Please refer disclaimer Overleaf.

M432Furunculosis Agar

Ingredients Gms / LitreTryptone 10.000Yeast extract 5.000Tyrosine 1.000Sodium chloride 2.500Agar 15.000**Formula adjusted, standardized to suit performance parameters

DirectionsSuspend 33.5 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. DO NOT OVERHEAT. Allow the tubes to cool at 45-50ºC in slanted position.

Principle And InterpretationAeromonas are ubiquitous inhabitants of natural waters, both fresh and salt where they infect animals, including amphibians, reptiles and fish. In human, they are most commonly associated with infections of wounds acquired near or in water, or with diarrhoeal diseases. The fish pathogen Aeromonas salmonicida prefers temperature of 23°C for their growth, thus it is least likely to cause human infections. A. salmonicida is the causative agent of furunculosis (6), a disease of major significance in the culture of salmonid fish (1). The disease represents a serious problem to farming of Atlantic salmon and causes extensive economic losses to freshwater hatcheries and sea farms. The absence of an efficient selective medium and the poor plating efficiency of the organism in mixed cultures (5) have hampered the development of an efficient diagnostic test for Aeromonas salmonicida and, consequently, the control of furunculosis in salmonid culture. Furunculosis Agar is formulated as per Griffin et al (2) for detection of Aeromonas salmonicida (salmonids-furunculosis) on the basis of production of brownish red pigment. The medium contains tryptone; tyrosine and yeast extract which are sources of carbon, nitrogen, vitamins and minerals. Sodium chloride provides essential ions. Brownish red pigmentation of the colonies in the medium within two to three days of incubation at 22°C is positive presumptive evidence.

Intended Use:Recommended for detection of Aeromonas salmonicida by means of its brownish red pigment production. Composition**

Type of specimen Water samples

For the more rapid presumptive test, 0.5 ml of 1% aqueous solution of paraphenylenediamine can be applied to the colonies of a 24 hours old culture growing on the surface of the agar slants. Contact the reagent with all the growth. After application of the reagent, the tubes should be tipped and rotated to spread the reagent to cover the growth on slant, a deep purple colour is seen within 45 to 90 seconds.

Specimen Collection and Handling:

Warning and Precautions :Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations :1. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.

Performance and EvaluationPerformance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

2.Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user’s unique requirement.

HiMedia Laboratories Technical Data

Aeromonas salmonicida ATCC 33658

50-100 brownish red

Reference

6. Popoff M., Genus III, Aeromonas Kluyver and Van Niel, 1936, 398 AL,p. 545-548. In Krieg N. R. and Holt J. G., (Eds.), 1984, Bergeys Manual of Systematic Bacteriology, Vol. 1, Williams & Wilkins Co., Baltimore.

1. Austin B. and Austin D. A., 1987, Bacterial fish pathogens: diseasein farmed and wild fish, p. 112-117. Ellis Horwood Ltd., Chichester, United Kingdom.

5. McCarthy D. H., 1977, Soc. Appl. Bacteriol. Symp. Ser., 6:229.

2. Griffin, Snieszko and Friddle, 1953, Vet. Med., 48:280.

Revision : 02/2021

Disclaimer :

User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm,comparable with 1.5% Agar gelColour and Clarity of prepared mediumLight amber coloured, clear to slightly opalescent gel forms in tubes as slants Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-48 hours.

Organism Inoculum(CFU)

Colour ofcolony

Storage and Shelf LifeStore between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).

Disposal

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3.4. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)

Manual of Clinical Microbiology, 11th Edition. Vol. 1.

Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.