h&e staining
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H&E STAINING. Lab Lec # 4. What is H&E ?. The hematoxylin and eosin stain ( H&E) Use in histology and histopathology laboratories. ability to demonstrate a wide range of normal and abnormal cell and tissue components Benefit! - PowerPoint PPT PresentationTRANSCRIPT
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H&E STAININGLab Lec # 4
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What is H&E ?• The hematoxylin and eosin stain (H&E)• Use
– in histology and histopathology laboratories.
• ability to demonstrate a wide range of – normal and – abnormal cell and tissue components
• Benefit!• it is a relatively simple stain to carry out on paraffin or frozen sections.• The first method of choice to determine whether a biopsy tissue is
cancerous or not.
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How it works?• Hematoxylin
– has a deep blue-purple color and – stains nucleic acids by a complex, incompletely understood reaction.– nuclei are stained blue,
• Eosin– is pink and stains proteins nonspecifically. – cytoplasm and extracellular matrix have varying degrees of pink staining.
– if abundant polyribosomes are present, the cytoplasm will have a distinct blue cast.
• Well-fixed cells show considerable intranuclear detail. Nuclei show varying cell-type- and cancer-type-specific patterns of condensation of heterochromatin (hematoxylin staining) that are diagnostically very important.
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Procedure
• Dewax – (paraffin wax –hyrdophobic/impervious to aqueous
reagents)• Hydrate – (aqueous reagents can penetrate)
• Add Hematoxylin– Add mordant (for binding)– Add alkaline solution (for more blueing)
• Apply Eosin• Dehydrate & Mount
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