heterogeneity in human hippocampal camkii transcripts ... · 1 heterogeneity in human hippocampal...
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HeterogeneityinhumanhippocampalCaMKIItranscriptsrevealsanallostericroleforthehubdomaininactivityregulationRomanSloutsky*1,NoelleDziedzic*1,2,MatthewJ.Dunn1,RachelM.Bates1,AnaP.Torres-Ocampo1,2,BrendanPage1,JohnG.Weeks1,MargaretM.Stratton1DepartmentofBiochemistryandMolecularBiology1,MolecularandCellularBiologyGraduateProgram2,UniversityofMassachusetts,Amherst,UnitedStates*TheseauthorscontributedequallyABSTRACTCa2+-calmodulindependentproteinkinaseII(CaMKII)playsacentralroleinCa2+signalingthroughoutthebody.Specificallyinthehippocampus,CaMKIIisrequiredforlearningandmemory.CaMKIIisencodedbyfourhighlyconservedgenesinvertebrates:α,β,γ,andδ.AllCaMKIIsarecomprisedofakinasedomain,regulatorysegment,variablelinkerregion,andhubdomainresponsibleforoligomerization.Thefourgenesdifferprimarilyinlinkerlengthandcompositionduetoextensivealternativesplicing.Numerousquestionsregardingthesesplicevariantsstillremain,startingwiththefundamentalquestionofwhichvariantsareexpressedandhowsplicingaffectsCaMKIIregulation.Here,weunambiguouslyreporttheheterogeneityofCaMKIItranscriptsinacomplexsampleofhumanhippocampususingIlluminasequencing.Ourresultsshowthathippocampalneuronscontainadiversecollectionof56CaMKIItranscriptsfromallfourCaMKIIgenes,including25thathavenotbeenpreviouslyannotated.WecharacterizedtheCa2+/CaMsensitivityofhippocampalCaMKIIvariantsspanningabroadrangeoflinkerlengthsandcompositions.WedemonstratethattheeffectofthevariablelinkeronCa2+/CaMsensitivityisconditionalonkinaseandhubdomains.Moreover,werevealanovelroleforthehubdomainasanallostericregulatorofkinaseactivity,whichmayprovideanewpharmacologicaltargetformodulatingCaMKIIactivity.Takentogether,weproposethatCa2+/CaMsensitivityinCaMKIIisgene-dependentandincludessignificantcontributionsfromthehub.OursequencingapproachcombinedwithbiochemistryprovidesnewinsightsintounderstandingthecomplexpoolofendogenousCaMKII.SIGNIFICANCECaMKIIisawell-conservedproteinthatisessentialforlearningandmemory.WhenCaMKIIismutatedinamouse,thismousehasdifficultylearningandrememberinghowtogetthroughamaze.Thehippocampusisthepartofthebrainrequiredformemory.Here,weusedaspecificexperimenttodetermineeverytypeofCaMKIIthatisinahumanhippocampus.Wefound56differenttypesandthenaskedhowthesedifferencesaffectCaMKIIfunction.ThesedataprovideevidencethatanunexpecteddomainofCaMKIIisimportantforregulatingitsactivity.ThisnewfindinghelpsusbetterunderstandendogenousCaMKIIinthebrainandprovidesanewmechanismformodulatingCaMKIIactivity.
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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INTRODUCTIONCa2+/calmodulindependentproteinkinaseII(CaMKII)isacrucialoligomericSer/Thrkinaseinlong-termmemoryformation(1)aswellaseggactivationinfertilization(2,3),andcardiacpacemaking(4).CaMKIIisexpressedthroughoutthehumanbody,whichfacilitatesitsroleinthesedisparatefunctions.TherearefourhumangenesthatencodeCaMKII:CaMKIIαandβ(predominantlyinbrain),CaMKIIδ(predominantinheart)andCaMKIIγ(multipleorgansystems,includingeggandsperm)(5-7).Inallfourgenes,eachCaMKIIsubunitiscomprisedofakinasedomain,regulatorysegment,variablelinkerregion,andahubdomain(Fig.1A).Thekinaseandhubdomainsofallfourgenesarehighlyconservedwithminimum90%and75%pairwiseidentity,respectively.Thelinkerthatconnectsthekinaseandhubdomainsishighlyvariableinlengthandcompositionduetoalternativesplicing.Herein,wefocusonCaMKIIinthehippocampus,whichcomprisestheprimarymemorycenterinthebrain(8).CaMKIIisthemostabundantenzymeinneuronaldendritesandhasbeenimplicatedfunctionallyinmemory(1).TransgenicmicedeficientinneuronalCaMKIIhavelimitedlong-termmemoryanddisplayspecificlearningimpairments(9,10).Morespecifically,micewithmutationsatcriticalphosphorylationsitesinCaMKIIdisplaydefectsinhigherordermemoryandadaptivelearning(11).ThestructureandfunctionofboththekinaseandhubdomainsofCaMKIIhavebeenwellstudied.Kinaseactivityiscontrolledbytheautoinhibitoryregulatorysegment,whichblocksthesubstrate-bindingsiteintheabsenceofCa2+(12).WhenCa2+levelsrise,Ca2+boundcalmodulin(Ca2+/CaM)competitivelybindstotheregulatorysegmentandrelievesinhibitionbyexposingthesubstrate-bindingsite(Fig.1B)(13).Theregulatorysegmentalsohousesthreesitesofautophosphorylation:Thr286,Thr305,Thr306(followingCaMKIIαnumbering)(14).Notably,phosphorylationatThr286resultsinautonomousactivation,or,sustainedactivityintheabsenceofCa2+(15).Thehubdomainoligomerizestheholoenzymeintobothdodecamericandtetradecamericassemblies(Fig.1C)(16-18). Thevariablelinkerregionhasbeendifficulttostudyforseveralreasons.Thesequenceofthelinkerispredictedtobedisordered.Itislikelyforthisreasonthathigh-resolutionstructuresoftheCaMKIIholoenzymeincludingthelinkerregionhavenotbeensolved.Indeed,theonlyatomicresolutionstructureofaCaMKIIholoenzymeisoneinwhichthevariablelinkeriscompletelydeleted(19).Inthisstructure,theregulatorysegmentmakesextensivecontactswiththehubdomain,therebycompletelysequesteringtheCaMbindingsegmentfromCa2+/CaMinthiscompactconformation.Thisstructure,combinedwithbiochemicaldatadiscussedbelow,ledtothehypothesisthatlinkerlengthmaytunetheautoinhibitionwithintheholoenzymebyeitherstabilizingacompactconformation(shortlinkerforms)orpopulatinganextendedconformationwheretheCaMbindingsegmentismoreaccessible(longerlinkerforms).ThevariablelinkeralsovariesincompositionbetweenthefourCaMKIIgenes.Furthercomplexityisgeneratedfromalternativesplicingupto9exons(ex13-21)ineachlinker,creatingatotalofmorethan50variantspossiblyexpressedfromfourgenes(Fig.2A).Thecombinationofhighidentitywithintheconservedkinaseandhubdomainsandhighvariability
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inthelinkerregionmakesitdifficulttospecificallyidentifysplicevariantsofparalogsincellularexperiments.Therehavebeenmanystudiesthatoutlinespecificrolesforexonsinthevariablelinkerregion,whichprovidesomecluesastowhyexonsmaybesplicedinorout.Exon15,whichisencodedinallgeneswiththeexceptionofCaMKIIβhasbeendocumentedtocontainanuclearlocalizationsequence(NLS)(20-22).ThisNLS(KKRK)isacommonmotifidentifiedasasufficientsequencetoachievenuclearlocalization(23).Exon13,whichisencodedinCaMKIIβandγ,butnotαorδ,mediatesanimportantinteractionwithF-actin(24,25).Indendriticspines,CaMKIIinteractingwithactiniscrucialforthemaintenanceofspinestructureduringlong-termpotentiation(26).PerhapsthemostdramaticformofalternativesplicingresultsintheformationofalphaCaMKIIassociatedprotein(alphaKAP)whereCaMKIIαissplicedtoaformthatincludesonlythehubdomain(27).ThissplicevariantplaysanimportantroleinmembraneanchoringusingitshydrophobicN-terminaldomain(28).Finally,itwasrecentlyshownthatalternativesplicingwithinthehubmightregulateoligomerization(5).Rolesfortheremainingexonshaveyettobedelineated.Inadditiontorolesassignedtospecificexons,thevariablelinkerregionhasalsobeenshowntoplayacriticalroleinCaMKIIactivation.AstandardmetricforCaMKIIactivityistheconcentrationofCa2+/CaMrequiredforhalf-maximalactivation(EC50value)(19,29).IthasalsobeenclearlyshownthatCaMKIIactivationiscooperative(29).Inregardtolinkerlength,aCaMKIIαvariantwitha30-residuelinkerhasbeenshowntorequiresignificantlylessCa2+/CaMtoachievemaximalactivitycomparedtoaCaMKIIαvariantwitha0-residuelinker(19).ThisgeneraltrendisalsotruewhencomparingalonglinkerCaMKIIβvarianttoashortlinkerCaMKIIαvariant(30).Here,weaddressthegapsinknowledgeintwomajorareas:1)whichspecificCaMKIIsplicevariantsareexpressedinhumanhippocampalcells,and2)howCaMKIIactivationisregulatedwithintheholoenzymestructureforotherCaMKIIgenes.RESULTSTheexonarchitectureisconservedbetweenCaMKIIparalogsThefourgenesencodingCaMKIIinhumansshareahighdegreeofhomology.UsingtheNeighborJoiningmethod(31,32),wecreatedreconstructionsofthekinaseandhubdomaindivergenceforthefourhumanCaMKIIgenesrootedwithD.melanogasterCaMKIIkinaseandhuboutgroups(Fig.1D).Becauseoftheclearone-to-onecorrespondenceofexonsacrossgenes,weemployacommonexonnumberingsystem(Fig.2A).Herein,weimplementanewnamingschemeforCaMKIIvariantsbasedongeneidentityandincorporatedexons(Fig.2C).Movingforward,weproposethisisthesimplestwaytoefficientlycatalogcurrentCaMKIIvariantsandincludenewvariantsastheyarediscovered.Deepsequencingrevealsatleast56CaMKIItranscriptvariantsinhumanhippocampusWeusedIlluminasequencingtounambiguouslydeterminetheCaMKIItranscriptspresentinahumanhippocampussample.
