genes, proteins and literature searching ansuman chattopadhyay, phd molecular biology information...
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Genes, Proteins and Literature Searching
Ansuman Chattopadhyay, PhDMolecular Biology Information ServicesHealth Sciences Library SystemUniversity of [email protected]
Molecular Biology Information Service
http://www.hsls.pitt.edu/guides/genetics
HSLS Molecular Biology Information Service
Workshops
Website
Software Licensing
Bioinformatics Consultations
http://www.hsls.pitt.edu/molbio
ED Green et al. Nature 470, 204-213 (2011) doi:10.1038/nature09764
Genomic achievements since the Human Genome Project
Growth of PubMed Citations
Lu et al. Database (Oxford). 2011: baq036
240 K
• Breast Cancer
97K • Schizophrenia
8.7K • BRCA1
61K. p53
Feb 28th 2012
http://www.hsls.pitt.edu/molbio
Research Lifecycle
http://www.hsls.pitt.edu/molbio
Topics
Literature Informatics
Search Engine for Life Sciences
HSLS Mol Bio Information Service
http://www.hsls.pitt.edu/guides/genetics
Literature Informatics
http://www.hsls.pitt.edu/guides/genetics
Literature Informatics
Learn how to
.. find the most appropriate literature
.. mine the literature
.. manage your collected information
.. browse the scientific papers
http://www.hsls.pitt.edu/molbio
Literature Informatics
Comprehensive search: MESH term based PubMed Search PubMed special topics query
Next-generation literature search tools: GoPubMed, eTBlast Quertle HuGE Navigator Utopia Docs
http://www.hsls.pitt.edu/molbio
Literature Informatics
Find published literature with statistical and numerical data on DENGUE OUTBREAKS in India.
What genes are reported to be associated with the disease SCHIZOPHRENIA?
http://www.hsls.pitt.edu/molbio
Searching PubMed
http://www.hsls.pitt.edu/molbio
Literature Informatics
Citations: 20 millionJournals: 5200
Schizophrenia: 96,912Schizophrenia gene: 7382
Dengue outbreaks in India: 329 Dengue outbreaks statistics India: 21
http://www.hsls.pitt.edu/molbio
Challenges
Am I getting everything / the right things?
How to digest this?
http://www.hsls.pitt.edu/guides/genetics
PubMed Search
Am I getting the right things?
Construct PubMed queries with MeSH terms using MeSH browser
http://www.hsls.pitt.edu/guides/genetics
Medical Subject Headings (MeSH)
http://www.hsls.pitt.edu/guides/genetics
The U.S. National Library of Medicine's controlled vocabulary (thesaurus)
Arranged in a hierarchical manner called the MeSH Tree Structures
Updated annually
MeSH Vocabulary Headings
over 24,000 representing concepts found in the biomedical literature (Body Weight, Kidney, Radioactive Waste)
Subheadings attached to headings to describe a specific aspect of a
concept (adverse effects , metabolism, diagnosis, therapy)
Supplementary Concept Records over 172,000 terms in a separate chemical thesaurus -
updated weekly (cordycepin , valspodar , tacrolimus binding protein 4)
Publication Types(Letter, Review, Randomized Controlled Trial)
http://www.hsls.pitt.edu/guides/genetics
MeSH Tree Structure A. Anatomy
B. OrganismsC. DiseasesD. Chemical and DrugsE. Analytical, Diagnostic and
Therapeutic Techniques and EquipmentF. Psychiatry and PsychologyG. Biological SciencesH. Physical SciencesI. Anthropology, Education,
Sociology and Social PhenomenaJ. Technology and Food and BeveragesK. Humanities L. Information Science M. Persons N. Health CareV. Publication Characteristics Z. Geographic Locations
