in 4-20% Tris-glycinegels. The gels were imaged with the Kodak Image Station 2000MM using a five-minute exposure at f2.8 with the A.535/600 nm excitation/emission filter set and B. 625/700 nm excitation/emission filter set.

Figure 2. Fluorescent Western blot detection. Marker proteins (5 µl) were separated in 4-20% Tris-glycine gels andtransferred to A. nitrocellulose or B. PVDF membrane. Blots were imaged with the Typhoon 9410 at 500V PMT usingthe A. Cy3 Fluor and B. Cy5 Fluor laser settings.

Note: Proteins in the marker mix produce uniform fluorescent intensities in SDS-PAGE applications; however, variationsin protein-transfer efficiency affect fluorescent intensity. For example, high molecular weight proteins, such as myosin(200K), typically transfer less efficiently than low molecular weight proteins.

Figure 3. Colorimetric in-gel detection. Marker proteins (10 µl) were separated in 4-20% Tris-glycine gels and stainedwith A. Thermo Scientific Imperial Protein Stain† and B. silver stain.

Myosin (200K)

BSA (66K)

Protein A (42K)

Protein L (36K)

Peanut Agglutinin (28K)

Aprotinin (6K)

Figure 1A B

Figure 2A B

Figure 3A B

Phosphoylase B (97K)

Trypsin Inhibitor (20K)

Lysozyme (14K)

Multiplex Fluorescent Western Blotting KitsFluorescent Western blotting made easy

The fluorescence detection of two different targets on a single Western blot is now easier toperform with the new Thermo Scientific DyLight 680/800 and 549/649 Western Blotting Kits. Thekit provides a highly optimized and convenient format to save you the time and frustration ofhaving to evaluate reagents for compatibility with fluorescent Western blotting (Figure 1). Eachkit contains sufficient reagents for 10 Western blots and includes the new Thermo ScientificPierce Dual-Labeled Protein Molecular Weight Markers† and secondary antibodies conjugatedto DyLight 680 and 800 Fluorescent Dyes (Table 1). The DyLight 680/800 Western Blotting Kit usesDyLight 680 and DyLight 800 labeled secondary antibodies for imaging in the near-infrared region(e.g. LI-COR Odyssey Imaging System) of the spectrum (Figure 2).

Highlights: • Easily multiplexed – detect two different

targets on a single Western blot• Optimized format – protocol requires little

to no optimization, saving time and the frustration of having to evaluate and optimizereagents (e.g., secondary antibody dilutions,etc.)

• Plug-and-play – kits include DyLight-labeledsecondary antibodies, blocking buffer, washbuffer, dual-labeled fluorescent molecularweight markers and low-fluorescencemembranes

• Instrument-compatible – DyLight Fluor spectra match the principal output wavelengths of common fluorescenceinstrumentation

• Optimized format – provides low backgroundand high signal intensity

• Convenient – saves time and money associated with optimizing fluorescentWestern blots

Western Blotting Kits

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Table 1. Spectral properties of Thermo Scientific DyLight Western Blotting Kits.

Ex/Em*

550/568646/674

682/715770/794

ε†

150,000250,000

140,000270,000

Spectrally Similar Dyes

Alexa 546, Alexa 555, Cy3 and TRITC Alexa 647 and Cy5

Alexa 680 and Cy5.5IRDye 800

Figure 1. The Thermo Scientific DyLight 549/649Western Blotting Kit provides lower background andhigher signal in two-color Western blot detectioncompared to a competing fluorescent Western blottingkit. Proteins were separated in 4-20% Precise ProteinGels and transferred to low-fluorescence PVDF mem-brane. The membranes were blocked overnight in 1%BSA and target proteins were detected following manu-facturer-recommended protocols. Membranes wereimaged with the Typhoon 9410.

25 n

g

12 n

g6

ng3

ng1

ng

0.5

ng

1 ng

3 ng

6 ng

12 n

g

25 n

g

25 n

g

12 n

g6

ng3

ng1

ng

0.5

ng

1 ng

3 ng

6 ng

12 n

g

25 n

gM

W M

arke

rM

W M

arke

r

DyLight 549/649 Western Blotting Kit

ECL Plex Western Blotting Kit

Tubulin

TNFα

TNFα

Tubulin

Western Blotting KitDyLight 549/649 Western Blotting KitDyLight 549 Goat Anti-Mouse IgG (H+L)DyLight 649 Goat Anti-Rabbit IgG (H+L)DyLight 680/800 Western Blotting KitDyLight 680 Goat Anti-Mouse IgG (H+L)DyLight 800 Goat Anti-Rabbit IgG (H+L)*Excitation and emission maxima in nanometers†Molar extinction coefficient (M-1 cm-1)

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Product # Description Pkg. Size Price22855 DyLight 680/800 Western Blotting Kit Kit $199

Sufficient reagents for 10 Western blots.Includes: DyLight 680 Goat Anti-Mouse IgG (H+L) 120 µl

DyLight 800 Goat Anti-Rabbit IgG (H+L) 120 µl DyLight Infrared Protein Molecular Weight Markers 30 µlWash Buffer (30X) 200 mlSEA BLOCK Blocking Buffer 500 mlLow-Fluorescence PVDF Transfer Membrane 10 ea.