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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StrategyofamplificationanddetectionofspecificCaMKIIvariantsInordertoidentifyallalternativelysplicedCaMKIItranscriptsexpressedinahumantotalRNAsample,thesetranscriptsmustbedramaticallyenriched.Similartoearlierapproaches(1,3,(33-35),wereverse-transcribedhumanhippocampalRNAtogeneratecDNAandthenusedgene-specificprimerpairstoPCR-amplifyvariableregionsofCaMKIItranscripts(Fig.2B).AgarosegelseparationofPCRampliconsfollowedbySangersequencingofexcisedgelbandswasinadequatefordetailedcharacterizationofalternativeCaMKIIsplicing,becausemanybandscontainedmixturesofvariants.Tocircumventthisissue,wedevisedanIlluminanext-generationsequencingapproach.GiventhelocationsatwhichCaMKIItranscriptsarevariablysplicedandthemaximallengthsofvariableregionswhenalloptionalexonsareincorporated,strategicplacementofaprimerpairfacilitatesPCR-basedconstructionofIlluminasequencinglibrariessuchthata251bppaired-endsequencingstrategyallowsunambiguousidentificationofvirtuallyallpossibleCaMKIItranscriptsfromallfourgenes(Fig.2B,blue/greenprimerpair).Comparedtotraditionalwhole-transcriptomeIlluminaRNA-Seqlibrarypreparation,thisapproachemphasizessensitivityofvariantdetectionoveraccuracyinquantificationofrelativetranscriptabundance,duetothegreaterpotentialforamplificationbiasresultingfromadditionalPCRcycles.Therefore,wewillnotdiscusstranscriptabundance,excepttosaythateachidentifiedvariantissupportedbyfartoomanyreads(50ormore)tobeattributabletosequencingerrors.AnalysisofIlluminasequencingWedetectedasurprisinglylargenumberofdistincttranscriptvariants(Fig.2C,Supplement1,2),including3variantsofCaMKIIα,24ofCaMKIIβ,17ofCaMKIIγ,and12ofCaMKIIδ.Inadditiontovariantspreviouslyannotatedingenomicdatabases(NCBIConsensusCDScollection,NCBIGenBank,Ensembl),wealsoidentifiednumerouspreviouslyunobservedvariantsofeachgene,whichwerefertoasnovelinthismanuscript.OnenovelvariantofnotewastheCaMKIIαtranscriptincludingnooptionallinkerexons(0aalinker).ThisissignificantbecausethecrystalstructureofCaMKIIαno-linkervariantistheonlyavailablestructureoffulllengthCaMKII(PDB:3SOA(19)),butthisvariantwaspreviouslynotknowntobebiologicallyrelevant.Nearlyeveryidentifiedtranscriptincorporatingoptionallinkerexonscontainedexons14and18,suggestingthesetwoexonsconstitutea“core”linkerpredatingthedivergenceofthefourgenes.Assumingthecoreismandatoryifanyotherlinkerexonsaretobesplicedin,weobservedalargefractionofallowablelinkersequencesforeachgene.CaMKIIβvariantsincorporatingproline-richexons19,20,or21andCaMKIIγvariantsincorporatingF-actin-interactingexon13,whichwerenotdetectedinourexperiment,aretwonotableexceptions.ThevariablelinkeraffectsactivationofCaMKIIα,butnotCaMKIIβWefocusedontheactivationpropertiesofCaMKIIαandβvariantsbecauseoftheirwell-documentedinvolvementinlearningandmemory(9,11).First,wedeterminedtheEC50valuesforCa2+/CaMactivationofallthreeCaMKIIαvariantssequencedfromhumanhippocampus.ForallEC50measurements,thecurvefitisshowninFigure3forsimplicity,andfitswithalldata
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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pointsusedareshowninSupplement3.Forbrevitywerefertovariantsbylinkerlength:α-41(α14,15,18inFig.2C),α-30(α14,18),andα-0(α-no-linker).Inagreementwithpreviousexperimentscomparingα-30toα-0(19),wealsoobservedasignificantright-shiftintheEC50value(from24nMto313nM,respectively,Fig.3A).CaMKIIα-41(EC50=25nM,Fig.3A)didnotshowasignificantdifferencecomparedtoα-30.Next,weexaminedCaMKIIβvariantswiththebroadestpossiblerangeofvariablelinkerlengths:previouslyannotatedanddetectedvariantsβ-93,β-69,β-68,β-54,β-30,andβ-0,aswellastheannotated,butundetected,variantβ-217(Figure3B).Quitesurprisingly,weobservednosignificantdifferencesinEC50acrosstheentirerangeoflinkerlengths,withEC50ofallβvariantsfallinginthenarrowrangebetween14-31nM.ThisresultshowsthatthevariablelinkerdoesnottuneactivationthesamewayindifferentCaMKIIgenes.Thehillcoefficients(nH)fromthesemeasurementsofCaMKIIαandβvariantswereallquitesimilar,rangingfrom1.5to2.5,whichisconsistentwithpreviousmeasurements(19).Ofnote,thecooperativityinCaMKIIβvariantsisconsistentlyhighercomparedtoCaMKIIα,butdoesnotcorrelatewithEC50valuedifferences.Weobserveda15-folddifferenceintheEC50forCa2+/CaMbetweenα-0andβ-0(313nMvs21nM).Thedifferenceinactivationpropertiesofthesetwovariantsmustbedeterminedbysequencedifferencesoutsideofthevariablelinker:inthekinasedomain,thehubdomain,oracombinationofthetwo.Infact,theonlyCaMKIIβvariantwetestedwithanEC50outsideofthenarrowrangearound20nMwasthenovelvariantβ-93-short-hub,whichisslightlyright-shiftedfromtheotherCaMKIIβvariants(Fig.3B).Theonlydifferencebetweenβ-93(25nMEC50)andβ-93-short-hub(43nMEC50)are26residuesmissinginthehubdomain,indicatingthathubalonemayaffectactivationproperties.Theseresultsmotivatedournextexperiments.ThehubisdominanttothekinaseandvariablelinkerindeterminingactivationpropertiesWedirectlyassessedtheregulatorycontributionofthekinaseandhubdomainsofCaMKIIαandβ(90%and77%identity,respectively).Todothis,wecreatedtwochimeraswith0aalinkers.ThefirstchimeraistheCaMKIIαkinasedomainfusedtotheCaMKIIβhubdomain,andthesecondchimeraistheCaMKIIβkinasedomainfusedtotheCaMKIIαhubdomain.TheEC50valueoftheβkinase/αhubchimerawassignificantlyright-shiftedcomparedtothatoftheαkinase/βhubchimera(Fig.3C).BothCaMKIIβ-0andthechimerawiththeβhubhaveroughlythesameEC50(24nMand21nM,respectively).Ontheotherhand,CaMKIIα-0andthechimerawiththeαhubarebothright-shifted.However,theEC50ofthechimeraisstill3-foldlowerthantheEC50ofwildtypeCaMKIIα-0:102nMvs313nM.Thissuggeststhattheroleofthehubdomainindeterminingactivationpropertiesisdominantwithrespecttothekinasedomain,withnocontributionfromthekinaseinthecontextofβhub,butasignificantcontributioninthecontextofαhub.ActivationpropertiestrackwithevolutionarydivergenceWecomparedthedivergenceofhumanCaMKIIkinaseandhubdomainsusingthecorrespondingdomainsfromthesingleCaMKIIgeneinflyasoutgrouptorootthetrees(Fig.