http://www.hsls.pitt.edu/guides/genetics
MeSH Indexing
http://www.hsls.pitt.edu/guides/genetics
Source: NLM
PubMed Search
Retrieve published articles
on Dengue outbreaks in India
including Statistical and numerical data on dengue
outbreaks
http://www.hsls.pitt.edu/guides/genetics
PubMed Query Using MeSH http://www.ncbi.nlm.nih.gov/mesh
http://www.hsls.pitt.edu/molbio
http://www.hsls.pitt.edu/molbio
Find articles on “Dengue outbreaks in India” by searching PubMed using MeSH terms
Link to the video tutorial:http://media.hsls.pitt.edu/media/clres2705/mesh.swf
Resources
• Mesh Browser : http://www.ncbi.nlm.nih.gov/mesh• PubMed: http://www.ncbi.nlm.nih.gov/pubmed
PubMed Query Using MeSH
http://www.hsls.pitt.edu/guides/genetics
PubMed Query Using MeSH
http://www.hsls.pitt.edu/guides/genetics
Building a PubMed Query
http://www.hsls.pitt.edu/guides/genetics
Building a PubMed Query
http://www.hsls.pitt.edu/guides/genetics
Building a PubMed Query
http://www.hsls.pitt.edu/guides/genetics
Building a PubMed Query
http://www.hsls.pitt.edu/guides/genetics
Building a PubMed Query
http://www.hsls.pitt.edu/guides/genetics
Building PubMed QueriesTerm Boolean Term Boolean Term # papers
Dengue AND Outbreaks 823
Dengue * AND Outbreaks 746
Dengue AND Outbreaks AND India 123
Dengue* AND Outbreaks AND India 116
Dengue AND Outbreaks/statistics and numerical data
AND India 7
Dengue* AND Outbreaks/statistics and numerical data
AND India 7
http://www.hsls.pitt.edu/guides/genetics
Useful Links for MeSH
MESH Browser: http://www.ncbi.nlm.nih.gov/mesh Link to Wikipedia, Youtube videos, blogs etc on “medical
subject heading”: http://www.kosmix.com/topic/Medical_Subject_Headings?
18 ways to improve your Pubmed searches by Carrie Iwema http://bitesizebio.com/2008/03/05/18-ways-to-improve-your-pubmed-s
earches/ Searching by using the MeSH Database. NCBI Handbook :
http://www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=helppubmed&part=pubmedhelp#pubmedhelp.Searching_by_using_t
http://www.hsls.pitt.edu/guides/genetics
PubMed Query
What genes are reported to be associated with the disease SCHIZOPHRENIA ?
Topic-Specific PubMed Queries
http://www.hsls.pitt.edu/guides/genetics
Topic-Specific PubMed Querieshttp://www.nlm.nih.gov/bsd/special_queries.html
http://www.hsls.pitt.edu/guides/genetics
http://www.hsls.pitt.edu/molbio
Find genes that are reported to be associated with the disease SCHIZOPHRENIA by searching PubMed
Link to the video tutorial:http://media.hsls.pitt.edu/media/clres2705/scz.swf
Resources
• PubMed Clinical Queries: http://www.ncbi.nlm.nih.gov/pubmed/clinical
Research on Optimal Search Strategies
http://www.hsls.pitt.edu/molbio
PubMed Special Topic Queries
http://www.hsls.pitt.edu/molbio
PubMed Search Filter: Medical Genetics ("schizophrenia"[MeSH Terms] OR
"schizophrenia"[All Fields]) AND (("genetics, medical"[MeSH Terms] OR ("genetics"[All Fields] AND "medical"[All Fields]) OR "medical genetics"[All Fields] OR ("medical"[All Fields] AND "genetics"[All Fields])) OR ("genotype"[MeSH Terms] OR "genotype"[All Fields]) OR "genetics"[Subheading] AND ("genetics"[Subheading] OR "genetics"[All Fields] OR "genetics"[MeSH Terms]))
http://www.hsls.pitt.edu/molbio
PubMed Search Result Display
http://www.hsls.pitt.edu/molbio
How to digestthis?
