2015 LanzaTech. All rights reserved.

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GMOD powering LanzaTech

2016 GMOD Meeting

Indiana University, Bloomington, Indiana, United States

June 30 – July 1, 2016

Asela W. S. Dassanayake

Bioinformatics Research Scientist

Model Systems Group

asela.dassanayake@lanzatech.com

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• LanzaTech - Who we are and what we do

• How GMOD is powering the information flow at LanzaTech

• Proposing a Synthetic Biology platform?

The outline

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Ed Hawkins,Climate scientist,National Centre for Atmospheric Science,University of Reading.@ed_hawkins

65% of 2°carbon budget: USED

1870-2011: 1900 GtCO2

Remaining: 1000 GtCO2

Must stay in the ground

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Carbon smart world

Proprietary Acetogenic Biocatalyst

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Worlds’ first autotrophs

Acetogenic bacterium with ability to utilize gases as sole energy and carbon source – CO– CO+H2 or CO+CO2+H2

– CO2+H2

LanzaTech has developed a proprietary

strain of Clostridium autoethanogenum

Obtained by extensive selection program,

having improved characteristics– High gas uptake and ethanol production rates– Fast growth on defined minimal media– Non-sporulating and non-motile

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1 Organism, over 20 Products…

Pyruvate

CO/H2

Acetyl-CoA

Fatty Acids,

Terpenoids

Aromatics

Ethanol

Succinate

Lactate

2,3-Butanediol

(2,3-BDO)

Biodiesel (FAEE)

3-Hydroxypropionate

(3-HP)

Isopropanol

Acetone

3-Hydroxybutyrate

(3-HB)

1,3-Butanediol (1,3-BDO)

Partnerships

Butylene

1,3-Butadiene

Biopolymers

Branched-chain

Amino Acids

Butyrate

1-Butanol

Methyl Ethyl Ketone

(MEK)

2-Butanol

Acetoin

1,2-Propanediol

1-Propanol

Jet Fuel

Isoprene

Aromatics

DiscoveryContinuous

lab-scale

fermentation

Scaled

Up1 Organism, over 25 Products…

Acetate

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Piloting>50% selectivity

at lab scale

Gas Feed Stream

Gas Reception Compression Fermentation Recovery ProductTank

HydrocarbonFuels

Ethanol

Butanediol

Acrylonitrile

Butadiene

Styrene (ABS)

Nylon 6,6 (from Adiponitrile/HDMA)

PropanolButanol

The LanzaTech Process

Industrial Waste Gas

MSW WasteBiomass

We use:

Land Corn For People

We do not use:

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A sensible path

SugarCane

Strong Pipeline and Committed Partners

Commercial Production 2017

3 Commercial projects underway

ArcelorMittal, Gent

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Ground Works StartedOctober 2015

Gas Testing Station Produces Ethanol

January 2016

Connection to Steel Mill Gas Lines March 2016

1 Organism, over 20 Products…

Pyruvate

CO/H2

Acetyl-CoA

Fatty Acids,

Terpenoids

Aromatics

Ethanol

Succinate

Lactate

2,3-Butanediol

(2,3-BDO)

Biodiesel (FAEE)

3-Hydroxypropionate

(3-HP)

Isopropanol

Acetone

3-Hydroxybutyrate

(3-HB)

1,3-Butanediol (1,3-BDO)

Partnerships

Butylene

1,3-Butadiene

Biopolymers

Branched-chain

Amino Acids

Butyrate

1-Butanol

Methyl Ethyl Ketone

(MEK)

2-Butanol

Acetoin

1,2-Propanediol

1-Propanol

Jet Fuel

Isoprene

Aromatics

DiscoveryContinuous

lab-scale

fermentation

Scaled

Up1 Organism, over 25 Products…

Acetate

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Piloting>50% selectivity

at lab scale

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BIOENGINEERING

FERMENTATION

GENOMIC, TRANSCRIPTOMIC,

PROTEOMIC, METABOLOMIC DATA

BIOINFORMATICS, STATISTICS, MODELING

BIOCHEMISTRY, PATHWAY ANALYSIS

Information flow at LanzaTech

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GMOD at LanzaTech

External Collaborators

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Apollo at LanzaTech - Internal

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Transcriptional Regulator

Methyl accepting chemotaxis protein

Transcriptional Regulator

Uncharacterised ORF

Iron-Sulphur binding cluster

Roshomon

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Tuff-t

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Tuff-t

1 Organism, over 20 Products…

Pyruvate

CO/H2

Acetyl-CoA

Fatty Acids,

Terpenoids

Aromatics

Ethanol

Succinate

Lactate

2,3-Butanediol

(2,3-BDO)

Biodiesel (FAEE)

3-Hydroxypropionate

(3-HP)

Isopropanol

Acetone

3-Hydroxybutyrate

(3-HB)

1,3-Butanediol (1,3-BDO)

Partnerships

Butylene

1,3-Butadiene

Biopolymers

Branched-chain

Amino Acids

Butyrate

1-Butanol

Methyl Ethyl Ketone

(MEK)

2-Butanol

Acetoin

1,2-Propanediol

1-Propanol

Jet Fuel

Isoprene

Aromatics

DiscoveryContinuous

lab-scale

fermentation

Scaled

Up1 Organism, over 25 Products…

Acetate

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Piloting>50% selectivity

at lab scale

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• User data organization and management• BLAST Interface• Database Searching

• Literature searches and bibliography

• Genome browser

• Data Visualization

• Synthetic Biology CAD with SBML/SBOL

Proposing an Integrated Synthetic Biology platform

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Integrated Synthetic Biology platform

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More Users? Funding?