genotyping transgenic mice
DESCRIPTION
Genotyping Transgenic Mice. By: Natalie Saldana & Dr. Taha Merghoub. Introduction. Human Disease. Melanoma - serious form of skin cancer. Mouse Model – used to mimic the human disease. Transgenic Mouse. Hypothesis. - PowerPoint PPT PresentationTRANSCRIPT
Genotyping Transgenic MiceBy: Natalie Saldana
&
Dr. Taha Merghoub
Introduction
Mouse Model – used to mimic the human disease
Melanoma - serious form of skin cancer
Transgenic Mouse
Human Disease
Hypothesis
The introduction of Human melanoma mutation in mice will induce this disease in mice
Why Mice?
They are genetically similar to human beingsAffordable and easy to maintainAble to reproduce within 3 weeksShort life spanSmall
Transgenic Mouse
Genotyping
The process of testing individuals in order to reveal any specific gene that has been inherited from that individual
Includes other methods like PCR, DNA sequencing, and Southern blot
The goal is to understand the functions and roles of genes and to test new therapies in melanoma
Materials PCR
Taq Polymerase
Buffer
MgCl2
Primers
dNTPs
DNA
H2O
Gel Electrophoresis
Agarose
TAE Buffer
Ethidium Bromide
Trays & Combs
Gel Box
Power Supply
UV Light
1 Kb Ladder
Loading Dye
DNA Extraction
Mouse Tails
Qiagen DNeasy Protocol
Thermal Cycler (PCR Machine)
PCR Procedure
Observations & Results
Ink4A WT
Ink4A KO
We were successful in genotyping the mice which we will use for future experiments.
Conclusion and Future Directions
We have identified Heterozygous and KO Ink4a mice.
The Ink4a mice will be crossed with NRas transgenic mice in order to obtain a mouse with melanoma.
Reference
http://users.ugent.be/~avierstr/principles/pcr.html http://www.accessexcellence.org/RC/VL/GG/
transgenic.html http://users.rcn.com/jkimball.ma.ultranet/
BiologyPages/T/TransgenicAnimals.html http://sunsite.berkeley.edu/PCR/whatisPCR.html http://www.agrf.org.au/Default.aspx?tabid=85 http://ghr.nlm.nih.gov/ghr/glossary/genotyping http://www.identigene.com/SWIMX/Products/
mouse_genotyping.asp http://en.wikipedia.org/wiki/Mouse http://en.wikipedia.org/wiki/Genotyping http://www.skincancer.org/melanoma/index.php
Acknowledgements
Dr. Taha Merghoub Diana Hong Houghton Laboratory Dr. Sat Bhattacharya Harlem Children Society