Genomics and ProteomicsDNA sequencing: ddNTP chain termination

Sequencing entire genomes: BAC to BAC

Sequencing entire genomes: Shotgun Method

Genomics based approach in Genomics based approach in ArabidopsisArabidopsis

*Arabidopsis genome sequenced in 2000

Rat r-protein sequences

BLAST algorithm search used to find Arabidopsis ribosomal protein

genes

Barakat, A. et al. (2001) Plant Physiology vol. 127, pp. 398-415

249 Total 249 Total genesgenes

80 Total 80 Total putative r-putative r-proteinsproteins

rpl7-2 rpl7-2

rps17-1, rps17-2rps17-1, rps17-2

rps16-1 rps16-1

rps25-1 rps25-1

rps4-1, rps4-1, rpl8-1rpl8-1

rpl14-1rpl14-1

rps30-1rps30-1, , rps15a-2rps15a-2

rpl28-1rpl28-1, rpl31-1, rpl31-1

rps25-2*rps25-2*

rpl4-3*rpl4-3*

rpl39-1rpl39-1

rpp2-1, rpp2-2rpp2-1, rpp2-2

rpl10a-2rpl10a-2

rps12-3rps12-3

rps3-1, rpl27-1rps3-1, rpl27-1

rpl23-2rpl23-2

rpl24-1rpl24-1

rps14-1, rpl40-1rps14-1, rpl40-1

rpl18a-2, rpl37a-1*rpl18a-2, rpl37a-1*

rps5-1, rpl36-1rps5-1, rpl36-1

rpl23a-1, rpl35-2rpl23a-1, rpl35-2

rps15a-3rps15a-3

rps26-1, rps26-2rps26-1, rps26-2

rpp0-1, rpl41-3rpp0-1, rpl41-3

rps2-3, rpl11-1, rpl38-1rps2-3, rpl11-1, rpl38-1

rpl7-3rpl7-3

rps27-1rps27-1

rps27a-2rps27a-2

rpl7a-1, rpl18-1rpl7a-1, rpl18-1

rpl12-1rpl12-1

rpl35-4, rpl36-3 rpl35-4, rpl36-3 rps23-2, rpl4-4 rps23-2, rpl4-4 rps28-2 rps28-2

rps17-4 rps17-4 rps4-2 rps4-2

rps15-2, rps15-3, rps15-4rps15-2, rps15-3, rps15-4

rps6-2rps6-2

rps9-2rps9-2rps7-3, rps16-3, rps19-2rps7-3, rps16-3, rps19-2

rps8-1, rpl10a-3rps8-1, rpl10a-3rps11-3, rpl13-4rps11-3, rpl13-4

rps21-3, rpl22-3rps21-3, rpl22-3

rps24-2, rpl18-3rps24-2, rpl18-3

rps3-3rps3-3

rps4-3*rps4-3*

rpp2-5, rpl5-2rpp2-5, rpl5-2

rps9-3, rpl5-3*rps9-3, rpl5-3*

rps10-2rps10-2

rpl3-3*rpl3-3*

rps15-5rps15-5

rpl32-2rpl32-2

rpl11-4rpl11-4

rps27-4, rpp1-3rps27-4, rpp1-3

rpl13a-4rpl13a-4

rps10-3rps10-3

rps4-4, rps8-2, rps4-4, rps8-2, rpp3-2rpp3-2

rps30-3, rpl31-3rps30-3, rpl31-3

rps15a-6rps15a-6, rpl12-3, rpl12-3

rps19-3, rps19-3, rps20-3rps20-3

rps15-6*rps15-6*

rps28-3rps28-3

rpl26-2rpl26-2

rps13-2 rps13-2

rpp1-2 rpp1-2

rpl19-3 rpl19-3

rpl9-4rpl9-4

rps18-3rps18-3

rps9-1*rps9-1*

rpl13a-3rpl13a-3

rpl27-3rpl27-3

rpl36a-2rpl36a-2

rpl15-1rpl15-1

rpl15-2rpl15-2

rpl32-1rpl32-1

rpl11-3rpl11-3

rpl14-2, rpl31-2rpl14-2, rpl31-2

rps10-1rps10-1, , rpp3-1rpp3-1

rps30-2rps30-2, , rpl28-3rpl28-3

rps11-2, rps11-2, rps15a-5rps15a-5

rps6-1, rpl39-3rps6-1, rpl39-3

rps3a-2, rps3a-2, rps25-4*rps25-4*

rps29-3, rps29-4*rps29-3, rps29-4*

rpl8-3rpl8-3

rps25-5rps25-5

rpl13a-1,rpl13a-1, rpl29-1, rpl29-2rpl29-1, rpl29-2

rps21-2, rpl36-2rps21-2, rpl36-2rpl23a-2, rps2-4 rpl23a-2, rps2-4 rpl11-2, rpl21-6*rpl11-2, rpl21-6*rps26-3, rpl35a-4, rpl41-7rps26-3, rpl35a-4, rpl41-7rps27a-3rps27a-3rpl38-2, rpl37a-3, rps13-1rpl38-2, rpl37a-3, rps13-1rps27-2, rps27-3*rps27-2, rps27-3*rpl17-2rpl17-2