22854 DyLight 549/649 Western Blotting Kit Kit $199Sufficient reagents for 10 Western blots.Includes: DyLight 549 Goat Anti-Mouse IgG (H+L) 50 µl

DyLight 649 Goat Anti-Rabbit IgG (H+L) 50 µl Fluorescent Dual-labeled Protein MW Markers 60 µlWash Buffer (30X) 200 mlBlocker BSA in PBS (10X) 50 mlLow-Fluorescence PVDF Transfer Membrane 10 ea.

Complementary Products U.S.Product # Description Pkg. Size Price22859 DyLight Infrared Protein Molecular Weight Markers 250 µl $199

Sufficient material for loading 50 gel lanes.22860 Low-Fluorescence PVDF Transfer Membrane, 10/pkg. $ 78

0.2 µm 7 cm x 8.4 cm26665 DyLight Dual Fluor-Labeled Protein MW Markers 250 µl $19937527 SEA BLOCK Blocking Buffer 500 ml $12953056 DyLight 680 Antibody Labeling Kit Kit $275

Sufficient reagents to label and purify 3 x 1 mg of IgG53057 DyLight 680 Microscale Antibody Labeling Kit $275

Sufficient reagents to label and purify 5 x 100 µg of IgG.53062 DyLight 800 Antibody Labeling Kit Kit $275

Sufficient reagents to label and purify 3 x 1 mg of IgG.53063 DyLight 800 Microscale Antibody Labeling Kit Kit $275

Sufficient reagents to label and purify 5 x 100 µg of IgG.† See patent information on inside back cover

Tubulin

1 µg

0.5

µg

0.25

µg

0.12

5 µg

0.06

3 µg

6.25

µg

12.5

µg

25 µ

g

50 µ

gTNFα

Figure 2. The Thermo Scientific DyLight 680/800Western Blotting Kit provides low background andhigh signal in two-color Western blot detection.Proteins were separated in 4-20% Thermo ScientificPrecise Protein Gels and transferred to Low-Fluorescence PVDF Transfer Membrane (Product #22860). The membrane was blocked overnight in SEABLOCK Blocking Buffer (Product # 37527) and targetproteins were detected following the recommendedprotocol. Membranes were imaged with the LI-COROdyssey Infrared Imaging System. Tubulin wasdetected from the indicated quantity of HeLa cell lysate.Purified TNFα was detected at the indicated quantity.

Fluorescent Dye-Based Stains

Thermo Scientific Krypton Protein Stain† is a fluorescent stain for detecting proteins in sodiumdodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 2-D gels.

Highlights• Excitation/emission maxima – 520/580 nm• Compatibility – works with all SDS-polyacrylamide and 2-D gel types and with MS analysis • Linear quantitative range – three to four orders of magnitude• Sensitive – detects down to 0.25 ng protein with the basic 2.7-hour protocol • Fast – using the rapid protocol, detects down to 2 ng protein in 30 minutes• Comparative – minimal differential staining of proteins


Product # Description Pkg. Size Price46628 Krypton Protein Stain (10X) 20 ml $ 39

Sufficient reagent to stain four mini gels (8 cm x 10 cm)46629 Krypton Protein Stain (10X) 100 ml $110

Sufficient reagent to stain 20 mini gels (8 cm x 10 cm) or two to four large-format gels 46630 Krypton Protein Stain (10X) 500 ml $399

Sufficient reagent to stain 100 mini gels (8 cm x 10 cm) or 10 to 20 large-format gels† See patent information on inside back cover

Thermo Scientific Krypton Fluorescent Protein StainA faster, affordable fluorescent stain that provides excellent performance

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Pierce Protein Research ProductsTel: 815-968-0747 or 800-874-3723 • Fax: 815-968-7316 • Customer Assistance E-mail: [email protected]

Outside the United States, visit our website or call 815-968-0747 to locate your local branch office or distributor.© 2007 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Prices listed herein were accurate

at the time of printing. Visit our website for up-to-date prices. † U.S. patent pending on Dual-labeled Fluorescent Molecular Weight Marker Technology. U.S. patent pendingon Imperial Protein Stain Technology. U.S. patent pending on Krypton Infrared Protein Stain Technology. Alexa® is a trademark of Molecular Probes, Inc.

CyDye®, Cy® and Typhoon® are trademarks of GE Healthcare. Odyssey® is a trademark of LI-COR Biosciences.All other trademarks are property of Thermo Fisher Scientific Inc. and its subsidiaries.

www.thermo.com/dylight

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