genome editing / crispr-cas9 - eppendorf › uploads › media › workflow_card_geno… · the...

2
Prokaryotic and eukaryotic cells The recent CRISPR-Cas9 genome editing method uses RNA-guided nuclease. That makes the process faster, easier and more specific compared to other gene editing methods. Since the DNA-binding element is RNA it is simple and cheap to manipulate and it allows sequence specific programming. The high targeting efficiency, re- sults in significant time saving, e.g., for the generation of knockout mice. Furthermore, alteration of multiple genes in one step is possible (multiplexing). In basic research CRISPR is mainly used in cell line engi- neering and animal genetic engineering (e.g. of disease models) to investigate gene function. In applied biotech- nology genome editing is employed for the improvement of agricultural animals and plants, as well as the produc- tion of biopharmaceuticals or other biological molecules in the industry. Genome editing / CRISPR-Cas9

Upload: others

Post on 04-Jul-2020

7 views

Category:

Documents


0 download

TRANSCRIPT

Page 1: Genome editing / CRISPR-Cas9 - Eppendorf › uploads › media › Workflow_Card_Geno… · The recent CRISPR-Cas9 genome editing method uses RNA-guided nuclease. That makes the process

Prokaryotic and eukaryotic cellsThe recent CRISPR-Cas9 genome editing method uses RNA-guided nuclease. That makes the process faster, easier and more specific compared to other gene editing methods. Since the DNA-binding element is RNA it is simple and cheap to manipulate and it allows sequence specific programming. The high targeting efficiency, re-sults in significant time saving, e.g., for the generation of knockout mice. Furthermore, alteration of multiple genes in one step is possible (multiplexing).

In basic research CRISPR is mainly used in cell line engi-neering and animal genetic engineering (e.g. of disease models) to investigate gene function. In applied biotech-nology genome editing is employed for the improvement of agricultural animals and plants, as well as the produc-tion of biopharmaceuticals or other biological molecules in the industry.

Genome editing / CRISPR-Cas9

Page 2: Genome editing / CRISPR-Cas9 - Eppendorf › uploads › media › Workflow_Card_Geno… · The recent CRISPR-Cas9 genome editing method uses RNA-guided nuclease. That makes the process

www.eppendorf.com/workflows

Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany. All rights reserved, including graphics and images. Copyright © 2016 by Eppendorf AG. Order No.: AQ41 311 020/GB1/3T/0716/NB/STEFF · Carbon neutrally printed in Germany.

Your local distributor: www.eppendorf.com/contactEppendorf AG · 22331 Hamburg · [email protected] · www.eppendorf.comEppendorf North America, Inc. · Phone: 800-645-3050 [email protected] · www.eppendorfna.com Eppendorf Canada Ltd. · Phone: 800-263-8715 [email protected] · www.eppendorf.ca

Always Worth a Look

Cell Culture Consumables Photometers Shakers

Analysis and detection

Harvesting and preparation

Transfection and cultivation (eukaryotic cells)

Preparation and purification

Vector generation

Transformation and cultivation (prokaryotic cells)

Genome editing / CRISPR-Cas9(Prokaryotic and eukaryotic cells)

Centrifuges

Photometers

Thermo mixing devices

Thermocyclers

Conical tubes

Cuvettes

Tubes and plates

LoBind consumables

CO2 Incubators Conical tubesDishes, flasks and plates MicroinjectorsMicromanipulators

Thermocyclers Photometers Accessories (e.g. Kit rotor)Thermo mixing devices Concentrator

Electroporator Shaker Pipettes and tipsCentrifuges Photometers

Thermo mixing devices Thermocyclers PCR consumables Tubes Pipettes and tips

Biopur® consumables

PCR consumables