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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1D).Thekinaseandhubtreeshaveconsistenttopologies,inwhichthecommonancestorofCaMKIIαandδdivergedfromthecommonancestorofβandγ.However,whilethefourkinasedomainsdivergedinnearlyastartopology(thesegmentsseparatingtheα/δandβ/γancestorsfromtheircommonancestoraremuchshorterthananyothersegment),hubdivergencewasnotnearlyasuniform.CaMKIIαandδaremuchmoredivergedfromeachotherandfromβ/γ.CaMKIIβandγhubshavedivergedbyfartheleast,withthefewestsubstitutionsbetweenthem.Basedonthissimilarity,andtheapparentinvolvementofthehubdomaininactivation,wehypothesizedthatγ-0wouldbehavesimilarlytoβ-0.Wemeasuredactivationofγ-0andδ-0(Fig.3C),confirmingourhypothesis(γ-0:EC50=28nM).Consistentwiththeorderinwhichα,β/γ,andδsplit,aswellaswiththegreaterdegreeofdivergencebetweenαandδhubs,δ-0requiredanintermediateamountofCa2+/CaMforactivation(EC50=167nM):roughly7-foldhigherthanβ-0,but2-foldlowerthanα-0.DISCUSSIONForthefirsttime,weunambiguouslydeterminedatleast56CaMKIItranscriptspresentinahumanhippocampalsampleusingIlluminasequencing.Usingthesesequences,andotherspreviouslyannotatedfromvarioustissues,weaskedwhatarethedifferencesinregulationbetweenCaMKIIgenesandsplicevariants.WediscoveredthatwhileCaMKIIαregulationisdependentonthevariablelinkerregion,CaMKIIβisnot.Further,wenowshowanovelroleforthehubdomainintheregulationofCaMKIIactivationbyCa2+/CaM.OurdataprovideimportantinsightsintotheheterogeneityofCaMKIItranscriptsinahumantissueandalsoimplicateanewroleforthehubdomaininregulatingCaMKIIactivitythroughanallostericmechanism.ThisfindingopensanewwindowtoallostericcontrolofCaMKIIactivitythroughmodulationofthehubdomain.WesequencedasignificantnumberoftranscriptsfromallfourCaMKIIgenes.Notably,CaMKIIαproducedano-linkertranscript(CaMKIIα-0)thataccountedforover3%ofmappedreads.Thissequencewaspreviouslycrystallizedtoobtaintheonlyfull-lengthatomicresolutionstructureofCaMKII.Ourdatademonstratethepotentialphysiologicalrelevanceofthecompactconformationobservedinthatstructure(19).Thesheernumberofdetectedvariantsinhippocampalneurons(56acrossthefourCaMKIIgenes)suggeststhateithersplicingisnottightlyregulatedateveryexon,or,conversely,therearecomplexmechanismsofregulation.Intermsofabundanceoftranscriptswedetected,PCRbiasisunlikelytobesolelyresponsiblefortheroughly1000-folddifferencesbetweenthemost-andleast-frequentlyobservedvariantsofCaMKIIβ,γ,andδ.Thissuggeststhatsomevariantsaredeliberatelyup-regulatedrelativetoothers.Furthermore,wedidnotdetectevenasinglereadmappingtosomeofthepreviouslyannotatedvariants(forinstanceCaMKIIβvariantsincorporatingexon19,20,or21,andCaMKIIγvariantscontainingexon13).Thissuggeststhatsomevariantsarespecificallydown-regulated.WefoundthatagarosegelseparationoftranscriptsfollowedbySangersequencingofindividualbandscannotresolveCaMKIIvariantsfromhippocampus(Supplement4).Thisis
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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becausesequencingtracesfrommostbandsclearlyindicatedamixtureoftemplatespresentinthesequencedsample.Inmanycases,carefulanalysisoftheSangersequencingtraceallowedustoidentifyatleasttwodistinctsplicevariants.Therefore,itisdifficulttointerpretthepresenceorabsenceofanexpectedgelbandasindicativeofthepresenceorabsenceofaparticularCaMKIItranscriptinasample.SinceeachIlluminareadobtainedbyourapproachreflectsasingletemplatemolecule,itproducesasignificantlymorerobustsurveyoftheCaMKIItranscripts.WesuggestthatthisapproachshouldbeappliedtoothertissuestobegintocatalogthediversityinCaMKIItranscripts.Weexpectthatinothertissuestheidentityandrelativeabundanceoftranscriptswillvaryfromthoseweobservedinthehippocampus.Withthisinformationinhand,wewillcertainlyimproveourunderstandingoftheregulationofalternativesplicing,andultimatelybeabletolinkthisregulationtothebiologicalneedswithineachtissue.Finally,ourresultscomparingtheEC50valuesbetweenCaMKIIαandβsplicevariantsshowthattheregulationofCaMKIIactivationisgene-dependentandsignificantlyaffectedbythesequenceofhubdomain.ThevariablelinkerinCaMKIIαdoesplayasignificantroleinregulatingCaMKIIαactivity,whichisconsistentwithpreviouswork(19).WeusedcartoonholoenzymesinFigure3toillustratethecompactandextendedautoinhibitedstatesofCaMKII,whichcorrespondtolowerandhighersensitivitiestoCa2+/CaM.ForCaMKIIα,thesecartoonsarebasedonacrystalstructure(PDB:3SOA)andSAXSmeasurements,respectively(19).Surprisingly,thevariablelinkerinCaMKIIβdoesnotplayasignificantroleinregulatingCaMKIIβactivity.UsingtheseEC50measurements,wehavedescribedCaMKIIactivationatfixedCa2+/CaMconcentrations.CaMKIIactivityisalsodependentonthefrequencyofCa2+delivered(19,30,36).Futureexperimentswillbeneededtocarefullydissectthiskineticcontributionindifferentsplicevariants.OurresultswithCaMKIIαandβchimerasprovideevidencethatthehubistheprimarydeterminantofsensitivitytoCa2+/CaM.AnalysisoftheCaMKIIαholoenzymecrystalstructureshowsthatthesubstitutedresiduescomparingCaMKIIαandβhubdomainsaredistributedfairlyevenlythroughoutthedomain(Fig.4A).Noneofthesesubstitutionsarefoundattheinterfacebetweenthehubandregulatorysegment,whichwouldbecleartointerpret.TheextremeC-terminusdoesvaryslightlyfromgenetogene(SVLPHinCaMKIIα),whichmaybeimplicatedinoligomerizationeffects(Fig.4B).Takentogether,wehypothesizethatthiseffectofthehubonkinaseactivityisbasedondynamics;wherebreathingmotionsorstabilitywithinthehubdomainallostericallyregulatestheaccessibilityofthecalmodulin-bindingsegment.Furtherexperimentswillbeneededtofullyelucidatethiscomplexmechanism.MATERIALSANDMETHODSInitialamplificationoftranscriptvariableregionscDNAwasreverse-transcribedfromacommerciallypurchasedhumanhippocampalRNAsample(BioChain)usingtheProtoScriptIIfirststrandcDNAsynthesiskit(NEB#E6560)witholigodTpriming.VariableregionsoftranscriptswerePCR-amplifiedfor35cyclesusinghigh-specificityprimerpairsdesignedtominimizecross-hybridizationbetweenCaMKIIgenesand
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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Phusionpolymerase(NEB#M0530).Thisyieldedamixedpopulationofvariable-lengthampliconsreflectingthesplicevariantsofthetargetedgenepresentintheRNAsample.ConstructionofIlluminaSequencingLibrariesIlluminasequencinglibrarieswerepreparedfromtheinitialvariable-lengthampliconpoolsusinganadaptedPCR-basedconstructionprotocol(37).Briefly:first,gene-specificprimerswithoverhangscontainingIllumina-specifiedsequences(Figure2B)wereusedtosub-amplifytranscriptvariableregions.Second,genericprimersannealingtotheoverhangsintroducedinthefirstreactionwereusedtoincorporateannealingsitesforIlluminasequencingprimers,NEBNext(NEB#E7335)libraryindices,andP5/P7flowcellannealingsequences.Theresultingampliconswereagarosegel-purifiedwithsizeselectionbasedonthetheoreticalminimalandmaximallengthsofthevariableregioninserts.AnalysisofIlluminaSequencingIlluminareadsweregroupedbysequencesimilarity,producingclusterswith>90%sequenceidentityandtwoorfewerbasepairinsertions/deletions.TheconsensussequenceofeachclusterwasalignedtoexonsequencesfromthecorrespondingCaMKIIgeneobtainedfromthereferencehumangenome(GRCh38.p12,Ensemblrelease95)inordertomapexonorderinthetranscriptrepresentedbythecluster.Over90%ofreadsfromeachIlluminalibrarycouldbeclusteredandunambiguouslymappedwithreferenceexons.Weconsiderthese“mappable”reads.Python2codeimplementingreadclusteringandexonmappingisavailableuponrequest.ExpressionplasmidconstructionFull-lengthCaMKIIvariantswereclonedintoapETvectorcontainingN-terminal6xHisfollowedbyaSUMOtag.CaMKIIchimeraswereconstructedasfollows.Thekinase/regulatorysegmentandhubdomainsofCaMKIIαandCaMKIIβwereamplifiedfromfull-lengthconstructs.Thekinasedomainincludestheregulatorysegmentofitscorrespondingkinase.Eachdomainwasamplifiedwitha20bpsoverhangthatwasthecorrespondingCaMKIIgene.ThechimeraswereassembledusingtheGibsonassemblytechnique.ProteinexpressionandpurificationAllCaMKIIvariantsusedforthisexperimentwererecombinantlyexpressedandpurifiedaccordingto(38).PlasmidsencodingCaMKIIvariantswereco-transformedwithlambdaphosphataseintoRosetta2(DE3)pLysScompetentcells(Millipore).Expressionwasinducedovernightwith1mMIsopropylβ-D-1-thiogalactopyranoside(IPTG)andculturesweregrownovernightat18°C.CellpelletswereresuspendedinBufferA(25mMTris-HClpH8.5,150mMKCl,50mMImidazole,10%glycerol)with25mMMagnesiumchloride,containingacocktailofproteaseinhibitorsandDNAse(AEBSF0.2mM,Leupeptin0.005mM,1ug/mLPepstatin,1ug/mLAprotinin,0.1mg/mLTrypsinInhibitor,0.5mMBenzamidine,1ug/mLDNAse)andlysed.AllsubsequentpurificationstepswereperformedusinganÄktapurechromatographysystemat4°C.Clarifiedlysatewasloadedontoa5mLHisTrapFFNiNTASepharosecolumn(GE),andelutedwithacombinationof50%BufferAand50%BufferB(25mMTris-HClpH8.5,
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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150mMKCl,1Mimidazole,10%glycerol)forafinalconcentrationof0.5Mimidazole.TheproteinwasdesaltedofresidualimidazoleusingaHiPrep26/10Desaltingcolumn,andHisSUMOtagswerecleavedwithUlp1proteaseovernightat4°CinBufferC(25mMTris-HClpH8.5,150mMKCl,2mMTCEP,50mMImidazole,10%glycerol).CleavedtagswereseparatedbyasubtractiveNiNTAstep.Next,ananionexchangestepwasdoneusinga5mLHiTrapQ-FFandproteinwaselutedwithaKClgradient.Elutedproteinswereconcentratedandfurtherpurifiedingelfiltrationbuffer(25mMTris-HClpH8.0,150mMKCl,1mMTCEP,10%glycerol)usingaSuperose6Increase10/300sizeexclusioncolumn(GE).Purefractionswerethenconcentrated,aliquoted,flashfrozeninliquidnitrogenandstoredat-80°Cuntilfurtheruse.Calmodulin(Gallusgallus)wasrecombinantlyexpressedfromapET-15bvector(generousgiftfromAngusNairn)inBL21(DE3)cells(Millipore)andpurifiedaspreviouslydescribed(PutkeyandWaxham,1996).Toquantifythecalmodulinconcentrationformakingstockstouseinthekinaseassays,weusedcirculardichroismonaJascoJ-1500Spectrophotometertomakeameasurementintriplicateforourpurifiedsamplescanningawavelengthspectrumbetween250-215nmtomeasurethecharacteristicwavelengthof222nmaccordingto(39).Equationusedtocalculatecalmodulinconcentration:[CaM](mM)=(1000*(mdegat222nmsample-mdegat222nmblank))/(ϴ*ℓ*(#aminoacids))ℓ=pathlength,ϴ=molarellipticity
calmodulin nM = 1000 × (𝐶𝐷!"#$%&!!!!" − 𝐶𝐷!"#$%!!!!")Θ × 𝑙 × number of amino acids
wherecirculardichroismat222nm(𝐶𝐷!!!!")isexpressedinmdeg,Θisthemolarellipticity,and𝑙isthepathlengthincm.CoupledkinaseassaysKinaseactivitywasmonitoredwithpreviouslydescribedconditions(Barkeretal.1995;Chaoetal.2010)usingaSynergyH1microplatereader(Biotek).Theadditionofcalmodulin(concentrationsrangingfrom0nM-2µM)tothereactionwasusedtoinitiateCaMKIIactivity,afterwhichabsorbancewasmeasuredat15secondintervalsfor10minutes.Thechangeinabsorbanceoverthecourseoftheresultingtimeseriesofmeasurementswasfitwithastraightline(y=mx+c)toobtainaslope(m)proportionaltothekineticrateofthereaction.Foreachtimeseries,slopeswerefittoaslidingwindowof5points(1minute15seconds)andthemaximumobservedslopewasusedtorepresentthekineticrateofthatreaction.Kineticratesacrossaseriesofcalmodulinconcentrationswerefitwiththefollowingequation:
𝑌 = 𝑌!"# + 𝑌!"# − 𝑌!"# ∙ 𝑋!!𝑋!! + 𝐸𝐶!"!!
toobtainEC50(definedasthecalmodulinconcentrationneededtoreachhalfthemaximalreactionvelocity)andcooperativityvalues(Hillcoefficients,nH).95%confidenceintervalsforfitparameters(EC50andnH)weredeterminedusingthefollowingbootstrapprocedure.10,000
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replicatecalmodulinconcentrationseriesweregeneratedbyrandomlyselectingoneobservedkineticrateateachmeasuredcalmodulinconcentrationfromthesetofreplicatesforthatvariant.Eachgeneratedconcentrationserieswasfitwiththeequationabove.Parametervaluesatthe2.5thand97.5thquantilesofthe10,000fitsweretakenastheboundariesofthe95%confidenceinterval.AcknowledgementsWethankLukeChao,FlorianHeydandAndreasFranzforhelpfuldiscussionsondataandthemanuscript.WealsothankPeterChien,EricStrieter,ScottGarmanforhelpfuldiscussions.REFERENCES1. Lin,Y.C.,andRedmond,L.(2009)NeuronalCaMKIIactsasastructuralkinase.
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certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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20. Ma,H.,Groth,R.D.,Cohen,S.M.,Emery,J.F.,Li,B.,Hoedt,E.,Zhang,G.,Neubert,T.A.,andTsien,R.W.(2014)gammaCaMKIIshuttlesCa(2)(+)/CaMtothenucleustotriggerCREBphosphorylationandgeneexpression.Cell159,281-294
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24. O'Leary,H.,Lasda,E.,andBayer,K.U.(2006)CaMKIIbetaassociationwiththeactincytoskeletonisregulatedbyalternativesplicing.MolBiolCell17,4656-4665
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certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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26. Okamoto,K.,Narayanan,R.,Lee,S.H.,Murata,K.,andHayashi,Y.(2007)TheroleofCaMKIIasanF-actin-bundlingproteincrucialformaintenanceofdendriticspinestructure.ProcNatlAcadSciUSA104,6418-6423
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34. Chang,H.Y.,Minahan,K.,Merriman,J.A.,andJones,K.T.(2009)Calmodulin-dependentproteinkinasegamma3(CamKIIgamma3)mediatesthecellcycleresumptionofmetaphaseIIeggsinmouse.Development136,4077-4081
35. Mukherjee,S.,Sheng,W.,Sun,R.,andJanssen,L.J.(2017)Ca(2+)/calmodulin-dependentproteinkinaseIIbetaandIIdeltamediateTGFbeta-inducedtransductionoffibronectinandcollageninhumanpulmonaryfibroblasts.AmJPhysiolLungCellMolPhysiol312,L510-L519
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FOOTNOTES Funding was provided by the University of Massachusetts, Amherst and a grant to M.M.S. from the National Institute of General Medical Sciences (R01GM123157).
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MAINFIGURES
Figure1.CaMKIIsequencedivergenceandstructuralorganization.(A)EachsubunitofCaMKIIconsistsofakinasedomain,regulatorysegment,variablelinkerregion,andhubdomain.Theregulatorysegmenthousesthecalmodulinbindingregionaswellasaregulatoryautophosphorylationsite,Thr286.(B)Theregulatorysegmentactsasanautoinhibitorydomainbyblockingthesubstrate-bindingpocket.InthepresenceofCa2+,Ca2+-CaMbindstheregulatorysegment,exposingthesubstrate-bindingpocketandturningtheenzymeon.(C)ThehubdomainoligomerizesCaMKIIintotwostackedhexamericrings.ShownisacartoonofanactivestructurewithCa2+-CaMboundtotheregulatorysegment.(D)NeighborJoiningreconstructionsofkinaseandhubdomaindivergenceforthefourhumanCaMKIIgenes,rootedwithD.melanogasterCaMKIIoutgroups.
