fusion cell gene profiling identify mbo and cg15252 enhancer molecular mechanism of target gene...
Post on 15-Jan-2016
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![Page 1: Fusion cell gene profiling Identify mbo and CG15252 enhancer Molecular mechanism of target gene regulation by Dys::Tgo Dys::Tgo activation of CG13196 Dys:Tgo](https://reader035.vdocument.in/reader035/viewer/2022081603/56649d4a5503460f94a270bd/html5/thumbnails/1.jpg)
•Fusion cell gene profiling
•Identify mbo and CG15252 enhancer
•Molecular mechanism of target gene regulation by Dys::Tgo
•Dys::Tgo activation of CG13196
•Dys:Tgo activation of CG15252
•Identify dys fusion cell enhancer
dys-gal4::UAS-CD8-GFP btl-gal4::UAS-CD8-GFP
dys-gal4::UAS-CD8-GFP dys-gal4::UAS-CD8-GFP ; dys/dys
•Identify dys target gene
![Page 2: Fusion cell gene profiling Identify mbo and CG15252 enhancer Molecular mechanism of target gene regulation by Dys::Tgo Dys::Tgo activation of CG13196 Dys:Tgo](https://reader035.vdocument.in/reader035/viewer/2022081603/56649d4a5503460f94a270bd/html5/thumbnails/2.jpg)
dys genomic structure
FTF Q72L FTF T31N
F2F elution
LP05454
Exon1 2 3 4 5 6 7 8 9 10
1.7kb2.7kb
3.8kb
3.8kb
3.8kb
![Page 3: Fusion cell gene profiling Identify mbo and CG15252 enhancer Molecular mechanism of target gene regulation by Dys::Tgo Dys::Tgo activation of CG13196 Dys:Tgo](https://reader035.vdocument.in/reader035/viewer/2022081603/56649d4a5503460f94a270bd/html5/thumbnails/3.jpg)
CG13196 enhancer
![Page 4: Fusion cell gene profiling Identify mbo and CG15252 enhancer Molecular mechanism of target gene regulation by Dys::Tgo Dys::Tgo activation of CG13196 Dys:Tgo](https://reader035.vdocument.in/reader035/viewer/2022081603/56649d4a5503460f94a270bd/html5/thumbnails/4.jpg)
Expression patterns of CG13196 enhancer and TCGTG enhancer
Early S15
4xTCGTG GFP Dys
S16
CG13196 GFP Dys
![Page 5: Fusion cell gene profiling Identify mbo and CG15252 enhancer Molecular mechanism of target gene regulation by Dys::Tgo Dys::Tgo activation of CG13196 Dys:Tgo](https://reader035.vdocument.in/reader035/viewer/2022081603/56649d4a5503460f94a270bd/html5/thumbnails/5.jpg)
CG13196 4xTCGTG GFP
S15
S16
Dys Dys
Ectopic expression of dys can activate CG13196 and TCGTG enhancer ectopically
![Page 6: Fusion cell gene profiling Identify mbo and CG15252 enhancer Molecular mechanism of target gene regulation by Dys::Tgo Dys::Tgo activation of CG13196 Dys:Tgo](https://reader035.vdocument.in/reader035/viewer/2022081603/56649d4a5503460f94a270bd/html5/thumbnails/6.jpg)
CG15252 5’ upstream
Exon1
3.2kb
9.0 kB 5’ upstream
0.8 kb TCGTG cluster
TCGTG TCGTG TCGTG
![Page 7: Fusion cell gene profiling Identify mbo and CG15252 enhancer Molecular mechanism of target gene regulation by Dys::Tgo Dys::Tgo activation of CG13196 Dys:Tgo](https://reader035.vdocument.in/reader035/viewer/2022081603/56649d4a5503460f94a270bd/html5/thumbnails/7.jpg)
Spalt is not required for CG15252 activation by dys in DT
Need to do:CG15252 expression in Spalt mutants
CG15252 Dys CG15252 Dys
btl-gal4;UAS-dys btl-gal4;UAS-spalt;UAS-dys
![Page 8: Fusion cell gene profiling Identify mbo and CG15252 enhancer Molecular mechanism of target gene regulation by Dys::Tgo Dys::Tgo activation of CG13196 Dys:Tgo](https://reader035.vdocument.in/reader035/viewer/2022081603/56649d4a5503460f94a270bd/html5/thumbnails/8.jpg)
Ectopic dys can activate mbo-lacZ ectopically
mbo-lacZ-Dys
btl-gal4, UAS-dys
![Page 9: Fusion cell gene profiling Identify mbo and CG15252 enhancer Molecular mechanism of target gene regulation by Dys::Tgo Dys::Tgo activation of CG13196 Dys:Tgo](https://reader035.vdocument.in/reader035/viewer/2022081603/56649d4a5503460f94a270bd/html5/thumbnails/9.jpg)
mbo 3’
TCGTG
407bp
ACGTG
![Page 10: Fusion cell gene profiling Identify mbo and CG15252 enhancer Molecular mechanism of target gene regulation by Dys::Tgo Dys::Tgo activation of CG13196 Dys:Tgo](https://reader035.vdocument.in/reader035/viewer/2022081603/56649d4a5503460f94a270bd/html5/thumbnails/10.jpg)
Conclusion
•There are other transcriptional activators turns on at late stages or changes in chromatin structure.
• Spalt is not required for CG15252 expression
•There is no transcriptional inhibition at early stage
• dys target genes can only be turn on at late stages (late 14 or early 15)