formal diazo dye report
TRANSCRIPT
Synthesis of Methyl-Sudan I by Diazotization
Brooke Gushen
Introduction
Diazonium salts are very valuable reagents in many organic reactions because
diazotization reactions can be coupled with many other reaction pathways to yield stable
products with few side products. In particular, the Sandmeyer reaction is an example of
diazotization coupled with a multitude of nucleophilic substitutions.1 These reactions make it
possible to add protons, halogens, alcohols, and more onto an R-group, in particular onto
aromatic rings. Strong acids play a heavy role in the diazotization reaction, and there has been
research that has found that certain acids can affect the rate of the reaction. In a kinetic study of
diazotization run with sulfuric acid instead of hydrochloric acid, researchers found that sulfuric
acid can increase the rate of the reaction.2 So this is a very valuable reaction technique in organic
chemistry.
In this specific experiment, however, the diazotization reaction of an arylamine is
coupled with an electrophilic aromatic substitution, which yields a diazo dye called Methyl-
Sudan I. Azo and diazo compounds are very characteristic because they tend to be very brightly
colored compounds that are often used as dyes. Some of these dyes are used in liquid crystal
displays in televisions,3 some are found in textiles,4 and some have even been found in food. The
particular diazo dye synthesized in this experiment was at one point being used to brighten chili
powder in the UK.5 However, since these dyes were found to have carcinogenic qualities, they
have been banned from many uses.
The reaction mechanism for this experiment is a diazotization reaction coupled with an
electrophilic aromatic substitution.
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Figure 1. Reaction Mechanism of Diazotization.
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In this mechanism, the sodium hydroxide deprotonates the 2-naphthol yielding sodium 2-
naphtholate and water. The negative charge pushes to that particular carbon because it is more
stable at that adjacent carbon due to the resonance provided by the nearby aromatic ring. In
another reaction, the 4-methyl-aniline deprotonates the hydrochloric acid. The protonated aniline
is then deprotonated by the nitrous acid. An electron pair on the aniline’s nitrogen forms a bond
with the protonated nitrous acid, pushing the water leaving group off. The water molecule then
deprotonates the nitrogen, yielding a hydronium molecule. The aniline molecule then
tautomerizes to yield a double bond between the nitrogens. The hydronium molecule protonates
the –OH group on the aniline tautomer. The electron pair on the upper nitrogen then shifts down
to form a triple bond between the nitrogens and pushes off the water leaving group. When this
diazonium salt is added to the prepared sodium 2-naphtholate, the lone pair on the aromatic ring
forms a bond with the lower nitrogen and pushes a pair of electrons from the triple bond to the
upper nitrogen. A water molecule then comes in to deprotonate the molecule where the bond was
made with the nitrogen. This pushes the electrons to form a double bond on the aromatic ring,
giving the oxygen atom a negative charge. A hydronium molecule protonates this oxygen,
yielding an alcohol group on the aromatic ring. The final product is methyl-sudan I dye.
The purpose of this experiment was to synthesize a diazo dye from an arylamine
compound through a diazonium coupling reaction, which would also be purified by
recrystallization. 1H NMR spectroscopy and UV/Vis Spectrophotometry were used for further
analysis on the product of the diazotization. These methods evaluated the purity of the product.
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Experimental6
1. Methyl-Sudan I. 4-methyl-aniline (100 mg, 0.933 mmol), 12M hydrochloric acid (0.5 mL),
and distilled water (0.05 mL) were placed in a 10 mL flask equipped with a magnetic stir bar.
The reaction mixture was stirred and warmed until dissolved. It was then cooled in an ice bath to
0ºC. Sodium nitrite solution was prepared with sodium nitrite (80 mg, 1.16 mmol) and water (1
mL) in a reaction tube and was added drop-wise to the aniline solution. Five minutes after
addition was complete, the reaction mixture was tested for excess nitrous acid using starch iodine
paper. Urea crystals are added until excess nitrous acid was neutralized, as shown by the brown
color of the starch iodine paper. Sodium 2-naphtholate solution was prepared by adding 2-
naphthol (160 mg, 1.11 mmol) to 3M sodium hydroxide (1.5 mL) and heating slightly until
dissolved. This solution was then added in small amounts to the cooled diazonium solution while
stirring. After 15 minutes in the ice bath, the solution was isolated by vacuum filtration, and the
reddish orange crystals were washed with cold water (2 mL). The crude air-dried product (201
mg, 82.1% yield) was recrystallized from ethanol. Melting point was determined to be 120 to
134ºC (lit: 135ºC). 60 MHz 1H NMR, 400 MHz 1H NMR, and UV/Vis spectra were obtained for
the purified product, methyl-sudan I (88 mg, 35.9% recovery).
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1H-NMR Data: Obtained by using deuterated chloroform solvent on a 400 MHz NMR.
Significant Peaks
Observed Splitting (Integral Value), Type of Hydrogen
2.4128 ppm S (3.037), CH3
6.9114 ppm D (0.999), H of Aromatic Ring
7.2740 ppm D (2.020), H of Aromatic Ring
7.3699 ppm T (1.032), H of Aromatic Ring
7.5367 ppm T (1.049), H of Aromatic Ring
7.6154 ppm D (1.039), H of Aromatic Ring
7.6633 ppm D (2.032), H of Aromatic Ring
7.7132 ppm D (1.038), H of Aromatic Ring
8.5955 ppm D (1.008), H of Aromatic Ring
16.1942 ppm S (0.958), O-H
UV-Vis Data: Ran on dilute solution of Methyl-Sudan I in methanol on UV/Vis spectrophotometer.
