general presentation - alexandru ioan cuza university · general presentation ... tp 1020; -leica...

General presentation

Research team

Research directions Infrastructure

Assoc. Prof. PhD Ionel

MIRON Email:

[email protected]

Lecturer PhD Anca-

Narcisa NEAGU

Email: [email protected]

Research Assist. PhD

Carmen AONCIOAIE

Email:

[email protected]

om

Research Assist. Mihai

Georgel ERHAN

Email:

[email protected]


Aliona NOVAC

Email:

[email protected]


Maria Magdalena Dascălu,

Email:

[email protected]

-Assessement of reservoirs water quality

using the bioindicators: phytoplankton,

chlorophyll a, zooplankton,

macrozoobenthos and ichtyofauna;

-Impact of intesive trout aquaculture on the

water quality of reservoirs;

-Ecomorfological studies on the

poluosensitive structures of bioindicators.

The Laboratory has initiated the procedures

for obtaining the accreditation from

Romanian Accreditation Association

RENAR, according to the Romanian

standard ISO 17025/2005.

The aim of this process is to certify the

analysis: assessment of reservoir water

quality using:

- the phytoplankton,

-the chlorophyll a,

-the zooplankton,

-the zoobenthos

-the ichtyofauna.

-Reservoir research boat;

-Multiparameter Water Quality

Sonde YSI 6600V2-2;

-Scientific diving equipment Coltri

Sub;

-Spectrophotometer UV VIS

Shimadzu 1700;

-LASER Confocal Scanning

Microscope Leica DM 5500Q

TCS SPE with DFC 290 camera;

-Inverted microscope Nikon

Eclipse TS 100;

-Leica Cryostat CM 1850;

-Leica Rotary Microtome RM

2265;

-Leica Automatic Tissue Processor

TP 1020;

-Leica Autostainer XL (ST 5010);

-Embedding Station Leica EG

1150;

-Leica EM AMW – Automatic

Microwave Tissue Processor for

electron Microscopy;

-EMS 850 Critical Point Drier;

-EMS 550X Sputter Coater.

“ALEXANDRU IOAN

CUZA” UNIVERSITY OF

IASI

ROMANIA

LABORATORY FOR

ASSESSING WATER

QUALITY USING

BIOINDICATORS

FACULTY OF

BIOLOGY

mailto:[email protected]







Research team

Echipa de cercetare

Domenii de

competenţa

Lecturer PhD Anca-

Narcisa NEAGU

Technical Manager

e- mail: [email protected] tel +40232 201667

Animal ecomorphology

Animal histology

Animal embriology

Optic and electron

microscopy

Bioindicators of water

quality

Assoc. Prof. PhD Ionel

MIRON

Quality Manager

e-mail: [email protected] tel.+40232 201504

Limnology

Aquaculture

Ichthyology

Bioindicators of water

quality


Carmen AONCIOAIE

Email:

carmensilva2003@yahoo

.com

Plant taxonomy

Aquatic ecology

Phytoplankton

Research Assist. Mihai

Georgel ERHAN

Email:

[email protected]

Aquatic ecology

Zooplankton (Copepoda,

Cladocera)


Aliona NOVAC

Email:

[email protected]

Aquatic ecology

Ichthyology

Oligochaeta


Maria Magdalena

Dascălu,

Email:

[email protected]

Entomology

Chironomids








Research projects

Research projects Characteristics Budget

(RON)

1. EXTENSION OF THE

AQUATIC ECOLOGY AND

AQUACULTURE

LABORATORY OF POTOCI

FOR ASSESSING THE

WATER POLLUTION

INDUCED BY SALMONID

FLOATING FARMS

ACCORDING TO THE EU

WATER FRAMEWORK

DIRECTIVE 60/2000.

205/2006-2008, Director Lecturer PhD NEAGU

Anca-Narcisa, CEEX, M IV, RENAR

WEB site:

www. bio.uaic.ro/cercetare/contracte/acvapur/acvapur-1-ro.pdf

www. bio.uaic.ro/cercetare/contracte/acvapur/acvapur-1-en.pdf

799 370

2. EXTENSION OF THE

INFRASTRUCTURE OF THE

LABORATORY OF

BIOINDICATORS OF WATER

QUALITY – ACVAPUR

POTOCI, IN ORDER TO

ASSESS THE

POLLUOSENSITIVITY OF

AQUATIC ORGANISMS.

PN II, Capacities, M I, nr. 93/2007-2009,

Director Lecturer PhD NEAGU Anca-Narcisa,

ANCS.

WEB site:



1 729 510

3. RESEARCHES ON THE

STRUCTURE AND

FUNCTIONING OF A

LACUSTRIAN ECOSYSTEM

FORMED BY A RIVER –

LAKE ECOLOGICAL

SUCCESSION.

PN II, Idei 434/2007-2010, Director

Professor PhD Ionel Miron, UEFISCSU.

