HEMATOLOGY LABORATORY TABULATIONS

I. Plasma Coagulation Factors:1Factor IFibrinogen

2Factor IIProthrombin

3Factor IIIThromboplastin

4Factor IVCa+2

5Factor VProaccelerin

6Factor VIVa

7Factor VIIProconvertin

8Factor VIIIAntihemophilic Factor A

9Factor IXAHF B (Christmas factor)

10Factor XStuart Prower

11Factor XIPlasma thromboplastin antecedent

12Factor XIIHageman

13Factor XIIIFibrin stabilizing

II. Intrinsic, Extrinsic and Common Coagulation PathwayIntrinsic pathway: Reaction system that begins with factor XII and culminates in fibrin polymerizationScreened by: Activated partial thromboplastin time (APTT)Coagulation factors involved:1 XII2 Pre-K3 HMWK4 XI5 IX6 VIII7 X8 V9 Prothrombin10 Fibrinogen

Extrinsic pathway: Tissue factor pathwayScreened by: Prothrombin time test (PT)Coagulation factors involved:1 VII2 X3 V4 Prothrombin5 Fibrinogen

Common pathway: Common path of extrinsic and intrinsic pathwayCoagulation factors involved:1 X2 V3 Prothrombin4 Fibrinogen

III. Normal Values (CU-SI)

HemoglobinMaleFemale14-18 gm%12-16 gm%140 -180 gm/L120-150 gm /L

1gm Hb = 1.34 ml O2Specific gravity of blood = 1.0531gm Hb = 3.47 mg Iron1% Hct = 0.34 gm% Hb = 107,000 RBC / cu mm

HematocritMaleFemale42-52 vol%37-47 vol%0.42-0.52 0.37-0.47

RBC(x 10,000)MaleFemale4.5-6.0 M / cu mm4.05.5 M /cu mm4.5 -6.0 X 1012/L4.0-5.5 X 1012/L

WBC(x 50)5000 10,000 / cu mm5-10 X 109 /L

Differential countSegmented Neutrophil LymphocytesNeutrophilic band (stab or staff)MonocyteEosinophil Basophil60-70%25-35%2-6%

2-6%1-4%0.5-1%0.60 -0.700.25-0.350.02-0.06

0.02-0.060.01-0.040.005-0.01

ESRWintrobe:MaleFemale

Westergren:Male Female0-10 mm/hr0-20 mm/hr

0-15 mm/hr0-17 mm/hr

Absolute eosinophil count150-300 cells / cu mm

Reticulocyte count( 10)Adults Babies0.5-1.5%2.0-6.0%5-15 X 10-320-60 X 10-3

Platelet count(x 2,000 Rees-ecker mtd)(x 1000 Unopette mtd)200,000-400,000 cells / cu mm 200-400 X 109 /L

OFT(x 171) -> conversion factorGriffin SanfordInitial hemolysisComplete hemolysis

Dacies mtdInitial hemolysisComplete hemolysis0.42 -0.46%0.30-0.34%

0.50-0.55%0.35-0.40%72-79 mmol/L51-58 mmol/L

86-94 mmol/L60-68 mmol/L

Bleeding timeDukesIvys(2-3mm puncture)Copley lalitch (6mm puncture) O.O2-4 min1-7 min

4 petechiae at 200 mm Hg

IV. Tabulation of Methods

Blood collectionSkin punctureVenipuncture Syringe method Evacuated tube method

Smear preparationTwo slide or wedge methodTwo coverslips or Ehrlichs two cover glass methodSpinners methodAutomated method Sysmex SP-100

StainingStaining dish methodStaining jar or DIP methodAutomated method Hemastainer automatic slide stainer Hema-Tek 1000 slide stainer Hema-Tek 2000 slide stainer Sysmex SP-100 and Beckman Coulter

HemoglobinColorimetric methodsA. Direct method1 Tallquist or scale2 Dares hemoglobinometers3 Acid hematin method (Sahli hellige or Sahli adams)4 Alkali hematin

B. Photoelectric or indirect matching1 Oxyhemoglobin2 Cyanmethemoglobin or hemoglobin cyanide method3 Carboxyhemoglobin

Gasometric methodA. Van slyke oxygen capacity

Specific gravityA. Copper sulfate

Chemical methodsA. KenneysB. Wongs

Hemoglobin electrophoresis

HematocritMacro methodsA. WintrobeB. Hadens modificationC. Van allenD. Sanford magathE. Brays

Micro methodsA. Adams

Automated counterA. Coulter counterB. Autoanalyzer

ESRMacro methodsA. Wintrobe and LandsbergsB. WestergrenC. Graphic or cutlerD. LizenmeierE. Brays

Micro methodsA. Micro LandauB. Smith microC. Crista or hellige-vollmer

Automated methodsA. Mini VesB. Ves MaticC. Ves Matic 60

Absolute eosinophil countRandolph techniquePilot eosinophil countDirect eosinophil count by Friedman

Reticulocyte countMicroscopic methodsA. Dry methodB. Wet methodC. Miller disk method

Automated methodA. Sysmex R-1000

Platelet countDirect methodsA. Rees-EckerB. NygradsC. Guy and LeakesD. Brecker CronkiteE. Van allensF. Unopette

Indirect methodsA. DameshekB. FoniosC. Olefs (best indirect)

OFTRapid Screening testDacies methodGriffin Sanford methodQuantitative testUnopette method

Bleeding timeDukes Ivys (2-3mm puncture)Copley lalitch method (6mm puncture) O.O

Clotting timeCapillary blood methodsA. Drop or slideB. Capillary tube or Dale and Laidlaws

Whole blood or Lee and White Method

Clot retraction timeQualitativeA. Castor oil or Hirschboeck method

QuantitativeA. Stefanini or test tubeB. Mac farlane

Capillary resistance testTourniquet or Rumpel-Leedes or HessSuction or Petechiometer

V. Diluting Fluids

RBCDacies fluid or Formol citrate (best rbc diluting fluid)HayemsGowersToissonsBethellsNSS3.8% Sodium citrate

WBC1-3% Acetic acid with gentian violet1% HClTuerks

Absolute eosinophil countPhloxinePilotRandolphhs

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