grds international conference on health and life (19)
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Evaluation of wound healing activity of oral administration of ethanolic extract of Michelia champaca in diabetic wistar rats Shenoy S*, Gowda A, Shanbhag T, Prabhu K,
Nelluri V
*Department of Pharmacology, Kasturba Medical College, Manipal, India
Background
Wound healing - impaired in patients with diabetes
mellitus. Michelia champaca - family Magnoliaceae.
Flowers used by traditional healers for
ulcers, skin disease and wounds
Objective
To assess the effect of oral administration of ethanolic
extract of flowers of Michelia champaca on
healing of incision and excision wounds in
diabetic wistar rats.
Methods
Approval from Institutional Animal Ethics Committee obtained
Ethanolic extract of flowers of Michelia champaca
(MC) was prepared
Suspension of the extract made by using CMC 0.5% (carboxymethylcellulose)
Wound models
Incision wound
Excision wound
Animals
Five groups in each model
Six rats in each group
Total adult albino rats, wistar strain = 60
Group Drug, route
Group I (non-diabetic control)
CMC 0.5%, oral
Group II (diabetic control )
CMC 0.5%, oral
Group III (diabetic test)
100 mg/kg of Michelia champaca extract, oral
Group IV (diabetic test)
200 mg/kg of Michelia champaca, extract, oral
Group V (diabetic test)
400 mg/kg of Michelia champaca , extract, oral
Induction of diabetes
Streptozotocin (STZ) solution :
intraperitoneally - 30mg/kg
- four groups of rats
Incision wound
Two 6 cm long paravertebral straight incisions - through
entire thickness of the skin on the back of the rats.
closed with intermittent sutures
Parameter assessed
10th postoperative day - breaking strength of the wound –
continuous constant waterflow technique of Lee
Excision wound model Parameters assessed
Rate of wound contraction (%) =
initial wound size – specific day wound size
initial wound sizex 100
Period of epithelization
wound created on day 7 after induction of diabetes
anesthesia - ketamine (80 mg/kg) i.p.
round seal of 2.5 cm diameter - on the dorsal interscapular region, 5 cm away from the ears
full thickness skin from demarcated area excised
→ wound - 400 mm2 & 2 mm depth
Duration of drug administration
from day 0 (day of wounding)
to day 21 or
till wound was completely healed,
whichever occurred earlier
Histopathology
10th postoperative day,
granulation tissue from wound area –
using a punch biopsy needle (5 mm), under
ketamine anesthesia
Statistical analysis
one-way ANOVA followed by
Tukey Post-hoc test
Results
Effect of Michelia champaca on breaking strength of incision wound
Groups Drugs Breaking strength (g)
( Mean ± SEM)
Group ICMC 0.5%, oral
(nondiabetic control) 256.56 ± 6.54
Group IICMC 0.5%, oral(diabetic control) 222.61 ± 4.62*
Group III100 mg/kg Michelia
champaca extract, oral 238.17 ± 5.56
Group IV200 mg/kg of Michelia champaca extract, oral 245.78 ± 6.79
Group V400 mg/kg of Michelia champaca extract, oral 270.50 ± 4.40**
*p < 0.05 vs nondiabetic control ; **p < 0.05 vs diabetic control
Effect of Michelia champaca on healing
of excision wound
Groups Drug Period of epithelization - days ( Mean ± SEM)
Group I CMC 0.5%, oral(nondiabetic control)
18.86 ± 0.82
Group II CMC 0.5%, oral(diabetic control)
22.16 ± 0.45 *
Group III 100 mg/kg of Michelia champaca extract, oral
21.67 ± 0.22
Group IV 200 mg/kg of Michelia champaca extract, oral
20.72 ± 0.34
Group V
400 mg/kg of Michelia champaca extract, oral
19.31 ± 0.37 **
*p < 0.05 vs nondiabetic control ;
**p < 0.05 vs diabetic control
Groups Wound contraction (%) (Mean ± SEM) / drugs Day 4 Day 8 Day 12 Day 16
I- CMC 0.5% 18.62±3.66 40.53±2.47 60.88±5.21 81.24±4.12
II-CMC 0.5% 15.62±1.72 28.26±5.27* 43.92±3.76* 64.1±4.04*
III-MC extract100 mg/kg
17.45±4.23 31.57±1.45 49.28±5.46 70.97±3.13
IV - MC extract 200 mg/kg
18.36±4.64 35.60±3.26 52.32±4.10 73.77±5.26
V- MC extract 400 mg/ kg
21.86±2.70 37.97±4.87 62.94±3.48** 82.42±3.58**
*p < 0.05 vs nondiabetic control ; **p < 0.05 vs diabetic control
(c)
(e)
Discussion
Diabetic wounds – reduced
cellular infiltration, formation of granulation tissue,
collagen synthesis, breaking strength
Michelia champaca –
promoted better organization of collagen, reepithelization,
increased wound contraction rate, breaking strength
Michelia champaca – has
tannins, triterpenoids
antioxidant properties
antimicrobial actions
Conclusion
Ethanolic extract of Michelia champaca on oral
administration has a beneficial influence on wound
healing in diabetic rats
.