WEB site:



1 000 000

EXTENSION OF THE INFRASTRUCTURE OF THE

LABORATORY OF BIOINDICATORS OF WATER QUALITY –

ACVAPUR POTOCI, IN ORDER TO ASSESS THE

POLLUOSENSITIVITY OF AQUATIC

ORGANISMS

The main aim of this project consists in extending the capacity of the Laboratory of the

bioindicators of the water quality - ACVAPUR, in order to demonstrate the particularities of

some aquatic taxa of being polluosensitive, to make them new water quality bioindicators.

As it stands now, the ACVAPUR Laboratory is specialized on monitoring the water

quality by biological methods based on bioindicator taxa (phytoplankton, zooplankton,

macrozoobenthos and ichthyofauna), as a means of analyzing the impact of the intensive salmon

farming in floating cages on the aquatic communities and on the water quality of Bicaz

Reservoir. By this project, we intend to extend the capacity of this laboratory by implementing new

biological technologies for the detection of the pollutants and their impact on the morphology

and the structure of the aquatic polluosensitive taxa, as well as on polluosensitivity mechanisms.

The biological methods for assessing the water quality have multiple advantages over

the classical physico – chemical methods. These are less expensive and are based on the some

taxa to be sensitive or tolerant to the different types of pollution.

Also, by their bioaccumulation capacity, aquatic organisms can deposit the pollutants in

their organs and tissues, realizing a great concentration of pollutants in “target structures” and

this can allow us to detect the low levels of aquatic pollutants, even after the pollution wave

disappeared. The bioaccumulation mechanisms are studied in large number of countries, and

they form the basis of some new methods for assessing and monitoring the water quality.

Our project answers the requirements of 60/2000 Water Framework Directive, that

recommends the classification of the water in 5 quality categories, taking into account living

organisms, which is reflected by the structure and functioning of the organism communities

living in. By the specific activities of this type of project, we intend: to make the working space

adequate, according to the national and European standards; making the existent infrastructure

more useful; supplying modern new research equipment necessary for the objectives of the

project; supplying materials and services necessary for the scientific research. The ACVAPUR

Laboratory develops research activities in the Environmental Protection domain and promotes

PN II, Capacities, M I

93/2007-2009

Director Lecturer PhD NEAGU Anca-Narcisa

ANCS

young researches in their activities. By extending the present infrastructure for research, we aim

to study the eco-morphological and structural characteristic of some new groups of bioindicator

organisms which should form the basis of new biological methods for assessing the lake water

quality.

Being part of the university, this lab permits the young researches the use of highly

technical equipments, and at the same time increasing the degree of the utility of the present

infrastructure. All these may contribute to improving our research capacity for facilitating the

participation in international research projects.

Objectives

Identification of the

main characteristics

of investment

objectives

Preparing the

working space for

laying out new

infrastructure

Equipment, laboratory

adapted furniture and

scientific devices

acquisition, according to

the investment list

Testing of the

capacities of acquired

equipment and

materials

Au

dit

an

d r

esu

lts

rece

pti

on

Part

icip

ati

on

in

th

e in

tern

ati

on

al

rese

arc

h p

roje

cts

Dis

sem

ina

tio

n a

nd

off

icia

l re

cog

nit

ion

of

the

lab

ora

tory

an

d t

hei

r

act

ivit

ies.

Results

Benefits

Exploitation

Beneficiaries

Extending the

research

capacity of the

ACVAPUR

Laboratory

To enlarge the capacity

of an existent laboratory

to permit the study of a

new and important

interdisciplinary field

(Eco-morphology)

To elaborate new

methods for assessing the

water quality based on

specific polluosensitivity

of some water quality

bioindicators, according

to European

requirements.

Development of new

biological methods for

assessing the water

quality

Biological monitoring

water quality

The regional and

national users of

lacustrian

resources (fish

resources,

entertainment,

drinking water

network), local

communities.

Companies

involved in

monitoring of

water quality.

Fundamental

research.

Creating a

research base

compatible with

the European

requirements

and with the

conditions for

international

collaboration

To create new and

modern opportunities of

scientific researches

Participation in national

and international

research projects.

University

research teams

and researchers

involved in the

national network

of the water

quality

monitoring.

Forming new

specialists in the

field.

To improve the young

researcher’s training

and their opportunities

of integrating in

complex national and

international research

team.

Offering subjects for

graduate thesis,

master’s degree and

doctoral theses or

dissertations in this

field.

Students, Master

students, Ph. D.

students, the

Romanian civil

society.

Achieving the

conditions to

have the

national results

recognized in

EU in this field

To adopt EU standards

To adopt

To adopt EU scientific

terminology and

methods

Standardizing biotic

indices similar to those

from other countries

and applicable to the

Romanian aquatic

ecosystems.

Universities,

Research Institutes,

the Romanian

society.

RESEARCH EQUIPMENT

EQUIPMENT Working site Characteristics

1. RESEARCH

BOAT

PN II Capacities, MI,

93/2007-2009

ACVAPUR Laboratory, Potoci

Research Station

L 8,4 m, l 3m, , Yamaha

engine F60 CTL, equipped

with a sampling tool for soft

sediments type Corer,

Kemerer sampler

bathometers, phytoplankton

and zooplankton sampling

nets, Ponar grab, fish nets.

