GREEN SYNTHESIS OF SILVER NANOPARTICLES AND THEIR ANTI-ACNE POTENTIAL Seema Garga and Amrish Chandrab

a Amity Institute of Applied Sciences, Amity University, Noida, 201301, Uttar Pradesh, Indiab Amity Institute of Pharmacy, Amity University, Noida, 201301, Uttar Pradesh, India

[1] R.G. Fried, A. Wechsler, Dermatol Ther 19 (2006) 237-240.[2] P. Mulvaney, Langmuir 12 (1996) 788–800.[3] R. Sathyavathi, M.B. Krishna, S.V. Rao, R. Saritha, D.N.

Rao, Adv Sci Lett 3 (2010) 1–6.

References*

Acne vulgaris or acne is the most common chronic

skin disease affecting nearly 80% of persons

between ages of 11 and 35 years [1]. Anti-acne

products have always been in great demand for

treatment of acne; however, formulations containing

synthetic drugs produce adverse effects.

Phytochemical constituents of black pepper were

employed for green synthesis of silver

nanoparticles. Aqueous solution of AgNO3 was

treated with black pepper corn extract and leading

to reduction of silver ions into silver nanoparticles

within 120 s with the aid of microwave. The

nanoparticles formed were characterized by UV-vis

spectrophotometer, TEM, XRD and FTIR [2]

Background1

Methods2

Results3

Conclusion• It is environmental friendly, simple and efficient.

• NP’s are spherical in shape with size range from 5 to 50 nm.

• AgNP’s - characterized using UV-Vis, XRD, TEM and FTIR spectroscopic techniques.

• Effective against S. epidermidis as compared to Clindamycin gel control & Extract

The synthesized silver nanoparticles were seen to be stable due to the presence of proteins which act as

capping agent and prevent the particle from aggregation. These nanoparticles were crystalline in

nature. These synthesized silver nanoparticles have various

applications in biomedical field, medical and pharmaceutical applications as well as large scale

commercial production.

5

Blank

Control

Extract

Silver nanoparticles

4 Data

Bacterial culture of S. epidermidis was incubated with clindamycin gel (control), black pepper corn extract (Extract) and silver nanoparticle synthesized using aquous extract of black pepper corn (nanoparticle)

0

0.5

1

1.5

2

2.5

3

3.5

250 300 350 400 450 500

Wave length(nm)

Abso

rban

ce

30Sec

60Sec

90Sec

120Sec

150Sec

210Sec

1 2 3 40

0.02

0.04

0.06

0.08

0.1

0.12

0.14

0.16

0.18

0.2

Blank Control Extract Nanoparticles

Time (hrs)

Abs

orba

nce