gram staining

Microbiology laboratoric test

Upload: yustin-putri-pratiwi

Post on 29-Oct-2014

51 views

Category:

Documents

7 download

Report

Download

Tags:

Embed Size (px):

TRANSCRIPT

Microbiology laboratoric test

Gram Stain Material

solutions of crystal violet, Gram’s iodine (2 g potassium iodide in 300 ml distilled water plus 1 g iodine crystals), 95% ethanol and/or

isopropanol-acetone mixture (3:1 v/v), and

safranin Bismark brown stain (for color-

blind students) clean glass slides inoculating loop Bunsen burner bibulous paper

microscope lens paper and lens cleaner immersion oil Hyphomonas

(Hyphomicrobium) neptunium (ATCC 15444) grown in marine broth (Difco)

slide warmer staining rack Bacto Gram Stain Reagents

from Difco for the three-step Gram stain

Gram Stain

Examination the preparation under microscope ( x100) for confirmation

Microscopic result for Gram Stain

Lab Report: Isolation of Pure Culture, Gram-staining, and Microscopic Observation

Gram Staining

Gram-Hucker’s Staining Kit

Prokaryotes (bacteria) and Gram Staining · Prokaryotes (bacteria) and Gram Staining Gram positive and Gram negative. BTEC101, Day 5 Why are we learning this? ... Single celled organisms

The Three-Domain System · . Gram positive cell wall Membrane protein. Gram negative cell wall 25. SIMPLE STAINING:- METHYLENE BLUE staining. IMPART SAME COLOUR TO ALL BACTERIA. USED

Ex. 3: Staining Techniques - Gram Stain, part 1 Chromophores Basic vs. acidic dyes

RelationshipofSanitationParameterswithMicrobial ... · appearance, size, elevation, form, edge, consistency, color, odor, opacity, and pigmentation. Gram staining, capsule staining,

Gram Staining Method

Gram Staining guest Lecture JIPMER Pondcherry

IFU GramPRO User Manual Page 1 51 - Hardy Diagnostics...safety precautions. 3.2. Background – Gram’s Staining Technique The Gram staining method, named after Hans Christian Gram,

CELL WALL - Journal of Bacteriologyresult in gram-positive staining, and failure of the dye to be present in the cell wall was the explanation of all gram-negative staining. Al-though

Gram Staining DR. SUSANTI 1

Francisella tularensis Tularemia. Francisella tularensis Gram stain –Poorly staining, tiny Gram-negative coccobacilli

Basic Microbiology Lab Reporting gram staining

Gram’s staining · Gram’s staining The Gram staining method is named after the Danish bacteriologist Hans Christian Gram (1853 – 1938) who originally devised it in 1882 (but

Observation of bacteria using staining procedures Simple staining Gram staining

A Flow-Cytometric Gram-Staining Technique for … 23 September 2002/Accepted 7 February 2003 A Gram-staining technique combining staining with two ﬂuorescent stains, Oregon Green-conjugated

Gram-Positive Irregular (shape and staining) Non- …Gram-Positive Irregular (shape and staining) Non-Spore-Forming Bacilli Acid fast bacilli Mycobacteria Non-Acid fast bacilli CorynebacteriaSchick

AIM To perform gram staining of given sample. MATERIAL ...davjalandhar.com/dbt/fst/SOP-Lab Manual/BFST SEMESTER - III.pdf · AIM : To perform gram staining of given sample. MATERIAL

Revised Gram Staining

Gram’s staining - Sridhar Raomicrorao.com/micronotes/pg/Gram stain.pdf · The original description of staining technique by Christian Gram in ... gentian violet and 5% phenol solution

2. pracical. motility, gram staining

Gram staining

1ISOLATION OF XYLAN DEGRADING MICROORGANISM ...umpir.ump.edu.my/481/1/2718.pdfmikroorganisma tersebut adalah berbentuk rod untuk ‘simple staining’, gram negatif untuk ‘gram staining’,

Introduction to Lab: Differential Stains Gram Staining

Gram staining and Clinical applications

Gram Staining of Bacteria - IISER Puneraghav/pdfs/bio121/Gram Staining.pdf · Gram staining is a bacteriological laboratory technique used to differentiate bacterial species into

Gram staining, Culture , Antimicrobial sensitivity test

Grams Stain-Kit · Only fresh cultures and specimens should be gram stained since cell wall integrity of older cells may give improper gram-staining characteristics. Gram positive

Gram Staining - WSDblog.wsd.net/tiswenson/files/2012/10/gram-stain.pdf · Allow the film to air dry. 1 3 2 ... Gram negative Comma form rods 1 2 . Gram Positive Spore-forming Bacilli

An optimized staining technique for the detection of Gram ... · the Gram stain incorporating a counterstain that highlights collagen found in tissue. The modified Gram stain clearly

3- Gram Staining

Safranin, 0.5% w/v - himedialabs.comhimedialabs.com/TD/S027.pdfSafranin is used as counter stain in Gram staining procedure to differentiate between gram positive and gram negative

Pewarnaan Gram (Gram Staining-hsc

Gram’s staininglibvolume6.xyz/.../stainsandstainingtechniquestutorial2.pdf · 2014-10-29 · 1 Gram’s staining The Gram staining method is named after the Danish bacteriologist

gram staining

Documents

gram stain

difco