2. Multiparameter

Water Quality Sonde

YSI 6600V2-2,

(Environmental

Monitoring System)


93/2007-2009

ACVAPUR Laboratory, Potoci

Research Station

Assess the water depth,

termperature, dissolved

oxygen, chlorophyll,

conductivity, NO3, NH4,

ORP, turbidity, pH. 30 days

authonomy.

http://en.wikipedia.org/wiki/Sediment

3. EMS 850

Critical Point

Drier


93/2007-2009

Faculty of Biology, Electronic

microscopy laboratory

For scanning electron

microscopy

4. Metalizor EMS

550X Sputter

Coater


93/2007-2009

Faculty of Biology, Electronic

microscopy laboratory

For scanning electron

microscopy

5.

Leica Knife

Maker EM KMR

2.


93/2007-2009

Faculty of Biology, ACVAPUR

Laboratory

Knife maker for

ultramicrotome, used in

electronic transmission

microscopy.

6. LASER

Confocal

Scanning

Microscope) Leica

= DM 5500Q

(direct

microscope) +

Confocal

equipment Leica

TCS SPE +

accesories (Photo

camera).


93/2007-2009


Laboratory

Direct microscope

Fluorescence equipment,

DIC (Diferential Interference

Contrast)

Objectives: - 10x , 20x, 40x,

63x, 100x Lasers: 488, 532

and 635 nm

430-750nm

- 2D: x-y; x-z; x-t

- 3D: x-y-z; x-y-t; x-y-λ

- 4D: x-y-z-t

TIFF, AVI, JPEG

7. Leica Cryostat

CM 1850


93/2007-2009


Laboratory

T -25 - -30 °C.

8.

Leica Rotary

Microtome RM

2265


93/2007-2009


Laboratory

Histological serial sections

9. Leica

Automatic Tissue

Processor TP

1020.


93/2007-2009


Laboratory

Histological tissues

preparation

10. Leica

Autostainer XL

(ST 5010)


93/2007-2009


Laboratory

For staining of histological

slides

11. Embedding

Station Leica EG

1150 (H Heated

Paraffin

Embedding

System+C Cold

Plate – placă de

răcire) PN II Capacities, MI,

93/2007-2009


Laboratory

12. Leica Hote

plate


93/2007-2009


Laboratory

13. Leica EM

AMW –

Automatic

Microwave Tissue

Processor for

electron

Microscopy.

PN II Capacităţi

93/2007-2009


Laboratory

For transmission electron

microscopy.

Books and scientific papers

1. Neagu, A., 2008. Comparative anatomy of the antennal sensilla of Perla marginata,

Dinocras cephalotes and Perlodes microcephalus (Plecoptera) aquatic insect

nymphs, An. Şt. Univ. Al. I. Cuza Iaşi, t LIV: 129-134.

2. Erhan, M., 2008. Diel vertical migration of planktonic crustacean populations in the

Izvoru Muntelui-Bicaz reservoir, An. Şt. Univ. Al. I. Cuza Iaşi, t LIV: 145-151.

3. Dascǎlu, M. M. Miron, I., Neagu, A.–N., 2009. CHIRONOMID LARVAE

COMMUNITIES FROM THE FISH FARM AREA OF IZVORU MUNTELUI –

BICAZ RESERVOIR, An. Şt. Univ. Al. I. Cuza Iaşi, t. LV, in press.

4. Neagu, A., 2009. A NEW TYPE OF ANTENNAL SENSILLUM OF THE

STONEFLY NYMPH, DINOCRAS CEPHALOTES (PLECOPTERA: PERLIDAE),

An. Şt. Univ. Al. I. Cuza Iaşi, t LV, in press.

5. Neagu, A., 2009. GALEAL TIP SENSILLA OF A CARNIVOROUS STONEFLY

NYMPH, PERLA MARGINATA (PLECOPTERA: PERLIDAE), An. Şt. Univ. Al. I.

Cuza Iaşi, t LV, in press.

6. Neagu, A., 2009. DISTAL ANATOMY OF GLOSSA AND PARAGLOSSA OF

STONEFLY NYMPH, PERLODES MICROCEPHALUS (PLECOPTERA:

PERLODIDAE), An. Şt. Univ. Al. I. Cuza Iaşi, t LV, in press.

7. Neagu, A., 2009. SIMILARITIES IN DISTAL ANATOMY OF MAXILLARY AND

LABIAL PALPS OF ZOOPHAGES STONEFLY NYMPHS, An. Şt. Univ. Al. I.

Cuza Iaşi, t LV, in press.

INTERNATIONAL COOPERATION

ECOCATCH – Ecological management of catchments in Europe

2005-2008 European master joint programme

Partners: Uppsala University, Umea University Sweden, UCO Angers,

France, Vrije University Amsterdam Holland, Porto and UCP Porto, Portugal.

Web site: www.ecocatch.eu

ECOCATCH Intensive Programmes: Uppsala University, 2008,

2009, 2010.

Eco-Limnological Summerschool

Partner Konstanz University, Germany: 2006, 2007, 2009, 2010.

http://www.ecocatch.eu/

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