Wavelength (nm) Color Absorbed Observed Color Absorbance (AU)
420.0 Violet-Blue Yellow-Orange 0.49611
482.0 Blue-Green Red 0.53729
Results and Discussion
In this experiment, the 12M HCl was added to the aniline to be sure that all the molecules
were protonated. This protonated form could then easily transfer its proton to the nitrous acid,
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giving it a better leaving group. The nitrous acid was produced after a proton transfer occurred
between the sodium nitrite and water. There are many proton transfers that occur between the
aniline molecule and water, allowing it to eventually become a diazonium salt, which is why the
presence of water is so important. The urea was added to oxidize any remaining nitrous acid in
the solution because if the nitrous acid were present with the sodium 2-naphtholate, it would just
revert it back to 2-naphthol. When the sodium 2-naphtholate is added to the diazonium salt, the
sodium 2-naphtholate acted as the nucleophile and the diazonium salt as the electrophile creating
the nitrogen bond. Further proton transfers between the molecule and water eventually yielded
the crude product. An 1H MNR spectrum of the crude product showed presence of impurities.
The methyl-sudan I was recrystallized in ethanol to get rid of any side products that may have
formed during the synthesis. Ethanol is used because it has similar polarity to the product.
In Figure 3, the UV/Vis shows absorbance peaks at 420.0 and 482.0 nm. At 420.0 nm, the
absorbance peak means that light was absorbed at the violet-blue part of the visible light
spectrum. Because violet-blue wavelengths were absorbed, the complementary color yellow-
orange was reflected in the diazo dye. At 482.0 nm, the absorbance peak means that light was
absorbed at the blue-green part of the visible light spectrum. Because blue-green wavelengths
were absorbed, the complementary color red was reflected in the diazo dye. This proves true in
the reddish orange color the dye appears after synthesis and purification was completed.
In Figures 1 and 2, the 1H-NMR of methyl-sudan I in deuterated chloroform produced
peaks in ten distinct groupings. The first peak at 2.4128 ppm showed the splitting pattern of
Protons A, a singlet with an integral value of 3.037 because it had three chemically equivalent
protons with no adjacent protons. These protons are found at the lower end of the spectrum
because they are bonded to a methyl group. The peaks at 6.9114, 7.6154, and 7.7132 ppm
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showed the splitting pattern of Protons F, G, and K, a doublet with an integral value around
1.000 because each was adjacent to one other proton. It cannot be determined exactly which
proton goes with each doublet however. The peaks at 7.2740 and 7.6633 ppm showed the
splitting pattern of Protons B and C, a doublet with an integral value around 2.000 because each
had two chemically equivalent protons adjacent to one other proton. It cannot be determined
exactly which proton goes with each doublet however. The peaks at 7.3699 and 7.5367 ppm
showed the splitting pattern of Protons H and J, a triplet with an integral value around 1.000
because each was adjacent to two other protons. It cannot be determined exactly which proton
goes with each doublet however. The peak at 8.5955 ppm showed the splitting pattern of Proton
E, a doublet with an integral value around 1.000 because it was adjacent to one other proton. Its
chemical shift was pulled further towards 8-9 ppm because it is more deshielded due to its
proximity to the alcohol group. All the peaks from 6.9114 to 8.6166 ppm are protons bonded to
aromatic rings. The last peak at 16.1942 ppm showed the splitting pattern of Proton D, a singlet
with an integral value of 0.958 because it has no adjacent protons. This proton is found to the
higher end of the spectrum because it is directly bound to an oxygen atom, which deshields the
proton.
The melting point was expected to be around 135ºC. The melting point range was found
to be 120 to 134ºC. This shows that there may have been very trace amounts of impurity present
to cause the solid dye to melt initially at such a low temperature, however, such impurities
should have been evident on the NMR spectra. The upper end of the range comes very close to
the expected melting point, which suggests that the product was indeed in its pure form.
The percent yield was 82.1% before recrystallization. This high percent yield may have
been due to the fact that highly concentrated hydrochloric acid effectively protonated all the p-
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toluidine. Also, the urea was used to oxidize any excess nitrous acid, and the produced crystals
were washed during vacuum filtration. All of these steps effectively bind up side products and
rid the product of most impurities. Since there still appeared to be impurities from an NMR
spectrum, the product was recrystallized. The percent recovery was found to be 35.9%. The
percent recovery is much lower because some of the crude crystals were used in NMR and
melting point analysis. Also, much of the diazo dye crystals were lost in transfer from flask to
watchglass.
Overall synthesis of a pure diazo dye, methyl-sudan I, was successful by a diazotization
reaction. The 1H NMR and UV/Vis spectra supported the predicted results.
References
1. McMurry, J. Organic Chemistry, 7th ed. 2008: Brooks/Cole, pp. 941-945.2. Aboul-Seoud, A. A Kinetic Study of the Diazotization of Aniline in Dilute Sulphuric Acid.
Bulletin des Sociétés Chimiques Belges, 1966, 75, 599.3. Chigrinov, V.; Prudnikova, E.; Kozenkov, V.; Kwok, H. Synthesis and Properties of Azo
Dye Aligning Layers for Liquid Crystal Cells. Liquid Crystals, 2002, 29, 1321.4. Flintoff, R.J.; On the Relative Importance of the Stability of Diazo Compounds to Practical
Utility in the Production of Insoluble Oxy-Azo Colours on Cotton Cloth. The Journal of the Society of Dyers and Colourists, 1902, 18, 96.
5. (author unknown). Sudan Outraged at Namesake Dye, British Broadcasting Company, 2005.6. Williamson, K. L. Macroscale and Microscale Organic Experiments, 2nd ed. 1994: Houghton
Mifflin, pp 1-3.
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