Data Mining-Knowledge Discovery
http://www.hsls.pitt.edu/molbiohttp://www.hsls.pitt.edu/molbio
PubMed based Tools
http://www.hsls.pitt.edu/molbio
Lu et al. Database (Oxford). 2011; 2011: baq036
http://www.ncbi.nlm.nih.gov/CBBresearch/Lu/search/
Latest Innovations in Literature Searching
GoPubMed Display search results sorted into meaningful topics and subtopics
http://www.hsls.pitt.edu/molbio
GoPubMed
http://www.hsls.pitt.edu/molbio
www.gopubmed.com
http://www.hsls.pitt.edu/molbio
Find genes that are reported to be associated with the disease SCHIZOPHRENIA by using GoPubMed
Link to the video tutorial:http://media.hsls.pitt.edu/media/clres2705/gopubmed.swf
Resources
• GoPubMed: http://www.gopubmed.org/web/gopubmed/2?WEB10O00h00100090000
GoPubMed Search Result Analysis
http://www.hsls.pitt.edu/molbio
GoPubMed Search Result Analysis
http://www.hsls.pitt.edu/molbio
Latest Innovations in Literature Searching
http://www.hsls.pitt.edu/molbio
GoPubMed
Noteworthy links
GoPubMed: exploring PubMed with the Gene Ontology. Doms A,Schroeder M., Nucleic Acids Res. 2005 Jul 1; 33 (Web Server issue):W783-6. http://www.ncbi.nlm.nih.gov/pubmed/15980585
http://www.hsls.pitt.edu/molbio
PubMed driven Web Tools
http://www.ncbi.nlm.nih.gov/CBBresearch/Lu/search/
http://www.hsls.pitt.edu/molbio
PubMed based Tools
http://www.hsls.pitt.edu/molbio
Lu et al. Database (Oxford). 2011; 2011: baq036
http://www.ncbi.nlm.nih.gov/CBBresearch/Lu/search/
Extract gene list from Literature
http://www.hsls.pitt.edu/molbio
http://www.quertle.info/
Questions for Quertle
What genes cause Asthma? What cell lines are used in diabetes research? Which cell types are known to express EGFR? What animals are used in studies for diabetes? Which protein kinases activate TP53?
http://www.hsls.pitt.edu/molbio
http://www.hsls.pitt.edu/molbio
A short video on Quertle
Link to the video tutorial:http://media.hsls.pitt.edu/media/molbiovideos/quertle-ac0212.swf
Resources
• Quertle: www.quertle.info
Search Engine for NIH funded research http://projectreporter.nih.gov/reporter.cfm
http://www.hsls.pitt.edu/molbio
NIH Grant Applications to Gene List
http://www.hsls.pitt.edu/molbio
Search Engine Just for Human GeneticsCDC HuGENavigator : http://hugenavigator.net/
http://www.hsls.pitt.edu/molbio
http://www.hsls.pitt.edu/molbio
Find human genes reported to be associated with Asthma
Find human SNPs reported to be associated with Asthma
Link to the video tutorial:http://media.hsls.pitt.edu/media/clres2705/asthma.swf
Resources
• HugeNavigator:
http://hugenavigator.net/HuGENavigator/home.do
GWAS Catalog http://www.ebi.ac.uk/fgpt/gwas/#timeseriestab
http://www.hsls.pitt.edu/molbio
Search Engine Just for Human Genetics
http://www.hsls.pitt.edu/molbio
Search Engine Just for Human GeneticsCDC HuGENavigator : http://hugenavigator.net/
http://www.hsls.pitt.edu/molbio
Search Engine Just for Human Geneticshttp://hugenavigator.net/HuGENavigator/huGEPedia.do
http://www.hsls.pitt.edu/molbio
Search Engine Just for Human GeneticsCDC HuGENavigator : http://hugenavigator.net/
http://www.hsls.pitt.edu/molbio
Find Disease Causing SNPs
What SNPs are associated with “Schizophrenia”?
