guidance of neuronal growth with dep
DESCRIPTION
Guidance of neuronal growth with DEP. J. James Jun Presenter University of Calgary Dr. Rudolf Potucek Supervisor University of Calgary January 25, 2007. Application of dielectrophoresis(DEP) for directing neuronal growth. Neuronal Guidance. Theory of DEP. Modeling & Experiment. - PowerPoint PPT PresentationTRANSCRIPT
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Guidance of neuronal growth with DEP
J. James Jun
PresenterUniversity of Calgary
Dr. Rudolf PotucekSupervisorUniversity of Calgary
January 25, 2007
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Application of dielectrophoresis(DEP) for directing neuronal growth
Neuronal Guidance
Theory of DEP
Modeling & Experiment
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Neuronal growth: random vs. directed
Random Directed
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Electric forces: Electrophoresis vs Dielectrophoresis
Electrophoresis
Any electric fields
Charged object
DC
Dielectrophoresis
Spatially varying field
Polarizable object
DC or AC
EPF qE
DEPF p E
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Modeling and Experiment
Microsphere
BrainPond snail Neuron
Field Modeling Vision Tracking
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Experiment with polymer bead
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Electric field modelingQuadruple electrodes configuration
top
side
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Computer vision tracking
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Experiment with a live snail neuronChallenges
More complicated than the experiment with beads
Long term experiment (3 days)
Need an evaporation control with perfusion system
Need a neurotrophic factor enriched medium
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Conclusions DEP can be used to direct a neuronal growth with electric field modeling and computer vision trackingDEP is a non-contact force and bio-chemically safe
Electric field modeling to optimizes DEP parameters
Computer vision tracking to track a growthcone
Experiments planned with beads then snail neurons
Questions?