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Figure2.IlluminasequencingrevealsaheterogeneouspopulationofCaMKIIvariantsinthehumanhippocampus.(A)HumanCaMKIIgenesareencodedby24exonswithclearone-to-onecorrespondenceacrossgenes.Exons1-10encodethecatalytickinasedomain,11and12encodetheregulatorysegment,13-21encodethevariable-lengthlinker,and22-24encodetheassociation(hub)domain.Missinglinkerexonsindicatethattheexonisnotencodedinthecorrespondinggene.Constitutivelyincorporatedkinase,regulatorysegment,andhubexons(grey)aresplicedintoeverytranscript.CaMKIIδencodestwoversionsofkinaseexon6,withoneortheother,butnotboth,incorporatedintotranscripts.Severalexonscontainalternativesplicesites,permittingomissionofafractionoftheexon(indicatedbyhatching).CaMKIIβ
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encodesthreenearlyidenticalproline-richlinkerexons:19-21,homologoustoCaMKIIγexon19.(B)IlluminalibraryconstructionisillustratedwithCaMKIIβ.InitialPCRamplificationofaregionencompassingallalternativesplicesites(includingthelinkerandhubexon23)iscarriedoutwithprimersdesignedforspecificityfortargetCaMKIIgene,totheexclusionoftheotherthreegenes(orangeandredarrows).InitialampliconsserveastemplatesforamplificationoflibraryinsertsinasecondPCRreactionwithadifferentprimerpair(greenandbluearrows).Resultinginsertsarefullyspannedbypaired-end251bpreadsfornearlyallsplicevariants.ForCaMKIIβ,thisistrueaslongasexons19,20,and21arenotincorporated.Theseexonsareshownassemi-transparentbecausetheywerenotincorporatedinanydetectedhippocampalsplicevariant.(C)SummaryofCaMKIIαandβtranscriptvariantsdetectedbyIlluminasequencing.Alldetectedvariants,includingallpreviouslyannotatedvariants,areshownforCaMKIIα.ForCaMKIIβ,thetopeightread-mappingvariantsareshownabovetheblacklineandtheremainingpreviouslyannotatedvariantsareshownbelowtheline.Variantsareconsiderednoveliftheyhavenotbeenpreviouslyannotated.Variantshighlightedinbluearethosethatwereusedinactivitymeasurements(seeFigure3).
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Figure3.ThehubdomainplaysacrucialroleinCa2+/CaMsensitivity.CaMKIIactivityagainstapeptidesubstrate(syntide)wasmeasuredasafunctionofCa2+-CaMconcentration.Tosimplifycomparisons,fitcurveswerenormalizedbythecorrespondingVmaxfitparameteranddatapointsarenotshown(seeSupplement3).TheEC50valueforeachfitisindicatedbyasinglebox,anddashedgraylinesinB-DindicatereferenceEC50valuesforCaMKIIα-30andCaMKIIα-0(B)orCaMKIIβ-0andCaMKIIα-0(C,D),aslabeledontheX-axis.ActivitymeasurementsandEC50andnH(Hillcoefficient)fitparametersareshownforallthreeCaMKIIαsplicevariants(A),eightselectedCaMKIIβvariants(B),chimerasofCaMKIIα/βwithnolinker(C),andCaMKIIγandδ
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withnolinker(D).EC50andHillcoefficient(nH)fitparameterswith95%confidenceintervals(seeMaterialsandMethods)arelistedforeachtestedvariant.CartoonrepresentationsofCaMKIIholoenzymesintheirautoinhibitedstatesareshowninpanelA.ForpanelsCandD,eachdomainislabeledaccordingtotheCaMKIIgene.
Figure4.AllostericcontributionsfromthehubdomainregulateCaMKIIactivation.(A)The32positionsthatdifferbetweenCaMKIIαandβhubsarerepresentedbyspheresatthealphaCarbonpositiononthestructureofCaMKIIαhub(3SOA).Hubisshownincyan,regulatorysegmentinorange,andkinasedomainingreen.(B)SequencealignmentofCaMKIIα,β,γ,andδhubdomains,coloredbydegreeofconservation(darktolight=mosttoleastconserved).Forconvenience,allhubsequencesstartatresidue1.ResiduesinCaMKIIαhubthatdifferinCaMKIIβarehighlightedbyredboxes.
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SUPPLEMENTALINFORMATIONSupplement1:IlluminasequencingresultsforallCaMKIIgenes.
Variantname Ensembl identifierCCDS
identifier
NCBI
NameNCBI identifier
Read
count
%of
reads
CaMKIIαα14,18 ENST00000348628 CCDS43386 var.2 NM_171825 183118 94.6
α-no-linker novel 7527 3.89
α14,15,18 ENST00000398376 CCDS43387 var.1 NM_015981 2941 1.52
Variantname Ensembl identifier CCDSidentifier
NCBIName NCBI identifier Read
count%ofreads
CaMKIIββ13,14b,16,17,18 ENST00000350811 CCDS5484 var.2 NM_172078 65577 44.8
β14a,16,18 ENST00000358707 CCDS5486 var.5 NM_172081 20153 13.8
β14a,16,17,18 ENST00000395747 CCDS5485 var.3 NM_172079 13327 9.11
β14b,16,17,18 ENST00000258682 CCDS43573 var.4 NM_172080 10362 7.08
β14b,16,18 novel var.X20 XM_017012664 9192 6.28
β13,14b,16,18 novel var.X24 XM_017012667 8616 5.89
β13,14b,17,18 novel var.X26 XM_017012669 5805 3.97
β13,14b,16,17,18-shorthub novel 5442 3.72
β14b,17,18 novel var.X22 XM_017012665 1700 1.16
β14a,17,18 novel var.X21 XM_005249864 1218 0.83
β13,14b,18 novel 928 0.63
β14a,18 ENST00000353625 CCDS5488 var.7 NM_172083 750 0.51
β14b,16,17,18-shorthub novel 634 0.43
β13,14a,16,17,18 novel 412 0.28
β13,14b,16,18-shorthub novel 400 0.27
β14b,18 novel 352 0.24
β14a,16,17,18-shorthub novel 309 0.21
β14a,16,18-short hub novel 303 0.21
β-no-linker ENST00000346990 CCDS5489 var.8 NM_172084 185 0.13
β13,14a,16,18 novel 169 0.12
β14a,17,18-short hub novel 155 0.11
β13,14b,17,18-shorthub ENST00000347193 CCDS5487 var.6 NM_172082 148 0.10
β14b,16,18-short hub novel 104 0.071
β13 novel 52 0.036
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Variantname Ensembl identifierCCDS
identifier
NCBI
NameNCBI identifier
Read
count
%of
reads
CaMKIIδδ61,14b,18 ENST00000514328 CCDS82950 var.15 NM_001321574 45214 53.0
δ61,14a,18
ENST00000342666
ENST00000379773
ENST00000394524
CCDS3703
CCDS3704
CCDS43263
var.3
var.4
var. 2
NM_001221
NM_172127
NM_172128
24712 29.0
δ61,14a,16,17,18 novel 6483 7.64
δ62,14b,18 novel 4001 4.71
δ62,14a,18 novel 1225 1.44
δ61,14a,17,18 ENST00000394522 CCDS47127 var.6 NM_172114 1121 1.32
δ61,14b,17,18 novel 820 0.97
δ62,14a,17,18 novel 563 0.66
δ61,14b novel 431 0.51
δ61,14a novel 326 0.38
δ61,14b,16,17,18 novel 84 0.10
δ61,14a,15,17,18 novel 90 0.11
VariantnameEnsembl
identifier
CCDS
identifier
NCBI
NameNCBI identifier
Read
count
%of
reads
CaMKIIγγ14,16a,17,18b,19a ENST00000322680 CCDS7338 var.1 NM_172171 53510 31.3
γ14,16a,17,18b ENST00000322635 CCDS7337 var.2 NM_172169 47800 28.0
γ14,15,17,18b var.12 NM_001367519 19801 11.6
γ14,17,18b,19a var.21 NM_001367528 14773 8.65
γ14,15,17,18b,19a var.11 NM_001367518 12127 7.09
γ14,17,18b ENST00000351293 CCDS7336 var.4 NM_001222 10268 6.01
γ14,16b,17,18b,19a var.13 NM_001367520 4586 2.68
γ14,16b,17,18b var.6 NM_172173 3253 1.90
γ14,15,16a,17,18b ENST00000305762 var.24 NM_001367531 2196 1.29
γ14,15,16a,17,18b,19a var.40 NM_001367547 1662 0.97
γ14,18b,19a novel 160 0.094
γ14,15,18b novel 133 0.078
γ14,15 novel,premature termination 111 0.065
γ14,15,16b,17,18b novel var.X32 XM_017016730 100 0.059
γ14,17,18a,19b novel 96 0.056
γ14,18b novel 81 0.047
γ14,15,16b,17,18b,19a novel 72 0.042
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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Supplement2:ClusterconsensussequencesforallCaMKIIgenes.CaMKIIalpha_proteinsequences>alpha14,18WISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCD>alpha-no-linkerWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTVMLATRNFSVRKQEIIKVTEQLIEAISNGDFESYTKMCD>alpha14,15,18WISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKSDGVKKRKSSSSVQLMESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDCaMKIIalpha_DNAsequences>alpha14,18TGGATCTCGCACCGCTCCACCGTGGCATCCTGCATGCACAGACAGGAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAGGGAGCCATTCTCACCACGATGCTGGCCACCAGGAACTTCTCCGGAGGGAAGAGTGGGGGAAACAAGAAGAGCGATGGTGTGAAGGAATCCTCAGAGAGCACCAACACCACCATCGAGGATGAAGACACCAAAGTGCGGAAACAGGAAATTATAAAAGTGACAGAGCAGCTGATTGAAGCCATAAGCAATGGAGATTTTGAGTCCTACACGAAGATGTGCGAC>alpha-no-linkerTGGATCTCGCACCGCTCCACCGTGGCATCCTGCATGCACAGACAGGAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAGGGAGCCATTCTCACCGTGATGCTGGCCACCAGGAACTTCTCCGTGCGGAAACAGGAAATTATAAAAGTGACAGAGCAGCTGATTGAAGCCATAAGCAATGGAGATTTTGAGTCCTACACGAAGATGTGCGAC>alpha14,15,18TGGATCTCGCACCGCTCCACCGTGGCATCCTGCATGCACAGACAGGAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAGGGAGCCATTCTCACCACGATGCTGGCCACCAGGAACTTCTCCGGAGGGAAGAGTGGGGGAAACAAGAAGAGCGATGGTGTGAAGAAAAGAAAGTCCAGTTCCAGCGTTCAGTTAATGGAATCCTCAGAGAGCACCAACACCACCATCGAGGATGAAGACACCAAAGTGCGGAAACAGGAAATTATAAAAGTGACAGAGCAGCTGATTGAAGCCATAAGCAATGGAGATTTTGAGTCCTACACGAAGATGTGCGAC
