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Genetic Engineering

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Page 1: Genetic Engineering. What Is This? Do you hear what I hear?

Genetic Engineering

Page 2: Genetic Engineering. What Is This? Do you hear what I hear?

What Is This?

Page 3: Genetic Engineering. What Is This? Do you hear what I hear?

• Do you hear what I hear?

Page 4: Genetic Engineering. What Is This? Do you hear what I hear?

Selective Breeding• Allowing only those animals with

desired characteristics to produce the next generation

• Humans use selective breeding to pass desired traits on to the next generation of organisms

• Inbreeding- develop pure lines

• Hybrid- usually bigger and stronger

Page 5: Genetic Engineering. What Is This? Do you hear what I hear?

Examples of Selective Breeding

Page 6: Genetic Engineering. What Is This? Do you hear what I hear?

Genetic Engineering

• Transgenic organisms contain recombinant DNA (foreign DNA)

• 3 Step Process

–1. Isolate - cleave via restriction enzymes

–2. Attach – “sticky ends”

–3. Transfer – vector, usually bacterial DNA

Page 7: Genetic Engineering. What Is This? Do you hear what I hear?

Tobacco with “Firefly DNA”

Page 8: Genetic Engineering. What Is This? Do you hear what I hear?

Gel Electrophoresis

Page 9: Genetic Engineering. What Is This? Do you hear what I hear?

DNA Sequencing

Page 10: Genetic Engineering. What Is This? Do you hear what I hear?

• ..\..\..\genetransfer30.mov

Page 11: Genetic Engineering. What Is This? Do you hear what I hear?

Applications of DNA Technology or Biotechnology

• Cloning• Recombinant DNA in technology,

medicine, and agriculture• Transgenic organisms• Gene therapy

–Sheep alpha-1 antitrypsin for treatment of emphysema

–Goats CFTR protein for treatment of Cystic Fibrosis

Page 12: Genetic Engineering. What Is This? Do you hear what I hear?

Transgenic Animals• Transgenic livestock have been

produced with extra copies of growth hormone genes

• Such animals grow faster and produce meat that is less fatty than that from ordinary animals

• Efforts are now underway to produce transgenic chickens that will be resistant to the bacterial infections that sometimes cause food poisoning

Page 13: Genetic Engineering. What Is This? Do you hear what I hear?

Transgenic Plants

• (2000) 52 % of the soybeans and 25 % of the corn grown in the United States were transgenic

• Many contain genes that produce a natural insecticide, so the crops do not have to be sprayed with synthetic pesticides

• Others have genes that enable them to resist weed-killing chemicals

Page 14: Genetic Engineering. What Is This? Do you hear what I hear?

Cloned Animals

Page 15: Genetic Engineering. What Is This? Do you hear what I hear?

Gene Therapy

Page 16: Genetic Engineering. What Is This? Do you hear what I hear?

• ..\Biologix_Manipulating DNA.asx


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