http://hugenavigator.net/HuGENavigator/gWAHitStartPage.do
http://www.hsls.pitt.edu/molbio
Hands On
Search PubMed and retrieve a list of genes that can serve as biomarkers for Alzheimer Disease
Text-based Similarity Search Tools
Search Box:
http://www.hsls.pitt.edu/molbio
Text Similarity Search Tools
eTBLASThttp://etest.vbi.vt.edu/etblast3/
http://www.hsls.pitt.edu/molbio
Text Similarity Search Tools
eTBLAST
http://www.hsls.pitt.edu/molbio
Text Similarity Search Tools
http://www.hsls.pitt.edu/molbio
Déjà Vu: a Database of Highly Similar Citationshttp://dejavu.vbi.vt.edu/dejavu/duplicate/
http://www.hsls.pitt.edu/molbio
Automated email Notification Tool
http://www.ncbi.nlm.nih.gov/sites/myncbi/
Save your searches at My NCBI and set up an email notification on new publication based on your search query
http://www.hsls.pitt.edu/molbio
Reference Management Tools
Connotea
CiteULike
Mendaley
Zotero
online
downloadable
Scientific Research Papers
Web pages
EndNoteRefworks
http://www.hsls.pitt.edu/molbio
PDF Reader
http://www.hsls.pitt.edu/molbio
NextGen PDF Reader: Utopia docs
http://www.hsls.pitt.edu/molbio
Search Engine for Life Scientists
http://www.hsls.pitt.edu/molbio
Molecular Databases
Nucleic Acids Research : Annual databases Issue NAR: Annual Web Server Issue Oxford Journal : Bioinformatics BioMedCentral: BMC Bioinformatics
http://www.hsls.pitt.edu/molbio
Growth of bioinformatics tools
Biomedical & Life Sciences Search Engines
OBRC : University of Pittsburgh
http://www.hsls.pitt.edu/molbio/obrc
Bioinformatics.cahttp://bioinformatics.ca/links_directory/
OReFil : University of Tokyo
http://orefil.dbcls.jp/
http://www.hsls.pitt.edu/molbio
Peptide Sequence
>nxp|NX_P00533-1|EGFR|Epidermal growth factor receptor|Iso 1 MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEV VLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALA VLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDF QNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGC TGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYV VTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFK NCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAF ENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKL FGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCN LLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVM GENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVV ALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGS GAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGI CLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAA RNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSY GVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPK FRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQ QGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTED SIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLN TVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRV
APQSSEFIGA
http://www.hsls.pitt.edu/molbio
Search for bioinformatics ResourcesLocate online tools that predict phosphorylation sites in a protein sequence.
Link to the video tutorial:http://media.hsls.pitt.edu/media/Search%20Bioinfo%20Resoursec.swf
Resources
• Search.HSLS.MolBio :
Life Sciences Search Enginehttp://www.hsls.pitt.edu/molbio/
http://www.hsls.pitt.edu/molbio
Hands on
(a) Locate two online databases on microRNA targets, and report their URLs.
(b) Identify one database that store all HIV inhibiting siRNA data and cite its URL.
(c) Cite a paper offering a step-by-step guide
for analyzing ChIP-seq data.
Summary Construct a comprehensive PubMed query:
MESH Browser Retrieve gene/protein/disease information straight from a
PubMed search : GoPubmed, Find suitable journals for manuscript submission, etc… eTBLAST
Find disease causing genes and disease causing SNPs: HUGENavigator_ Geneprospector, Phenopedia,
GWASIntegrator
http://www.hsls.pitt.edu/guides/genetics
Summary
Setup email alert for new publications My NCBI
Store articles online CiteULike
Analyze popularity of a published article Post Publication Metrics
Search for databases and software OBRC
http://www.hsls.pitt.edu/guides/genetics
Links MESH Database: http://www.ncbi.nlm.nih.gov/mesh PubMed Special Topic Queries:
http://www.nlm.nih.gov/bsd/special_queries.html GoPubMed: http://www.gopubmed.com CDC HuGENavigator : http://hugenavigator.net/ Novoseek: http://www.novoseek.com etBLAST: http://etest.vbi.vt.edu/etblast3/ déjà vu: http://dejavu.vbi.vt.edu/dejavu/duplicate/ MyNCBI: http://www.ncbi.nlm.nih.gov/sites/myncbi/ CiteUlike: http://www.citeulike.org/ Publish or Perish: http://www.harzing.com/pop.htm OBRC: http://www.hsls.pitt.edu/guides/genetics/obrc
http://www.hsls.pitt.edu/guides/genetics