gh sensitivity dna assay de72901460 2010-11-06 16-50-18 ...banana_slug... · description number...

Instrument Name: DE72901460 Firmware:Serial#:Assay Information:

C.01.069DE72901460

Instrument Information:

Assay Origin Path: C:\Program Files\Agilent\2100 bioanalyzer\2100expert\assays\dsDNA\High Sensitivity DNA.xsy

Assay Class:Version:Assay Comments:

High Sensitivity DNA Assay1.0Copyright © 2003-2009 Agilent Technologies

Chip Information:

Chip Comments:

Type: G2939A

Chip Lot #:Reagent Kit Lot #:

CU/COVARIS 1 sample 2 sample 3

sample 4 sample 5 sample 6

sample 7 sample 8 LB PRINCT

WT PRINCT CHIP PRINCT

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 3/27/2011 9:22:36 PM

2100 expert_High Sensitivity DNA Assay_DE72901460_2010-11-06_16-50-18.xad Page of1 8

Created:Modified:

11/6/2010 4:50:19 PM3/27/2011 9:21:50 PMData Path:

High Sensitivity DNA AssayC:\...gh Sensitivity DNA Assay_DE72901460_2010-11-06_16-50-18.xad

Assay Class:

Electrophoresis File Run Summary

Sample Name Sample Comment Rest. Digest Status Observation Result Label Result ColorCU/COVARIS 1sample 2sample 3sample 4sample 5sample 6sample 7sample 8LB PRINCTWT PRINCTCHIP PRINCTLadder

Chip Lot # Reagent Kit Lot #

Chip Comments :



Created:Modified:

11/6/2010 4:50:19 PM3/27/2011 9:21:50 PMData Path:


Assay Class:

Electrophoresis File Run Summary (Chip Summary)

General Analysis SettingsNumber of Available Sample and Ladder Wells (Max.) : 12Minimum Visible Range [s] : 32Maximum Visible Range [s] : 138Start Analysis Time Range [s] : 33End Analysis Time Range [s] : 137.5Ladder Concentration [pg/µl] : 1950Uses Standard Area for Ladder FragmentsLower Marker Concentration [pg/µl] : 125Upper Marker Concentration [pg/µl] : 75Used Upper Marker for QuantitationStandard Curve Fit is Point to PointShow Data Aligned to Lower and Upper Marker

Integrator SettingsIntegration Start Time [s] : 33.05Integration End Time [s] : 137Slope Threshold : 0.8Height Threshold [FU] : 5Area Threshold : 0.1Width Threshold [s] : 0.6Baseline Plateau [s] : 0.5

Filter SettingsFilter Width [s] : 0.5Polynomial Order : 4

Ladder Ladder Peak Size Area1 35 1602 50 2103 100 2084 150 2215 200 2426 300 2707 400 3058 500 3069 600 33610 700 32111 1000 36612 2000 41313 3000 41114 7000 40015 10380 214



Created:Modified:

11/6/2010 4:50:19 PM3/27/2011 9:21:50 PMData Path:


Assay Class:

Electrophoresis Assay Details

Peak table for sample 7 : sample 7Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 382 235.19 933.63 395 84.23 322.74 401 65.86 248.75 420 64.57 233.16 430 90.45 318.47 450 164.46 553.98 10,380 75.00 10.9 Upper MarkerRegion table for sample 7 : sample 7From[bp]

To [bp] Corr.Area

% ofTotal

Average Size[bp]

Size distribution inCV [%]

Conc.[pg/µl]

Molarity[pmol/l]

Color

322 519 322.7 92 411 10.5 738.91 2,759.4

sample 7

Overall Results for sample 7 : sample 7Number of peaks found: 6 Noise: 0.1

Corr. Area 1: 322.7



Created:Modified:

11/6/2010 4:50:19 PM3/27/2011 9:21:50 PMData Path:


Assay Class:

Electropherogram Summary

Peak table for sample 8 : sample 8Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 10,380 75.00 10.9 Upper MarkerRegion table for sample 8 : sample 8From[bp]

To [bp] Corr.Area

% ofTotal

Average Size[bp]

Size distribution inCV [%]

Conc.[pg/µl]

Molarity[pmol/l]

Color

294 470 22.3 79 372 10.9 59.58 245.4

sample 8

Overall Results for sample 8 : sample 8Number of peaks found: 0 Noise: 0.1

Corr. Area 1: 22.3



Created:Modified:

11/6/2010 4:50:19 PM3/27/2011 9:21:50 PMData Path:


Assay Class:

Electropherogram Summary Continued ...



Created:Modified:

11/6/2010 4:50:19 PM3/27/2011 9:21:50 PMData Path:


Assay Class:

Gel Image

Standard Curve



Created:Modified:

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Assay Class:

Curves

Description Number Source Category Sub Category Time Time Zone User HostRun ended onport 6 (Numberof wellsacquired: 12)

Instrument Run 11/6/20105:31:43 PM

(GMT --07:00)Pacific StandardTime

Alex OMNIBOOK

Run started onport 6 (File:C:\ProgramFiles\Agilent\2100bioanalyzer\2100expert\Data\2010-11-06\2100expert_HighSensitivity DNAAssay_DE72901460_2010-11-06_16-50-18.xad)



Alex OMNIBOOK

Product Number: G2939A



Alex OMNIBOOK

Name : Instrument Run 11/6/20104:50:26 PM


Alex OMNIBOOK

Vendor : AgilentTechnologies



Alex OMNIBOOK

Serial# :DE72901460



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Firmware :C.01.069



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Cartridge :Electrode



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Created:Modified:

11/6/2010 4:50:19 PM3/27/2011 9:21:50 PMData Path:


Assay Class:

Run Logbook

gh sensitivity dna assay de72901460 2010-11-06 16-50-18 ...banana_slug... · description number...

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