gingiva - dental.subharti.orgdental.subharti.org/periodonotics/gingiva_microscopy.pdf- present in...
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GINGIVA MICROSCOPIC FEATURES
Dr. Mayur Kaushik
1 Dr. Mayur Kaushik, Subharti Dental College, SVSU
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GINGIVA
EPITHELIUM
- Stratified Squamous Epithelium
CONNECTIVE TISSUE
- Papillary & Reticular Layers
2 Dr. Mayur Kaushik, Subharti Dental College, SVSU
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Dr. Mayur Kaushik, Subharti Dental College, SVSU
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LAYERS OF EPITHELIUM
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Dr. Mayur Kaushik, Subharti Dental College, SVSU
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PROCESSES MAINTAINING
INTEGRITY
PROLIFERATION
- Division of cells.
- Occurs in basal cell layer.
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DIFFERENTIATION ( KERATINIZATION)
Increased number of tonofilaments &
intercellular junctions.
Production of keratohyaline granules.
Disappearance of nucleus.
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Dr. Mayur Kaushik, Subharti Dental College, SVSU
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KERATINIZATION
ORTHOKERATINIZED
- No nuclei in stratum corneum.
- Well defined stratum granulosum.
PARAKERATINIZED
- Pyknotic nuclei in stratum corneum.
- Keratohyaline granules dispersed. 8
Dr. Mayur Kaushik, Subharti Dental College, SVSU
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KERATIN PROTEINS
Numbered in sequence contrary to their molecular weight.
K1 (68 Kd) : Main component of stratum corneum.
K 19 (40 Kd)
OTHER PROTEINS :
- Keratolinin
- Involucrin
- Filaggrin 9
Dr. Mayur Kaushik, Subharti Dental College, SVSU
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CELLS OF EPITHELIUM
KERATINOCYTES
- Principal cells of epithelium
NON-KERATINOCYTES (Clear Cells)
- Langerhan’s Cells
- Merkel Cells
- Melanocytes
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Dr. Mayur Kaushik, Subharti Dental College, SVSU
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CELL JUNCTIONS
DESMOSOMES : Connection between
keratinocytes revealed at electron
microscopic level.
Consist of 2 dense attachment plaques
with an intermediate gap.
ZONA OCCLUDENS (tight junctions):
Membranes of adjacent cells appear to be
fused.
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TONOFILAMENTS The morphologic expression of the
cytoskeleton of keratin proteins.
Radiate in a brushlike fashion from the
attachment plaques into the cytoplasm of
the cells.
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KERATOHYALINE GRANULES
Present in stratum granulosum
KERATINOSOMES /
ODLAND BODIES
Present in uppermost cells of stratum
spinosum.
Modified lysosomes which contain acid
phosphatase.
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Dr. Mayur Kaushik, Subharti Dental College, SVSU
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NON - KERATINOCYTES
MELANOCYTES
- Present in basal & spinous layers.
- Synthesize melanin in organelles
called melanosomes.
TYROSINE
DOPA
MELANIN 14
Dr. Mayur Kaushik, Subharti Dental College, SVSU
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LANGERHAN’S CELLS
- Modified monocytes derived from
bone marrow.
- Function: Immune reaction – antigen
presenting cells to lymphocytes.
MERKEL’S CELLS
- Harbour nerve endings.
- Tactile perceptors.
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BASAL LAMINA
Joins epithelium with underlying
connective tissue.
Thickness : 300-400 A.
PARTS:
1) Lamina lucida
2) Lamina densa
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Dr. Mayur Kaushik, Subharti Dental College, SVSU
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LAMINA LUCIDA – Towards epithelium &
mainly composed of the glycoprotein –
laminin.
LAMINA DENSA – Towards connective
tissue & contains Type IV collagen.
Function : Permeable to fluids but acts as
a barrier to particulate matter.
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Dr. Mayur Kaushik, Subharti Dental College, SVSU
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GLYCOGEN
Concentration in normal gingiva is
inversely related to degree of
keratinization & amount of inflammation
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PARTS OF GINGIVAL
EPITHELIUM
ORAL / OUTER EPITHELIUM
SULCULAR EPITHELIUM
JUNCTIONAL EPITHELIUM
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ORAL / OUTER EPITHELIUM
Covers crest & outer surface of marginal
gingiva & attached gingiva.
