1. http://www.salon.com From student project to marketplace: Rapid Pathogen Screener for Food Safety Testing Monika Weber Yale University 1

2. Professor Hur Koser and Dr. Muhammet Uncuer MEMS Design Class Class Room 2 Homework Assignment Design a MEMS device with market potential Memx.com 3. How to get started? Hat is the problem we want to solve? Class Room 3 4. Food Safety Testing Liquid products Water Safety Monitoring Drinking water Recreational Water Safety Health and Safety Antibiotic resistance Food Safety Monitoring Problem: outbreaks of bacterial infections are hard to diagnose http://www.salon.com Fast Bacteria detection is important! Outbreaks reoccur 4 5. Current testing methods long and expensive Standard Plate Count Culture samples and manual count of aerobic bacteria Time: 24 - 48 hr Cost: ~$20 / test Set-up: Lab, incubator, labor Accuracy: More samples, longer incubation time PCR Enzymatic-based replication of DNA, and readout of fluorescent signal Time: 2 - 3 hr Cost: ~ $50 / test Set-up: PCR machine, labor Accuracy: More samples, longer incubation time http://app.infoaa.7700.gnb.ca/gnb/Pub/EServices/ListServiceDetails.asp 5 6. 6 Problem We have a problem, How to solve it? 7. Target specification: Miniaturized High accuracy Portable High sensitivity No culture growth Short detection time Low cost Easy in operation Target Specification: Less that 10 bacteria in 100ml of water detected faster than 30min Rapid Pathogen Screener Our solution 7 8. 8 Solution How to make the device? 9. Bacteria Separation 9 10. Dielectrophoresis 10 Target sensitivity: 10 bacteria in 1cc animal blood sample Dielectrophoretic force confines bacteria within a defined region of the channel Size (R) and dielectric properties () based separation Different species have different , changing the permittivity of the fluid affects separation 11. Bacteria Separation 11 DEP force simulation COMSOL multiphysics Microchannel cross section Dielectrophoretic force on EColi 12. STEP 1 Device uses RF electric field to manipulate and condense E.coli bacteria in water STEP 2 Device uses antibodies and nanowire sensors to detect E.coli bacteria E.Coli trapped in electric field Bacteria detection method 23 ))((2 EKrealrF mDEP 12 13. 13 Solution This is the device 14. 14 Screen device design 15. 15 Screen device prototype 16. Proof of concept demonstrated Prototype under development Screen device prototype 16 17. Prof. Mark Reed supports prototyping in Reedlab at Yale University Thanks to co-workers Brian Goldstein, Shari Yosinski, Sonya Sawtelle, Claudia Shin, Phillip McCown, Shivang Patel, Luye Mu, Christopher Yerino, Hazael Montanaro, Kane Siu Lung Lo Screen was initially designed as a project in Prof. Kosers class at Yale University We thank HDTRA for support Awards and Acknowledgements Grand Prize in the NASA Create the Future Design Contest 2011 CIMIT Primary Care Competition (3rd place US Universities) 2012 Sobotka Research Award 2011 17 18. Thanks to my team! 18 Shari Yosinski, Sonya Sawtelle, Claudia Shin, Brian Goldstein, Christopher Yerino, Hazael Montanaro, Kane Siu Lung Lo and Prof. Mark Reed