fss-i 3 dna expert system suite. dna profiling sample e.g. hair, skin
TRANSCRIPT
FSS-i3
DNA expert system suite
DNA Profiling
• Sample e.g. hair, skin
DNA Profiling
• Sample e.g. hair, skin
• Process sample to obtain raw data
DNA Profiling
• Sample e.g. hair, skin
• Process sample to obtain raw data
• Produce a profile of the DNA
DNA Profiling
• Sample e.g. hair, skin
• Process sample to obtain raw data
• Produce a profile of the DNA
• Store profile in NDIS (National DNA Index System) or match to existing profile
Expert System profiling motivation
• Sample & data processing already heavily automated
Expert System profiling motivation
• Sample & data processing already heavily automated
• Sample data analysis; DNA profiling is the bottle neck.
Expert System profiling motivation
• Sample & data processing already heavily automated
• Sample data analysis; DNA profiling is the bottle neck.
• Two independent data reviews required to upload a profile to NDIS
Expert System profiling motivation
• Sample & data processing already heavily automated
• Sample data analysis; DNA profiling is the bottle neck.
• Two independent data reviews required to upload a profile to NDIS
• Save enormous amounts of review time
FSS-i3 Expert System
• FSS: Forensic Science Service of the UK
FSS-i3 Expert System
• FSS: Forensic Science Service of the UK• Developed a software suit called FSS-i3
– 6 years of R&D and validation
FSS-i3 Expert System
• FSS: Forensic Science Service of the UK• Developed a software suit called FSS-i3
– 6 years of R&D and validation
• Designed by forensic scientists for forensic scientists
FSS-i3 Expert System
• FSS: Forensic Science Service of the UK• Developed a software suit called FSS-i3
– 6 years of R&D and validation
• Designed by forensic scientists for forensic scientists
• Made available to forensic community– USA, Canada, Europe, Australia, New Zealand
FSS-i3 Expert System
• FSS: Forensic Science Service of the UK• Developed a software suit called FSS-i3
– 6 years of R&D and validation
• Designed by forensic scientists for forensic scientists
• Made available to forensic community– USA, Canada, Europe, Australia, New Zealand
• Full Audit system
i-STRess
• The core DNA interpretation tool
i-STRess
• The core DNA interpretation tool
• Produces single source DNA profiles using configurable rules & filters
i-STRess
• The core DNA interpretation tool
• Produces single source DNA profiles using configurable rules & filters
• Detect multi-person DNA mixtures within a profile & export to i-STReam…
i-STReam
• Unique functionality to resolve two person DNA mixtures
i-STReam
• Unique functionality to resolve two person DNA mixtures
• Allows previously unusable DNA profiles to be uploaded to DNA databases
i-ntegrity
• DNA samples/data can become contaminated: accidental DNA mixture
i-ntegrity
• DNA samples/data can become contaminated: accidental DNA mixture
• System flags possible profile contaminations for further review
i-ntegrity
• DNA samples/data can become contaminated: accidental DNA mixture
• System flags possible profile contaminations for further review
• Saves time: no more manual reviews of profiles
FSS-i3 Validation
• FSS-i3 developed from internal FSS expert systems– 6 years of testing
FSS-i3 Validation
• FSS-i3 developed from internal FSS expert systems– 6 years of testing
• 1 million+ profiles uploaded to UK National DNA DB
FSS-i3 Validation
• FSS-i3 developed from internal FSS expert systems– 6 years of testing
• 1 million+ profiles uploaded to UK National DNA DB
• FSS-i3 validation project:– 70 000 previously analyzed samples– Minor discrepancies in 0.03% of samples & each of these
samples were flagged by the system for further review
Technology used in FSS-i3
• Strategies for implementing rules: unknown
Technology used in FSS-i3
• Strategies for implementing rules: unknown
• User optimization: 20+ rules settings
Technology used in FSS-i3
• Strategies for implementing rules: unknown
• User optimization: 20+ rules settings
• Designed to run on stand-alone Pentium 3 PC’s
Design & Development of FSS-i3
• Development time: six years
Design & Development of FSS-i3
• Development time: six years
• “The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face” (1)
Design & Development of FSS-i3
• Development time: six years
• “The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face” (1)
• “i-STReam is based on scientific theory developed by Dr Peter Gill and Dr Tim Clayton” (1)
Conclusions: FSS-i3
• Consistent profiling of samples
Conclusions: FSS-i3
• Consistent profiling of samples
• Ability to interpret mixed DNA profiles
Conclusions: FSS-i3
• Consistent profiling of samples
• Ability to interpret mixed DNA profiles
• Check for contamination
Conclusions: FSS-i3
• Consistent profiling of samples
• Ability to interpret mixed DNA profiles
• Check for contamination
• Significant reduction in time spent on the above, due to automation
References
• (1) http://www.forensic.gov.uk/forensic_t/i3/index.htm
• (2) http://www.promega.com/profiles/802/profilesindna_802_08.pdf
• (3) http://forensics.marshall.edu/NEST/Nest%20PDFs/Documents/NIJEvalExpertSys.pdf
• (4) http://www.promega.com/profiles/1001/profilesindna_1001_11.pdf
• (5) http://www.kathyreichs.com/dnaprofiling.htm
• (6) http://www.biotechnologyonline.gov.au/human/dnaprofile.cfm