Majority of areas : Parakeratinized.
Degree decreases with age.
KERATIN PROTEINS :
Orthokeratinized – K1,K2,K10,K12.
Parakeratinized – K19
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Dr. Mayur Kaushik, Subharti Dental College, SVSU
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ORDER OF KERATINIZATION
PALATE
GINGIVA
TONGUE
CHEEK 21
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SULCULAR EPITHELIUM
Thin, non-keratinized squamous
epithelium without rete pegs.
Extends from coronal limit of junctional
epithelium to crest of gingival epithelium.
Lacks st. granulosum & st. corneum &
keratin proteins: K1,K2,K10,K12.
Contains K4 & K13.
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Dr. Mayur Kaushik, Subharti Dental College, SVSU
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KERATINIZATION OF SULCULAR
EPITHELIUM
Reflection & exposure to the oral cavity.
Complete elimination of bacterial flora
from sulcus.
Local irritation prevents sulcular
keratinization.
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CLINICAL SIGNIFICANCE
Acts as a semi-permeable membrane.
Bacterial products enter gingival tissues.
Tissue fluids from gingival tissues seep
into sulcus.
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GINGIVA MICROSCOPIC FEATURES
Dr. Mayur Kaushik
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GINGIVA : MICROSCOPY
LAYERS OF EPITHELIUM
PROCESSES : Proliferation /
Keratinization
CELLS : Keratinocytes & Non-
Keratinocytes
PARTS : ORAL , SULCULAR
EPITHELIUM
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JUNCTIONAL
EPITHELIUM
Collar-like band of stratified
squamous non-keratinizing epithelium.
Normal length : 0.25 – 1.35 mm (>500 μm)
Inflammation : 50 – 100 μm.
Formed by confluence of oral epithelium &
reduced enamel epithelium during tooth
eruption. 28
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PARTS OF JUNCTIONAL
EPITHELIUM
Attached to tooth surface –
INTERNAL BASAL LAMINA.
Attached to gingival connective tissue –
EXTERNAL BASAL LAMINA.
Junctional Epithelium + Gingival Fibers
DENTOGINGIVAL UNIT 29
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RENEWAL OF GINGIVAL
EPITHELIUM
Constant renewal.
Thickness maintained by new cell
formation in basal & spinous cells &
shedding on cell surfaces.
MITOTIC RATE
Buccal Mucosa, Hard Palate
Sulcular , Junctional Epithelium
Marginal , Attached Gingiva
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GINIGIVAL CONNECTIVE TISSUE (LAMINA PROPRIA)
PAPILLARY LAYER
Between epithelial rete pegs
RETICULAR LAYER
Continuous with periosteum
of alveolar bone.
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COMPONENTS OF CONNECTIVE
TISSUE
CELLS
EXTRACELLULAR COMPONENTS
GROUND SUBSTANCE
PROTEOGLYCANS – Hyaluronic Acid,
Chondroitin Sulfate
GLYCOPROTEINS – Fibronectin, Laminin
FIBERS
COLLAGEN (Type I)
RETICULAR (Type IV)
ELASTIC 32
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GROUPS OF GINGIVAL FIBERS
GINGIVODENTAL GROUP
TRANSSEPTAL GROUP
GINGIVOPERIOSTEAL
GROUP
CIRCULAR GROUP
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CELLULAR ELEMENTS
FIBROBLASTS : Principal cell type.
Synthesize as well as degrade collagen.
OTHER CELLS :
MAST CELLS
MACROPHAGES
ADIPOSE CELLS
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BLOOD SUPPLY
SUPRAPERIOSTEAL ARTERIES
VESSELS OF PERIODONTAL LIGAMENT
ARTERIOLES FROM CREST OF SEPTA
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LYMPHATICS
SUBMAXILLARY LYMPH NODES
NERVE SUPPLY
NERVES FROM PERIODONTAL LIGAMENT,
LABIAL, BUCCAL & PALATAL NERVES.
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