gene expression *protein coding gene *gene expression genes control inherited variation via: dna,...
TRANSCRIPT
Gene Expression
*Protein coding gene
*Gene expression
Genes control inherited variation via:
DNA, RNA and protein
Phenotype
*Gene
DNA PhenotypeRNA ProteinReplication TranscriptionTranslation
Brief overview of transcription:
Transcription*RNA polymerase (from E. coli )
2 ’
CoreSigma factor
Holoenzyme
Transcription
Functions of a prokaryotic promoter:
1. ON/OFF Switch
2. Speed Switch
3. Alignment
Binding of RNA Polymerase to Promoters
How tightly does core enzyme vs. holoenzyme bind DNA?
Transcription Initiation
Transcriptional Elongation:
Termination of TranscriptionInverted Repeats and Hairpins
• The repeat at right is symmetrical around its center shown with a dot
• A transcript of this sequence is self-complementary– Bases can pair up
to form a hairpin as seen in the lower panel
Termination of Transcription
*Rho-independent termination
factor
Termination of Transcription*Rho-dependent termination