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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CaMKIIbeta_proteinsequences>beta13,14b,16,17,18ILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIHNPVDGIKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta14a,16,18ILTTMLATRNFSAAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta14a,16,17,18ILTTMLATRNFSAAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIHNPVDGIKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta14b,16,17,18ILTTMLATRNFSAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIHNPVDGIKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta14b,16,18ILTTMLATRNFSAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta13,14b,16,18ILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta13-14b-17-18ILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKEPQTTVIHNPVDGIKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta13,14b,16,17,18-short-hubILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIHNPVDGIKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAFYFENLL>beta14b,17,18ILTTMLATRNFSAKSLLNKKADGVKEPQTTVIHNPVDGIKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta14a-17-18ILTTMLATRNFSAAKSLLNKKADGVKEPQTTVIHNPVDGIKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta13,14b,18
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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ILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta14a,18ILTTMLATRNFSAANSLLNKKADGVKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta14b,16,17,18ILTTMLATRNFSAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIHNPVDGIKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAFYFENLL>beta13,14b,16,18ILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAFYFENLL>beta14b,18ILTTMLATRNFSAKSLLNKKADGVKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta14a,16,17,18ILTTMLATRNFSAANSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIHNPVDGIKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAFYFENLL>beta14a,16,18ILTTMLATRNFSAAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAFYFENLL>beta13,14a,16,17,18ILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIHNPVDGIKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta-no-linkerILTTMLATRNFSARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta13,14a,16,18ILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAAKSLLNKKADGVKPQTNSTNNSAAATSPKGTLPPAALESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLL>beta14a,17,18-short-hubILTTMLATRNFSAAKSLLNKKADGVKEPQTTVIHNPVDGIKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAFYFENLL>beta13,14b,17,18-short-hub
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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ILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKEPQTTVIHNPVDGIKESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAFYFENLL>beta14b,16,18-short-hubILTTMLATRNFSAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAFYFENLL>beta13ILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLCaMKIIbeta_DNAsequences>beta13,14b,16,17,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGTGGGCAGACAGACCACCGCTCCGGCCACAATGTCCACCGCGGCCTCCGGCACCACCATGGGGCTGGTGGAACAAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGCCTCAAACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta14a,16,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta14a,16,17,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGCCTCAAACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGC
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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TAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta14b,16,17,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGCCTCAAACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta14b,16,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta13,14b,16,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGTGGGCAGACAGACCACCGCTCCGGCCACAATGTCCACCGCGGCCTCCGGCACCACCATGGGGCTGGTGGAACAAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta13-14b-17-18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGTGGGCAGACAGACCACCGCTCCGGCCACAATGTCCACCGCGGCCTCCGGCACCACCATGGGGCTGGTGGAACAAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGGAGCCTCAA
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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ACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta13,14b,16,17,18-short-hubCATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGTGGGCAGACAGACCACCGCTCCGGCCACAATGTCCACCGCGGCCTCCGGCACCACCATGGGGCTGGTGGAACAAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGCCTCAAACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCATTCTACTTCGAGAACCTGCTG>beta14b,17,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGGAGCCTCAAACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta14a-17-18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGGAGCCTCAAACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta13,14b,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGTGGGCAGACAGACCACCGCTCCGGCCACAATGTCCACCGCGGCCTCCGGCACCACCATGGGGCTGGTGGAACAAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGGAGTCTTCT
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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GACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta14a,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCAGCCAATAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta14b,16,17,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGCCTCAAACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCATTCTACTTCGAGAACCTGCTG>beta13,14b,16,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGTGGGCAGACAGACCACCGCTCCGGCCACAATGTCCACCGCGGCCTCCGGCACCACCATGGGGCTGGTGGAACAAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCATTCTACTTCGAGAACCTGCTG>beta14b,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta14a,16,17,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCAGCCAATAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGC
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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CCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGCCTCAAACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCATTCTACTTCGAGAACCTGCTG>beta14a,16,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCATTCTACTTCGAGAACCTGCTG>beta13,14a,16,17,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGTGGGCAGACAGACCACCGCTCCGGCCACAATGTCCACCGCGGCCTCCGGCACCACCATGGGGCTGGTGGAACAAGCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGCCTCAAACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta-no-linkerCATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta13,14a,16,18CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGTGGGCAGACAGACCACCGCTCCGGCCACAATGTCCACCGCGGCCTCCGGCACCACCATGGGGCTGGTGGAACAAGCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAACAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGG
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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CCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTG>beta14a,17,18-short-hubCATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGGAGCCTCAAACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCATTCTACTTCGAGAACCTGCTG>beta13,14b,17,18-short-hubCATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGTGGGCAGACAGACCACCGCTCCGGCCACAATGTCCACCGCGGCCTCCGGCACCACCATGGGGCTGGTGGAACAAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGGAGCCTCAAACCACCGTCATCCATAACCCAGTGGACGGGATTAAGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCATTCTACTTCGAGAACCTGCTG>beta14b,16,18-short-hubCATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGTCAAGCCCCAGACGAATAGCACCAAAAACAGTGCAGCCGCCACCAGCCCCAAAGGGACGCTTCCTCCTGCCGCCCTGGAGTCTTCTGACAGTGCCAATACCACCATAGAGGATGAAGACGCTAAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCATTCTACTTCGAGAACCTGCTG>beta13CATCCTCACCACCATGCTGGCCACACGGAATTTCTCAGTGGGCAGACAGACCACCGCTCCGGCCACAATGTCCACCGCGGCCTCCGGCACCACCATGGGGCTGGTGGAACAAGCCCGGAAGCAGGAGATCATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTGACTTTGAGGCCTACGCGAAAATCTGTGACCCAGGGCTGACCTCGTTTGAGCCTGAAGCACTGGGCAACCTGGTTGAAGGGATGGACTTCCACAGATTCTACTTCGAGAACCTGCTGCaMKIIdelta_proteinsequences>Fread,delta6v1,14b-18SEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPY>Rread,delta6v1,14b-18
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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ICQRSTVASMMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTE>Fread,delta6v1,14a-18SEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPY>Rread,delta6v1,14a-18CQRSTVASMMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTE>Fread,delta6v1,14a-16-17-18SEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPY>Rread,delta6v1,14a-16-17-18TMLATRNFSAAKSLLKKPDGVKINNKANVVTSPKENIPTPALEPQTTVIHNPDGNKESTESSNTTIEDEDVKARKQEIIKVTE>Fread,delta6v2,14b-18SEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPY>Rread,delta6v2,14b-18ICQRSTVASMMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTE>Fread,delta6v2,14a-18SEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLLKDPY>Rread,delta6v2,14a-18CQRSTVASMMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTE>Fread,delta6v1,14a-17-18SEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPY>Rread,delta6v1,14a-17-18TVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKEPQTTVIHNPDGNKESTESSNTTIEDEDVKARKQEIIKVTE>Fread,delta6v1,14b-17-18
