functional and structural human research centre

Functional and Structural

Human Research Centre

Laboratories:

• Exercise Biochemistry

• Physiology

• Genetics

• Kinesiology

Our main goal is to professionally support

the process of training and to combine sports and science

Our capacities are proven by a long-term cooperation with athletes and sports clubs.

We work with young people, amateurs,

professionals, country representatives and even

Olympic champions.

Physiology

Laboratory

Research facilitiesThe Laboratory is fully equipped, including:

Sport treadmills that can interface withmetabolic and ECG systems, thusproviding a fully integratedcardiopulmonary exercise testing systemwith different configurations andspecifications to fit any request for clinicalor sport testing.


Research grade stationary system for accurate and reliable metabolic measurements.State-of-the-art breath by breath gasexchange data analysis (VO2, VCO2), fastresponse paramagnetic O2 sensor.Fully integrated 12-lead ECG for Stress Testing.Full spirometry and exercise SpO2 monitoring.

Portable system for cardiopulmonary gasexchange analysis on true breath-by-breathbasis. Its technology and dimensions allowit to measure physiological response toexercise in the field without limitation.


Patent Reactive Grid, Cutting andSerpentine drills push athletes totheir limits of:- reaction time and first movement,- reactive change of direction andagility,- peripheral vision,- decision making.


Simple yet powerful jump monitoring system:Vertical jump testing remains one of the mostwidely use methods for testing and trainingpower and strength in a wide range of sports.Every jump is individually measured and a rangeof variables generated automatically:- contact time, flight time, reactive strengthindex, average power and impulse,- use power measurements to monitor recovery,training response and power gains.


Kinesiology

Laboratory

Noraxon’s 3-D MyoMotion system that is intended to measure and quantify angular changes of selected body segments. It is a part of a unique multi-device concept called Noraxon MyoMotion lab where other biomechanical technologies such as telemetric EMG, pressure plates/soles, force plates, video and HS video and other devices can directly be added via plug & play technology.


Sports and orthopedic medicine,pediatric medicine, neurorehabilitation,older adult medicine, industrialmedicine, and researchers depend onBiodex to provide consistent, accurate,objective data.Objective data that provides the bestoutcome for their patientsobjective data that supports theirresearch objective data that separatestheir facility from the rest.


Catapult system, which is the mostcomprehensive solution for the world game: aGNSS-enabled monitor which doubles thesatellites to double the accuracy, a wirelesslocal positioning system to capture movementinside and out, and IMA for micro-movementmetrics.We’ve been independently validated over 60times – in every side-by-side comparison.


Exercise Biochemistry

Laboratory

Research facilities

The Laboratory owns standard equipment needed fortypical biochemical and hematological diagnostic assays.

Automatic clinical chemistry randomaccess analyzer (BM100) allowing toanalyze enzyme activities (CK, CKMB, AST,ALT, ASP, GGT, LDH and others), theassessment of blood metabolites levelsincluding glucose, bilirubin, uric acid, urea,creatinine, C-reactive protein, totalprotein, albumin.

Ion Selective Analyzer allowing to assesblood electrolyte levels (Na+, K+, Cl-,HCO3

-) as well as blood acidification.

Research facilities

Haematology Analyzer allowing to preform full 18-parameter blood morphologyincluding:RBC, WBC, HGB, HCT, MCV, MCH, MCHC, PLT, PCT, MPV, PDW, RDW,LYM (% and #), MON (% and #),GRAN (% and #), histograms: WBC, RBC, and PLT.

Research facilities

Synergy H1 Hybrid Multi-Mode Microplate Reader

This system supports top and bottom fluorescence intensity, UV-visible absorbance andhigh performance luminescence detection.

It is the ideal system for all the standard microplateapplications , including the analysis of concentrations of :- hormones (e.g. TSH, FT3, FT4, FSH, terstosterone,

estradiol, parathyroid hormone),- Proteins (e.g. ceruloplasmin, ferritin, transferrin,

hemoglobin),- pro- and antiinflammatory cytokines, e.g. TNF, IL-6, IL-2,- ions, e.g. iron, magnesium, phosphate,- selected vitamins,- ATP, etc.

Research facilities

1-D and 2-D protein electrophoresis equipment, Semi-Dryand Rapid Blotting Systems for qualitative and quantitativeassessment of such proteins as HIV-1, procaspases, activecaspases, regulatory proteins (e.g. VEGF) and transcriptionfactors (e.g. NF-B), cytokines as well as chemokines (e.g. IL-1, IL-1, IL 8).


The High Performance Liquid Chromatography (HPLC)system with integrated diode-array detector and massspectrometer (SQ-MS) for proven quality and highperformance analyses of various metabolites connectedwith training and exercise.


BD Accuri™ C6 Cytometer equipped with a blue and a red laser, two light scatterdetectors, and four fluorescence detectors (configurable for 4 blue, 3 blue + 1 red or2 blue + 2 red lasers).

It allows to analyze human peripheral bloodmononuclear cells to asses e.g. expression ofcell cytokines, cell membrane receptors,immune response to the training exercise,different aspect of cell signaling and celldeath as a result of the exercise.


Genetics

Laboratory

• 4-capillary 3130 Genetic Analyzer is a complete system optimized for multiple applications.

• This is versatile platform that has been designedfor easy conversion between applications with minimal intervention of the user.

• Reproducible results can be achieved from both sequencing and fragment analysis chemistries for a wide variety of applications in sportsgenomics:

• de novo sequencing; • comparative sequencing; • genome finishing and contig assembly; • construct validation; • large template sequencing; • microsatellite analysis; • SNP discovery, validation, and screening.


CFX96 Touch Real-Time PCR Detection System


• Real-time PCR detection system is six-channeldevice that combines advanced opticaltechnology with precise thermal control to deliversensitive, reliable detection of wide variety ofgenetic polymorphisms analysed in sportsgenetics.• The system is designed to advance geneexpression analysis by relative quantity ornormalized expression with multiple referencegenes studied in exercise and training researchprogrammes.

Agilent 2100 Bioanalyzer

• Platform for DNA, RNA, protein and cell analysis• The Agilent 2100 bioanalyzer offers a broad

range of pre-validated analysis kits combined with an easy-to-use benchtop system

• On-chip flow cytometry - the easy way to acquire dual-color, cell-based fluorescence data

• RNA quality check with RIN - the industry standard for RNA analysis offering total RNA, mRNA and Small RNA’s data including RIN algorithm (RNA Integrity Number)

• DNA size and quantity - smart, high resolution separation and quantification of DNA

• SDS-PAGE replacement for Protein analysis - the fast and reliable way to determine the quantity and purity of proteins from Coomassie down to silver stain sensitivity


Horizontal and vertical electrophoresis systems

• The instruments comprise a comprehensiveand versatile gel electrophoresis systems thateffectively separates nucleic acids (RNA andDNA) using agarose as well as polyacrylamidegels.• The system is ideal for the separation of DNArestriction digestions, Polymerase ChainReaction amplified fragments, and genomicDNA and RNA prior to Southern or Northernblotting.• The system contains Semi-Dry and RapidBlotting devices for qualitative and quantitativeassessment of nucleic acids.


Thank you

functional and structural human research centre

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