genomics application using gramene

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Computational Genomics Genomics: Application using Genomics: Application using GRAMENE S.Prasanth Kumar, S.Prasanth Kumar, Bioinformatician Bioinformatician S.Prasanth Kumar Dept. of Bioinformatics Applied Botany Centre (ABC) Gujarat University, Ahmedabad, INDIA www.facebook.com/Prasanth Sivakumar FOLLOW ME ON ACCESS MY RESOURCES IN SLIDESHARE prasanthperceptron CONTACT ME prasanthbioinformatics@gmail. com (Many Slides are from GRAMENE Tutorial Section)

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GRAMENE application genome browser and the different utilities

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Page 1: Genomics application using gramene

S.Prasanth Kumar, S.Prasanth Kumar, BioinformaticianBioinformatician

Computational Genomics

Genomics: Application using Genomics: Application using GRAMENE

S.Prasanth Kumar, S.Prasanth Kumar, BioinformaticianBioinformatician

S.Prasanth Kumar Dept. of Bioinformatics Applied Botany Centre (ABC) Gujarat University, Ahmedabad, INDIA

www.facebook.com/Prasanth Sivakumar

FOLLOW ME ON

ACCESS MY RESOURCES IN SLIDESHARE

prasanthperceptron

CONTACT ME

[email protected]

(Many Slides are from GRAMENE Tutorial Section)

Page 2: Genomics application using gramene

GRAMENE Home page

Page 3: Genomics application using gramene

Browsing

Type in the search term (a clone/feature’s accession

number or name) and press search icon.

2: or Choose species from available genomes (see Slide

7 for Rice).

Page 4: Genomics application using gramene

Search Results

Click to view in browser

Page 5: Genomics application using gramene

Genome Browsing

Or choose a Synteny map to

view

Or you may select a chromosome to browse

by clicking on it

ExamplesAnnotation Info

Information on the assembly

Click to change species

Page 6: Genomics application using gramene

Synteny Rice Chr. & Rice

Clicking on a link on the map will let Open a menu of options.

Identifies the rice chromosome you are browsing, which is in the center of the diagram.

Click on a maize chromosome and select “center gene list” to show rice synteny to that maize chromosome.

Navigation for choosing an alternate chromosome or syntenic species

Page 7: Genomics application using gramene

Rice Chromosome

Chromosome information

Click on image to zoom in

Click go to view synteny with current chromosome

MapView shows distribution of features at

chromosome scales.

Use Jump to ContigView at bottom of page to select a region by two features.

You are here

Choose another chromosome to view.

Page 8: Genomics application using gramene

Contig View

Use Jump to Contiview, to select a region by two features.

Page 9: Genomics application using gramene

Viewing Results - Contig View

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Chromosome View

Identifies which chromosome you are

viewing. Clicking on the chromosome will move

view to that area

Area in red outlined box on Chromosome is the

area you are viewing and is enlarged to fill the

overview below.

Entire Chromosome is displayed at the top

Genomes Menu options

Page 11: Genomics application using gramene

Overview

Page 12: Genomics application using gramene

Gene Order (Synteny)Detailed Overview

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Base Pair View

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Genomic Sequence

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Gene Splice Site Image

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Gene Tree

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Exons Information

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Protein Information

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Retrieving Sequence

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Export Sequence as Fasta

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EMBL flat file output

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Thank You For Your Attention !!!