rps7-2, rps19-1, rpl39-2rps7-2, rps19-1, rpl39-2rps3a-1, rps24-1rps3a-1, rps24-1rpsa-2, rps16-2, rpl23-3rpsa-2, rps16-2, rpl23-3rpl41-4rpl41-4rpl18-2, rpl22-2rpl18-2, rpl22-2rpl37a-2, rpl41-5rpl37a-2, rpl41-5rps14-2, rps17-3rps14-2, rps17-3rps5-2, rpp0-2rps5-2, rpp0-2rpp0-3rpp0-3rps28-1rps28-1rpl7-4, rpl18a-3 rpl7-4, rpl18a-3

rpl19-2, rpl19-2, rpl37-3rpl37-3rpl30-3rpl30-3rpl27-2rpl27-2rpl36a-1rpl36a-1rpl5-1, rpl13a-2rpl5-1, rpl13a-2rps21-1*rps21-1*rpp2-3, rpl34-3rpp2-3, rpl34-3rps25-3*rps25-3*rps20-1rps20-1rps29-1, rps29-2rps29-1, rps29-2rps15a-4rps15a-4, rpp2-4, rpp2-4rps20-2rps20-2rpl13-1*rpl13-1*rpl13-2, rpl13-3rpl13-2, rpl13-3rps11-1rps11-1rpl8-2, rpl26-1rpl8-2, rpl26-1rps14-3, rpl40-2rps14-3, rpl40-2rps3-3, rpl12-2rps3-3, rpl12-2rpl24-2rpl24-2rpl35-3rpl35-3

rpl28-2*, rpl28-2*, rpl41-6rpl41-6

rpl10a-1rpl10a-1

rpp1-1,rpp1-1, rpl23-1, rps15-1rpl23-1, rps15-1

rpl19-1, rpl22-1rpl19-1, rpl22-1

rps15a-1, rpl35a-1rps15a-1, rpl35a-1

rpl21-1, rpl21-2*,rpl21-1, rpl21-2*, rpl21-3rpl21-3

rpl10-1rpl10-1

rpl27a-1, rpl14-1rpl27a-1, rpl14-1

rpl35-1, rps23-1rpl35-1, rps23-1

rpl37-1, rps12-1rpl37-1, rps12-1

rpl6-1rpl6-1

rpl27a-2, rps18-1, rpl27a-2, rps18-1, rps27a-1rps27a-1

rpl10-2, rpl10-2, rpl34-1rpl34-1

rpl18a-1rpl18a-1

rpl21-4*rpl21-4*

rpl17-1rpl17-1

rpl19-1,rpl19-1, rpl19-2, rpl19-3rpl19-2, rpl19-3

rps18-2rps18-2

rpl4-2*rpl4-2*

rpl35a-2, rpl30-1rpl35a-2, rpl30-1

rpl3-1rpl3-1

rps7-1rps7-1

rpl37-2rpl37-2

rpl41-1rpl41-1, rpl21-5 , rpl21-5

rps2-1rps2-1

rpl3-2, rps2-2 rpl3-2, rps2-2

rpl10-3, rpl17-2rpl10-3, rpl17-2

rpl27a-3rpl27a-3, , rpl34-2rpl34-2

rpsa-1rpsa-1

rpl6-2rpl6-2, rpl6-3 , rpl6-3

rpl35a-3 rpl35a-3

rpl30-2 rpl30-2

rpl41-2* rpl41-2*

rps12-2* rps12-2*

rpl7-1 rpl7-1

00

1010

2020

3030

4040

5050

6060

7070

8080

9090

100100

110110

120120

130130

140140

cMcM 1 2 33 4 5

538 kb/ 538 kb/ r-proteinr-protein

436 kb/ 436 kb/ r-proteinr-protein

331 kb/ 331 kb/ r-proteinr-protein

605 kb/ 605 kb/ r-proteinr-protein

519 kb/ 519 kb/ r-proteinr-protein

Genomics and Proteomics

DNA Microarrays and Microchips

Applications of Genomics: Functional Genomics

DNA Microarrays and Microchips

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Transcriptional Response of Human Fibroblasts to Serum

Applications of Genomics: Functional Genomics

Whole Chromosome Transcriptional Mapping

Applications of Genomics: Functional Genomics

DNA-Protein Interactions

Applications of Genomics: Functional Genomics

SNPs and Pharmacogenomics

ProteomicsMADLI-TOF Mass Spectrometry

Proteomics Approach in Arabidopsis

Chang, et al., (2005) Plant Physiology. 137: 848-862

ProteomicsProtein Microchip

ProteomicsProtein Interactions