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1 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
GROUP II MAINS SAMPLE NOTES IN TAMIL
/ Bio Technology
Applications of Bio logical Organisms in
industries for human welfare’ , ,
,
, ,
.
(Medical or Red biotechnology)
.
, ,
, .
(Agricultural Or Green Bio -
Technology)
.
.
, .
( )
, ,
. ,
, , ,
. , ,
,
.
(Industrial OR White Bio Technology)
. ,
, ,
, ,
, , ,
.
2 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
- (Marine or blue Biotecnology)
,
, .
.
.
.
( , , , , , )
( , , )
( , , )
( )
.
MODERN BIOTECHNOLOGY
, ,
. , , ,
, , .
, , , ,
. ,
, , , ,
, ,
.
GMO
, IMF (Invitro Fertilization), , DNA
, .
(
, , ,
) .
,
DNA RECOMBINENT DNA TECHNOLOGY
( ) DNA
( ) DNA
.
3 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
DNA .
( TOOLS
USED IN GENETIC ENGINEERING)
•
•
• (Host Species)
• (Selectable Marker)
DNA - DNA
DNA (hybrid DNA)
.
DNA
1)
2) DNA
(1960)
4 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
DNA ( ) DNA
.
(1968)
.
(1970)
Hind II-
. 1978- .
1) .
2) .
3)
.
4) .
5) DNA
.
6) Hind II
(Heamophilous influenza) .
7) 230 900
.
8) )
Ex) (RY13)
ECORI (Escherisia Coli Restriction Endonuclease)
• E .
• CO
• R- –
• I - .
9) DNA- - .(S-P)
10)
.
5 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
11) 5' 3' .
12) DNA
. .
13) .
14) DNA
.
DNA :
T4 1969-
.
DNA
.
.
T4 DNA .
6 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
DNA
UV
DNA
.
7 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
DNA .
.
DNA
(Anode) .
DNA ,
UV .
DNA .
( ) ( )
( ) DNA
DNA
.
.
DNA
.
-
.
(Origin of replication) .
(Selectable Marker)
8 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
(Selectable Marker)
" ,
. "
DNA .
. ( , ,
, v
.
.
" DNA .
.
200 300 .
. 200 300
.
.
.
9 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
.
( DNA .
DNA .
.
.
45kb DNA .
.
.
BAC - (Bacterial Artificial Chromosome)
. .
300-350kb DNA .
. V
- 0.5mm
.
, .
" (Electric Field)
. "
DNA,RNA, ,
.
, ,
.
, ,
.
.
( ) ( )
10 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
DNA
. " , ,
. , , ,
,
.
DNA
1.
2. DNA DNA
.
3.
.
PCR
1985- .
PCR-
.
.
Y-
.
mRNA cDNA .
.
(DNA
.
. , ,
. (
.
DNA
.
11 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
.
.
,
.
.
.
.
(A
B ) C- C-
.
. A B
.
(S-S)
. .
1921-
.
(HGH)
.
(
) .
.
BGH
.
. BGH
.
.
.
. "
.
12 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
, , , ,
.
" .
(
) .
.
.
.
mRNA-
DNA or RNA .
, ,
mRNA-
.
HIV
.
DNA
.
, .
DNA
. (HBV) B
" .
.
.
13 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
rDNA
.
B
.
, HIV,
.
.
.
.
, , ,
.
TDNA PCR, ELISA
.
PCR
.
HIV PCR .
,
ELISA .
Probe
DNA .
Gene Therapy
. /
SCID, ,
.
. "
1990- .
14 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
.
.
.
.
.
, , ,
.
" . "
.
1997- " ”
(2.4 / ) .
.
( )
.
, , .
DNA - Finger Printing
VNTR - Variable Number of Tandem Repeats
VNTR DNA
.
. * 1858-
. ( 1984- .
DNA
DNA .
- , ,
DNA .
.
.
.
15 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
1.
(Diabetes mellifus)
2.
3. /
VIII/IX
4. TPA ( ) ,
5. PDGE (
)
6.
7.
.
8. B
9.
10. DNase I Cystic fibrosis
/
1) ( )
()
1. Cry I AC
2. Cry II Ab
1)
(
.)
2) 1. Cry I AC
2. Cry II Ab
.
3) 1) RNai (RNA
interference
Technology)
1)
16 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
2)
.
2) mRNA-
.
Slencing .
,
.
3)
Bt-
.
4)
RNA
.
.
2)
,
17 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
5) (B
)
1)
,
B
. Vit-
A
.
2)
.
6) 1) ADP
20
40
.
2) .
50
.
7)
1)
.
.
2)
,
,
18 GROUP II MAINS ONLINE CLASS ADMISSION OPEN -9952521550
.
(Biotechnology
Regulatory Authority of India, BRAI)
.
, , ,
.
Genetic Engineering Approval Committee - GEAC
,
1989- .
'
' (Genetic Engineering Approval Committee - GEAC)
Bio Informatics
.
1979
. , ,
,
,
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044-48601550, 9952521550
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