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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30
SEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPY>Rread,delta6v1,14b-17-18ETVDCLKKFNARRKLKGAILTTMLATRNFSAKSLLKKPDGVKEPQTTVIHNPDGNKESTESSNTTIEDEDVKARKQEIIKVTE>Fread,delta6v2,14a-17-18SEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPY>Rread,delta6v2,14a-17-18TVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKEPQTTVIHNPDGNKESTESSNTTIEDEDVKARKQEIIKVTE>Fread,delta6v1,14bSEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPY>Rread,delta6v1,14bQPCQTHHSLRGTEAPMDLSTFYCCFHDAQTGDCRLLEEI*C*KKTKGCHLDNYAGYKEFLSQEFVEETRWSKARKQEIIKVTE>Fread,delta6v1,14aSEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPY>Rread,delta6v1,14aPCQTHHSLRGTEAPMDLSTFYCCFHDAQTGDCRLLEEI*C*KKTKGCHLDNYAGYKEFLSSQEFVEETRWSKARKQEIIKVTE>Fread,delta6v1,14b-16-17-18SEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPY>Rread,delta6v1,14b-16-17-18TTMLATRNFSAKSLLKKPDGVKINNKANVVTSPKENIPTPALEPQTTVIHNPDGNKESTESSNTTIEDEDVKARKQEIIKVTE>Fread,delta6v1,14a-15-17-18SEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPY>Rread,delta6v1,14a-15-17-18
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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31
RKLKGAILTTMLATRNFSAAKSLLKKPDGVKKRKSSSSVQMMEPQTTVIHNPDGNKESTESSNTTIEDEDVKARKQEIIKVTECaMKIIdelta_DNAsequences>Fread,delta6v1,14b-18CAGTGAAGCTGATGCCAGTCATTGTATACAGCAGATTCTAGAAAGTGTTAATCATTGTCACCTAAATGGCATAGTTCACAGGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACGTAAAGATCCTTATG>Rread,delta6v1,14b-18GATCTGTCAACGTTCTACTGTTGCTTCCATGATGCACAGACAGGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGAAGAAAACTAAAGGGTGCCATCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGTAAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGGATGAAGATGTGAAAGCACGAAAGCAAGAGATTATCAAAGTCACTGAAC>Fread,delta6v1,14a-18CAGTGAAGCTGATGCCAGTCATTGTATACAGCAGATTCTAGAAAGTGTTAATCATTGTCACCTAAATGGCATAGTTCACAGGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACGTAAAGATCCTTATG>Rread,delta6v1,14a-18CTGTCAACGTTCTACTGTTGCTTCCATGATGCACAGACAGGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGAAGAAAACTAAAGGGTGCCATCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGTAAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGGATGAAGATGTGAAAGCACGAAAGCAAGAGATTATCAAAGTCACTGAAC>Fread,delta6v1,14a-16-17-18CAGTGAAGCTGATGCCAGTCATTGTATACAGCAGATTCTAGAAAGTGTTAATCATTGTCACCTAAATGGCATAGTTCACAGGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACGTAA
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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AGATCCTTATG>Rread,delta6v1,14a-16-17-18AACTATGCTGGCTACAAGGAATTTCTCAGCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGTAAAGATAAACAACAAAGCCAACGTGGTAACCAGCCCCAAAGAAAATATTCCTACCCCAGCGCTGGAGCCCCAAACTACTGTAATCCACAACCCTGATGGAAACAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGGATGAAGATGTGAAAGCACGAAAGCAAGAGATTATCAAAGTCACTGAAC>Fread,delta6v2,14b-18CAGTGAAGCTGATGCCAGTCATTGCATTCAGCAGATCCTGGAGGCTGTGCTACACTGCCATCAGATGGGCGTGGTCCATCGGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACGTAAAGATCCTTATG>Rread,delta6v2,14b-18GATCTGTCAACGTTCTACTGTTGCTTCCATGATGCACAGACAGGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGAAGAAAACTAAAGGGTGCCATCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGTAAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGGATGAAGATGTGAAAGCACGAAAGCAAGAGATTATCAAAGTCACTGAAC>Fread,delta6v2,14a-18CAGTGAAGCTGATGCCAGTCATTGCATTCAGCAGATCCTGGAGGCTGTGCTACACTGCCATCAGATGGGCGTGGTCCATCGGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACTTAAAGATCCTTATG>Rread,delta6v2,14a-18CTGTCAACGTTCTACTGTTGCTTCCATGATGCACAGACAGGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGAAGAAAACTAAAGGGTGCCATCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGTAAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGGATGAAGATGTGAAAGCACGAAAGCAAGAGATTATCAAAGTCACTGAAC>Fread,delta6v1,14a-17-18CAGTGAAGCTGATGCCAGTCATTGTATACAGCAGATTCTAGAAAGTGTTAATCATTGTCACCTAAATGGCATAGTTCACA
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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GGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACGTAAAGATCCTTATG>Rread,delta6v1,14a-17-18GACTGTAGACTGCTTGAAGAAATTTAATGCTAGAAGAAAACTAAAGGGTGCCATCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGTAAAGGAGCCCCAAACTACTGTAATCCACAACCCTGATGGAAACAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGGATGAAGATGTGAAAGCACGAAAGCAAGAGATTATCAAAGTCACTGAAC>Fread,delta6v1,14b-17-18CAGTGAAGCTGATGCCAGTCATTGTATACAGCAGATTCTAGAAAGTGTTAATCATTGTCACCTAAATGGCATAGTTCACAGGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACGTAAAGATCCTTATG>Rread,delta6v1,14b-17-18GGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGAAGAAAACTAAAGGGTGCCATCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGTAAAGGAGCCCCAAACTACTGTAATCCACAACCCTGATGGAAACAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGGATGAAGATGTGAAAGCACGAAAGCAAGAGATTATCAAAGTCACTGAAC>Fread,delta6v2,14a-17-18CAGTGAAGCTGATGCCAGTCATTGCATTCAGCAGATCCTGGAGGCTGTGCTACACTGCCATCAGATGGGCGTGGTCCATCGGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACGTAAAGATCCTTATG>Rread,delta6v2,14a-17-18GACTGTAGACTGCTTGAAGAAATTTAATGCTAGAAGAAAACTAAAGGGTGCCATCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGTAAAGGAGCCCCAAACTACTGTAATCCACAACCCTGATGGAAACAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGGATGAAGATGTGAAAGCACGAAAGCAAGAGATTATCAA
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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AGTCACTGAAC>Fread,delta6v1,14bCAGTGAAGCTGATGCCAGTCATTGTATACAGCAGATTCTAGAAAGTGTTAATCATTGTCACCTAAATGGCATAGTTCACAGGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACGTAAAGATCCTTATG>Rread,delta6v1,14bTCAACCCTGCCAAACGCATCACAGCCTCAGAGGCACTGAAGCACCCATGGATCTGTCAACGTTCTACTGTTGCTTCCATGATGCACAGACAGGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGAAGAAAACTAAAGGGTGCCATCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGTAAAGCACGAAAGCAAGAGATTATCAAAGTCACTGAAC>Fread,delta6v1,14aCAGTGAAGCTGATGCCAGTCATTGTATACAGCAGATTCTAGAAAGTGTTAATCATTGTCACCTAAATGGCATAGTTCACAGGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACGTAAAGATCCTTATG>Rread,delta6v1,14aACCCTGCCAAACGCATCACAGCCTCAGAGGCACTGAAGCACCCATGGATCTGTCAACGTTCTACTGTTGCTTCCATGATGCACAGACAGGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGAAGAAAACTAAAGGGTGCCATCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGTAAAGCACGAAAGCAAGAGATTATCAAAGTCACTGAAC>Fread,delta6v1,14b-16-17-18CAGTGAAGCTGATGCCAGTCATTGTATACAGCAGATTCTAGAAAGTGTTAATCATTGTCACCTAAATGGCATAGTTCACAGGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACGTAAAGATCCTTATG>Rread,delta6v1,14b-16-17-18GACAACTATGCTGGCTACAAGGAATTTCTCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGTAAAGATAAACAACAAAG
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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CCAACGTGGTAACCAGCCCCAAAGAAAATATTCCTACCCCAGCGCTGGAGCCCCAAACTACTGTAATCCACAACCCTGATGGAAACAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGGATGAAGATGTGAAAGCACGAAAGCAAGAGATTATCAAAGTCACTGAAC>Fread,delta6v1,14a-15-17-18CAGTGAAGCTGATGCCAGTCATTGTATACAGCAGATTCTAGAAAGTGTTAATCATTGTCACCTAAATGGCATAGTTCACAGGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACGTAAAGATCCTTATG>Rread,delta6v1,14a-15-17-18AAGAAAACTAAAGGGTGCCATCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGTAAAGAAAAGGAAGTCCAGTTCGAGTGTTCAGATGATGGAGCCCCAAACTACTGTAATCCACAACCCTGATGGAAACAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGGATGAAGATGTGAAAGCACGAAAGCAAGAGATTATCAAAGTCACTGAACCaMKIIgamma_proteinsequences>gamma14,16a,17,18b,19aLTTMLVSRNFSAAKSLLNKKSDGGVKPQSNNKNSLVSPAQEPAPLQTAMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKAAPLRTGNGSSVPEGRSSRDRTAPSAGMQPQPSLCSSAMRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,16a,17,18bLTTMLVSRNFSAAKSLLNKKSDGGVKPQSNNKNSLVSPAQEPAPLQTAMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,15,17,18bLTTMLVSRNFSAAKSLLNKKSDGGVKKRKSSSSVHLMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,17,18b,19aLTTMLVSRNFSAAKSLLNKKSDGGVKEPQTTVVHNATDGIKGSTESCNTTTEDEDLKAAPLRTGNGSSVPEGRSSRDRTAPSAGMQPQPSLCSSAMRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,15,17,18b,19aLTTMLVSRNFSAAKSLLNKKSDGGVKKRKSSSSVHLMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKAAPLRTGNGSSV
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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PEGRSSRDRTAPSAGMQPQPSLCSSAMRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,17,18bLTTMLVSRNFSAAKSLLNKKSDGGVKEPQTTVVHNATDGIKGSTESCNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,16b,17,18b,19aLTTMLVSRNFSAAKSLLNKKSDGGVKPQSNNKNSLEPQTTVVHNATDGIKGSTESCNTTTEDEDLKAAPLRTGNGSSVPEGRSSRDRTAPSAGMQPQPSLCSSAMRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,16b,17,18bLTTMLVSRNFSAAKSLLNKKSDGGVKPQSNNKNSLEPQTTVVHNATDGIKGSTESCNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,15,16a,17,18bLTTMLVSRNFSAAKSLLNKKSDGGVKKRKSSSSVHLMPQSNNKNSLVSPAQEPAPLQTAMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,15,16a,17,18b,19aLTTMLVSRNFSAAKSLLNKKSDGGVKKRKSSSSVHLMPQSNNKNSLVSPAQEPAPLQTAMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKAAPLRTGNGSSVPEGRSSRDRTAPSAGMQPQPSLCSSAMRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,18b,19aLTTMLVSRNFSAAKSLLNKKSDGGVKGSTESCNTTTEDEDLKAAPLRTGNGSSVPEGRSSRDRTAPSAGMQPQPSLCSSAMRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,15,18bLTTMLVSRNFSAAKSLLNKKSDGGVKKRKSSSSVHLMGSTESCNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,15LTTMLVSRNFSAAKSLLNKKSDGGVKKRKSSSSVHLMCENRRSLRLQNS*LKPSTMGTLRPTRRFVIQASLPLSLRPLVTSWR>gamma14,15,16b,17,18bLTTMLVSRNFSAAKSLLNKKSDGGVKKRKSSSSVHLMPQSNNKNSLEPQTTVVHNATDGIKGSTESCNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,17,18a,19bLTTMLVSRNFSAAKSLLNKKSDGGVKEPQTTVVHNATDGIKGSTESCNTTTEDEDLKARCLKDGAPGTEQPPLQACSPSLLSAPQPCENRRSLRLQNS*LKPSTMGTLRPTRRFVIQASLPLSLRPLVTSWR>gamma14,18b
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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LTTMLVSRNFSAAKSLLNKKSDGGVKGSTESCNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVE>gamma14,15,16b,17,18b,19aLTTMLVSRNFSAAKSLLNKKSDGGVKKRKSSSSVHLMPQSNNKNSLEPQTTVVHNATDGIKGSTESCNTTTEDEDLKAAPLRTGNGSSVPEGRSSRDRTAPSAGMQPQPSLCSSAMRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVECaMKIIgamma_DNAsequences>gamma14,16a,17,18b,19aCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGCCACAGAGCAACAACAAAAACAGTCTCGTAAGCCCAGCCCAAGAGCCCGCGCCCTTGCAGACGGCCATGGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGCTGCCCCGCTCCGCACTGGGAATGGCAGCTCGGTGCCTGAAGGACGGAGCTCCCGGGACAGAACAGCCCCCTCTGCAGGCATGCAGCCCCAGCCTTCTCTCTGCTCCTCAGCCATGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,16a,17,18bCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGCCACAGAGCAACAACAAAAACAGTCTCGTAAGCCCAGCCCAAGAGCCCGCGCCCTTGCAGACGGCCATGGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGTGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,15,17,18bCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGAAAAGGAAGTCGAGTTCCAGCGTGCACCTAATGGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGTGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTG
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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AGGCCCTTGGTAACCTCGTGGAGGG>gamma14,17,18b,19aCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGCTGCCCCGCTCCGCACTGGGAATGGCAGCTCGGTGCCTGAAGGACGGAGCTCCCGGGACAGAACAGCCCCCTCTGCAGGCATGCAGCCCCAGCCTTCTCTCTGCTCCTCAGCCATGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,15,17,18b,19aCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGAAAAGGAAGTCGAGTTCCAGCGTGCACCTAATGGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGCTGCCCCGCTCCGCACTGGGAATGGCAGCTCGGTGCCTGAAGGACGGAGCTCCCGGGACAGAACAGCCCCCTCTGCAGGCATGCAGCCCCAGCCTTCTCTCTGCTCCTCAGCCATGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,17,18bCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGTGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,16b,17,18b,19aCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGCCACAGAGCAACAACAAAAACAGTCTCGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGCTGCCCCGCTCCGCACTGGGAATGGCAGCTCGGTGCCTGAAGGACGGAGCTCCCGGGACAGAACAGCCCCCTCTGCAGGCATGCAGCCCCAGCCTTCTCTCTGCTCCTCAGCCATGCGAA
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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AACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,16b,17,18bCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGCCACAGAGCAACAACAAAAACAGTCTCGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGTGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,15,16a,17,18bCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGAAAAGGAAGTCGAGTTCCAGCGTGCACCTAATGCCACAGAGCAACAACAAAAACAGTCTCGTAAGCCCAGCCCAAGAGCCCGCGCCCTTGCAGACGGCCATGGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGTGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,15,16a,17,18b,19aCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGAAAAGGAAGTCGAGTTCCAGCGTGCACCTAATGCCACAGAGCAACAACAAAAACAGTCTCGTAAGCCCAGCCCAAGAGCCCGCGCCCTTGCAGACGGCCATGGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGCTGCCCCGCTCCGCACTGGGAATGGCAGCTCGGTGCCTGAAGGACGGAGCTCCCGGGACAGAACAGCCCCCTCTGCAGGCATGCAGCCCCAGCCTTCTCTCTGCTCCTCAGCCATGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,18b,19aCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGCTGCCCCGCTCCGCACTGGGAATGGCAGCTCG
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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GTGCCTGAAGGACGGAGCTCCCGGGACAGAACAGCCCCCTCTGCAGGCATGCAGCCCCAGCCTTCTCTCTGCTCCTCAGCCATGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,15,18bCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGAAAAGGAAGTCGAGTTCCAGCGTGCACCTAATGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGTGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,15CCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGAAAAGGAAGTCGAGTTCCAGCGTGCACCTAATGTGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,15,16b,17,18bCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGAAAAGGAAGTCGAGTTCCAGCGTGCACCTAATGCCACAGAGCAACAACAAAAACAGTCTCGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGTGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,17,18a,19bCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGCTCGGTGCCTGAAGGACGGAGCTCCCGGGACAGAACAGCCCCCTCTGCAGGCATGCAGCCCCAGCCTTCTCTCTGCTCCTCAGCCATGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,18b
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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CCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGTGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG>gamma14,15,16b,17,18b,19aCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGAAAAGGAAGTCGAGTTCCAGCGTGCACCTAATGCCACAGAGCAACAACAAAAACAGTCTCGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCACAGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGCTGCCCCGCTCCGCACTGGGAATGGCAGCTCGGTGCCTGAAGGACGGAGCTCCCGGGACAGAACAGCCCCCTCTGCAGGCATGCAGCCCCAGCCTTCTCTCTGCTCCTCAGCCATGCGAAAACAGGAGATCATTAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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Supplement3:CaMKIIactivitymeasurementswithalldatapointsshown.Individualdatasetsarecolor-coded.
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint
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Supplement4:PCRampliconsfrommultipleCaMKIIβ variantsdonotseparateonagarosegel.APCRreactionwithCaMKIIβ-specificprimerswasloadedontoa2.5%agarosegelandrunat10Vforroughly24hrs(picturedbelow)foroptimalbandseparation.Thesinglevisiblebandwasexcised,purified,andSanger-sequenced.Afragmentoftheresultingsequencingtraceisshown,withheightofgreybackgroundrepresentingPhredqualityscores.Thefirst15bpofthefragment(leftofgreyline)maptothelast15bpofCaMKIIβconstitutiveexon12,incorporatedintoeveryCaMKIIβtranscript.Thelast15bpofthefragment(rightofgreyline)showadramaticincreaseofsecondarypeakheights,withacorrespondingdrop-offinPhredscores.Thesequencedefinedbyprimarypeaks(TGGGCAGACAGACCA)mapstoCaMKIIβlinkerexon13.Variantsβ13,14b,16,17,18(44.8%),β13,14b,16,18(5.9%),β13,14b,17,18(4.0%),β13,14b,16,17,18-short-hub(3.7%)accountedforroughly58.4%ofmappedreadsfromtheCaMKIIβIlluminalibrary.Variantsinwhichexon14a(CAGCCAAGAGTTTAC)followedexon12,suchasβ14a,16,18(13.8%)andβ14a,16,17,18(9.1%),accountedforroughly30%ofmappedreads.Variantsinwhichexon14b(CCAAGAGTTTACTCA)followedexon12,suchasβ14a,16,17,18(7.1%),β14a,16,18(6.3%),andβ14a,17,18(1.2%)accountedforroughly14%ofmappedreads.Secondarypeakscorrespondingtoexons14aand14bareclearlyvisibleinthelast15bpoftheSangersequencingfragment.
certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was notthis version posted August 1, 2019. . https://doi.org/10.1101/721589doi: bioRxiv preprint